Article
Biochemistry & Molecular Biology
Sadra Hesami, Shahabeddin Safi, Kambiz Larijani, Hassanali Naghdi Badi, Vahid Abdossi, Milad Hadidi
Summary: Essential oils from greater celandine roots and leaves were extracted and loaded into chitosan nanoparticles, with good encapsulation efficiency confirmed. These nanoparticles showed a dose-dependent effect on MCF-7 cell line, with CNPs-GCLEO displaying the highest rate of apoptosis. Encapsulation of GCREO and GCLEO in CNPs proved to be an efficient technique for enhancing their anti-cancer activity against MCF-7 cell line.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Genetics & Heredity
Ali Parsania, Mohammad Hassan Pouriayevali, Masoud Parsania, Mahdi Ghorbani
Summary: The study showed that the crude alkaloid extract of Chelidonium majus induces apoptosis in HeLa cells through the DR4 and DR5 death receptors, making it a potential natural product candidate for cervical carcinoma chemotherapy. Further research is needed to identify the essential components of the alkaloid extract that trigger apoptosis.
Article
Pharmacology & Pharmacy
Alicja Warowicka, Badr Qasem, Anna Dera-Szymanowska, Maria Wolun-Cholewa, Patryk Florczak, Nikodem Horst, Marta Napierala, Krzysztof Szymanowski, Lukasz Popenda, Grazyna Bartkowiak, Ewa Florek, Anna Gozdzicka-Jozefiak, Piotr Mlynarz
Summary: The study found that protoberberine compounds from Chelidonium majus effectively prevent the recurrence of endometriosis in rats. Metabolomics analysis revealed significant changes in metabolite concentrations related to energy homeostasis after treatment. This suggests that natural protoberberine plant compounds have pharmaceutical activity and further research should focus on understanding their mechanisms of action.
Article
Biotechnology & Applied Microbiology
Lei Shen, Soon Lee, Jong Cheon Joo, Eunmi Hong, Zhen Yang Cui, Eunbi Jo, Soo Jung Park, Hyun-Jin Jang
Summary: Chelidonium majus induces apoptosis in ovarian cancer cells by regulating the expression levels of ATF3, Tip60, and Foxo3a. This provides evidence for the potential therapeutic role of Chelidonium majus in ovarian cancer.
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Medicinal
Nilo E. Gardin, Anne Jacqueline Braga
Summary: A study on the use of greater celandine in treating COVID-19 outpatients revealed that most patients experienced complete or near complete clinical improvement within 1-9 days of treatment, with symptoms such as fever, fatigue, cough, sore throat, coryza, anosmia, ageusia, and headache. This small case series may inspire further research on Chelidonium majus as a potential therapeutic option for COVID-19.
PHYTOTHERAPY RESEARCH
(2021)
Article
Chemistry, Physical
Sylwia Zielinska, Adam Matkowski, Karolina Dydak, Monika Ewa Czerwinska, Magdalena Dziagwa-Becker, Mariusz Kucharski, Magdalena Wojciak, Ireneusz Sowa, Stanislawa Plinska, Karol Fijalkowski, Daria Ciecholewska-Jusko, Michal Broda, Damian Gorczyca, Adam Junka
Summary: In this study, a bi-functional bacterial-nano-cellulose (BNC) carrier system was developed for cell cultures of Chelidonium majus, a medicinal plant producing antimicrobial compounds. The unpurified BNC synthesized for 3 days exhibited the highest content and most complex composition of pharmacologically active substances, showing anti-inflammatory and antimicrobial activities. This modified composite material has potential applications in combating microbial pathogens.
Article
Environmental Sciences
Ana Popovic, Milena Deljanin, Suzana Popovic, Danijela Todorovic, Predrag Djurdjevic, Sanja Matic, Milan Stankovic, Dusko Avramovic, Dejan Baskic
Summary: The study showed that ethanolic extract of Chelidonium majus was able to activate subpopulations of peripheral blood mononuclear cells (PBMNCs), enhance cytotoxic activity, and promote proliferation of immune cells.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL HEALTH RESEARCH
(2022)
Article
Nutrition & Dietetics
Ana Rocio Rivera-Martinez, Itzen Aguiniga-Sanchez, Jorge Cadena-Iniguez, Isabel Soto-Cruz, Alberto Monroy-Garcia, Guadalupe Gomez-Garcia, Edgar Ledesma-Martinez, Benny Weiss-Steider, Edelmiro Santiago-Osorio
Summary: The wild species Sechium chinantlense (SCH) has been found to have antitumor properties, inhibiting proliferation in cervical cancer cells without harming normal cells.
Review
Biotechnology & Applied Microbiology
Shahin Amiri, Setare Adibzadeh, Samaneh Ghanbari, Behnaz Rahmani, Mohammad H. Kheirandish, Aref Farokhi-Fard, Mansoureh S. Dastjerdeh, Fatemeh Davami
Summary: Chinese hamster ovary (CHO) cells are the most commonly used host cell system for industrial production of recombinant protein biopharmaceuticals. The use of CRISPR-Cas technology along with donor design strategies has allowed scientists to efficiently modify CHO cell line genomes and achieve consistent and high-quality protein expression. This review summarizes the recent advancements in CHO cell line development, including different cell engineering approaches and targeted integration strategies, which can improve the product development process and yield.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Article
Food Science & Technology
Shivraj Hariram Nile, Hui Wang, Arti Nile, Xianmin Lin, Huilin Dong, Baskar Venkidasamy, Elwira Sieniawska, Gansukh Enkhtaivan, Guoyin Kai
Summary: Metabolic variations, antioxidant potential and cytotoxic effects were investigated in different parts of C. majus L. using spectroscopic and chromatographic methods. The results revealed that the flower extract showed higher antioxidant activity, while the pod and leaf extracts exhibited higher cytotoxic effects in the in vitro SRB assay. These findings suggest promising potential for the development of high value products for phytomedicine applications.
FOOD AND CHEMICAL TOXICOLOGY
(2021)
Article
Plant Sciences
Valerija Krizhanovska, Inga Sile, Arta Kronberga, Ilva Nakurte, Ieva Mezaka, Maija Dambrova, Osvalds Pugovics, Solveiga Grinberga
Summary: The study found significant differences in the phytochemical composition and cytotoxic activity of Chelidonium majus extracts between wild-grown and organically cultivated specimens. Cultivation resulted in a significant increase in alkaloid content and enhanced cytotoxicity of the extracts.
Article
Chemistry, Multidisciplinary
Abhinav Prasad, Ashapurna Khatua, Yugal Kishore Mohanta, Muthupandian Saravanan, Ramovatar Meena, Ilora Ghosh
Summary: This study demonstrates the effectiveness of a combination therapy using low-dose gold nanoparticles and glutamine deprivation in inhibiting the growth of human cervical cancer cells. The combination treatment resulted in significant inhibition of cell growth and induced cellular uptake of nanoparticles. The therapy also induced oxidative stress conditions and mitochondrial inactivity in the cancer cells.
Article
Plant Sciences
Ramaraj Sathasivam, Hyeon Ji Yeo, Chang Ha Park, Minsol Choi, Haejin Kwon, Ji Eun Sim, Sang Un Park, Jae Kwang Kim
Summary: This study identified and analyzed the carotenoid biosynthesis pathway genes of Chelidonium majus. The genes showed tissue-specific expression, with the highest levels in flowers. The study provides insights for optimizing carotenoid production in C. majus and lays a foundation for further molecular genetics and functional analysis studies.
Article
Biochemistry & Molecular Biology
Oskar Musidlak, Alicja Warowicka, Justyna Broniarczyk, Damian Adamczyk, Anna Gozdzicka-Jozefiak, Robert Nawrot
Summary: Latex of Chelidonium majus L. has antiviral and antitumor properties, with alkaloids and proteins playing important roles in its pharmacological activities.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Maciej Strzemski, Slawomir Dresler, Beata Podkoscielna, Kamil Skic, Ireneusz Sowa, Daniel Zaluski, Rob Verpoorte, Sylwia Zielinska, Pawel Krawczyk, Magdalena Wojciak
Summary: This study evaluated the potential of using volatile natural deep eutectic solvents (VNADESs) for the efficient and environmentally friendly extraction of Chelidonium alkaloids. The results showed that the use of appropriate VNADESs formulations led to higher extraction yields compared to traditional methods.
Article
Biochemistry & Molecular Biology
Robert Nawrot, Damian Jozefiak, Anna Sip, Dorota Kuzma, Oskar Musidlak, Anna Gozdzicka-Jozefiak
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2017)
Article
Oncology
Wojciech Kwasniewski, Maria Wolun-Cholewa, Jan Kotarski, Wojciech Warchol, Dorota Kuzma, Anna Kwasniewska, Anna Gozdzicka-Jozefiak
Article
Plant Sciences
Alicja Warowicka, Lukasz Popenda, Grazyna Bartkowiak, Oskar Musidlak, Litowczenko-Cybulska Jagoda, Dorota Kuzma, Robert Nawrot, Stefan Jurga, Anna Gozdzicka-Jozefiak
Article
Materials Science, Biomaterials
Edyta Hebda, Artur Bukowczan, Slawomir Michalowski, Sebastian Wronski, Paulina Urbaniak, Mariusz Kaczmarek, Emilia Hutnik, Aleksandra Romaniuk, Maria Wolun-Cholewa, Krzysztof Pielichowski
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2020)
Review
Virology
Alicja Warowicka, Robert Nawrot, Anna Gozdzicka-Jozefiak
ARCHIVES OF VIROLOGY
(2020)
Article
Biochemistry & Molecular Biology
Robert Nawrot, Oskar Musidlak, Jakub Barylski, Grzegorz Nowicki, Sophia Baldysz, Anna Czerwoniec, Anna Gozdzicka-Jozefiak
Summary: TLPs are a protein family with significant sequence homology to thaumatin, but not sweet-tasting. A new TLP protein, CcTLP1, was identified in tubers of Corydalis cava, with potentially defensive functions against various stress conditions. CcTLP1 showed highest expression levels in plant stems, suggesting a role in plant growth and development.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Robert Nawrot, Alicja Warowicka, Piotr Jozef Rudzki, Oskar Musidlak, Katarzyna Magdalena Dolata, Jacek Musijowski, Elzbieta Urszula Stolarczyk, Anna Gozdzicka-Jozefiak
Summary: The study on the interaction between latex protein and alkaloids in Chelidonium majus L. revealed their ability to reduce the viability of cervical cancer cells. This novel approach provides insights into plant defense mechanisms and can contribute to pharmacological research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Joanna Gracz-Bernaciak, Oliwia Mazur, Robert Nawrot
Summary: Latex, produced by specialized cells, plays a crucial role in plants' defense system against herbivory and pathogens, containing a variety of active compounds beneficial for both plants and human health. Despite the long-standing tradition of using latex in medicine, there are still many promising molecules waiting to be explored.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Karolina Iwona Kulinska, Miroslaw Andrusiewicz, Anna Dera-Szymanowska, Maria Billert, Marek Skrzypski, Krzysztof Szymanowski, Ewa Nowak-Markwitz, Malgorzata Kotwicka, Maria Wolun-Cholewa
Summary: The study found that patients with endometriosis have reduced PNX levels, increased LH/FSH ratio, and elevated 17β-estradiol concentration. GPR173 expression was lower in ectopic endometrium. These findings provide new insights into the pathophysiology and diagnosis and treatment strategies for endometriosis.
Article
Biochemistry & Molecular Biology
Oskar Musidlak, Alicja Warowicka, Justyna Broniarczyk, Damian Adamczyk, Anna Gozdzicka-Jozefiak, Robert Nawrot
Summary: Latex of Chelidonium majus L. has antiviral and antitumor properties, with alkaloids and proteins playing important roles in its pharmacological activities.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Tomasz Geca, Aleksandra Stupak, Robert Nawrot, Anna Gozdzicka-Jozefiak, Anna Kwasniewska, Wojciech Kwasniewski
Summary: Fetal growth restriction (FGR) is a condition where the fetus does not reach its full potential for growth and can lead to obstetric and neonatal complications. This study compared the placental proteome of normal pregnancies and pregnancies complicated by FGR, revealing significant differences between the two groups. Further research is needed to better understand maternal and fetal adaptations to FGR.
MOLECULAR MEDICINE REPORTS
(2022)
Review
Plant Sciences
Oliwia Mazur, Sophia Baldysz, Alicja Warowicka, Robert Nawrot
Summary: Latex-bearing plants have been extensively studied for their anticancer activity, with numerous in vitro and in vivo studies conducted. However, clinical studies are still limited. This review summarizes the current knowledge on the cytotoxicity and anticancer activities of latex-bearing plant extracts, and highlights the potential for novel cancer therapies.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Aleksandra Stupak, Tomasz Geca, Anna Kwasniewska, Radoslaw Mlak, Pawel Piwowarczyk, Robert Nawrot, Anna Gozdzicka-Jozefiak, Wojciech Kwasniewski
Summary: A comparative analysis was conducted to assess the impact of bacteria on placental development and function in pregnancies with late fetal growth restriction (FGR). The presence of microorganisms in various components of the pregnancy disproves the theory of the sterile uterus. Bacterial infections have been linked to maternal overproduction of pro-inflammatory cytokines and can cause both short- and long-term problems.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Robert Nawrot
CURRENT PROTEIN & PEPTIDE SCIENCE
(2017)