Journal
FOOD SCIENCE AND BIOTECHNOLOGY
Volume 19, Issue 6, Pages 1655-1659Publisher
KOREAN SOCIETY FOOD SCIENCE & TECHNOLOGY-KOSFOST
DOI: 10.1007/s10068-010-0234-4
Keywords
loop-mediated isothermal amplification (LAMP); Salmonella; rapid detection; food-borne pathogen; invA
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Funding
- Science Foundation of Ministry of Education of China [706046]
- National Natural Science Foundation of China [20436020]
- National Science Foundation for Distinguished Young Scholars of China [31000781]
- China Scholarship Council [2008615044]
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A loop-mediated isothermal amplification (LAMP) method for rapid detection of the food-borne Salmonella strains had been developed and evaluated in this study. The optimal reaction condition was found to be 65 degrees C for 45 min, with the detection limit as 1 pg DNA/tube and 100 CFU/reaction. Application of LAMP assays was performed on 214 food-borne Salmonella strains using a rapid procedure and easy result confirmation, where the specificity of LAMP and polymerase chain reaction (PCR) assays was 97.7% (209/214) and 91.6% (196/214), respectively; with a 100% specificity for both assays.
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