Journal
FOOD CHEMISTRY
Volume 141, Issue 3, Pages 2522-2525Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2013.05.085
Keywords
Baihuasheshecao; Hedyotis corymbosa; Hedyotis diffusa; Internal transcribed spacer (ITS); Loop-mediated isothermal amplification (LAMP); Molecular authentication
Funding
- Innovation and Technology Fund, Innovation and Technology Commission, Hong Kong SAR [ITS/162/11]
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Hedyotis diffuse Willd. (Baihuasheshecao) is an ingredient of herbal teas commonly consumed in the Orient and tropical Asia for cancer treatment and health maintenance. In the market, this ingredient is frequently adulterated by the related species Hedyotis corymbosa (L) Lam. The objective of this study is to develop a novel loop-mediated isothermal amplification (LAMP) technique to differentiate H. diffuse from its adulterant H. colymbosa. A set of four internal control primers (F3, FIP, BIP and B3) were designed based on six loci in the internal transcribed spacer (ITS) for LAMP of both H. diffuse and H. corymbosa. Two specific primers (S_F3 and S_FIP) were designed for specific LAMP detection of H. diffusa only. Our data showed that LAMP was successful for both H. diffuse and H. corymbosa in internal control. In contrast, only H. diffuse was detected in specific LAMP using the specific primers S_F3 and S_FIP. This study showed that LAMP was useful to differentiate H. diffuse from its adulterant H. corymbosa. This study is significant for the verification of the authenticity for better quality control of this common herbal tea ingredient. The strategy of including an internal control assures the quality of the concerned DNA region for LAMP. (C) 2013 Elsevier Ltd. All rights reserved.
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