Article
Plant Sciences
Nora Gampe, Zoltan Szakacs, Andras Darcsi, Imre Boldizsar, Eva Szoke, Inna Kuzovkina, Laszlo Kursinszki, Szabolcs Beni
Summary: Hairy root cultures of Ononis spinosa and Ononis arvensis were established and analyzed for their isoflavonoid profiles, showing significant differences from wild-grown samples with the most abundant compounds being medicarpin glucoside and sativanone glucoside. The overall isoflavonoid production of the cultures was comparable to wild-grown O. arvensis and approximately twice as high as in wild-grown O. spinosa samples.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Integrative & Complementary Medicine
Manal Ahmad Abbas, Yasser Ibrahim Kandil, Manal Mohammad Abbas
Summary: The study demonstrated that the extract from O. spinosa protected gastric mucosa from ethanol-induced injury by inducing COX-2 expression and reducing oxidative stress in the stomach.
JOURNAL OF TRADITIONAL CHINESE MEDICINE
(2021)
Article
Plant Sciences
Musa H. Abu Zarga, Hala I. Al-Jaber, Mahmoud A. Al-Qudah, Amal M. F. Al-Aboudi
Summary: A new cyclic polyketide compound was isolated from Ononis spinosa in Jordan, showing high antioxidant activity. Puerol B and specionin exhibited the highest radical scavenging power among the compounds tested.
JOURNAL OF ASIAN NATURAL PRODUCTS RESEARCH
(2022)
Review
Biochemistry & Molecular Biology
Aditya Kaul, Walker D. Short, Xinyi Wang, Sundeep G. Keswani
Summary: This article discusses the important role of hyaluronidases in human tissues, especially in promoting disease progression, and the potential benefits as clinical treatments currently and in the future.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Michelle Coleman, Blair Armistead, Austyn Orvis, Phoenicia Quach, Alyssa Brokaw, Claire Gendrin, Kavita Sharma, Jason Ogle, Sean Merillat, Matthew Dacanay, Tsung-Yen Wu, Jeff Munson, Audrey Baldessari, Jay Vornhagen, Anna Furuta, Shayla Nguyen, Kristina M. Adams Waldorf, Lakshmi Rajagopal
Summary: Invasive bacterial infections during pregnancy, such as Group B streptococci (GBS), can lead to preterm birth, stillbirth, and fetal injury. Using a nonhuman primate model, researchers found that GBS expressing hyaluronidase can cause microbial invasion into the amniotic cavity and preterm labor, shedding light on virulence factors and potential therapeutic strategies. Understanding how bacteria evade host defenses during pregnancy is crucial for developing preventive therapies against adverse outcomes.
Article
Surgery
Christine Ryu, Jonathan E. Lu, Sandy Zhang-Nunes
Summary: This study evaluated the in vitro responses of 12 HA fillers to varying doses of recombinant human hyaluronidase (RHH). Results showed different levels of resistance of different fillers to hyaluronidase.
JOURNAL OF PLASTIC RECONSTRUCTIVE AND AESTHETIC SURGERY
(2021)
Article
Neurosciences
Luis F. Queme, Adam J. Dourson, Megan C. Hofmann, Ally Butterfield, Rudolph D. Paladini, Michael P. Jankowski
Summary: This study found that PEGPH20 may sensitize muscle chemosensory nerve afferents by disrupting hyaluronic acid in the muscle extracellular matrix, leading to changes in pain behaviors. Macrophages play an important role in the recovery process after HA disruption.
Article
Clinical Neurology
Sahar Majdi Jaffal, Belal Omar Al-Najjar, Manal Ahmad Abbas
Summary: Ononis Spinosa alleviates CAPS-induced mechanical allodynia through two mechanisms: direct modulation of TRPV1 and involvement of β-adrenoreceptor signaling.
KOREAN JOURNAL OF PAIN
(2021)
Article
Biochemical Research Methods
Nora Gampe, Erzsebet Nagy, Laszlo Kursinszki, Szabolcs Beni
Summary: Quantitative determination of isoflavones, isoflavanones and pterocarpans was carried out using ultra-performance liquid chromatography coupled with ultraviolet diode-array detection. An optimised sample preparation method was developed to convert malonyl glucosides to their glucosidic forms. Chromatographic methods were established for the baseline separation of the compounds in Ononis spinosa and O. arvensis, with no characteristic change observed between the two species in terms of their isoflavonoid pattern.
PHYTOCHEMICAL ANALYSIS
(2021)
Article
Biotechnology & Applied Microbiology
Bo Pang, Jing He, Weijiao Zhang, Hao Huang, Yang Wang, Miao Wang, Guocheng Du, Zhen Kang
Summary: In this study, the active expression of recombinant hPH20 was achieved using the Pichia pastoris expression system, and a fusion mutant with the highest expression level was constructed. The results provide important theoretical guidance for the directed evolution of the enzyme to prepare o-HAs with specific molecular weights.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Maria Jose Gomez-Torres, Paula Saez-Espinosa, Paula Manzano-Santiago, Laura Robles-Gomez, Natalia Huerta-Retamal, Jon Aizpurua
Summary: This study characterized the distribution of SPAM1 in different physiological conditions of human sperm and found that the presence of SPAM1 throughout the sperm head is necessary for proper contact with the cumulus-oocyte matrix.
Article
Pharmacology & Pharmacy
Stephen P. Knowles, Marie A. Printz, David W. Kang, Michael J. LaBarre, Renee P. Tannenbaum
Summary: The study shows that rHuPH20 acts locally when used subcutaneously, with good safety and immunogenicity, suitable for various co-administration situations and exhibiting good tolerability in different patient populations. Subcutaneous administration may play a more significant role in future drug development.
EXPERT OPINION ON DRUG DELIVERY
(2021)
Article
Pharmacology & Pharmacy
Marie A. Printz, Barry J. Sugarman, Rudolph D. Paladini, Michael C. Jorge, Yan Wang, David W. Kang, Daniel C. Maneval, Michael J. LaBarre
Summary: This study evaluated the immunogenicity risk of rHuPH20 by analyzing tissue expression, predicted epitopes, and clinical immunogenicity profiles. The results suggest a weak immunogenic potential of rHuPH20.
Article
Biotechnology & Applied Microbiology
Nafiseh Sanei Ata-Abadi, Mahboobeh Forouzanfar, Kianoush Dormiani, Shiva Rouhollahi Varnosfaderani, Leila Pirjamali, Mohammad Hossein Nasr-Esfahani, Reza Moradi Hajidavaloo
Summary: In this study, we successfully constructed stable cell lines in HEK293T cells using the PhiC31 integrase system, which can efficiently produce and secrete active rhPH20. Quantitative and functional analysis showed that the secreted rhPH20 had specific activity and could remove cumulus cells from oocytes. Additionally, the results demonstrated the ability of the PhiC31 enzyme to insert the PH20-expressing donors into specific pseudo attP sites in the genome of target cells with different copy numbers.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Plant Sciences
Motahareh Khojasteh Rad, Askar Ghani, Esmaeel Ghani
Summary: This study evaluated the effects of Capparis spinosa leaf extract on human sperm function, showing improvements in motility and viability while reducing DNA fragmentation. The findings suggest the potential positive effects of C. spinosa leaf extract on male infertility treatment.
JOURNAL OF ETHNOPHARMACOLOGY
(2021)
Article
Multidisciplinary Sciences
Nora Gampe, Dominika Noemi David, Krisztina Takacs-Novak, Anders Backlund, Szabolcs Beni
Summary: Isoflavonoids from Ononis species were found to have optimal polarity for BBB permeation and showed a high potential for MAO-B inhibition.
Article
Biochemical Research Methods
Nora Gampe, Lilla Ladocsi, Ida Fejos, Imre Boldizsar, Andras Darcsi, Szabolcs Beni
Summary: Non-protein amino acids, important metabolites of the Fabaceae family, have valuable biological effects. A chiral separation method was developed for homoproline and homopipecolic acid enantiomers and applied to plant sample analysis. The presence of these amino acids was confirmed in Fabaceae species, with significant variations observed between species, geographical origins, and botanical parts.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Biochemical Research Methods
Orsolya Takacs, Andrea Nagyne Nedves, Imre Boldizsar, Maria Hohn, Szabolcs Beni, Nora Gampe
Summary: This study investigated the 3-NPA content of several plants and developed a validated quantitative method to evaluate 3-NPA concentrations in different herbal samples and cosmetic products. New 3-NPA-containing species were identified using highly sensitive analytical methods, and the developed method proved suitable for determining 3-NPA concentrations in herbal samples.
PHYTOCHEMICAL ANALYSIS
(2022)
Article
Chemistry, Medicinal
Laura Goracci, Alessandra Nurisso, Emile Roussel, Basile Peres, Vincent Chaptal, Pierre Falson, Christelle Marminon, Joachim Jose, Marc Le Borgne, Ahcene Boumendjel
Summary: Human breast cancer resistance protein (BCRP), also known as ABCG2, plays a crucial role in tumor cells' resistance to multiple drugs. In this study, the researchers used computer-aided drug design (CADD) to guide the design of effective ABCG2 inhibitors. The FLAPpharm method was employed to understand the pharmacological features of one of the ABCG2 binding sites involved in regulating the transporter's ATPase activity. The model was evaluated using three predicted compounds, which showed high inhibitory activity against BCRP in the nanomolar range for the best compound.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Cecile Gasse, Puneet Srivastava, Guy Schepers, Joachim Jose, Marcel Hollenstein, Philippe Marliere, Piet Herdewijn
Summary: Chemical cell surface modification is an important field with great potential in tissue engineering, cell-based immunotherapy, and regenerative medicine. However, research on engineering bacterial tissues through chemical cell surface modification is lacking, especially in finding suitable molecular handles. In this study, a novel strategy using orthogonal nucleic acid-protein conjugation was developed to induce artificial bacterial aggregation. This system combines the selective and stable linkage of a protein Tag at the cell surface with the modularity and reversibility of aggregation through oligonucleotide hybridization. The immobilization of XNA via covalent, SNAP-tag-mediated interactions on cell surfaces for bacterial aggregation is reported for the first time.
Article
Chemistry, Medicinal
Magdalena N. Wojciechowski, Sebastian Schreiber, Joachim Jose
Summary: This study presents a ligand-binding approach using surface-displayed HCN4 C-Linker-CNBD on E. coli to monitor the binding of 8-Fluo-cAMP. The binding affinity and mode were determined, and a new HCN4 channel-targeting ligand was identified.
Article
Biophysics
Jaeyong Jung, Ji-Hong Bong, Jeong Soo Sung, Jun-Hee Park, Tae-Hun Kim, Soonil Kwon, Min-Jung Kang, Joachim Jose, Jae-Chul Pyun
Summary: The detection of SARS-CoV-1 was achieved using SPR biosensor and impedance spectrometry with screened Fv-antibodies. Two Fv-variants (clones) with specific affinity to SARS-CoV-1 were identified and named Anti-SP1 and Anti-SP2. The binding affinities of these Fv-variants were analyzed and their binding constants (KD) were estimated. The expressed Fv-antibodies, including fusion proteins with GFP, were also tested for their binding affinities to the SP. The feasibility of detecting SARS-CoV-1 using immobilized Fv-antibodies was demonstrated.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Biochemistry & Molecular Biology
Sarah Hardebeck, Sebastian Schreiber, Annika Adick, Klaus Langer, Joachim Jose
Summary: Proliferating cell nuclear antigen (PCNA) is a key regulator of human DNA metabolism and interacts with p15, playing a role in DNA replication and repair. A FRET-based assay was developed to analyze the PCNA-p15 interaction, which can be used for drug screening and studying mutation-induced changes in affinity. This assay has demonstrated its versatility in analyzing disease-associated PCNA variants.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Frederick Amankwaah, John Nii Addotey, Emmanuel Orman, Reimmel Adosraku, Isaac Kingley Amponsah
Summary: This study compares the biological and physicochemical properties of propolis extracts from different sources in Ghana. The ethanol-water extract shows the strongest antihyperglycemic activity and contains constituents with strong antioxidant and antidiabetic properties, such as caffeic acid and flavonoid derivatives.
SCIENTIFIC AFRICAN
(2023)
Article
Biochemistry & Molecular Biology
Anna Nickelsen, Claudia Goetz, Florian Lenz, Karsten Niefind, Simone Koenig, Joachim Jose
Summary: This study identified 38 new interaction partners of human CK2β, including HSP70-1, using photo-crosslinking and mass spectrometry. The interaction between HSP70-1 and CK2β was determined to have a K-D value of 0.57 μM. Phosphorylation studies suggested that HSP70-1 does not function as a substrate or activity modulator of CK2, indicating an independent interaction of HSP70-1 with CK2β.
Article
Chemistry, Multidisciplinary
Priscila Lopez-Rojas, Samer Haidar, Franziska M. Juergens, Dagmar Aichele, Angel Amesty, Ana Estevez-Braun, Joachim Jose
Summary: In this study, the inhibitory activity of endocrocin and its derivatives on human protein kinase CK2 was evaluated. Endocrocin was found to be a moderate inhibitor, while only one derivative showed strong inhibitory activity. Furthermore, the effects of emodin, endocrocin, and compound 4i on breast cancer cells (MCF7) were investigated.
RESULTS IN CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Katharina Possart, Fabian C. Herrmann, Joachim Jose, Thomas J. Schmidt
Summary: This article investigates the drug treatment of tropical diseases caused by parasites. Through enzyme analysis and computational screening, several compounds with dual inhibitory effects were identified, which could potentially be used for the development of new drugs.
Article
Chemistry, Multidisciplinary
Tristan Wegner, Alexander Dombovski, Katrin Gesing, Alexander Koehrer, Matthias Elinkmann, Uwe Karst, Frank Glorius, Joachim Jose
Summary: The cell membrane is an important platform for modifying and manipulating cellular behavior. This study presents a method for functionalizing the bacterial membrane using lipid mimetics, allowing for a new type of cascade reaction.
Article
Genetics & Heredity
Maria Asif, Emrah Kaygusuz, Marwan Shinawi, Anna Nickelsen, Tzung-Chien Hsieh, Prerana Wagle, Birgit S. Budde, Jennifer Hochscherf, Uzma Abdullah, Stefan Honing, Christian Nienberg, Dirk Lindenblatt, Angelika A. Noegel, Janine Altmuller, Holger Thiele, Susanne Motameny, Nicole Fleischer, Idan Segal, Lynn Pais, Sigrid Tinschert, Nadra Nasser Samra, Juliann M. Savatt, Natasha L. Rudy, Chiara De Luca, Paola Fortugno, Susan M. White, Peter Krawitz, Anna C. E. Hurst, Karsten Niefind, Joachim Jose, Francesco Brancati, Peter Nurnberg, Muhammad Sajid Hussain
Summary: Mutations in the CSNK2B gene cause a new craniodigital syndrome distinguished from POBINDS by disrupting the canonical Wnt signaling pathway in a dominant-negative manner.
HUMAN GENETICS AND GENOMICS ADVANCES
(2022)
Article
Biochemistry & Molecular Biology
Katharina Possart, Fabian C. Herrmann, Joachim Jose, Maria P. Costi, Thomas J. Schmidt
Summary: This paragraph introduces a new method for treating insomnia, which improves sleep quality by adjusting the alpha wave frequency of the brain.
Article
Chemistry, Medicinal
Saeed Reza Simaei, Vahid Reza Askari, Mahboobeh Rostami, Mohammad Kamalinejad, Mohammad Hosein Farzaei, Mohammadreza Morovati, Fatemeh Heydarpour, Zahra Jafari, Vafa Baradaran Rahimi
Summary: This study demonstrates that lavender and metformin have similar effects in treating PCOS patients, as both significantly increase progesterone levels and regulate menstrual cycles.
Article
Chemistry, Medicinal
Peng-Ran Cao, Xiao Ren, Jie Lin, Yun-Long Mu, Yue-Qi Shan, Jing-Tong Zhu, Rong-Yi Xu, Xue-Xia Zhang, Wei-Guo Hu, Xin-Hua Lu
Summary: Four previously unknown angucyclinones umezawaones A-D (1-4) were isolated from the liquid cultures of Umezawaea beigongshangensis. Their structures were determined using spectroscopic analysis, single crystal X-ray diffraction, quantum chemical 13C NMR, and electronic circular dichroism calculations. All compounds exhibited potent inhibitory activities against indoleamine 2,3-dioxygenase and tryptophan-2,3-dioxygenase in enzymatic assays, with compound 2 showing the highest activity.
Article
Chemistry, Medicinal
Marwa Mersni, Bingqing Zhou, Guillaume Reversat, Mohamed Larbi Khouja, Alexandre Guy, Camille Oger, Jean-Marie Galano, Thierry Durand, Chokri Messaoud, Claire Vigor
Summary: The relevance of oxylipins as biomarkers of oxidative stress has been established in recent years. This study evaluated the lipidomic profiling of different aerial parts of Acacia cyanophylla Lindl. using LC-MS/MS technology for the first time. The analysis revealed the existence of six PhytoPs and three PhytoFs, with stems having the highest amount of these metabolites. This study provides a foundation for exploring Acacia cyanophylla Lindl. as a potential source of plant oxylipins for therapeutic or pharmaceutical uses.
Review
Chemistry, Medicinal
Lorenzo Buzzelli, Andrea Segreti, Daniela Di Gioia, Erika Lemme, Maria Rosaria Squeo, Antonio Nenna, Giuseppe Di Gioia
Summary: This review provides a comprehensive assessment of red yeast rice, including its biochemical composition, historical studies, cardiovascular disease prevention, and other clinical effects. Red yeast rice preparations have been proven safe and effective in improving lipid profiles, with a potential role in reducing cardiovascular risk.
Article
Chemistry, Medicinal
Xiuwen Zheng, Yuxin Zhang, Yinfeng Tan, Yonghui Li, Qianrong Xue, Hailong Li, Xuguang Zhang, Yipeng Pan, Jian Xu, Junqing Zhang
Summary: Alpinia officinarum Hance (AOH) may protect against diabetic gastroparesis (DGP) through multiple mechanisms, including lowering blood glucose levels, increasing gastrointestinal propulsion rate, improving gastric histological morphology, and regulating gut microbiota.
Article
Chemistry, Medicinal
Na Chen, Rui Xie, Jia-pei Chen, Ying-li Zhong, Xian-wen Zhang, Qing-wen Gui, Chun Guo, Hua Yang
Summary: The study clones the SinSyn gene from Sinomenium acutum and analyzes its catalytic activity, confirming the biosynthetic pathway of sinoacutine and discussing its classification and pharmacological action.
Article
Chemistry, Medicinal
Noura Bentarhlia, Badr Eddine Kartah, Mouhcine Fadil, Said El Harkaoui, Bertrand Matthaeus, Oualid Abboussi, Hanaa Abdelmoumen, Omar Bouhnik, Hanae El Monfalouti
Summary: The lipidic extract of Dittrichia viscosa exhibits significant wound healing activity and antimicrobial effects. It is rich in fatty acids such as linolenic acid, oleic acid, and linoleic acid, as well as compounds like gamma-tocopherols and sterols.
Article
Chemistry, Medicinal
Hawraa Zbeeb, Hala Khalifeh, Giulio Lupidi, Francesca Baldini, Lama Zeaiter, Mohamad Khalil, Annalisa Salis, Gianluca Damonte, Laura Vergani
Summary: This study assessed the biological properties of S. spinosum fruits and found that the ethanolic extract exhibited significant anti-steatotic and antioxidant activities.