SYBR®GreenER™ Real-Time PCR to detect almond in traces in processed food

Because food ingredients are sometimes considered as causative factors in IgE mediated food allergies, DNA-based tests may prove to be very useful to establish whether allergenic species have been used in foodstuffs production. The development of two SYBR (R) GreenER (TM) Real-Time PCR assays. targeting Pru 1 and rbcL genes, based on melting curve analysis, to detect allergen species in food has been presented. Applicability of these methods was assessed with several commercial products containing processed almond. (C) 2009 Elsevier Ltd. All rights reserved.