Journal
FOOD CHEMISTRY
Volume 135, Issue 4, Pages 2326-2332Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2012.07.062
Keywords
Isoamyl acetate; Candida rugosa lipase; Porcine pancreatic lipase; Calcium alginate; Chitosan; Immobilisation
Funding
- Scientific and Technical Research Council of Turkey, TUBITAK [107T919]
- Scientific Research Units of the Mustafa Kemal University [09M 0501]
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Candida rugosa lipase (CRL) and porcine pancreatic lipase (PPL) were immobilised by entrapping and also by covalent binding for use in synthesis of isoamyl acetate (IAAc), which has a typical banana flavour. Lipase entrapment was carried out by dripping sodium alginate (Na-Alg)-chitosan (Chi)-lipase mixture into CaCl2-glutaraldehyde (GAL) solution to obtain Ca-Alg/Chi(CRL/PPL). Immobilisation conditions were optimised as 1.5% Na-Alg, 1.5% chitosan and 0.15% GAL Ca-Alg/Chi(CRL/PPL) samples showed the highest activity when they were dried upon reaching 27% of their initial weights. Covalent binding was achived with Chi modified with spacerarm via glutaraldehyde to get Chi(CRL/PPL). The highest IAAc production was observed when 1,3-diaminopropane was used as a spacer arm. The best ester yield was achieved in heptane, at 40 and 45 degrees C reaction temperatures, 50 mM IAA and 50 or 75 mM AA concentrations. The amount of IAAc was nearly 10 times higher for the batch type than for the continuous packed bed column reactor. (C) 2012 Elsevier Ltd. All rights reserved.
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