Journal
FOOD CHEMISTRY
Volume 164, Issue -, Pages 98-103Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2014.05.036
Keywords
Rhodamine B; HPLC-FLD; Illegal dyes; Food safety; Chili
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In this work, a simple, rapid and sensitive analytical method for the determination of rhodamine B in chili-containing foodstuffs is described. The dye is extracted from samples with methanol and analysed without further cleanup procedure by high-performance liquid chromatography (HPLC) coupled to fluorescence detection (FLD). The influence of matrix fluorescent compounds (capsaicin and dihydrocapsaicin) on the analysis was overcome by the optimisation of mobile-phase composition. The limit of determination (LOD) and limit of quantification (LOQ) were 3.7 and 10 mu g/kg, respectively. Validation data show a good repeatability and within-lab reproducibility with relative standard deviations <10%. The overall recoveries are in the range of 98-103% in chili powder and in the range of 87-100% in chili oil depending on the concentration of rhodamine B in foodstuffs. This method is suitable for the routine analysis of rhodamine B due to its sensitivity, simplicity, reasonable time and cost. (C) 2014 Elsevier Ltd. All rights reserved.
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