Wine yeast molecular typing using a simplified method for simultaneously extracting mtDNA, nuclear DNA and virus dsRNA

Quick and accurate methods are required for the identification of industrial, environmental, and clinical yeast strains We propose a rapid method for the simultaneous extraction of yeast mtDNA, nuclear DNA, and virus dsRNA It is simpler. cheaper, and faster than the previously reported methods It allows one to choose among a broad range of molecular analysis approaches for yeast typing, avoiding the need to use of several different Methods for the separate extraction of each nucleic acid type The application of this method followed by the combined analysis of mtDNA and dsRNA (ScV-M and W) is a highly attractive option for fast and efficient wine yeast typing (C) 2009 Elsevier Ltd All rights reserved