Review
Cell Biology
Yuan Liu, Christina Holmes
Summary: This review explores the tissue regeneration potential of EVs isolated from bone marrow and adipose tissue, the two most common clinical sources of adult MSCs, focusing on their angiogenic, osteogenic, and immunomodulatory potentials. Other important issues in the development of MSC-derived EV based therapies are also discussed.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jun Ho Lee, Yu Jin Won, Hail Kim, Minji Choi, Esther Lee, Bumsik Ryoou, Seok-Geun Lee, Byong Seung Cho
Summary: Exosomes derived from adipose tissue-derived stem cells (ASCs) have shown beneficial effects on cell proliferation, migration, and gene expression related to wound healing. Combining these ASC-derived exosomes (ASC-EXOs) with hyaluronic acid (HA) can enhance wound closure rates, tissue regeneration, and collagen production in animal models. This suggests the potential for their use in clinical and regenerative aesthetic applications for skin repair and regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Veterinary Sciences
Usman Rashid, Arfan Yousaf, Muhammad Yaqoob, Evelyn Saba, Muhammad Moaeen-ud-Din, Shahid Waseem, Sandra K. Becker, Gerhard Sponder, Jorg R. Aschenbach, Mansur Abdullah Sandhu
Summary: In this study, we found that canine MSCs derived from infrapatellar fat pad (IPFP) have the highest proliferation rate, while those from omental (OM) adipose tissue have higher cell metabolism. MSCs from all sources showed positive expression of CD73, CD90, CD105, and low expression of CD45. The differentiation potential of canine MSCs isolated from different adipose tissue sources was assessed, with OM-derived MSCs showing better differentiation potential towards adipo- and osteogenic lineages.
BMC VETERINARY RESEARCH
(2021)
Article
Polymer Science
Banafsheh Safari, Marziyeh Aghazadeh, Leila Roshangar, Ayuob Aghanejad, Soodabeh Davaran
Summary: This study reports the fabrication and evaluation of a bioactive hybrid scaffold collagen/P-PCL for bone tissue engineering. The scaffold showed biocompatibility, osteoconductivity, and osteoinductivity, supporting the proliferation and differentiation of AD-MSCs.
EUROPEAN POLYMER JOURNAL
(2022)
Article
Cell & Tissue Engineering
Naoki Ishiuchi, Ayumu Nakashima, Satoshi Maeda, Yoshie Miura, Kisho Miyasako, Kensuke Sasaki, Toshio Uchiki, Ayano Sasaki, Shogo Nagamatsu, Naoki Nakao, Masataka Nagao, Takao Masaki
Summary: Fibrosis is a common histological feature in chronic organ injury, leading to organ failure. Adipose-derived mesenchymal stem cells (ASCs) have been studied as a therapeutic tool to prevent organ failure. This study compared the therapeutic effects of ASCs from superficial and deep layers and found that both types of ASCs are equally effective and safe for treating organ injury.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Engineering, Biomedical
R. Tevlin, H. desJardins-Park, J. Huber, S. E. DiIorio, M. T. Longaker, D. C. Wan
Summary: Osteoarthritis is a global burden that affects joints and bones. Tissue engineering, specifically adipose-derived stromal cells, shows promise as a treatment option. Advancements in material science allow for improved engraftment of these cells and activation of biophysical signals through designed microstructures.
Article
Cell Biology
Furkan Yigitbilek, Sabena M. Conley, Hui Tang, Ishran M. Saadiq, Kyra L. Jordan, Lilach O. Lerman, Timucin Taner
Summary: MSCs from liver and adipose tissue showed similar proliferation and migration properties, but had differences in immunomodulatory and pro-angiogenic abilities. Selecting cell-based therapy according to the specific characteristics of different cell sources may be important for treating certain diseases.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Parisa Khayambashi, Janaki Iyer, Sangeeth Pillai, Akshaya Upadhyay, Yuli Zhang, Simon D. Tran
Summary: Tissue engineering has a long history in regenerative medicine, and methods such as cell-encapsulated hydrogels and exosomes can enhance the regenerative potential of various tissues, with hydrogels being the most user-friendly, economical, and accessible material for encapsulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Medicine, Research & Experimental
Ahmed Abdelbaset-Ismail, Abeer Tharwat, Ahmed E. Ahmed, Tarek Khamis, Ibrahim H. Abd El-Rahim, Sadeq K. Alhag, Mohamed F. Dowidar
Summary: This study found that rat adipose-derived mesenchymal stem cells (rAD-MSCs) could alleviate diclofenac sodium (DIC)-induced acute liver injury by being transplanted into the injured liver. This treatment method significantly improved survival rate, body weight, and liver function indicators, while reducing inflammation and cell damage.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Anatomy & Morphology
Carla Ferreira-Baptista, Andre Queiros, Rita Ferreira, Maria Helena Fernandes, Pedro Sousa Gomes, Bruno Colaco
Summary: This study compared the functional activity and osteogenic potential of adipose tissue-derived stromal cells (ADSCs) isolated from subcutaneous and visceral adipose tissue in cats. The results showed that visceral adipose tissue had a greater osteogenic-inductive capacity, especially when retinoic acid was used.
JOURNAL OF ANATOMY
(2023)
Article
Biochemistry & Molecular Biology
Maria Yoshida, Ayumu Nakashima, Naoki Ishiuchi, Kisho Miyasako, Keisuke Morimoto, Yoshiki Tanaka, Kensuke Sasaki, Satoshi Maeda, Takao Masaki
Summary: Both adipose-derived and bone marrow-derived mesenchymal stem cells (ADSCs and BMSCs) can suppress renal fibrosis, with ADSCs showing a stronger effect under serum-containing culture conditions. However, ADSC infusion in mice may lead to a higher death rate from pulmonary embolism. Therefore, the selection of ADSCs or BMSCs for clinical applications should consider the target disease and culture method.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Medicine, General & Internal
Robert Ossendorff, Alessandra Menon, Frank A. Schildberg, Pietro S. Randelli, Sebastian Scheidt, Christof Burger, Dieter C. Wirtz, Davide Cucchi
Summary: The global distribution and quality of studies using adipose tissue derivates in orthopedics were assessed through a systematic review. Among the 82 included studies, 70% focused on knee disorders, mainly osteoarthritis. Approximately 26% of the studies investigated expanded adipose-derived stem/stromal cells (ADSCs), with 72% of these using stromal vascular fraction (SVF). The overall quality of the studies varied, with a higher score found in studies involving expanded ADSCs compared to SVF.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Orthopedics
Xing Guo, Denghua Huang, Dan Li, Longfei Zou, Hui Lv, Yuanhui Wang, Meiyun Tan
Summary: This study found that hypoxia preconditioning attenuates the proliferation and migration ability of ADSCs, but has positive impact on tenogenic differentiation through HIF-1 alpha signaling pathway.
JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH
(2022)
Article
Cell & Tissue Engineering
Rongmei Qu, Kai He, Tingyu Fan, Yuchao Yang, Liyao Mai, Zhiwei Lian, Zhitao Zhou, Yan Peng, Asmat Ullah Khan, Bing Sun, Xiaolan Huang, Jun Ouyang, Xinghua Pan, Jingxing Dai, Wenhua Huang
Summary: The study used single-cell RNA sequencing to investigate the chemically induced osteogenesis process of human adipose MSCs, identifying some cells intrinsically prepared for osteogenesis and a set of genes related to cell stress response.
Article
Agriculture, Dairy & Animal Science
Byong Seung Cho, Sung-Bae Kim, Sokho Kim, Beomseok Rhee, Jungho Yoon, Jae Won Lee
Summary: In this study, the effects of canine ASC-exosomes on canine AD were investigated using a Biostir-induced AD mouse model. The study found that cASC-EVs improved AD-like dermatitis and decreased serum IgE, ear thickness, and inflammatory cytokines such as IL-4 and IFN-γ in a dose-dependent manner. In vivo toxicity studies showed no systemic toxicity. miRNA analysis showed the presence of anti-inflammatory miRNAs in cASC-EVs, suggesting a promising cell-free therapy for treating canine AD.