Journal
FOOD SCIENCE AND BIOTECHNOLOGY
Volume 22, Issue 6, Pages 1481-1486Publisher
KOREAN SOCIETY FOOD SCIENCE & TECHNOLOGY-KOSFOST
DOI: 10.1007/s10068-013-0241-3
Keywords
Cirsium setidens Nakai; marker compound; flavone; HPLC-DAD; validation
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Funding
- Globalization of Korean Foods RD program
- Ministry of Food, Agriculture, Forestry and Fisheries, Republic of Korea [911027-1]
- Korea Food Research Institute
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An effective method of high performance liquid chromatography-diode array detector (HPLC-DAD) was developed for the simultaneous quantification of two marker compounds, pectolinarin and pectolinarigenin, in Cirsium setidens Nakai, a Korean wild herb. The two marker compounds of C. setidens were separated on a C18 column with a gradient system. The calibration curves of the two standard compounds exhibited good linearity (R-2 >0.9992). The limits of detection (LOD) values of pectolinarin and pectolinarigenin were 0.52 and 0.41 mu g/mL, respectively, while their limits of quantification (LOQ) values were 1.57 and 1.25 mu g/mL, respectively. The relative standard deviations (RSDs) of the data of the intra- and inter-day experiments were less than 1.65 and 2.78%, respectively. The results of the recovery test were 98.06-105.81% with an RSD value of 1.87-3.50%. In conclusion, the results of validation showed that the proposed BPLC method was useful for the quantification of the two marker compounds in C. setidens.
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