☆ 4.4 Article

Rapid Detection of Vibrio parahaemolyticus Strains and Virulent Factors by Loop-mediated Isothermal Amplification Assays

FOOD SCIENCE AND BIOTECHNOLOGY (2010)

Journal

FOOD SCIENCE AND BIOTECHNOLOGY

Volume 19, Issue 5, Pages 1191-1197

Publisher

KOREAN SOCIETY FOOD SCIENCE & TECHNOLOGY-KOSFOST

DOI: 10.1007/s10068-010-0170-3

Keywords

loop-mediated isothermal amplification (LAMP); Vibrio parahaemolyticus; rapid detection

Categories

Food Science & Technology

Funding

Science Foundation of Ministry of Education of China [706046]
National Natural Science Foundation of China [20436020]
China Scholarship Council [2008615044]

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Abstract

A loop-mediated isothermal amplification (LAMP) method for rapid detection of the foodborne Vibrio parahaemolyticus strains and related virulent factors had been developed and evaluated in this study. Six primers, including outer primers, inner primers, and loop primers, were specially designed for recognizing 8 distinct sequences on 3 target genes, which were tlh, tdh, and trh. The detection limits were found to be 100, 100 fg, and 1 pg DNA/tube for tlh, tdh, and trh, respectively. Application of LAMP assays were performed on 368 foodborne V parahaemolyticus strains, the sensitivities of LAMP assays for the Ilk tdh, and trh were 100, 95.6, and 96.4%, and the negative predictive values (NPV) were 100, 84.7, and 93.1%, respectively; with a 100% specificity and positive predictive value (PPV) for all 3 target genes.

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