Journal
FOOD CHEMISTRY
Volume 129, Issue 4, Pages 1710-1720Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2011.06.036
Keywords
Mangosteen pericarp; Condensed tannins; Antioxidant activity; Fractionation; Degree of polymerization; Structure-activity
Funding
- National Natural Science Foundation of China [31070522]
- Program for New Century Excellent Talents in University [NCET-07-0725]
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The extract from mangosteen pericarp was separated into eight fractions, four by solvent extraction (hexane, FH; petroleum ether, FP; ethyl acetate, FE; water, FW) and the other four by fractionation on Sephadex LH-20 column (fractions F1, F2, F3, and F4). Phenolic compounds were identified as the major antioxidant components in each fraction. UV-VIS spectrum, reversed-phase HPLC-ESI-MS coupled with thiolysis, normal-phase HPLC-ESI-MS and MALDI-TOF-MS analyses complementally showed that the phenolic-rich fractions were condensed tannins with structural heterogeneity in monomer units, degree of polymerization and interflavan linkages. Mangosteen condensed tannins predominately contained procyanidins with a significant amount of propelargonidin but much lower signals of prodelphinidin. Eicosapentamers of condensed tannins were detected by MALDI-TOF-MS. Both B-type and A-type linkages were present. Condensed tannins fractions from mangosteen pericarp, especially fractions F3 and FE, can be explored as beneficial food antioxidants because of their high yields and potent antioxidant activities. (C) 2011 Elsevier Ltd. All rights reserved.
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