Journal
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
Volume 27, Issue 6, Pages 749-763Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/19440040903503070
Keywords
authenticity; molecular biology; PCR; traceability; animal products; meat; processed foods
Funding
- Ministerio de Educacion y Ciencia of Spain [AGL 2007-60077]
- Comunidad de Madrid (Spain) [S-0505/AGR/000265]
- Ministerio de Ciencia e Innovacion (Spain)
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Species-specific real-time polymerase chain reaction (PCR) assays using TaqMan probes have been developed for verifying the labeling of meat and commercial meat products from game birds, including quail, pheasant, partridge, guinea fowl, pigeon, Eurasian woodcock and song thrush. The method combines the use of species-specific primers and TaqMan probes that amplify small fragments (amplicons 150 base pairs) of the mitochondrial 12S rRNA gene, and an endogenous control primer pair that amplifies a 141-bp fragment of the nuclear 18S rRNA gene from eukaryotic DNA. Analysis of experimental raw and heat-treated binary mixtures as well as of commercial meat products from the target species demonstrated the suitability of the assay for the detection of the target DNAs.
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