Article
Oncology
Yang Li, Zhenxing Wang, Rao Fu, Shuang Wang, Tingting Zhang, Xudong Tian, Dawei Yang
Summary: Detection of CTCs using NE-FISH could be helpful in the diagnosis of EC. The proportion of CTCs with chromosome 7 triploidy was related to distant metastasis and TNM stage. Patients with CTCs ≥ 3 had short OS, while distant metastasis was an independent factor indicating a poor prognosis for patients with EC.
FRONTIERS IN ONCOLOGY
(2022)
Article
Oncology
Ivonne Nel, Erik W. Morawetz, Dimitrij Tschodu, Josef A. Kas, Bahriye Aktas
Summary: This study focused on the mechanical characterization of circulating non-hematopoietic cells from breast cancer patients to evaluate its utility for CTC detection. Results showed that CTCs have significantly different mechanical fingerprints compared to healthy white blood cells, and the mechanical fingerprint may be an appropriate tool for marker-free CTC detection.
Review
Oncology
Kai Luo, Xiangkun Wang, Xudong Zhang, Zhongyuan Liu, Shuai Huang, Renfeng Li
Summary: In the past few decades, there have been developments in tumor diagnosis and treatment theory. However, pancreatic cancer mortality is increasing while the mortality rate of other common tumors is decreasing. Traditional imaging methods and biomarkers lack sensitivity and specificity for early micrometastasis detection. Circulating tumor cells (CTCs) have emerged as a non-invasive and real-time liquid biopsy technique with potential for analyzing tumor characteristics and serving as an independent prognostic factor.
FRONTIERS IN ONCOLOGY
(2022)
Article
Oncology
Katarina Kolostova, Eliska Pospisilova, Rafal Matkowski, Jolanta Szelachowska, Vladimir Bobek
Summary: By using the CliniMACS Prodigy (R) machine and CTC lysates, we successfully generated Mo-DCs and demonstrated their potential to activate NTBC in co-cultures.
CANCER IMMUNOLOGY IMMUNOTHERAPY
(2022)
Article
Oncology
Yuanrui Liu, Rong Zhao, Zaichun Xie, Zhiyu Pang, Shengjie Chen, Qian Xu, Zhanfeng Zhang
Summary: In this study, circulating tumor cells (CTCs) were detected in the peripheral blood of tumor patients, and their numbers and subtypes were found to be related to different tumor diseases and stages. CTCs with mesenchymal phenotypes were closely associated with lymph node or vascular metastasis. CTC detection can aid in early diagnosis of tumor diseases and monitoring of disease progression.
Review
Oncology
Tao Jin, Yao Chen, Qing-Yan Chen, Yang Xiong, Ji-Qiao Yang
Summary: A meta-analysis confirmed that circulating tumor cells (CTCs) were an important component of noninvasive methods for diagnosing breast cancer, with a high sensitivity (0.69), specificity (0.93), and area under the curve (0.81).
FRONTIERS IN ONCOLOGY
(2023)
Article
Oncology
Ying Xu, Xue Ren, Tong Jiang, Shuang Lv, Kuanke Gao, Yunen Liu, Ying Yan
Summary: This study showed a high rate of positive CTC detection in patients with lung cancer, and the number, subtype, and hTERT-positive expression of CTCs were closely related to patients' overall response rate, progression-free survival, and overall survival with radiotherapy. EMCTCs and hTERT-positive expression of CTCs are expected to be important biological indicators for predicting radiotherapy efficacy and the prognosis in patients with lung cancer. These results may be useful in improving disease stratification for future clinical trials and may help in clinical decision-making.
Article
Plant Sciences
Zu-Jun Que, Yun Yang, Hai-Tao Liu, Wen-Ji Shang-Guan, Pan Yu, Li-Hua Zhu, He-Gen Li, Huai-Min Liu, Jian-Hui Tian
Summary: The study aimed to investigate the anti-metastatic mechanism of Jinfukang in lung cancer by intervening in circulating tumor cells (CTCs). Results showed that Jinfukang inhibited the proliferation, migration, and invasion of CTCs, as well as inducing anoikis through suppression of the integrin/Src pathway. This suggests that Jinfukang may exert its anti-metastatic effects in lung cancer through anoikis.
JOURNAL OF ETHNOPHARMACOLOGY
(2021)
Review
Chemistry, Physical
Man Wu, Yuhang Huang, Yang Zhou, Hui Zhao, Yuwei Lan, Zhibin Yu, Chunping Jia, Hui Cong, Jianlong Zhao
Summary: Circulating tumor cells (CTCs) enumeration is widely used for early diagnoses, chemotherapy efficacy evaluation, and cancer prognosis. New types of circulating cancer-related cells have been reported to be correlated to cancer diagnosis, metastasis, or prognosis, and they present challenges to microfluidic devices for CTCs detection. This review focuses on the properties and clinical significance of these novel circulating cancer-related cells, and discusses the challenges they pose to microfluidic chip enrichment.
Article
Oncology
Retno Ningsi, Maha Elazezy, Luisa Stegat, Elena Laakmann, Sven Peine, Sabine Riethdorf, Volkmar Mueller, Klaus Pantel, Simon A. Joosse
Summary: CTCs have been found to be associated with metastasis and survival in breast cancer patients. Evaluating the expression of estrogen receptors (ER) in CTCs may help with personalized treatment. Some patients have both ER-positive and ER-negative CTCs at the time of metastasis, which may lead to resistance to hormonal therapy. Therefore, monitoring the status of CTC-ER in breast cancer patients can provide prognostic and predictive value.
Article
Biochemistry & Molecular Biology
Ines Martinez-Pena, Pablo Hurtado, Nuria Carmona-Ule, Carmen Abuin, Ana Belen Davila-Ibanez, Laura Sanchez, Miguel Abal, Anas Chaachou, Javier Hernandez-Losa, Santiago Ramon Y. Cajal, Rafael Lopez-Lopez, Roberto Pineiro
Summary: The study using zebrafish embryo as a model system revealed the metastatic competency and molecular mechanisms of breast cancer CTCs and CTC-clusters. While CTC-clusters showed lower dissemination and invasion abilities, they demonstrated enhanced survival and proliferation capacities, along with increased resistance to fluid shear stress. These findings were confirmed in mouse studies, emphasizing the importance of understanding the biology of CTCs and CTC-clusters in metastasis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Huan Deng, Xiangqin Liu, Jie Chen, Yi He, Lanke Lin, Xin Liu, Jiang Chen, Xiaoqi Liu
Summary: Efficiently capturing circulating tumor cells (CTCs) in blood is crucial for cancer screening, treatment, and risk assessment of metastasis or recurrence. Current methods for capturing CTCs often require multiple chemical reagents and a complex pre-treatment process. In this study, a simple strategy was developed using photo-functionalized titanium dioxide (TiO2) to immobilize EpCAM antibodies without additional chemical reagents. The positive charge of the TiO2 surface allowed for electrostatic interaction and effective immobilization of EpCAM antibodies, leading to efficient CTC capture both in vitro and in vivo. This strategy shows promise for future applications in CTC capture.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Zachery R. Reichert, Tadas Kasputis, Srinivas Nallandhighal, Sophia M. Abusamra, Amy Kasputis, Saloni Haruray, Yugang Wang, Shamara Williams, Udit Singhal, Ajjai Alva, Frank C. Cackowski, Megan E. V. Caram, Phillip L. Palmbos, Sarah E. Yentz, David C. Smith, Joshi J. Alumkal, Todd M. Morgan
Summary: The biological heterogeneity of metastatic prostate cancer affects personalized therapeutic approaches. A liquid biopsy approach can identify blood-based prognostic biomarkers for predicting disease progression and treatment response in patients with metastatic hormone-sensitive prostate cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Oncology
Beatrice Aramini, Valentina Masciale, Chiara Arienti, Massimo Dominici, Franco Stella, Giovanni Martinelli, Francesco Fabbri
Summary: The roles and connections of small molecules such as cancer stem cells, circulating tumor cells, and cancer-associated fibroblasts in solid tumors have been under-debated but of significant interest in recent decades. These molecules may play a role in tumor recurrence, drug resistance, and treatment response, but their mechanisms and molecular pathways are still unknown. Defining new molecules to fight against cancer is of great interest to the scientific community. Cancer stem cells, circulating tumor cells, and cancer-associated fibroblasts play important roles in the tumor microenvironment, and discovering biomarkers related to these cell populations and defining the network among them and the tumor microenvironment are becoming increasingly important.
Article
Biophysics
Syed Harris Hussain, Cesar S. Huertas, Arnan Mitchell, Anne-Laure Deman, Emmanuelle Laurenceau
Summary: Analysis of circulating tumor cells (CTCs) and their associated biomarkers can provide valuable information for lung and prostate cancer, aiding in early detection and personalized treatment selection. Biosensors offer an elegant alternative to conventional methods for real-time, multiplexed analysis of cancer biomarkers at lower concentrations.
BIOSENSORS & BIOELECTRONICS
(2022)
Letter
Immunology
Jan Havlin, Aneta Skotnicova, Eliska Dvorackova, Petr Hubacek, Monika Svorcova, Jan Lastovicka, Anna Sediva, Tomas Kalina, Robert Lischke
Editorial Material
Obstetrics & Gynecology
Patrycja Stec, Anna Rygula, Marcin Jedryka, Rafal Matkowski, Piotr Lepka
Summary: This paper presents a case of a 36-year-old patient initially diagnosed with extensive endometriosis involving the anterior abdominal wall and the pelvis minor. After biopsy, a diagnosis of advanced low-grade serous ovarian cancer was established. The diagnostic methods used and the extent of surgery with reconstruction of the anterior abdominal wall were described.
GINEKOLOGIA POLSKA
(2023)
Article
Oncology
Andrej Ozaniak, Jitka Smetanova, Robin Bartolini, Michal Rataj, Linda Capkova, Jaromir Hacek, Martina Fialova, Lenka Krupickova, Ilja Striz, Robert Lischke, Jirina Bartunkova, Zuzana Strizova
Summary: Combination strategies targeting both tumor-infiltrating lymphocytes and macrophages may represent a relevant treatment approach for metastatic or recurrent soft tissue sarcomas (STSs), as macrophages dominate the immune landscape of STSs.
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
(2023)
Review
Pharmacology & Pharmacy
Capucine Casari, Rene Novysedlak, Jiri Vachtenheim, Robert Lischke, Zuzana Strizova
Summary: A systematic review was conducted to evaluate the potential of immune checkpoint inhibitors (ICIs) combined with chemotherapy, radiotherapy, and/or biologic therapy in metastatic esophageal carcinoma (EC) patients. The review discussed the effects of different treatment approaches on the tumor microenvironment and reviewed the current clinical trials to provide a rationale for immunotherapy application in combination settings. PD-L1 expression was found to be higher in squamous cell carcinoma (SCC) compared to adenocarcinoma, suggesting that ICIs plus chemotherapy may be a feasible option for SCC patients. Radiation was shown to induce immune checkpoint molecules and activate cytotoxic T cells, providing a rationale for ICI administration in combination with radiotherapy. The combination of ICIs with biologic therapy was deemed safe, but its impact on clinical outcomes varied.
Article
Environmental Sciences
Oliver Czech, Katarzyna Siewierska, Aleksandra Krzywinska, Jakub Skorniak, Adam Maciejczyk, Rafal Matkowski, Joanna Szczepanska-Gieracha, Iwona Malicka
Summary: This study aimed to evaluate the effectiveness of VR therapy on improving the mental state, quality of sleep, and physical activity of breast cancer patients. The results showed that VR therapy can significantly reduce the severity of depressive symptoms, decrease destructive coping styles, and have a positive impact on constructive coping styles.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2023)
Article
Multidisciplinary Sciences
Anna Urbaniak, Karolina Jablonska, Jaroslaw Suchanski, Aleksandra Partynska, Katarzyna Szymczak-Kulus, Rafal Matkowski, Adam Maciejczyk, Maciej Ugorski, Piotr Dziegiel
Summary: This study demonstrates that high expression of prolactin-induced protein (PIP) sensitizes breast cancer (BC) cells to anti-cancer drugs. Increased sensitivity to chemotherapy is the result of pro-apoptotic activity of PIP, which is evidenced by up-regulation of specific pro-apoptotic genes. The findings suggest that PIP can serve as a marker for the prognostic evaluation of adjuvant chemotherapy.
SCIENTIFIC REPORTS
(2023)
Article
Respiratory System
Jaromir Vajter, Jiri Vachtenheim, Zuzana Prikrylova, Jan Berousek, Tomas Vymazal, Robert Lischke, Archer Kilbourne Martin, Miroslav Durila
Summary: After lung transplantation, primary graft dysfunction (PGD) contributes to early postoperative morbidity. Intraoperative transfusion of blood products and ischemia-reperfusion injury play a role in PGD development. A randomized clinical trial showed that targeted coagulopathy management and intraoperative administration of 5% albumin can reduce blood loss during lung transplantation surgery. The analysis further revealed that the POC group had better graft function and lower dosage of norepinephrine compared to the non-POC group. The difference in 1-year survival was not significant.
BMC PULMONARY MEDICINE
(2023)
Article
Immunology
Rosalind Graham, Patrycja Gazinska, Birong Zhang, Atousa Khiabany, Shubhankar Sinha, Thanussuyah Alaguthurai, Fabian Flores-Borja, Jose Vicencio, Fabienne Beuron, Ioannis Roxanis, Rafal Matkowski, Revadee Liam-Or, Andrew Tutt, Tony Ng, Khuloud T. Al-Jamal, You Zhou, Sheeba Irshad
Summary: This study investigates the differential effects of breast cancer subtypes on T cell mediated immune responses through the isolation of EVs from patient serum and cell-line derived EVs. The results show that TNBC patients have the lowest number of EVs in their serum, while ER+HER2+ patients have the highest. Furthermore, EVs derived from different breast cancer subtypes have different immunomodulatory capabilities, with TNBC EVs demonstrating the most immune-suppressive phenotype.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Justyna Wadzynska, Aleksandra Simiczyjew, Katarzyna Pietraszek-Gremplewicz, Magdalena Kot, Marcin Zietek, Rafal Matkowski, Dorota Nowak
Summary: The microenvironment of melanoma, consisting of cellular elements and physicochemical conditions, plays a significant role in melanoma progression, proliferation, metabolism, and response to anti-cancer therapy. Fibroblasts, adipocytes, and keratinocytes present in the tumor niche influenced melanoma cells' signaling pathways, metabolism, and proliferation. The findings indicate the efficacy of the proposed combination therapy for melanoma remains even when cancer-associated cells are present in the vicinity.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2023)
Article
Oncology
Bartosz Dolega-Kozierowski, Piotr Kasprzak, Michal Lis, Bartlomiej Szynglarewicz, Rafal Matkowski, Marek Sawicki, Mateusz Dymek, Adrianna Szumiejko, Gustavo Carmo, Artur Kwiatkowski, Daniel Grzegorz Solinski, Mariusz Ptak
Summary: This study presents a new approach for the pre-operative assessment and surgical planning of breast cancer patients, utilizing a comprehensive range of techniques to improve the accuracy and efficacy of surgical procedures.
BREAST CANCER RESEARCH AND TREATMENT
(2023)
Article
Oncology
Yongchao Dou, Lizabeth Katsnelson, Marina A. Gritsenko, Yingwei Hu, Boris Reva, Runyu Hong, Yi-Ting Wang, Iga Kolodziejczak, Rita Jui-Hsien Lu, Chia-Feng Tsai, Wen Bu, Wenke Liu, Xiaofang Guo, Eunkyung An, Rebecca C. Arend, Jasmin Bavarva, Lijun Chen, Rosalie K. Chu, Andrzej Czekanski, Teresa Davoli, Elizabeth G. Demicco, Deborah Delair, Kelly Devereaux, Saravana M. Dhanasekaran, Peter Dottino, Bailee Dover, Thomas L. Fillmore, McKenzie Foxall, Catherine E. Hermann, Tara Hiltke, Galen Hostetter, Marcin Jedryka, Scott D. Jewell, Isabelle Johnson, Andrea G. Kahn, Amy T. Ku, Chandan Kumar-Sinha, Pawel Kurzawa, Alexander J. Lazar, Rossana Lazcano, Jonathan T. Lei, Yi Li, Yuxing Liao, Tung-Shing M. Lih, Tai-Tu Lin, John A. Martignetti, Ramya P. Masand, Rafal Matkowski, Wilson Mckerrow, Mehdi Mesri, Matthew E. Monroe, Jamie Moon, Ronald J. Moore, Michael D. Nestor, Chelsea Newton, Tatiana Omelchenko, Gilbert S. Omenn, Samuel H. Payne, Vladislav A. Petyuk, Ana I. Robles, Henry Rodriguez, Kelly V. Ruggles, Dmitry Rykunov, Sara R. Savage, Athena A. Schepmoes, Tujin Shi, Zhiao Shi, Jimin Tan, Mason Taylor, Mathangi Thiagarajan, Joshua M. Wang, Karl K. Weitz, C. M. Williams, Yige Wu, Matthew A. Wyczalkowski, Xu Zhang, Rui Zhao, David Mutch, Arul M. Chinnaiyan, Richard D. Smith, Alexey I. Nesvizhskii, Pei Wang, Maciej Wiznerowicz, Li Ding, D. R. Mani, Hui Zhang, Matthew L. Anderson, Karin D. Rodland, Bing Zharg, Tao Liu, David Fenyo
Summary: This study characterizes a cohort of endometrial carcinoma patients using multiple omics platforms, identifying potential markers and mutations related to treatment response. The findings suggest strategies for more precise treatment based on these markers and mutations.
Article
Allergy
Marcin Zietek, Jedrzej Wierzbicki, Maciej Nowacki, Rafal Czajkowski, Piotr Wronski, Katarzyna Nowacka, Edyta Pawlak, Adam Maciejczyk, Rafal Matkowski
Summary: Among melanoma patients, it is important to pay attention not only to early detection of melanoma recurrence and progression, but also the occurrence of new primary foci or independent skin cancers.
POSTEPY DERMATOLOGII I ALERGOLOGII
(2023)
Article
Pharmacology & Pharmacy
Giulia Toffanin, Andrej Ozaniak, Robin Bartolini, Martin Komarc, Rene Novysedlak, Michal Rataj, Jitka Smetanova, Antonio Rosato, Robert Lischke, Jirina Bartunkova, Zuzana Strizova
Summary: This study aimed to investigate the effects of Oncostatin M (OSM) on immune cells derived from liposarcoma, leiomyosarcoma, and myxofibrosarcoma, and the potential cooperation between OSM and nivolumab in treating these soft tissue sarcomas (STSs). The results suggest that OSM may have a role in the treatment of leiomyosarcoma, myxofibrosarcoma, and liposarcoma. However, further histotype-tailored studies are needed to fully comprehend the functions of OSM in STSs.
Article
Immunology
Aleksandra Grzegorczyk, Zuzanna Marczynska, Rafal Matkowski, Marcin Zietek, Wojciech Pietras, Elzbieta Latos-Grazynska, Krzysztof Kalwak, Iwona Dachowska-Kalwak
Summary: This study presents a case of a 17-year-old female patient who had previously undergone surgical resection of melanoma and was administered PD-1 inhibitor nivolumab as therapy. The patient experienced several complications during treatment but follow-up showed no evidence of metastasis or lymph node enlargement.
CENTRAL EUROPEAN JOURNAL OF IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Aleksandra Simiczyjew, Justyna Wadzynska, Katarzyna Pietraszek-Gremplewicz, Magdalena Kot, Marcin Zietek, Rafal Matkowski, Dorota Nowak
Summary: Melanoma cells have an impact on adipocytes in terms of their characteristics and metabolic processes, leading to reduced lipid content and increased lactate secretion. The changes in adipocytes following coculture enhance the proliferation of melanoma cells.
CELLULAR & MOLECULAR BIOLOGY LETTERS
(2023)
