Journal
FOOD CHEMISTRY
Volume 139, Issue 1-4, Pages 845-852Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2013.01.088
Keywords
Alkaloids; Identification; Quantification; Liquid chromatography-ion trap mass spectrometry
Funding
- National Scientific Foundation of China [90817103, 20775055, 30973672]
- Important National Science and Technology Specific Projects [2009ZX09301-14]
- Fundamental Research Funds for the Central Universities
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Catharanthus roseus is an important dicotyledonous medicinal plant that produces anticancer compounds. The active alkaloids vinblastine, vindoline, ajmalicine, catharanthine, and vinleurosine were identified by direct-injection ion trap-mass spectrometry (IT-MS) for collecting MS1-2 spectra. The determinations of five alkaloids were accomplished by liquid chromatography (LC) with UV and MS detections. The analytes provided good signals corresponding to the protonated molecular ions [M+H](+) and product ions. The precursor ions and product ions for quantification of vinblastine, vindoline, ajmalicine, catharanthine, and vinleurosine were m/z 825 -> 807, 457 -> 397, 353 -> 144, 337 -> 144 and 809 -> 748 by LC-IT-MS, respectively. Two methods were used to evaluate a number of validation characteristics (repeatability, LOD, calibration range, and recovery). MS provided a high selectivity and sensitivity for determination of five alkaloids in positive mode. After optimisation of the methods, separation, identification and quantification of the five components in C. roseus were comprehensively accomplished by HPLC with UV and MS detection. (c) 2013 Elsevier Ltd. All rights reserved.
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