Comparative study on the stability of soybean (Glycine max) -conglycinin in vivo

The immunoreactivity and structural variation of -conglycinin in digesta from digestive tracts of pigs were measured by inhibition ELISA and sodium dodecyl sulphate polyacrylamide gel electrophoresis, respectively. Results showed that the immunoreactivity disappearance proportion of -conglycinin significantly increased from stomach to the caecum in all groups (P0.05). In the stomach, upper-jejunum and middle-jejunum, the immunoreactivity disappearance proportion of -conglycinin significantly increased among these three groups (P0.05), while it has no significant difference in ileum and caecum (P0.05). The ', subunits of -conglycinin were easier to digest than the subunit. It indicated that immunoreactivity disappearance proportion of -conglycinin tended to increase with the growth of age and the descending down of digestive tract, while the subunits of -conglycinin are more stable to digestion than ', subunits.