Qualitative and quantitative analysis of curcuminoids in herbal medicines derived from Curcuma species

A validated and sensitive HPLC-UV-MS method was developed for qualitative and quantitative analysis of curcuminoids in eight herbal medicines derived from four Curcuma species. The samples were separated on a YMC ODS-A C-18 column with a gradient elution of acetonitrile and 0.1% formic acid. Curcumin, demethoxycurcumin and bisdemethoxycurcumin showed good linearity (r > 0.9998) in the concentration ranges of 4.88-625, 4.29-550 and 3.98-510 mu g/mL, respectively. The results suggested that the contents of three major curcuminoids in different herbal medicines varied significantly. Curcuminoids were only detected in Jianghuang, HuangsiYujin, and PengEzhu. Amongst them, Jianghuang contained the highest amounts of curcuminoids (40.36 mg/g), which were almost 20 times higher than HuangsiYujin (1.94 mg/g) and 400 times higher than PengEzhu (0.098 mg/g). Furthermore, amongst the Jianghuang samples collected from different areas, samples from Sichuan Province contained remarkably higher amounts of curcuminoids (22.21-40.36 mg/g) than other cultivation regions. (c) 2010 Elsevier Ltd. All rights reserved.