Article
Reproductive Biology
Vahid Najafzadeh, Jan Bojsen-Moller Secher, Maria Pihl, Anna AErenlund, Natasha Jorgensen, Karoline Kjaersgaard Jensen, Maibritt Traeholt Jensen, Merle Friederike Fenner, Lotte Strobech, Poul Hyttel
Summary: The study revealed that vitrification resulted in a higher cryo-survival rate compared to slow freezing in biopsied bovine IVP blastocysts. Both biopsy-vitrification and biopsy-slow-freeze showed similar results in terms of ICM, TE, and total blastocyst cell numbers, as well as cleaved caspase-3 and TUNEL-positive cell rates. Biopsy and cryopreservation alone had no effect on cell numbers, while slow freezing resulted in increased cleaved caspase-3 positive cell rates. Double traumatization with a combination of biopsy and cryopreservation increased cleaved caspase-3 and TUNEL-positive cells rates.
Article
Obstetrics & Gynecology
Molly M. Quinn, Philip Marsh, Salustiano Ribeiro, Rhodel K. Simbulan, Cristina Hickman, Jorgen Berntsen, Mitchell P. Rosen
Summary: Embryo quality, morphokinetic parameters, and aneuploidy rates were found to be similar in sibling embryos cultured in distinct media systems from the time of gamete isolation.
HUMAN REPRODUCTION
(2022)
Article
Endocrinology & Metabolism
Florence Belva, Fiskani Kondowe, Anick De Vos, Kathelijn Keymolen, Andrea Buysse, Frederik Hes, Veerle Berckmoes, Pieter Verdyck, Willem Verpoest, Martine De Rycke
Summary: This study found that embryo biopsy (EBD3 or EBD5) followed by fresh or frozen-thawed embryo transfer did not have a negative impact on anthropometry and health outcomes up to 2 years of age.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2023)
Article
Multidisciplinary Sciences
Xue Wang, Yaling Xiao, Yuanzheng Zhou, Hanbi Wang
Summary: The purpose of this study was to investigate whether the development speed of sibling embryos is positively correlated with the live birth rate following fresh embryo transfer. The study examined 1262 cycles of women who underwent day 3 (D3) cleavage embryo transfer. The results showed that the live birth rate was significantly lower in patients with blastocysts that formed on D6 compared to the other two groups.
SCIENTIFIC REPORTS
(2023)
Article
Obstetrics & Gynecology
Tetsuya Miki, Kenji Ezoe, Shizu Kouraba, Kazuki Ohata, Keiichi Kato
Summary: The time interval from trophectoderm biopsy to vitrification significantly affects the adhesion and outgrowth ability of blastocysts. Vitrification should be performed immediately after biopsy to enhance the outgrowth competence of blastocysts.
REPRODUCTIVE MEDICINE AND BIOLOGY
(2022)
Article
Genetics & Heredity
Andrea Abdala, Ibrahim Elkhatib, Asina Bayram, Ana Arnanz, Ahmed El-Damen, Laura Melado, Barbara Lawrenz, Carol Coughlan, Nicolas Garrido, Human M. Fatemi, Neelke De Munck
Summary: Under controlled in vitro conditions, euploidy rates and embryo development are comparable when embryos are cultured in CCM or SCM.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2021)
Article
Reproductive Biology
Nuria Gonzalez-Rodriguez, Iris Martinez-Rodero, Jakob Scherzer, Simone Jung, Myriam Reichenbach, Yury Zablotski, Christiane Otzdorff, Holm Zerbe, Teresa Mogas
Summary: In the cattle industry, using genotyping and cryopreservation technologies combined with in vivo or in vitro embryo production allows the selection and preservation of embryos with desirable traits. This study assessed the efficiency of a vitrification method for in-straw warming of biopsied bovine embryos. Results showed that both vitrification methods produced similar outcomes for intact and biopsied embryos, and vitrification did not affect the viability of biopsied embryos to develop into pregnancies.
Article
Biochemistry & Molecular Biology
Katherine L. Palmerola, Selma Amrane, Alejandro De los Angeles, Shuangyi Xu, Ning Wang, Joao de Pinho, Michael Zuccaro, Angelo Taglialatela, Dashiell J. Massey, Jenna Turocy, Alex Robles, Anisa Subbiah, Bob Prosser, Rogerio Lobo, Alberto Ciccia, Amnon Koren, Timour Baslan, Dieter Egli
Summary: This study reveals that DNA replication stress in human preimplantation embryos predisposes gene-poor regions to fragility, resulting in the formation of aneuploidies.
Article
Biology
Martin Stimpfel, Nina Jancar, Helena Ban-Frangez, Eda Vrtacnik-Bokal
Summary: The latest reports suggest that it may be better to transfer embryos to the uterus on day five instead of day three of preimplantation development, but this may only be applicable when patients are under 36 years old and there are only one-two embryos obtained in the cycle. A retrospective study was conducted to address this issue, analyzing data from IVF/ICSI cycles where one-two embryos were obtained and comparing the outcomes between day three and day five embryo transfer. The study revealed that the birth rate per embryo transfer was significantly higher in the day five group, particularly in patients under 36 years old.
Article
Medicine, Research & Experimental
Yidong Chen, Yuan Gao, Jialin Jia, Liang Chang, Ping Liu, Jie Qiao, Fuchou Tang, Lu Wen, Jin Huang
Summary: The discovery of embryonic cell-free DNA in spent embryo culture media has provided hope for noninvasive preimplantation genetic testing. However, the cellular origins of this DNA are not well understood, and methods for detecting maternal DNA contamination are limited. This study used whole-genome DNA methylation sequencing to identify the sources of cell-free DNA in spent embryo culture media, providing insights into noninvasive preimplantation genetic testing.
JOURNAL OF CLINICAL INVESTIGATION
(2021)
Article
Obstetrics & Gynecology
Fei Li, He Cai, Li Tian, Haiyan Bai, Juanzi Shi
Summary: Late follicular phase progesterone elevation (LFPE) during ovarian stimulation is associated with reduced live birth rates (LBRs) after cleavage-stage embryo transfer. However, due to better synchronization with a stimulated endometrium, prior studies shown that LFPE had no effect on transferring embryos at blastocyst stage. The study aim to exam whether the developmental stage of embryos and serum progesterone levels on the day of human chorionic gonadotropin (hCG) administration jointly affect the odds of live birth in fresh fresh IVF/intracytoplasmic sperm injection (ICSI) cycles.
BMC PREGNANCY AND CHILDBIRTH
(2023)
Article
Genetics & Heredity
Roni Rahav-Koren, Shmuel Inbar, Netanella Miller, Amir Wiser, Yael Yagur, Chen Berkowitz, Sivan Farladansky-Gershnabel, Adrian Shulman, Arie Berkowitz
Summary: The study suggests that culturing post-thaw cleavage embryos for 2 days and transferring them as blastocysts may increase the chances of ongoing pregnancy and delivery compared to transferring thawed blastocysts directly.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2021)
Article
Obstetrics & Gynecology
Katerina Chatzimeletiou, Antonia Sioga, Nikos Petrogiannis, Yannis Panagiotidis, Marialena Prapa, Antonios Patrikiou, Basil C. Tarlatzis, Grigoris Grimbizis
Summary: The study found that vitrified embryos had higher cell damage compared to fresh embryos, regardless of whether they were vitrified in open or closed systems. However, both Day 5 and Day 3 biopsied embryos showed no adverse effects on viability and ultrastructure following vitrification in open and closed systems.
REPRODUCTIVE BIOMEDICINE ONLINE
(2021)
Review
Genetics & Heredity
Antonio Capalbo, Maurizio Poli, Chaim Jalas, Eric J. Forman, Nathan R. Treff
Summary: The use of aneuploidy testing in IVF cycles is increasing, with almost half of cycles in the US undergoing PGT-A. Evidence shows that embryos diagnosed with uniform whole-chromosome aneuploidy rarely result in live births, while embryos with low-level mosaicism for whole chromosomes have reproductive capabilities similar to euploid embryos. Differentiating between uniform and mosaic aneuploidy is crucial in clinical and research settings for proper management of aneuploid embryos.
AMERICAN JOURNAL OF HUMAN GENETICS
(2022)
Article
Endocrinology & Metabolism
Ying Zhao, Dong'e Liu, Nenghui Liu, Yumei Li, Zhongyuan Yao, Fen Tian, Aizhuang Xu, Yanping Li
Summary: This study investigates the endometrial characteristics most likely to induce ectopic pregnancy in frozen-thawed embryo transfers. The risk of ectopic pregnancy significantly increases when the endometrial thickness is less than 8 mm. Thin endometrial thickness may be related to abnormal endometrial peristaltic waves or abnormal endometrial receptivity. Adequate attention should be paid to patients with thin endometrial thickness to prevent ectopic pregnancy or achieve early diagnosis.
FRONTIERS IN ENDOCRINOLOGY
(2022)
