Journal
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
Volume 30, Issue 6, Pages 1012-1019Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/19440049.2013.794307
Keywords
desorption electrospray ionisation; DESI; mass spectrometry; imaging; injection sites; anabolic steroid esters; bovine tissue
Funding
- Dutch Ministry of Economic Affairs, Agriculture and Innovation [72592.01]
- Dutch Science Foundation (NWO)
Ask authors/readers for more resources
In this study, desorption electrospray ionisation (DESI) linear ion trap tandem mass spectrometry (MSn) was applied for the confirmation and three-dimensional profiling of anabolic steroid esters in an injection site of bovine muscle. The spatial resolution of the DESI-MSn was demonstrated by scanning hormone esters and marker ink lines drawn at various distances on a microscopic slide at set distances, using an x-scanner with manual y and z adjustment. Tissue slices of bovine muscle injected with a hormone cocktail were analysed. All anabolic steroid esters could be directly detected in the sample and confirmed on the basis of identification points awarded for selected MS/MS transitions according to the performance criteria given in Commission Decision 2002/657/EC. Moreover, the injection site could be mapped by two-dimensional and three-dimensional imaging MS, showing a horizontal and vertical distribution through the muscle tissue. This DESI approach offers potential for analysis of injection sites of steroid esters from illegally treated animals; moreover, direct analysis by ambient imaging DESI-MS still allows conventional extraction and analysis of the whole tissue for further confirmatory or contra-analysis afterwards.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available