Article
Agriculture, Dairy & Animal Science
Tomoko Harayama, Takamitsu Tsukahara, Kikuto Fukuta, Machi Oda, Ryo Inoue
Summary: This study measured immunocytes in piglets to investigate how their populations change during development and are affected by weaning. The results showed a decrease in myeloid cells and an increase in NK cells after birth, while killer T, helper T, and B cell populations increased around 2-3 weeks after birth. There were no significant differences in the populations of the evaluated cell types between suckling and weaned piglets for at least 14 days post weaning.
Article
Chemistry, Multidisciplinary
Shuai Liu, Xu Wang, Xueliang Yu, Qiang Cheng, Lindsay T. Johnson, Sumanta Chatterjee, Di Zhang, Sang M. Lee, Yehui Sun, Ting-Chih Lin, John L. Liu, Daniel J. Siegwart
Summary: The study introduces a rational design and combinatorial synthesis of zwitterionic phospholipidated polymers (ZPPs) for efficient mRNA delivery to spleen and lymph nodes, with potential for substantial immunotherapeutic applications.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Pharmacology & Pharmacy
Reyaj Mikrani, Ian K. Styles, Thu A. Hoang, Mohammad Abdallah, Danielle Senyschyn, Christopher J. H. Porter, Enyuan Cao, Natalie L. Trevaskis
Summary: Mesenteric lymph leakage associated with obesity diverts dietary lipids, drugs, and antigens away from their normal lymphatic trafficking pathways, contributing to detrimental changes in lipid metabolism and immunity.
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2022)
Article
Critical Care Medicine
Hua Liu, Jian-Feng Li, Li-Min Zhang, Huai-Huai Wang, Xu-Qing Wang, Gui-Qing Liu, Hui-Bo Du, Yu-Jie Jin, Li-Qiang Xing, Zi-Gang Zhao, Chun-Yu Niu
Summary: This study found that post-hemorrhagic shock mesenteric lymph (PHSML) has adverse effects on splenic dendritic cells (DCs), contributing to immune dysfunction in severe hemorrhagic shock. PHSML drainage attenuated cell death and enhanced the response of DCs to LPS stimulation, promoting cytokine production and surface marker expressions. These findings suggest that PHSML plays a crucial role in hemorrhagic shock-induced immunosuppression through impairing DC function and maturation.
Article
Food Science & Technology
Unni Cecilie Nygaard, Ashild Vege, Torleiv Rognum, Koni Grob, Christel Cartier, Jean-Pierre Cravedi, Jan Alexander
Summary: Humans are exposed to mineral oil saturated hydrocarbons (MOSH) from the diet. Female rats were exposed to different fractions of MOSH and showed increased liver and spleen weight, vacuolization, and granuloma formation. The effects varied depending on the MOSH fractions tested and were related to the accumulation of specific types of hydrocarbons. The accumulation of iso-alkanes and substituted cycloalkanes in rat tissues were similar to those in humans.
FOOD AND CHEMICAL TOXICOLOGY
(2023)
Article
Gastroenterology & Hepatology
Yuya Umebayashi, Satoru Muro, Masanori Tokunaga, Toshifumi Saito, Yuya Sato, Toshiro Tanioka, Yusuke Kinugasa, Keiichi Akita
Summary: Through cadaveric studies, it was found that there are more posterior lymph nodes in the splenic hilum area and they are located in the deep intervascular area, which may be neglected during spleen-preserving splenic hilar lymph node dissection (SPSHLD). Surgeons should consider the possibility of residual posterior lymph nodes in SPSHLD.
WORLD JOURNAL OF GASTROINTESTINAL SURGERY
(2023)
Review
Biochemical Research Methods
Gregory Bauwens, Aleksandra Gorska, Giorgia Purcaro
Summary: Mineral oil hydrocarbons (MOH) are analyzed using the online coupled liquid chromatography-gas chromatography with flame ionization detection (LC-GC-FID) method, which lacks sufficient separation performance and may result in coelution of interfering compounds. Comprehensive two-dimensional gas chromatography (GC x GC) has proven to be relevant in overcoming the weaknesses of the LC-GC method and meeting the analytical requirements. This paper highlights the contributions of GC x GC in understanding MOH, meeting the determination requirements, and overcoming analytical and toxicological challenges.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Gregory Bauwens, Sebastiano Panto, Giorgia Purcaro
Summary: The determination of mineral oil contamination levels in foods is a complex issue that requires methods like GC x GC for data interpretation and reliable integration of analytical results. The application of a new algorithm on an automated platform provides a novel solution for quantification and highlights the advantages of using an integrated platform.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Food Science & Technology
Raquel B. Gomez-Coca, Maria Del Carmen Perez-Camino, Wenceslao Moreda
Summary: The aim of this study was to determine the content of mineral oil hydrocarbons (MOH) in olive pomace oil and contribute to the monitoring required by EFSA. The study provided information on the concentrations and molecular mass profiles of MOH, as well as identified the structures that prove the harmless nature of the mineral oil aromatic hydrocarbon (MOAH) fraction. The results showed that the C25-C35 fraction is the main fraction to which humans are exposed through olive pomace oil, with concentrations dependent on the extraction process.
Article
Food Science & Technology
Dieter Schrenk, Margherita Bignami, Laurent Bodin, Jesus del Mazo, Bettina Grasl-Kraupp, Christer Hogstrand, Laurentius (Ron) Hoogenboom, Jean-Charles Leblanc, Carlo Stefano Nebbia, Elsa Nielsen, Evangelia Ntzani, Annette Petersen, Salomon Sand, Tanja Schwerdtle, Christiane Vleminckx, Heather Wallace, Jan Alexander, Christophe Goldbeck, Konrad Grob, Jose angel Gomez Ruiz, Olaf Mosbach-Schulz, Marco Binaglia, James Kevin Chipman
Summary: Mineral oil hydrocarbons (MOH) consist of saturated hydrocarbons (MOSH) and aromatic hydrocarbons (MOAH). The complex composition of MOH makes it impossible to fully characterize chemically. Accumulation of MOSH differs in various tissues among different species. Liver damage and inflammation are observed in F344 rats due to specific accumulation of wax components, while no such effects are seen in SD rats or humans. Based on dietary exposure assessments, the present dietary exposure to MOSH is not a concern for human health. However, the presence of genotoxic and carcinogenic MOAH with three or more aromatic rings raises possible concerns for human health. The highest dietary exposure to MOAH is found in the young population. Overall, this article highlights the importance of understanding the effects and risks associated with the different components of mineral oil hydrocarbons. Rating: 8/10.
Article
Agriculture, Dairy & Animal Science
Elettra Febo, Francesca Del Signore, Nicola Bernabo, Andrea Paolini, Francesco Simeoni, Andrea De Bonis, Martina Rosto, Sara Canal, Massimo Vignoli
Summary: This study evaluated the diagnostic performance of strain elastography (SE) in differentiating between benign and malignant lymph nodes in cats, using a multivariate approach that included SE results, B-mode ultrasound, and color Doppler findings. The results showed that by combining these different imaging techniques, it was possible to accurately distinguish between the three groups of lymph nodes.
Article
Biochemistry & Molecular Biology
Basak B. Ural, Daniel P. Caron, Pranay Dogra, Steven B. Wells, Peter A. Szabo, Tomer Granot, Takashi Senda, Maya M. L. Poon, Nora Lam, Puspa Thapa, Yoon Seung Lee, Masaru Kubota, Rei Matsumoto, Donna L. Farber
Summary: Carbon-containing particulates accumulate in lung-associated lymph nodes with age, leading to decreased macrophage function and disruption of lymphoid tissue structure, which may compromise respiratory immune responses.
Article
Engineering, Manufacturing
Romy Fengler, Ludwig Gruber
Summary: This article compares migration studies of mineral oil hydrocarbons (MOH) from real papers with previous studies on surrogates and food simulants. The results show that the migration behavior of MOH can be depicted by the use of respective surrogates, which helps to simplify the analytical tasks. The study also verifies the applicability of food simulants in contact with MOH.
PACKAGING TECHNOLOGY AND SCIENCE
(2022)
Article
Multidisciplinary Sciences
Coralie M. Backlund, Rebecca L. Holden, Kelly D. Moynihan, Daniel Garafola, Charlotte Farquhar, Naveen K. Mehta, Laura Maiorino, Sydney Pham, J. Bryan Iorgulescu, David A. Reardon, Catherine J. Wu, Bradley L. Pentelute, Darrell J. Irvine
Summary: Covalent conjugation of antigens with cell-penetrating peptides enhances the potency of peptide vaccines by promoting antigen uptake by dendritic cells and accumulation in draining lymph nodes. This results in robust T cell priming and expansion.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Oncology
Chunhui Jiang, Ye Liu, Chunjie Xu, Yanying Shen, Qing Xu, Lei Gu
Summary: The study aimed to explore the pathological characteristics of lymph nodes around inferior mesenteric artery in rectal cancer, and found that the distance from anal verge, CEA level, differentiation, and T stage were independent risk factors for positive lymph nodes. Therefore, choosing appropriate surgical methods is crucial for better oncological outcomes and reduced postoperative complications.
WORLD JOURNAL OF SURGICAL ONCOLOGY
(2021)
Article
Oncology
Nicole Concin, Xavier Matias-Guiu, Ignace Vergote, David Cibula, Mansoor Raza Mirza, Simone Marnitz, Jonathan Ledermann, Tjalling Bosse, Cyrus Chargari, Anna Fagotti, Christina Fotopoulou, Antonio Gonzalez Martin, Sigurd Lax, Domenica Lorusso, Christian Marth, Philippe Morice, Remi A. Nout, Dearbhaile O'Donnell, Denis Querleu, Maria Rosaria Raspollini, Jalid Sehouli, Alina Sturdza, Alexandra Taylor, Anneke Westermann, Pauline Wimberger, Nicoletta Colombo, Francois Planchamp, Carien L. Creutzberg
Summary: The European consensus conference on endometrial carcinoma in 2014 aimed to produce multi-disciplinary evidence-based guidelines. Since then, the European Society of Gynaecological Oncology (ESGO), the European SocieTy for Radiotherapy and Oncology (ESTRO), and the European Society of Pathology (ESP) have decided to update the guidelines and cover new topics to improve care for women with endometrial carcinoma globally.
INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER
(2021)
Article
Hematology
Charles Herbaux, Christoph Kornauth, Stephanie Poulain, Stephen J. F. Chong, Mary C. Collins, Rebecca Valentin, Liam Hackett, Olivier Tournilhac, Francois Lemonnier, Jehan Dupuis, Adrien Daniel, Cecile Tomowiak, Kamel Laribi, Loic Renaud, Damien Roos-Weil, Cedric Rossi, Eric Van den Neste, Cecile Leyronnas, Fatiha Merabet, Jean Valere Malfuson, Mourad Tiab, Loic Ysebaert, Samuel Ng, Franck Morschhauser, Philipp B. Staber, Matthew S. Davids
Summary: Novel agent classes like JAK/STAT and T-cell receptor pathway inhibitors, as well as HDAC inhibitors, have shown preclinical activity in T-PLL. BH3 profiling revealed that primary T-PLL cells mainly depend on BCL-2 and MCL-1 proteins for survival. The combination therapy of venetoclax and ruxolitinib demonstrated significant efficacy in refractory T-PLL patients, especially in those with JAK3 mutations.
Letter
Hematology
Christoph Kornauth, Charles Herbaux, Bernd Boidol, Chantal Guillemette, Patrick Caron, Marius E. Mayerhoefer, Stephanie Poulain, Olivier Tournilhac, Tea Pemovska, Stephen J. F. Chong, Emiel van der Kouwe, Lukas Kazianka, Georg Hopfinger, Daniel Heintel, Roland Jaeger, Markus Raderer, Ulrich Jager, Ingrid Simonitsch-Klupp, Wolfgang R. Sperr, Stefan Kubicek, Matthew S. Davids, Philipp B. Staber
Article
Oncology
Joanna Baum, Elena Ioana Braicu, Oliver Hunsicker, Ignace Vergote, Nicole Concin, Els Van Nieuwenhuysen, Aarne Feldheiser, Patriciu Achimas-Cadariu, Silvia Darb-Esfahani, Astrid Berger, Bogdan Fetica, Sven Mahner, Andrea Papadia, Linn Woelber, Maria Luisa Gasparri, Adriaan Vanderstichele, Pierluigi Benedetti Panici, Michael D. Mueller, Ilary Ruscito, Hannah Woopen, Jalid Sehouli
Summary: This study aimed to identify characteristics of long-term survivors with high-grade serous ovarian cancer and found that platinum sensitivity, recurrence, ascites, and BRCA1 gene mutation were significant factors affecting long-term survival. Intraoperatively, long-term survivors also had less tumor involvement of the upper abdomen.
INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER
(2021)
Article
Pathology
Nicole Concin, Carien L. Creutzberg, Ignace Vergote, David Cibula, Mansoor Raza Mirza, Simone Marnitz, Jonathan A. Ledermann, Tjalling Bosse, Cyrus Chargari, Anna Fagotti, Christina Fotopoulou, Antonio Gonzalez-Martin, Sigurd F. Lax, Domenica Lorusso, Christian Marth, Philippe Morice, Remi A. Nout, Dearbhaile E. O'Donnell, Denis Querleu, Maria Rosaria Raspollini, Jalid Sehouli, Alina E. Sturdza, Alexandra Taylor, Anneke M. Westermann, Pauline Wimberger, Nicoletta Colombo, Francois Planchamp, Xavier Matias-Guiu
Summary: A European consensus conference on endometrial carcinoma was held in 2014 to produce multidisciplinary evidence-based guidelines. Given the large body of literature on the management of endometrial carcinoma published since 2014, the guidelines were updated by ESGO/ESTRO/ESP. The guidelines cover various aspects of endometrial carcinoma care and are evidence-based.
Article
Hematology
E. van der Kouwe, G. Heller, A. Czibere, J. A. Pulikkan, C. Agreiter, L. H. Castilla, R. Delwel, A. Di Ruscio, A. K. Ebralidze, M. Forte, F. Grebien, E. Heyes, L. Kazianka, J. Klinger, C. Kornauth, T. Le, K. Lind, I. A. M. Barbosa, T. Pemovska, A. Pichler, A-S Schmolke, C. M. Schweicker, H. Sill, W. R. Sperr, A. Spittler, S. Surapally, B. Q. Trinh, P. Valent, K. Vanura, R. S. Welner, J. Zuber, D. G. Tenen, P. B. Staber
Summary: The study reveals that downregulation of PU.1 in the blood system is an active process induced by an antisense promoter driven by RUNX transcription factors, resulting in myeloid differentiation blockade. Patients with CBF-AML show an elevated antisense/sense transcript ratio and promoter accessibility compared to normal karyotype AML or healthy CD34(+) cells. Additionally, competitive interaction between an enhancer and the proximal or antisense promoter acts as a binary switch for myeloid or T-cell development.
Article
Oncology
Jakob Nedomansky, Werner Haslik, Ursula Pluschnig, Christoph Kornauth, Christine Deutschmann, Stefan Hacker, Guenther G. Steger, Rupert Bartsch, Robert M. Mader
Summary: Analyzing removed tissue from patients undergoing surgical intervention for extravasation of anthracyclines revealed individual variations in tissue sensitivity, with surgical intervention post-demarcation leading to healing within approximately 2 weeks. Despite high variability in tissue concentrations post-extravasation surgery, preventive measures remain crucial in preventing tissue necrosis.
CANCER CHEMOTHERAPY AND PHARMACOLOGY
(2021)
Letter
Oncology
Nicole Concin, Xavier Matias-Guiu, Carien L. Creutzberg
INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER
(2021)
Article
Oncology
Charlotte Maene, Rawand Rokan Salihi, Els Van Nieuwenhuysen, Sileny N. Han, Nicole Concin, Ignace Vergote
Summary: This study found that the addition of bevacizumab to weekly paclitaxel and carboplatin in neoadjuvant treatment of cervical cancer stage IB-IIB resulted in a 100% radiological RECIST response and an optimal pathological response rate of 38%. Despite the established role of bevacizumab in combination with paclitaxel and carboplatin in recurrent cervical cancer, no superior effect on pathological response rate was observed in the neoadjuvant chemotherapy setting.
INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER
(2021)
Article
Oncology
Christoph Kornauth, Tea Pemovska, Gregory Vladimer, Gunther Bayer, Michael Bergmann, Sandra Eder, Ruth Eichner, Martin Erl, Harald Esterbauer, Ruth Exner, Verena Felsleitner-Hauer, Maurizio Forte, Alexander Gaiger, Klaus Geissler, Hildegard T. Greinix, Wolfgang Gstoettner, Marcus Hacker, Bernd Lorenz Hartmann, Alexander W. Hauswirth, Tim Heinemann, Daniel Heintel, Mir Alireza Hoda, Georg Hopfinger, Ulrich Jaeger, Lukas Kazianka, Lukas Kenner, Barbara Kiesewetter, Nikolaus Krall, Gerhard Krajnik, Stefan Kubicek, Trang Le, Simone Lubowitzki, Marius E. Mayerhoefer, Elisabeth Menschel, Olaf Merkel, Katsuhiro Miura, Leonhard Muellauer, Peter Neumeister, Thomas Noesslinger, Katharina Ocko, Leopold Oehler, Michael Panny, Alexander Pichler, Edit Porpaczy, Gerald W. Prager, Markus Raderer, Robin Ristl, Reinhard Ruckser, Julius Salamon, Ana-Iris Schiefer, Ann-Sofie Schmolke, Ilse Schwarzinger, Edgar Selzer, Christian Sillaber, Cathrin Skrabs, Wolfgang R. Sperr, Ismet Srndic, Renate Thalhammer, Peter Valent, Emiel van der Kouwe, Katrina Vanura, Stefan Vogt, Cora Waldstein, Dominik Wolf, Christoph C. Zielinski, Niklas Zojer, Ingrid Simonitsch-Klupp, Giulio Superti-Furga, Berend Snijder, Philipp B. Staber
Summary: Personalized medicine using functional assays can be clinically feasible and effective in providing treatment guidance for patients with aggressive hematologic cancers, resulting in enhanced progression-free survival and exceptional responses lasting longer than expected for some patients.
Article
Oncology
Daniela Berger, Karin Bauer, Christoph Kornauth, Susanne Gamperl, Gabriele Stefanzl, Dubravka Smiljkovic, Christian Sillaber, Peter Bettelheim, Paul Knoebl, Ana-Iris Schiefer, Georg Greiner, Renate Thalhammer, Gregor Hoermann, Ilse Schwarzinger, Philipp B. Staber, Wolfgang R. Sperr, Peter Valent
Summary: Secondary basophilic leukemia is a rare type of leukemia characterized by significant expansion of basophils during disease progression, with a generally poor prognosis. Monotherapy with azacitidine may not be sufficient to control the disease effectively, and combination therapy may offer potential for better outcomes. Future clinical studies are needed to determine the efficacy of drug combinations, such as venetoclax, in improving outcomes for these patients.
Article
Food Science & Technology
Laura Barp, Sabrina Moret, Giorgia Purcaro
Summary: Polycyclic aromatic hydrocarbons (PAHs) are common environmental and processing contaminants generated by organic matter combustion processes. The contamination of PAHs in vegetable oils can come from various sources, including environmental pollution, oil processing, and migration from food contact materials. A purification method using solid-phase extraction (SPE) and high performance liquid chromatography-fluorometric detector (HPLC-FLD) analysis was developed to isolate PAHs from the complex matrix of edible oil. Evaluation of the pomace oil supply chain revealed an increase in PAH contamination with storage time and a significant contribution of the pomace drying process to the total PAH content.
Article
Biochemistry & Molecular Biology
Ana Srbinovska, Paolo Lucci, Chiara Conchione, Laura Barp, Sabrina Moret
Summary: The potential of endogenous n-alkane profiling for assessing EVOO adulteration has been studied by few authors. A rapid and solvent-sparing offline SPE-GC-FID method was optimized and validated for determining endogenous n-alkanes in vegetable oils. Promising indices were found to reveal the addition of SFO in EVOO and AVO in EVOO.
Review
Food Science & Technology
Laura Barp, Ana Miklavcic Visnjevec, Sabrina Moret
Summary: Pressurized liquid extraction (PLE) is an advanced extraction technique aimed at saving time and reducing solvent in comparison to traditional extraction processes. It is commonly used for solid and semi-solid samples, employing solvent extraction at elevated temperatures and pressures. The use of specific pressure and temperature conditions changes the physicochemical properties of the extraction solvent, allowing deeper penetration into the matrix to be extracted. By combining extraction and clean-up steps, PLE becomes a versatile and selective technique. This review focuses on recent applications of PLE in the field of food contaminants.
Article
Multidisciplinary Sciences
Tea Pemovska, Johannes Bigenzahn, Ismet Srndic, Alexander Lercher, Andreas Bergthaler, Adrian Cesar-Razquin, Felix Kartnig, Christoph Kornauth, Peter Valent, Philipp B. Staber, Giulio Superti-Furga
Summary: Metabolic reprogramming plays a critical role in cancer development. The study demonstrates the use of a metabolic drug library to uncover metabolic vulnerabilities and gain functional insights into myeloid leukemia biology. High-throughput screening approaches are effective in identifying cancer-specific metabolic vulnerabilities and unraveling contextual biology.
NATURE COMMUNICATIONS
(2021)