High-throughput enzyme-linked immunoabsorbant assay (ELISA) electrochemiluminescent detection of botulinum toxins in foods for food safety and defence purposes

Clostridum species produce seven serotypes (A-G) of botulinum toxin, four of which (A, B, E, and F) are normally associated with human illness. To date, the most reliable test for botulinum toxin is the mouse bioassay. The authors' laboratory has been exploring the use of an antibody-based assay similar to an enzyme-linked immunoabsorbant assay (ELISA) but utilizing electrochemiluminescent technology (BioVerify (R) assay) as an alternative to the mouse bioassay for testing food samples. The detection limit of this assay is as low as 10 ng g(-1) depending on the food matrix and the serotype detected. Detection of botulinum toxin between 10 and 200 ng g(-1) is a linear curve allowing for the possibility of performing quantitative as well as qualitative testing of samples. The ease of the assay, limited sample preparation, and low detection limit make the BioVerify assay and instrument an excellent, high-throughput option for detecting botulinum toxins in food matrices.