Article
Biotechnology & Applied Microbiology
Anqi Chen, Patrick A. Gibney
Summary: By controlling and regulating intracellular trehalose levels, the study aims to improve the survival rate of yeast spores in frozen state. Trehalose plays a crucial role in enhancing the freeze-thaw tolerance of cells, which can aid in the better application of baker's yeast in the frozen baked goods industry.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Cian Holohan, Sophia Hanrahan, Nathan Feely, Peng Li, John O'Connell, Catherine Moss, Michael Carr, Oya Tagit, Gil U. Lee
Summary: The study found that the choice of viral transport media and freeze-thaw cycles significantly impact the sensitivity of COVID-19 molecular diagnostics assays, affecting the stability of the SARS-CoV-2 viral genome. Testing with glycerol-containing PBS is recommended for high stability and sensitivity, as supported by clinical studies on patient-derived samples.
Article
Food Science & Technology
Shi-Yi Wang, Chen Zhang, Qiao-Quan Liu, Zhi-Juan Wang, Ke-Xing Wan, Jian-Ya Qian, Liang Zhang, Chunsen Wu, Qian Li
Summary: Critical melting pretreatment and freeze-thawing treatment were used to modify the structure of potato starch. The pretreatment induced granular swelling and disruption of crystalline structure, resulting in increased viscosity and decreased breakdown. Soluble starch released during the treatments tended to reassociate, improving thermal stability and water and oil absorption of the starch.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Neurosciences
Yiting Wu, Tingwei Song, Qian Xu
Summary: Expansions of short tandem repeats (STRs) are associated with over 50 diseases, particularly neurodegenerative diseases. Several potential molecular mechanisms, including transcriptional silencing, R-LOOP formation, sequestration of RNA-binding proteins, gain-of-function proteins, and repeat-associated non-AUG translation, contribute to STR expansion disorders. R-LOOP, a three-stranded nucleic acid structure formed during transcription, plays a crucial role in inducing DNA damage and genome instability. This review provides a comprehensive discussion on the recent advancements in understanding R-LOOP and its genetic destabilizing effects in neurodegenerative diseases, offering potential targets for drug design and therapeutic advancements.
MOLECULAR NEUROBIOLOGY
(2023)
Article
Pharmacology & Pharmacy
Jayesh Sonje, Carly Fleagle Chisholm, Raj Suryanarayanan
Summary: This study found that the addition of crystallizing excipient mannitol and sugar (sucrose or trehalose) can facilitate ice crystallization, resulting in a homogenous freeze-concentrate with stable protein composition. The use of an annealing step further enhances the stability of the frozen protein solution.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Chemistry, Medicinal
Bruna Minatovicz, Sameera Sansare, Tanu Mehta, Robin H. Bogner, Bodhisattwa Chaudhuri
Summary: This study aimed to determine the individual contributions of freeze-concentration, ice surface area, and processing time to protein destabilization. The ice specific surface area (SSA) was found to increase with an increase in the freezing rate and was also impacted by the position of the sample within the container. The distribution of protein throughout the frozen solution and its stability were enhanced under fast freezing conditions.
JOURNAL OF PHARMACEUTICAL SCIENCES
(2023)
Article
Construction & Building Technology
Milad Shakiba, Amirhossein Hajmoosa, Mussa Mahmoudi, Milad Bazli, Mohsen Ebrahimzadeh
Summary: GFRP stirrups are a promising alternative to steel stirrups due to their non-corrosive nature. This study evaluates their tensile strength under wet-dry and freeze-thaw cycles in seawater.
CONSTRUCTION AND BUILDING MATERIALS
(2023)
Article
Biophysics
Olivier Tytgat, Maarten Fauvart, Tim Stakenborg, Dieter Deforce, Filip Van Nieuwerburgh
Summary: The demand for forensic DNA profiling is increasing, leading to challenges in decentralized workflow due to the need for bulky equipment. Researchers developed a novel hybridization-based STR genotyping method using STRide probes, showing high sensitivity and potential for on-site forensic DNA fingerprinting.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Environmental Sciences
Wei Yuan, Lehua Zhang, Yulong Chang, Hongpeng Ma, Pengbo Fu, Hualin Wang, Jianping Li
Summary: In this study, a combined freezing method of freeze concentration and freeze-thaw demulsification was employed to treat biofuel production wastewater (BPWW) containing complex substances. The results showed that freezing methods could effectively purify water, concentrate pollutants, and separate oil-in-water emulsions, providing a feasible solution for resource recovery and wastewater volume reduction in the biomass-to-energy technology.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Food Science & Technology
Lujie Qin, Yin Fu, Fan Yang, Zhongyi Chang, Chunjing Zou, Hongliang Gao, Deming Jiang, Caifeng Jia
Summary: This study investigated the effect of Flammulina velutipes mycelium polysaccharides (FVP) on the freeze-thaw stability of surimi gels, and found that the addition of FVP during freezing and thawing cycles improved the properties and water holding capacity of the gels.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Food Science & Technology
Di Zhao, Shuqi Liu, He Li, Jun Hao Yoong, Zhiyue Miao, Jinnuo Cao, Xinqi Liu
Summary: In this study, a protein/polysaccharide composite emulsion gel was created by emulsifying soybean isolate protein and palm oil, and incorporating curdlan. The stability of the complex fat substitutes during freeze-thaw at various melting points and palm oil content was investigated. The results showed that the samples with 52 degrees C-palm oil demonstrated the highest freeze-thaw stability.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Chemistry, Applied
Ruoning Zhang, Yanhui Zhang, Jingjing Yu, Yanxiang Gao, Like Mao
Summary: This study developed water-in-oil Pickering emulsions stabilized by ethylcellulose nanoparticles and EC oleogels, which showed improved freeze-thaw stability. Microstructural observation revealed the distribution of EC nanoparticles at the interface and within the water droplets, while the EC oleogel trapped oil in the continuous phase. Freezing and melting temperatures were lowered and enthalpy values reduced with more EC nanoparticles. Freeze-thawing led to decreased water binding capacity but increased oil binding capacity. NMR confirmed increased water mobility but decreased oil mobility after freeze-thawing. Rheological properties showed higher strength and viscosity after freeze-thawing. The widened area of Lissajous plots with more nanoparticles indicated increased viscosity and elasticity of the emulsions.
CARBOHYDRATE POLYMERS
(2023)
Article
Chemistry, Applied
Wen-xin Jiang, Jun-ru Qi, Jin-song Liao, Xiao-quan Yang
Summary: The study proposed an acid-induced pectin gelling mechanism in a 25% ethanol system for constructing emulsion gel system. Differences in structure and properties between HMP and LMP were investigated, with HMP showing superior gelling performance. Molecular weight, neutral sugar side chains, and zeta potential were found to play key roles in the compact gel network construction of HMP. Emulsions with 25% ethanol exhibited gelling properties, and the freeze-thaw stability of pectin emulsions was improved by the stable structure of HMP gel during the cycle.
FOOD HYDROCOLLOIDS
(2021)
Article
Chemistry, Applied
Fan Xie, Xiaolong Ren, Haoming Wu, Hui Zhang, Yan Wu, Zibo Song, Lianzhong Ai
Summary: This study investigated the structural characteristics of pectins with different degrees of esterification and their effects on the properties of corn starch gels. The results showed that low esterification pectin inhibited retrogradation and improved freeze-thaw stability of corn starch gels, while high esterification pectin enhanced the chewiness of starch gels. Additionally, the textural profile of starch gels was mainly influenced by the branching degree, intrinsic viscosity, and DE values of pectin.
FOOD HYDROCOLLOIDS
(2022)
Article
Chemistry, Applied
Fan Xie, Chunmei Yuan, Hui Zhang, Yan Wu, Lianzhong Ai
Summary: This study investigated the structural characteristics of Fenugreek, Guar, Locust bean, and Tara gums and their impact on the physicochemical properties of corn starch gels. The results showed that Fenugreek had a rigid rod structure, while Guar, Locust bean, and Tara gums had an extended structure. The galactomannans with a high Mannose/Galactose ratio and extended structure exhibited higher apparent viscosity, easy gel formation, limited retrogradation, improved freeze-thaw stability, and enhanced textural profile of corn starch gels. These findings are important for improving the sensory characteristics and storage quality of starch-based foods when galactomannans are used.
Article
Genetics & Heredity
Rachel Wiley, Kelly Sage, Bobby LaRue, Bruce Budowle
FORENSIC SCIENCE INTERNATIONAL-GENETICS
(2017)
Article
Genetics & Heredity
Hiromi Brown, Robyn Thompson, Gina Murphy, Dixie Peters, Bobby La Rue, Jonathan King, Anne H. Montgomery, Marion Carroll, James Baus, Sid Sinha, Frank R. Wendt, Bing Song, Ranajit Chakraborty, Bruce Budowle, Sudhir K. Sinha
FORENSIC SCIENCE INTERNATIONAL-GENETICS
(2017)
Review
Biophysics
Alpo Vuorio, Tanja Laukkala, Pooshan Navathe, Bruce Budowle, Robert Bor, Antti Sajantila
AEROSPACE MEDICINE AND HUMAN PERFORMANCE
(2017)
Article
Medicine, Legal
Simone Gittelson, Charles E. H. Berger, Graham Jackson, Ian W. Evett, Christophe Champod, Bernard Robertson, James M. Curran, Duncan Taylor, Bruce S. Weir, Michael D. Coble, John S. Buckleton
FORENSIC SCIENCE INTERNATIONAL
(2018)
Article
Biochemistry & Molecular Biology
Alberto Gomez-Carballa, Jacobo Pardo-Seco, Stefania Brandini, Alessandro Achilli, Ugo A. Perego, Michael D. Coble, Toni M. Diegoli, Vanesa Alvarez-Iglesias, Federico Martinon-Torres, Anna Olivieri, Antonio Torroni, Antonio Salas
Article
Genetics & Heredity
Odile Loreille, Shashikala Ratnayake, Adam L. Bazinet, Timothy B. Stockwell, Daniel D. Sommer, Nadin Rohland, Swapan Mallick, Philip L. F. Johnson, Pontus Skoglund, Anthony J. Onorato, Nicholas H. Bergman, David Reich, Jodi A. Irwin
Article
Genetics & Heredity
John S. Buckleton, Jo-Anne Bright, Kevin Cheng, Bruce Budowle, Michael D. Coble
FORENSIC SCIENCE INTERNATIONAL-GENETICS
(2018)
Article
Genetics & Heredity
John M. Butler, Margaret C. Kline, Michael D. Coble
FORENSIC SCIENCE INTERNATIONAL-GENETICS
(2018)
Review
Genetics & Heredity
Michael D. Coble, Jo-Anne Bright
FORENSIC SCIENCE INTERNATIONAL-GENETICS
(2019)
Article
Genetics & Heredity
Nicole M. M. Novroski, Frank R. Wendt, August E. Woerner, Magdalena M. Bus, Michael Coble, Bruce Budowle
FORENSIC SCIENCE INTERNATIONAL-GENETICS
(2019)
Article
Genetics & Heredity
Michael D. Brandhagen, Odile Loreille, Jodi A. Irwin
Article
Genetics & Heredity
Jo-Anne Bright, Kevin Cheng, Zane Kerr, Catherine McGovern, Hannah Kelly, Tamyra R. Moretti, Michael A. Smith, Frederick R. Bieber, Bruce Budowle, Michael D. Coble, Rashed Alghafrr, Paul Stafford Allen, Amy Barbee, Amy Barbee Vickie Beamer, Christina Buettner, Melanie Russell, Christian Gehrig, Tacha Hicks, Jessica Charak, Kate Cheong-Wing, Anne Ciecko, Christie T. Davis, Michael Donley, Natalie Pedersen, Bill Gartside, Dominic Granger, Marymargaret Greer-Ritzheimer, Erick Reisinger, Jarrah Kennedy, Erin Grammer, Marla Kaplan, David Hansen, Hans J. Larsen, Alanna Laureano, Christina Li, Eugene Lien, Emilia Lindberg, Ciara Kelly, Ben Mallinder, Simon Malsom, Alyse Yacovone-Margetts, Andrew McWhorter, Sapana M. Prajapati, Tamar Powell, Gary Shutler, Kate Stevenson, April R. Stonehouse, Lindsey Smith, Julie Murakami, Eric Halsing, Darren Wright, Leigh Clarks, Duncan A. Taylor, John Buckleton
FORENSIC SCIENCE INTERNATIONAL-GENETICS
(2019)
Article
Genetics & Heredity
Bettina Zimmermann, Kimberly Sturk-Andreaggi, Nicole Huber, Catarina Xavier, Jessica Saunier, Mohammad Tahir, Eliane Chouery, Nadine Jalkh, Andre Megarbane, Martin Bodner, Michael Coble, Jodi Irwin, Thomas Parsons, Walther Parson
FORENSIC SCIENCE INTERNATIONAL-GENETICS
(2019)
Article
Genetics & Heredity
Odile Loreille, Andreas Tillmar, Michael D. Brandhagen, Linda Otterstatter, Jodi A. Irwin
Summary: This study presents an optimized DNA extraction protocol for ultrashort DNA molecules from single rootless hairs. The protocol was successfully applied to samples associated with the Romanov family, providing valuable genetic information and confirming the feasibility of this method for ancient hair samples.
Article
Medicine, Legal
Hannah Kelly, Michael Coble, Maarten Kruijver, Richard Wivell, Jo-Anne Bright
Summary: Relatives share more DNA in common than unrelated people, leading to a higher chance of identical alleles between individuals. The usage of STRmix software allows for the consideration of close biological relatives as alternate sources of DNA, resulting in a more accurate assessment of likelihood ratios. This study investigates the support for siblings of the true contributor to a mixture and analyzes the resulting likelihood ratios.
JOURNAL OF FORENSIC SCIENCES
(2022)
Article
Genetics & Heredity
Amy H. J. Chan, Michael G. Gardner, Adrian Linacre
Summary: We report the detection and visualisation of latent DNA from pangolin scales using a nucleic acid staining dye, and propose that this method is invaluable in wildlife forensic science.
FORENSIC SCIENCE INTERNATIONAL-GENETICS
(2024)
Article
Genetics & Heredity
Fernanda M. Garcia, Raquel S. R. Trabach, Victor S. Stange, Bruno Cancian, Alexia S. S. Zetum, Matheus C. Casotti, Flavia I. V. Errera, Elizeu F. de Carvalho, Debora D. Meira, Iuri D. Louro
Summary: This study found that the population of Espirito Santo, Brazil is genetically closer to the Italian population based on the analysis of STR markers on the X chromosome.
FORENSIC SCIENCE INTERNATIONAL-GENETICS
(2024)
Article
Genetics & Heredity
Zhiyong Liu, Jiajun Liu, Jiaojiao Geng, Enlin Wu, Jianzhang Zhu, Bin Cong, Riga Wu, Hongyu Sun
Summary: Microorganisms can serve as potential markers for identifying body fluids and tissues in forensic genetics. This study explored the use of metatranscriptomics to characterize common forensic samples and investigated the potential application of metatranscriptomics in forensic science. The results demonstrated high alpha diversity in saliva and skin samples, and the use of machine learning models showed that microbial RNA-based methods could be applied for forensic body fluid/tissue identification. Overall, this study provides insights into the role of metatranscriptomics in forensic science and its potential for microbe-based individual identification.
FORENSIC SCIENCE INTERNATIONAL-GENETICS
(2024)
Article
Genetics & Heredity
Guro Dorum, Nadescha Viviane Haenggi, Dario Burri, Yael Marti, Regine Banemann, Galina Kulstein, Cornelius Courts, Annica Gosch, Thorsten Hadrys, Cordula Haas, Jacqueline Neubauer
Summary: RNA has gained attention in forensic field for predicting biological age. This study used RNA-Seq data to search for markers associated with age and presented two candidate marker lists.
FORENSIC SCIENCE INTERNATIONAL-GENETICS
(2024)
Article
Genetics & Heredity
Michele Di Nunzio, Carme Barrot-Feixat, David Gangitano
Summary: This study developed five SNaPshotTM assays for nine chloroplast markers to differentiate between marijuana crop type and hemp crop type. The testing of 122 cannabis samples successfully distinguished between marijuana and hemp, and genetic differences between different groups based on biogeographical origin and crop type were observed. Phylogenetic analysis and principal component analysis provided valuable information about the genetic relationship and differentiation between different cannabis samples.
FORENSIC SCIENCE INTERNATIONAL-GENETICS
(2024)
Article
Genetics & Heredity
Katherine B. Gettings, Martin Bodner, Lisa A. Borsuk, Jonathan L. King, David Ballard, Walther Parson, Corina C. G. Benschop, Claus Borsting, Bruce Budowle, John M. Butler, Kristiaan J. van der Gaag, Peter Gill, Leonor Gusmao, Douglas R. Hares, Jerry Hoogenboom, Jodi Irwin, Lourdes Prieto, Peter M. Schneider, Marielle Vennemann, Christopher Phillips
Summary: The DNA Commission of the ISFG has developed nomenclature recommendations for STR sequences, incorporating research findings and providing guidance for interlaboratory comparisons and the integration of new markers into investigative databases.
FORENSIC SCIENCE INTERNATIONAL-GENETICS
(2024)