Article
Biochemistry & Molecular Biology
Romeo Arago Dougue Kentsop, Valeria Iobbi, Giuliana Donadio, Barbara Ruffoni, Nunziatina De Tommasi, Angela Bisio
Summary: Salvia corrugata hairy roots were obtained through transformation, serving as a potential source of abietane and abeo-abietane compounds with antibacterial, antitumor, and cytotoxic activities. The study demonstrated the presence of horminone, ferruginol, 7-O-acetylhorminone, and 7-O-methylhorminone in untransformed roots, while agastol and ferruginol were isolated and quantified from the hairy roots, indicating their potential as bioactive compound sources.
Article
Plant Sciences
Arpine Ayvazyan, Lenard Deutsch, Christian Zidorn, Brigitte Kircher, Serhat S. Cicek
Summary: This study compared the tanshinone profiles of Salvia miltiorrhiza roots and Salvia glutinosa roots and found that S. miltiorrhiza had higher concentrations of most tanshinones. However, S. glutinosa contained danshenol A, dehydroabietic acid, and (+)-pisiferic acid, which showed strong antiproliferative and cytotoxic effects. These findings highlight the potential value of investigating closely related species for the discovery of new biologically active lead compounds.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Jiao-Jiao Song, Xin Fang, Chen-Yi Li, Yan Jiang, Jian-Xu Li, Sheng Wu, Juan Guo, Yan Liu, Hang Fan, Yan-Bo Huang, Yu-Kun Wei, Yu Kong, Qing Zhao, Jing-Jing Xu, Yong-Hong Hu, Xiao-Ya Chen, Lei Yang
Summary: A 2-oxoglutarate-dependent dioxygenase (2-ODD) gene responsible for tanshinone IIA biosynthesis in Salvia miltiorrhiza was identified in this study. The gene, known as Sm2-ODD14, acts as a dehydrogenase and catalyzes the aromatization of the furan ring. The study also showed that Sm2-ODD14 controls the metabolite flux from dihydrofuran- to furan-tanshinones, which impacts the medicinal properties of S. miltiorrhiza.
Article
Plant Sciences
Yuekai Su, Caicai Lin, Jin Zhang, Bei Hu, Jie Wang, Jingyu Li, Shiqi Wang, Ruihao Liu, Xia Li, Zhenqiao Song, Jianhua Wang
Summary: A novel one-step hairy root regeneration method was developed for Salvia miltiorrhiza, resulting in high tanshinone content in regenerated plants. This method provides a foundation for breeding S. miltiorrhiza and sustainable development of medicinal plant resources, serving as a useful reference for the application of other species.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Multidisciplinary Sciences
Bo-Cheng Yang, Meng-Shiou Lee, Ming-Kuem Lin, Wen-Te Chang
Summary: Recent studies have found strong connections between epigenetic modulation and secondary metabolites in plants. In this study, the effects of the DNA methylation inhibitor 5-azacytidine (5-Az) on secondary metabolite production, gene expression, and DNA methylation levels were investigated in the hairy roots of the medicinal plant Salvia miltiorrhiza. Results showed that the content of tanshinones, a type of secondary metabolite, increased by 1.5-5 times in the hairy roots treated with 5-Az. The methylation pattern in the promoter region of the gene encoding copalyl diphosphate synthase (CPS) was altered during 5-Az treatment, and several transcription factor binding sites were identified. This study provides evidence for a possible mechanism of DNA methylation in regulating tanshinone biosynthesis in the hairy roots of S. miltiorrhiza.
SCIENTIFIC REPORTS
(2022)
Article
Plant Sciences
Abdukriem Kadir, Guijuan Zheng, Xiaofeng Zheng, Pengfei Jin, Maitinuer Maiwulanjiang, Biao Gao, Haji Akber Aisa, Guangmin Yao
Summary: Twenty-four diterpenoids were isolated from the roots of Salvia deserta, with 10 new compounds named salviadesertins A-J. The structures were determined using spectroscopic data interpretation, quantum-chemical calculations, and X-ray crystallography analysis. Cytotoxicity evaluation revealed 6-hydroxysalvinolone (Compound 14) showed potential against multiple cancer cell lines.
JOURNAL OF NATURAL PRODUCTS
(2021)
Article
Biochemistry & Molecular Biology
Zahra Alizadeh, Giuliana Donadio, Mahdi Moridi Farimani, Valentina Parisi, Samad Nejad Ebrahimi, Nunziatina De Tommasi
Summary: Two seco-norabietane diterpenes, abrotafuran and abrotacid, were isolated from Salvia abrotanoides roots. Abrotafuran exhibited antiproliferative activity on HeLa and Jurkat cell lines.
Article
Plant Sciences
Huaiyu Deng, Qi Li, Ruizhi Cao, Yafei Ren, Guanfeng Wang, Hongbo Guo, Shuhai Bu, Jingying Liu, Pengda Ma
Summary: Soil salinity has a significant impact on the growth and development of Salvia miltiorrhiza, as well as seed germination. The study reveals that the transcription factor MYC2 plays a crucial role in plant stress resistance. Overexpression of SmMYC2 enhances salt tolerance in transgenic plants by regulating the expression of antioxidant enzymes, proline, and flavonoid synthesis genes.
JOURNAL OF PLANT PHYSIOLOGY
(2023)
Article
Plant Sciences
Meiling Ding, Bin Zhang, Shuo Zhang, RongRong Hao, Yu Xia, Pengda Ma, Juane Dong
Summary: Phenolic acids are the main bioactive compounds in Salvia miltiorrhiza, and their production can be increased by salicylic acid (SA) treatment. In this study, a negative regulator of SA-induced phenolic acid biosynthesis, SmNPR4, was identified in S. miltiorrhiza hairy roots. This study also discovered a transcription factor, SmTGA5, that interacts with SmNPR4 and activates the expression of the phenolic acid biosynthesis gene SmTAT1. The results showed that SA can alleviate the inhibitory effect of SmNPR4 on SmTGA5. These findings enhance our understanding of the molecular mechanism behind salicylic acid regulation of phenolic acid biosynthesis in S. miltiorrhiza.
HORTICULTURE RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Nan -Fang Zhong, Hui -Hui Huang, Jiang -Chun Wei, Yong-Cheng Yang, Xiao-Xu Gao, Xiu-Yan Wei, An-Hua Wang, Jing-Ming Jia
Summary: Nine previously undescribed diterpenoids and seven known diterpenoids were isolated from the roots of wild Euphorbia fischeriana. The structures were analyzed and determined, and their configuration was confirmed. These compounds were tested for their antitumor effects, and some of them showed inhibitory effects on tumor cells.
Article
Chemistry, Physical
Somayeh Zare, Gholamreza Hatam, Omidreza Firuzi, Azam Bagheri, Jima N. Chandran, Bernd Schneider, Christian Paetz, Somayeh Pirhadi, Amir Reza Jassbi
Summary: Four abietane diterpenoids were isolated from the root extract of Salvia hydrangea, with compound 4 showing considerable antileishmanial activity. The root extract also exhibited significant cytotoxicity against two cancer cell lines. A pharmacophore modeling study revealed essential features for effective inhibition of antileishmanial activity.
JOURNAL OF MOLECULAR STRUCTURE
(2021)
Article
Biochemistry & Molecular Biology
Elham Sarhadi, Samad Nejad Ebrahimi, Abbas Hadjiakhoondi, Mahdi Abbas-Mohammadi, Azadeh Manayi, Valentina Parisi, Emanuela Pessolano, Antonello Petrella, Nunziatina De Tommasi
Summary: The phytochemical profiling of the root extract of Salvia leriifolia, an endemic plant of Iran, was investigated. Sixteen abietane diterpenes were isolated, including three original compounds. The structural elucidation was performed using 1D and 2D NMR and HRMS, and the absolute configuration of previously unreported compounds was determined using circular dichroism (ECD). The isolated compounds were evaluated for their cytotoxicity against AGS, MIA PaCa-2, HeLa, and MCF-7 cell lines using the MTT assay. One of the known diterpenes, pisiferal, exhibited high cytotoxicity against all tested cell lines at concentrations ranging from 9.3 +/- 0.6 to 14.38 +/- 1.4 mu M.
Article
Plant Sciences
Qi Li, Xin Fang, Ying Zhao, Ruizhi Cao, Juane Dong, Pengda Ma
Summary: This study found that SmMYB36 promotes the accumulation of tanshinones and suppresses the synthesis of phenolic acids through the regulation of key enzyme genes and other pathways. It was also discovered that SmERF6 has a promoting effect on tanshinone synthesis, while SmERF115 has an inhibitory effect on phenolic acid synthesis. This study contributes to revealing the potential role of transcription factors in the regulation of tanshinone and phenolic acid biosynthesis.
HORTICULTURE RESEARCH
(2023)
Review
Chemistry, Multidisciplinary
Mariaevelina Alfieri, Alfredo Ambrosone, Mariacarmela Vaccaro, Nunziatina De Tommasi, Antonietta Leone
Summary: This review summarizes the efforts and results of optimizing the synthesis of abietane diterpenoids in Salvia sclarea hairy roots. Various approaches such as elicitation and gene expression modification were used to increase the content of these compounds in the roots.
APPLIED SCIENCES-BASEL
(2022)
Article
Plant Sciences
Wenjuan Xu, Xiaoyan Jin, Miao Yang, Song Xue, Linglong Luo, Xupeng Cao, Caijuan Zhang, Sanyang Qiao, Chi Zhang, Junling Li, Jiahui Wu, Liqiao Lv, Fangyuan Zhao, Nan Wang, Shuting Tan, A. Ga Lyu-Bu, Chunguo Wang, Xueyong Wang
Summary: The study revealed that the endophytic fungus Mucor fragilis functions as an effective elicitor for improving the accumulation of primary and secondary metabolites in Salvia miltiorrhiza hairy roots by regulating gene expression. This finding provides guidance for quality control of S. miltiorrhiza and highlights the potential application of Mucor fragilis as an effective endophytic fungal elicitor.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Plant Sciences
Izabela Weremczuk-Jezyna, Ewa Kochan, Piotr Szymczyk, Pawel Lisiecki, Lukasz Kuzma, Izabela Grzegorczyk-Karolak
PHYTOCHEMISTRY LETTERS
(2019)
Article
Plant Sciences
Izabela Grzegorczyk-Karolak, Lukasz Kuzma, Pawel Lisiecki, Anna Kiss
PHYTOCHEMISTRY LETTERS
(2019)
Article
Agricultural Engineering
Ewelina Piatczak, Agnieszka Jelen, Joanna Makowczynska, Sylwia Zielinska, Lukasz Kuzma, Ewa Balcerczak
INDUSTRIAL CROPS AND PRODUCTS
(2019)
Article
Biotechnology & Applied Microbiology
Izabela Weremczuk-Jezyna, Ewa Skala, Lukasz Kuzma, Anna K. Kiss, Izabela Grzegorczyk-Karolak
JOURNAL OF BIOTECHNOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Izabela Grzegorczyk-Karolak, Katarzyna Hnatuszko-Konka, Mariola Zarzycka, Lukasz Kuzma
Article
Biochemistry & Molecular Biology
Izabela Weremczuk-Jezyna, Pawel Lisiecki, Weronika Gonciarz, Lukasz Kuzma, Magdalena Szemraj, Magdalena Chmiela, Izabela Grzegorczyk-Karolak
Article
Agricultural Engineering
Ewelina Piatczak, Lukasz Kuzma, Weronika Kozlowska, Pawel Lisiecki, Magdalena Szemraj, Bartosz J. Plachno, Weronika Gonciarz, Magdalena Chmiela, Adam Matkowski, Sylwia Zielinska
INDUSTRIAL CROPS AND PRODUCTS
(2020)
Article
Biochemistry & Molecular Biology
Izabela Weremczuk-Jezyna, Lukasz Kuzma, Izabela Grzegorczyk-Karolak
Summary: The study found that blue and red/blue light had the strongest impact on morphogenesis and shoot propagation, resulting in more than five new shoots per explant. Blue light cultures had the highest fresh and dry weights, polyphenol levels, and antioxidant potential compared to other light conditions. LED culture proved to be beneficial for enhancing the medicinal value of transformed D. forrestii shoot culture.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Jadwiga Nowak, Anna K. Kiss, Charles Wambebe, Esther Katuura, Lukasz Kuzma
Summary: This study employed chromatographic and spectroscopic techniques to identify and quantify polyphenolic compounds in Vernonia amygdalina leaf extract. The results revealed the presence of seven polyphenols, with 3,5-O-dicaffeoylquinic acid being the most abundant. These polyphenolic compounds may have therapeutic potential in terms of antibacterial, cytotoxic, anticancer, and antioxidant effects.
APPLIED SCIENCES-BASEL
(2022)
Article
Biochemistry & Molecular Biology
Piotr Szymczyk, Grazyna Szymanska, Lukasz Kuzma, Agnieszka Jelen, Ewa Balcerczak
Summary: This study characterized the 5' regulatory region of the SmMEC gene and identified MeJa-responsive cis-active motifs. Co-expression studies and RT-PCR data confirmed the significant role of MeJa in SmMEC gene regulation. Furthermore, the experiment with S. miltiorrhiza solid callus cultures showed biphasic tanshinone accumulation after MeJa treatment.
Article
Biochemistry & Molecular Biology
Malgorzata Majewska, Piotr Szymczyk, Jan Gomulski, Agnieszka Jelen, Renata Grabkowska, Ewa Balcerczak, Lukasz Kuzma
Summary: This study reveals the expression profile of the S. miltiorrhiza HMGR4 gene in different organs and its sensitivity to gibberellic acid, indole-3-acetic acid, and salicylic acid. The overexpression of HMGR4 significantly increases the accumulation of tanshinones, and hormones, plant organs, and culture environment play important roles in tanshinone biosynthesis.
Article
Plant Sciences
Malgorzata Majewska, Lukasz Kuzma, Piotr Szymczyk
Summary: Salvia miltiorrhiza synthesises tanshinones with multidirectional therapeutic effects. In this study, a new sequence of the HMGR4 gene in S. miltiorrhiza was isolated and characterised, providing valuable information for understanding the transcription regulation of this gene and further exploring the medicinal value of S. miltiorrhiza.
Review
Biochemistry & Molecular Biology
Lukasz Kuzma, Jan Gomulski
Summary: Pharmacognosy is a field of study that focuses on the diterpenoids, and Clerodendrum plants are an important source of these compounds. The diterpenoids isolated from Clerodendrum demonstrate a range of biological activities, including cytotoxic, antibacterial, and anti-inflammatory effects. Therefore, the study of diterpenoids from Clerodendrum plants is important for drug discovery and development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Katarzyna Syklowska-Baranek, Malgorzata Gawel, Lukasz Kuzma, Beata Wilenska, Mateusz Kawka, Malgorzata Jeziorek, Konstantia Graikou, Ioanna Chinou, Ewa Szyszko, Piotr Stepien, Patryk Zakrzewski, Agnieszka Pietrosiuk
Summary: The in vitro cultures of Rindera graeca were developed to provide a sustainable source of phenolic acids. Both shoot and root cultures were established and yielded high levels of rosmarinic acid and lithospermic acid B. The highest antioxidant activity was observed in roots cultivated in DCR medium, while the highest reducing power was observed in shoots cultured on an SH medium with 0.5 mg/L 6-benzylaminopurine. Genetic analysis showed significant variation among the investigated shoots and roots, indicating the ability of cultivated tissues to produce phenolic compounds.
Review
Plant Sciences
Agnieszka Pietrosiuk, Anna Budzianowska, Jaromir Budzianowski, Halina Ekiert, Malgorzata Jeziorek, Anna Kawiak, Malgorzata Kikowska, Miroslawa Krauze-Baranowska, Aleksandra Krolicka, Lukasz Kuzma, Maria Luczkiewicz, Janusz Malarz, Adam Matkowski, Anna Stojakowska, Katarzyna Syklowska-Baranek, Agnieszka Szopa, Wojciech Szypula, Barbara Thiem, Sylwia Zielinska
Summary: Plant cell and organ cultures are potential sources of valuable secondary metabolites that can be used in various industries. Poland has shown interest in applying plant biotechnology for pharmaceutical purposes, utilizing in vitro cultures to produce known and novel compounds.
ACTA SOCIETATIS BOTANICORUM POLONIAE
(2022)
Article
Chemistry, Medicinal
Saeed Reza Simaei, Vahid Reza Askari, Mahboobeh Rostami, Mohammad Kamalinejad, Mohammad Hosein Farzaei, Mohammadreza Morovati, Fatemeh Heydarpour, Zahra Jafari, Vafa Baradaran Rahimi
Summary: This study demonstrates that lavender and metformin have similar effects in treating PCOS patients, as both significantly increase progesterone levels and regulate menstrual cycles.
Article
Chemistry, Medicinal
Peng-Ran Cao, Xiao Ren, Jie Lin, Yun-Long Mu, Yue-Qi Shan, Jing-Tong Zhu, Rong-Yi Xu, Xue-Xia Zhang, Wei-Guo Hu, Xin-Hua Lu
Summary: Four previously unknown angucyclinones umezawaones A-D (1-4) were isolated from the liquid cultures of Umezawaea beigongshangensis. Their structures were determined using spectroscopic analysis, single crystal X-ray diffraction, quantum chemical 13C NMR, and electronic circular dichroism calculations. All compounds exhibited potent inhibitory activities against indoleamine 2,3-dioxygenase and tryptophan-2,3-dioxygenase in enzymatic assays, with compound 2 showing the highest activity.
Article
Chemistry, Medicinal
Marwa Mersni, Bingqing Zhou, Guillaume Reversat, Mohamed Larbi Khouja, Alexandre Guy, Camille Oger, Jean-Marie Galano, Thierry Durand, Chokri Messaoud, Claire Vigor
Summary: The relevance of oxylipins as biomarkers of oxidative stress has been established in recent years. This study evaluated the lipidomic profiling of different aerial parts of Acacia cyanophylla Lindl. using LC-MS/MS technology for the first time. The analysis revealed the existence of six PhytoPs and three PhytoFs, with stems having the highest amount of these metabolites. This study provides a foundation for exploring Acacia cyanophylla Lindl. as a potential source of plant oxylipins for therapeutic or pharmaceutical uses.
Review
Chemistry, Medicinal
Lorenzo Buzzelli, Andrea Segreti, Daniela Di Gioia, Erika Lemme, Maria Rosaria Squeo, Antonio Nenna, Giuseppe Di Gioia
Summary: This review provides a comprehensive assessment of red yeast rice, including its biochemical composition, historical studies, cardiovascular disease prevention, and other clinical effects. Red yeast rice preparations have been proven safe and effective in improving lipid profiles, with a potential role in reducing cardiovascular risk.
Article
Chemistry, Medicinal
Xiuwen Zheng, Yuxin Zhang, Yinfeng Tan, Yonghui Li, Qianrong Xue, Hailong Li, Xuguang Zhang, Yipeng Pan, Jian Xu, Junqing Zhang
Summary: Alpinia officinarum Hance (AOH) may protect against diabetic gastroparesis (DGP) through multiple mechanisms, including lowering blood glucose levels, increasing gastrointestinal propulsion rate, improving gastric histological morphology, and regulating gut microbiota.
Article
Chemistry, Medicinal
Na Chen, Rui Xie, Jia-pei Chen, Ying-li Zhong, Xian-wen Zhang, Qing-wen Gui, Chun Guo, Hua Yang
Summary: The study clones the SinSyn gene from Sinomenium acutum and analyzes its catalytic activity, confirming the biosynthetic pathway of sinoacutine and discussing its classification and pharmacological action.
Article
Chemistry, Medicinal
Noura Bentarhlia, Badr Eddine Kartah, Mouhcine Fadil, Said El Harkaoui, Bertrand Matthaeus, Oualid Abboussi, Hanaa Abdelmoumen, Omar Bouhnik, Hanae El Monfalouti
Summary: The lipidic extract of Dittrichia viscosa exhibits significant wound healing activity and antimicrobial effects. It is rich in fatty acids such as linolenic acid, oleic acid, and linoleic acid, as well as compounds like gamma-tocopherols and sterols.
Article
Chemistry, Medicinal
Hawraa Zbeeb, Hala Khalifeh, Giulio Lupidi, Francesca Baldini, Lama Zeaiter, Mohamad Khalil, Annalisa Salis, Gianluca Damonte, Laura Vergani
Summary: This study assessed the biological properties of S. spinosum fruits and found that the ethanolic extract exhibited significant anti-steatotic and antioxidant activities.
