Article
Multidisciplinary Sciences
Krishnendu Khan, Briana Long, Valentin Gogonea, Gauravi M. Deshpande, Kommireddy Vasu, Paul L. Fox
Summary: Aminoacyl-tRNA synthetases (aaRSs) are essential enzymes in the translation process that ligate amino acids to their corresponding transfer RNAs (tRNAs). In mammalian cells, these enzymes, along with AIMPs proteins, form a large multi-tRNA synthetase complex (MSC), whose assembly mechanism and function are still unclear. This study reveals the importance of cotranslational interactions, particularly involving AIMPs proteins, in the assembly process of the MSC. Interestingly, these cotranslational interactions sometimes involve more than two proteins, suggesting a diverse pathway for the ordered assembly of small subcomplexes into larger complexes.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Jose R. Jaramillo Ponce, Anne Theobald-Dietrich, Philippe Benas, Caroline Paulus, Claude Sauter, Magali Frugier
Summary: tRip is a specific tRNA import protein for Plasmodium. It can associate with three aminoacyl-tRNA synthetases (aaRS) and form multi-aaRS complexes (MSC) in the parasite. The crystal structure of the N-terminal GST-like domain of one of the aaRS was solved, allowing further investigation of the solution architecture of the complexes. The biological impact of these structural arrangements is discussed.
Article
Oncology
Krishnendu Khan, Valentin Gogonea, Paul L. Fox
Summary: Aminoacyl-tRNA synthetases (AARS) are important enzymes in mammalian cells that play a key role in protein translation. Recent studies have found that they exist in the cytoplasmic multi-tRNA synthetase complex (MSC) and have non-canonical functions in addition to their role in protein translation. These findings have the potential to be new therapeutic targets for cancer and other diseases.
TRANSLATIONAL ONCOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jessica M. Warren, Amanda K. Broz, Ana Martinez-Hottovy, Christian Elowsky, Alan C. Christensen, Daniel B. Sloan
Summary: The number of tRNAs encoded in plant mitochondrial genomes varies considerably. The loss of bacterial-like mitochondrial tRNA genes necessitates the import of nuclear-encoded counterparts with little sequence similarity. The evolution of aaRS subcellular localization in Sileneae reveals differing constraints on tRNA/aaRS interactions and alternative coevolutionary paths for maintaining organellar translation in plant cells.
MOLECULAR BIOLOGY AND EVOLUTION
(2023)
Letter
Immunology
Yoshinao Muro, Yuta Yamashita, Haruka Koizumi, Mariko Ogawa-Momohara, Takuya Takeichi, Teruyuki Mitsuma, Masashi Akiyama
Summary: Anti-aminoacyl-tRNA synthetase (anti-ARS) antibodies are helpful in identifying inflammatory myopathy patients. In a study with Japanese patients, autoantibodies against CysARS and ValARS were found in the serum of two dermatomyositis patients. One patient showed features of anti-synthetase syndrome, while the other did not. Further research is needed to explore the clinical differences among different anti-ARS antibodies.
AUTOIMMUNITY REVIEWS
(2022)
Article
Biochemistry & Molecular Biology
Siqi Wu, Li Zheng, Zhoufei Hei, Jing-Bo Zhou, Guang Li, Peifeng Li, Jiayuan Wang, Hamid Ali, Xiao-Long Zhou, Jing Wang, Pengfei Fang
Summary: The structures of human Lysyl-tRNA synthetases (LysRSs) are more dynamic than those from single-celled organisms. Without the presence of MSC scaffold proteins, human LysRS can exist independently from the multi-tRNA synthetase complex (MSC). The interaction with the scaffold protein AIMP2 stabilizes the closed conformation of LysRS and protects its essential aminoacylation activity under stressed conditions. Deleting AIMP2 from human cells leads to slow cell growth in nutrient deficient mediums. These results suggest that the evolutionary emergence of the MSC in metazoan might be to protect the aminoacyl-tRNA synthetase components from being modified or recruited for use in other cellular pathways.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Review
Genetics & Heredity
Tamara L. Hendrickson, Whitney N. Wood, Udumbara M. Rathnayake
Summary: The selection of the twenty amino acids in the standard genetic code and the fixation of aminoacyl-tRNA synthetases (aaRSs) before LUCA were influenced by the chemical reactivity of amino acid side chains. Some amino acid properties delayed or prohibited the emergence of corresponding aaRSs, playing critical roles in defining the amino acids in the genetic code.
Review
Immunology
Angeles S. Galindo-Feria, Antonella Notarnicola, Ingrid E. Lundberg, Begum Horuluoglu
Summary: Anti-synthetase syndrome is an autoimmune disease characterized by the presence of autoantibodies targeting aminoacyl t-RNA synthetases along with various clinical features. This review summarizes the functions of aaRSs, their autoantigenic properties, and their association with ASSD.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Swadha Gupta, Jaykumar Jani, Jigneshkumar Vijayasurya, Jigneshkumar Mochi, Saba Tabasum, Akash Sabarwal, Anju Pappachan
Summary: Aminoacyl-tRNA synthetases (AaRSs) play essential roles in translating genetic information and have diverse functions in cellular activities such as transcription, apoptosis, angiogenesis, inflammation, and cancer. Their multifunctionality suggests their potential as therapeutic targets.
Article
Neurosciences
Ling-yue Kong, Yi-ze Wu, Run-qi Cheng, Pei-han Wang, Bi-wen Peng
Summary: Mitochondria play a vital role in cellular energy production and contain mitochondrial DNA (mt DNA) responsible for synthesizing respiratory chain components. Dysfunctions in mt DNA translation have been linked to various syndromes, but their precise mechanisms and implications remain to be determined. Mitochondrial tRNAs (mt tRNAs) encoded by mt DNA are known to contribute to mitochondrial dysfunction and are associated with a wide range of pathologies, including epilepsy. This review focuses on the function of mt tRNA and the role of mitochondrial aminoacyl-tRNA synthetase (mt aaRS) in summarizing common mt aaRS mutant genes causing epilepsy and the specific symptoms associated with these diseases.
MOLECULAR NEUROBIOLOGY
(2023)
Article
Medicine, General & Internal
Kyungjong Lee, Mijung Oh, Kyo-Sun Lee, Yoon Jin Cha, Yoon Soo Chang
Summary: The combination of MARS staining with conventional cytology showed increased diagnostic accuracy for diagnosing lung nodules suspected of lung cancer on chest-computed tomography scans.
Review
Biochemistry & Molecular Biology
Jyoti Chhibber-Goel, Manickam Yogavel, Amit Sharma
Summary: Malaria, caused by parasitic protists, affects over 3 billion people globally with drug resistance posing a major threat to treatment. Targeting aminoacyl-tRNA synthetases (aaRSs) as antimalarial drug targets shows promising potential in killing malaria parasites selectively. This approach highlights the need for new alternatives in malaria treatment to combat drug resistance.
Article
Microbiology
Jingjing Wang, Alexander Berestetskiy, Qiongbo Hu
Summary: Destruxin A (DA) is a mycotoxin produced by the fungus Metarhizium anisopliae with insecticidal activities, showing affinity to the six aminoacyl tRNA synthetases of Bombyx mori. This interaction inhibits protein synthesis and decreases free amino acid content in cells. The study provides insights into the molecular target of DA against target insects.
Article
Biophysics
Xue Sun, Qinggang Li, Yu Wang, Wenjuan Zhou, Yanmei Guo, Jiuzhou Chen, Ping Zheng, Jibin Sun, Yanhe Ma
Summary: In this study, a new strategy for constructing whole-cell biosensors based on aminoacyl-tRNA synthetases (aaRSs) was proposed. By engineering aaRSs, the biosensors showed specificity and potential for developing sensors for various amino acids.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Biochemistry & Molecular Biology
Takumi Yokosawa, Keisuke Wakasugi
Summary: The study shows that Trp-deficient condition is critical for Trp uptake, not only in cells with added TrpRS protein but also in TrpRS-overexpressing cells. Tryptophanyl-AMP production by TrpRS is essential for high-affinity Trp uptake.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemical Research Methods
Marianne Ibrahim, Rabah Gahoual, Ludovic Enkler, Hubert Dominique Becker, Johana Chicher, Philippe Hammann, Yannis-Nicolas Francois, Lauriane Kuhn, Emmanuelle Leize-Wagner
JOURNAL OF CHROMATOGRAPHIC SCIENCE
(2016)
Article
Multidisciplinary Sciences
Ludovic Enkler, Delphine Richer, Anthony L. Marchand, Dominique Ferrandon, Fabrice Jossinet
SCIENTIFIC REPORTS
(2016)
Article
Biochemical Research Methods
Sylvain Debard, Gaetan Bader, Johan-Owen De Craene, Ludovic Enkler, Severine Bar, Daphne Laporte, Philippe Hammann, Evelyne Myslinski, Bruno Senger, Sylvie Friant, Hubert Dominique Becker
Correction
Multidisciplinary Sciences
Anke Harsman, Silke Oeljeklaus, Christoph Wenger, Jonathan L. Huot, Bettina Warscheid, Andre Schneider
NATURE COMMUNICATIONS
(2017)
Article
Biochemistry & Molecular Biology
Nathaniel Yakobov, Sylvain Debard, Frederic Fischer, Bruno Senger, Hubert Dominique Becker
BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS
(2018)
Article
Multidisciplinary Sciences
Jonathan L. Huot, Shikha Shikha, Andre Schneider
SCIENTIFIC REPORTS
(2019)
Article
Genetics & Heredity
Andreea Manole, Stephanie Efthymiou, Emer O'Connor, Marisa Mendes, Matthew Jennings, Reza Maroofian, Indran Davagnanam, Kshitij Mankad, Maria Rodriguez Lopez, Vincenzo Salpietro, Ricardo Harripaul, Lauren Badalato, Jagdeep Walia, Christopher S. Francklyn, Alkyoni Athanasiou-Fragkouli, Roisin Sullivan, Sonal Desai, Kristin Baranano, Faisal Zafar, Nuzhat Rana, Muhammed Ilyas, Alejandro Horga, Majdi Kara, Francesca Mattioli, Alice Goldenberg, Helen Griffin, Amelie Piton, Lindsay B. Henderson, Benyekhlef Kara, Ayca Dilruba Aslanger, Joost Raaphorst, Rolph Pfundt, Ruben Portier, Marwan Shinawi, Amelia Kirby, Katherine M. Christensen, Lu Wang, Rasim O. Rosti, Sohail A. Paracha, Muhammad T. Sarwar, Dagan Jenkins, Jawad Ahmed, Federico A. Santoni, Emmanuelle Ranza, Justyna Iwaszkiewicz, Cheryl Cytrynbaum, Rosanna Weksberg, Ingrid M. Wentzensen, Maria J. Guillen Sacoto, Yue Si, Aida Telegrafi, Marisa Andrews, Dustin Baldridge, Heinz Gabriel, Julia Mohr, Barbara Oehl-Jaschkowitz, Sylvain Debard, Bruno Senger, Frederic Fischer, Conny van Ravenwaaij, Annemarie J. M. Fock, Servi J. C. Stevens, Jurg Bahler, Amina Nasar, John F. Mantovani, Adnan Manzur, Anna Sarkozy, Desiree E. C. Smith, Gajja S. Salomons, Zubair M. Ahmed, Shaikh Riazuddin, Saima Riazuddin, Muhammad A. Usmani, Annette Seibt, Muhammad Ansar, Stylianos E. Antonarakis, John B. Vincent, Muhammad Ayub, Mona Grimmel, Anne Marie Jelsig, Tina Duelund Hjortshoj, Helena Gasdal Karstensen, Marybeth Hummel, Tobias B. Haack, Yalda Jamshidi, Felix Distelmaier, Rita Horvath, Joseph G. Gleeson, Hubert Becker, Jean-Louis Mandel, David A. Koolen, Henry Houlden
AMERICAN JOURNAL OF HUMAN GENETICS
(2020)
Article
Multidisciplinary Sciences
Nathaniel Yakobov, Frederic Fischer, Nassira Mahmoudi, Yusuke Saga, Christopher D. Grube, Herve Roy, Bruno Senger, Guillaume Grob, Shunsuke Tatematsu, Daisuke Yokokawa, Isabelle Mouyna, Jean-Paul Latge, Harushi Nakajima, Tetsuo Kushiro, Hubert D. Becker
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Biology
Gaetan Bader, Ludovic Enkler, Yuhei Araiso, Marine Hemmerle, Krystyna Binko, Emilia Baranowska, Johan-Owen De Craene, Julie Ruer-Laventie, Jean Pieters, Deborah Tribouillard-Tanvier, Bruno Senger, Jean-Paul di Rago, Sylvie Friant, Roza Kucharczyk, Hubert Dominique Becker
Article
Biology
Ludovic Enkler, Bruno Rinaldi, Johan Owen de Craene, Philippe Hammann, Osamu Nureki, Bruno Senger, Sylvie Friant, Hubert D. Becker
Summary: COPI-coated vesicles are involved in Golgi-to-ER and intra-Golgi trafficking pathways, mediating retrieval of ER resident proteins. In mammalian cells, proteins like GORAB, SCYL1, and SCYL3 regulate Golgi morphology and protein glycosylation in conjunction with the COPI machinery. The yeast protein Cex1, homologous to mammalian SCYL proteins, is a component of the COPI machinery and can potentially be used to study neurodegenerative disorders.
Article
Biochemistry & Molecular Biology
Nathaniel Yakobov, Nassira Mahmoudi, Guillaume Grob, Daisuke Yokokawa, Yusuke Saga, Tetsuo Kushiro, Danielle Worrell, Herve Roy, Hubert Schaller, Bruno Senger, Laurence Huck, Gisela Riera Gascon, Hubert D. Becker, Frederic Fischer
Summary: A wide range of bacteria and higher fungi possess virulence factors that are capable of rerouting aminoacyl-transfer RNAs away from protein synthesis to conjugate amino acids onto lipids. This study reports the discovery of a second type of fungal sterol-specific ATTs, namely, ergosteryl-3 beta-O-glycine (Erg-Gly) synthase (ErgS), expanding the repertoire of fungal lipids and suggesting the existence of a subfamily of lipid modifying ATTs.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Cell Biology
Ludovic Enkler, Viktoria Szentgyorgyi, Mirjam Pennauer, Cristina Prescianotto-Baschong, Isabelle Riezman, Aneta Wiesyk, Reut Ester Avraham, Martin Spiess, Einat Zalckvar, Roza Kucharczyk, Howard Riezman, Anne Spang
Summary: Enkler et al. demonstrate that the pool of Arf1 at lipid droplets plays a role in regulating mitochondrial ATP production by controlling fatty acid metabolism and acetyl-CoA transfer to mitochondria. This study provides insights into the cooperation between peroxisomes and mitochondria during fatty acid β-oxidation, highlighting the importance of Arf1 in fatty acid metabolism and energy production. The findings suggest that Arf1 integrates metabolism into energy production by regulating fatty acid storage and utilization, and possibly organelle contact sites.
NATURE CELL BIOLOGY
(2023)