Article
Biochemistry & Molecular Biology
Hyun Jun Woo, Ji Yeong Yang, Pyeongjae Lee, Jong-Bae Kim, Sa-Hyun Kim
Summary: The natural compound zerumbone extracted from Zingiber zerumbet Smith was found to inhibit the urease activity of H. pylori by forming dimers, trimers, or tetramers, suggesting its potential as a therapeutic agent for treating H. pylori infections in humans.
Article
Plant Sciences
Marina R. Wylie, Ian H. Windham, Faith C. Blum, Hannah Wu, D. Scott Merrell
Summary: Nimbolide, a compound found in neem tree extract, shows significant bactericidal activity against Helicobacter pylori, making it a potential future treatment for H. pylori infection.
JOURNAL OF ETHNOPHARMACOLOGY
(2022)
Article
Multidisciplinary Sciences
Jiashen Zhou, Lin Zhang, Liping Zeng, Lu Yu, Yuanyuan Duan, Siqi Shen, Jingyan Hu, Pan Zhang, Wenyan Song, Xiaoxue Ruan, Jing Jiang, Yinan Zhang, Lu Zhou, Jia Jia, Xudong Hang, Changlin Tian, Houwen Lin, Hong-Zhuan Chen, John E. Cronan, Hongkai Bi, Liang Zhang
Summary: The study presents the crystal structures of the essential UFA biosynthesis enzyme FabX in Helicobacter pylori, revealing a unique [4Fe-4S] cluster that potentiates FMN oxidation during dehydrogenase catalysis. This [4Fe-4S] cluster is crucial for unsaturated fatty acid synthesis and contributes to the pathogenic function of H. pylori in the corrosion of gastric mucosa.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Ji Yeong Yang, Jong-Bae Kim, Pyeongjae Lee, Sa-Hyun Kim
Summary: Evodiamine inhibits the growth of H. pylori by downregulating gene expressions related to replication and transcription machineries of the bacteria. Moreover, evodiamine also suppresses urease expression, leading to reduced translocation of CagA and VacA proteins into AGS cells. Additionally, evodiamine inhibits the activation of signaling proteins induced by H. pylori infection, contributing to reduction of IL-8 production in AGS cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Ling Wang, Wen-Qun Li, Fen Liu, Yuan-Jian Li, Jie Du
Summary: This study investigates the variations of xCT activity in the gastric mucosa of patients with Helicobacter pylori (Hp) infection. The results show that patients with Hp infection have reduced xCT expression and lower glutamate concentration in gastric juice. The study also discovers a high expression of microRNAs that negatively regulate xCT expression in Hp-positive patients. The findings suggest that reduced xCT activity may play an important role in gastric ulcers caused by Hp infection and the microRNA/xCT pathway could be a potential treatment target for Hp-infection-related ulcers.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Shiyi Wang, Yiwen Zhao, Shufen Mao, Jiang Zhu, Yangyang Zhan, Dongbo Cai, Xin Ma, Dong Wang, Shouwen Chen
Summary: Disulfide bonds in proteins significantly affect folding efficiency by limiting conformational space. Inefficient formation of disulfide bonds is the main factor limiting enzyme activity and stability. This study aimed to increase the activity of disulfide-bond-containing proteins by promoting disulfide bond formation in Bacillus licheniformis. By introducing glutamate decarboxylase GAD from Escherichia coli into B. licheniformis, and successively overexpressing disulfide isomerase and oxidoreductase from different sources, the catalytic efficiency of GAD was improved. The engineered B. licheniformis showed significantly increased GAD activity (from 10.4 U/mg to 80.0 U/mg) and demonstrated adaptability for other disulfide-bond-containing proteins, such as UDP-glucosyltransferase from Arabidopsis thaliana. This work demonstrates the importance of disulfide bond formation for protein activity in B. licheniformis and provides a promising platform for expressing disulfide-bond-containing proteins.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Food Science & Technology
Jingfei Hu, Xueqing Tian, Tong Wei, Hangjie Wu, Jing Lu, Mingsheng Lyu, Shujun Wang
Summary: The exopolysaccharide (EPS) extracted from Lactobacillus plajomi PW-7 showed antibacterial activity and antioxidant capacity against Helicobacter pylori. It has a potential application in developing EPS-based foods. The structural characteristics and biological activity of EPS play a key role in its potential applications.
Review
Biochemistry & Molecular Biology
Rosanna Capparelli, Domenico Iannelli
Summary: Epigenetics plays a role in regulating gene expression, cell differentiation, and response to environmental stimuli. Helicobacter pylori (Hp) uses DNA methylation to silence genes in human gastric mucosa, which is a primary cause of gastric cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, General & Internal
Peter Malfertheiner, M. Constanza Camargo, Emad El-Omar, Jyh-Ming Liou, Richard Peek, Christian Schulz, Stella I. Smith, Sebastian Suerbaum
Summary: Helicobacter pylori infection causes chronic gastritis and can lead to severe gastroduodenal pathologies, including peptic ulcer and gastric cancer. This Primer summarizes the epidemiology, pathophysiology, diagnosis and management of this infection, and discusses patient quality of life and open research questions.
NATURE REVIEWS DISEASE PRIMERS
(2023)
Article
Chemistry, Multidisciplinary
Doha H. Abou Baker, Heba S. Abbas
Summary: Recently, the synthesis of selenium nanoparticles (SeNPs) using plant extracts as a substitute for chemical synthesis has gained attention. In this study, researchers synthesized biogenic SeNPs using pomegranate peels aqueous extract (PPAE) and evaluated their potential against Helicobacter pylori and stomach cancer cells. The results showed that the SeNPs exhibited significant anti-H. pylori activity and dose-dependent inhibition of stomach cancer cell growth. These findings suggest that the biogenic SeNPs have promising therapeutic potential for treating H. pylori infection and stomach cancer.
ARABIAN JOURNAL OF CHEMISTRY
(2023)
Article
Chemistry, Medicinal
Marta Maslanka, Wojciech Tabor, Pawel Krzyzek, Agnieszka Grabowiecka, Lukasz Berlicki, Artur Mucha
Summary: Catechols have been identified as potent covalent inhibitors of ureases by modifying cysteine residues at the active site. We synthesized novel catecholic derivatives with carboxylate and phosphonic/phosphinic groups, which demonstrated expanded specific interactions. The compound 2-(3,4-dihydroxyphenyl)-3-phosphonopropionic acid (15) exhibited significant anti-urease potential with a Ki value of 2.36 μM and displayed antiureolytic effects in live Helicobacter pylori cells at a submicromolar concentration (IC50 = 0.75 μM). Molecular modeling revealed its binding to the active site through electrostatic and hydrogen bond interactions. These catecholic phosphonic acids exhibited specific antiureolytic activity without cytotoxicity to eukaryotic cells.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Microbiology
Tran Thi Dieu Thuy, Pei-Yun Kuo, Sha-Ma Lin, Cheng-Yen Kao
Summary: This study evaluated and compared the anti-Helicobacter pylori activity of three potential probiotic strains and found that SLC13 showed the highest adhesion ability and inhibitory activity. It also alleviated the inflammatory response induced by H. pylori infection.
Article
Multidisciplinary Sciences
Tomohiko Yasuda, Hyun Seok Lee, Su Youn Nam, Hiroto Katoh, Yuko Ishibashi, Somay Yamagata Murayama, Hidenori Matsui, Hiroki Masuda, Emiko Rimbara, Nobuyuki Sakurazawa, Hideyuki Suzuki, Hiroshi Yoshida, Yasuyuki Seto, Shumpei Ishikawa, Seong Woo Jeon, Masahiko Nakamura, Sachiyo Nomura
Summary: Genetic analysis and culturing techniques for gastric non-Helicobacter pylori Helicobacter (NHPH) are advancing, with NHPH reported to accompany certain gastric conditions. A study on Korean gastric cancer patients found a low percentage positive for NHPH, suggesting it may play a role in gastric cancer development, albeit with lower pathogenicity compared to H. pylori.
SCIENTIFIC REPORTS
(2022)
Article
Immunology
Li Tang, Bo Tang, Yuanyuan Lei, Min Yang, Sumin Wang, Shiping Hu, Zhuo Xie, Yaojiang Liu, Israel Vlodavsky, Shiming Yang
Summary: Chronic gastritis caused by H. pylori infection is associated with up-regulation of heparanase, which facilitates the colonization of H. pylori in the gastric mucosa and exacerbates gastritis. Heparanase sustains a vicious cycle of inflammation and tumor progression by activating macrophages and promoting cytokine release, potentially increasing the risk of gastric cancer. Inhibition of heparanase may be a potential therapeutic strategy for reducing the risk of gastritis and gastric cancer.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Food Science & Technology
Sebastian Diechler, Bianca E. Chichirau, Gernot Posselt, Dionyssios N. Sgouras, Silja Wessler
Summary: Research has shown that the diverse structure of CagA regulates B cell physiology, while B cell survival is independent of CagA.
Article
Biochemistry & Molecular Biology
Won-Su Yoon, Seung-Hyeon Seok, Hyung-Sik Won, Taehwan Cho, Sang Jae Lee, Min-Duk Seo
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2019)
Article
Multidisciplinary Sciences
Donghyeon Lee, Sangwon Baek, Jaehyun Park, Keondo Lee, Jangwoo Kim, Sang Jae Lee, Wan Kyun Chung, Jong-Lam Lee, Yunje Cho, Ki Hyun Nam
SCIENTIFIC REPORTS
(2019)
Article
Multidisciplinary Sciences
Si Hoon Park, Jaehyun Park, Sang Jae Lee, Woo Seok Yang, Sehan Park, Kyungdo Kim, Zee-Yong Park, Hyun Kyu Song
SCIENTIFIC REPORTS
(2019)
Article
Biochemistry & Molecular Biology
Dong-Gyun Kim, Sujin Cho, Kyu-Yeon Lee, Seung-Ho Cheon, Hye-Jin Yoon, Joo-Youn Lee, Dongyoon Kim, Kwang-Soo Shin, Choong-Hyun Koh, Ji Sung Koo, Yuri Choi, Hyung Ho Lee, Yu-Kyoung Oh, Yoo-Seong Jeong, Suk-Jae Chung, Moonkyu Baek, Kwan-Young Jung, Hyo Jin Lim, Hyoun Sook Kim, Sung Jean Park, Jeong-Yeon Lee, Sang Jae Lee, Bong-Jin Lee
Summary: NSDHL, a crucial enzyme in human cholesterol synthesis and regulator of EGFR, has been studied for its potential therapeutic applications. Two X-ray crystal structures of human NSDHL were reported, revealing coenzyme-binding details and a conformational change. A novel inhibitor for NSDHL was identified, showing suppressive activity towards EGFR, enhancing antitumor effects in EGFR-driven cancer cells. These findings may serve as a platform for developing therapeutic agents against NSDHL-related diseases.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
DongWon Park, Hye-Jin Yoon, Ki-Young Lee, Sung-Jean Park, Seung-Ho Cheon, Hyung Ho Lee, Sang Jae Lee, Bong-Jin Lee
Article
Chemistry, Multidisciplinary
Kyu-Yeon Lee, Seung-Ho Cheon, Dong-Gyun Kim, Sang Jae Lee, Bong-Jin Lee
Article
Chemistry, Multidisciplinary
Vivek Srinivas, Rahul Banerjee, Hugo Lebrette, Jason C. Jones, Oskar Aurelius, In-Sik Kim, Cindy C. Pham, Sheraz Gul, Kyle D. Sutherlin, Asmit Bhowmick, Juliane John, Esra Bozkurt, Thomas Fransson, Pierre Aller, Agata Butryn, Isabel Bogacz, Philipp Simon, Stephen Keable, Alexander Britz, Kensuke Tono, Kyung Sook Kim, Sang-Youn Park, Sang Jae Lee, Jaehyun Park, Roberto Alonso-Mori, Franklin D. Fuller, Alexander Batyuk, Aaron S. Brewster, Uwe Bergmann, Nicholas K. Sauter, Allen M. Orville, Vittal K. Yachandra, Junko Yano, John D. Lipscomb, Jan Kern, Martin Hogbom
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2020)
Article
Optics
Inhyuk Nam, Chang-Ki Min, Bonggi Oh, Gyujin Kim, Donghyun Na, Young Jin Suh, Haeryong Yang, Myung Hoon Cho, Changbum Kim, Min-Jae Kim, Chi Hyun Shim, Jun Ho Ko, Hoon Heo, Jaehyun Park, Jangwoo Kim, Sehan Park, Gisu Park, Seonghan Kim, Sae Hwan Chun, HyoJung Hyun, Jae Hyuk Lee, Kyung Sook Kim, Intae Eom, Seungyu Rah, Deming Shu, Kwang-Je Kim, Sergey Terentyev, Vladimir Blank, Yuri Shvyd'ko, Sang Jae Lee, Heung-Sik Kang
Summary: The hard X-ray self-seeded X-ray free-electron laser at the Pohang Accelerator Laboratory offers high peak brightness and stability, providing superior data quality compared to SASE mode.
Article
Biochemical Research Methods
Dong Gyun Kim, Kyu Yeon Lee, Sang Jae Lee, Seung Ho Cheon, Yuri Choi, Hyung Ho Lee, Hee Chul Ahn, Bong Jin Lee
Summary: The metallo-beta-lactamase fold is a common metal-binding domain found in bacteria and archaea. Crystal structures of SAV1707, a metalloenzyme from Staphylococcus aureus, and its complex with cAMP were reported, revealing different metal selectivities for phosphodiesterase and endonuclease activities. Functional studies confirmed the role of residue Phe511 in substrate recognition.
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
(2021)
Article
Genetics & Heredity
Youngim Kim, Sang Jae Lee, Changkon Park, Jasung Koo, Euiyoung Bae, Bong-Jin Lee, Jeong-Yong Suh
Summary: AcrIIC2 inhibits the nuclease activity of type II-C Cas9, forming heterogeneous high-order oligomers driven by electrostatic interactions between charged residues. Upon binding with AcrIIC2, the BH peptide of Cas9 undergoes a coil-to-helix transition, revealing a unique folding-upon-binding mechanism for Acr recognition.
Article
Biochemistry & Molecular Biology
Do-Hee Kim, Sung-Min Kang, Sung-Min Baek, Hye-Jin Yoon, Dong Man Jang, Hyoun Sook Kim, Sang Jae Lee, Bong-Jin Lee
Summary: Staphylococcus aureus, a pathogenic bacterium with global distribution, has been the focus of recent research on developing novel antibiotics based on its toxin-antitoxin systems. This study investigates the structure and function of the PemIK system in S. aureus, providing valuable information for the discovery and development of antibiotics that inhibit the direct binding between PemI and PemK.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Multidisciplinary Sciences
Stephen M. Keable, Adrian Koelsch, Philipp S. Simon, Medhanjali Dasgupta, Ruchira Chatterjee, Senthil Kumar Subramanian, Rana Hussein, Mohamed Ibrahim, In-Sik Kim, Isabel Bogacz, Hiroki Makita, Cindy C. Pham, Franklin D. Fuller, Sheraz Gul, Daniel Paley, Louise Lassalle, Kyle D. Sutherlin, Asmit Bhowmick, Nigel W. Moriarty, Iris D. Young, Johannes P. Blaschke, Casper de Lichtenberg, Petko Chernev, Mun Hon Cheah, Sehan Park, Gisu Park, Jangwoo Kim, Sang Jae Lee, Jaehyun Park, Kensuke Tono, Shigeki Owada, Mark S. Hunter, Alexander Batyuk, Roland Oggenfuss, Mathias Sander, Serhane Zerdane, Dmitry Ozerov, Karol Nass, Henrik Lemke, Roman Mankowsky, Aaron S. Brewster, Johannes Messinger, Nicholas K. Sauter, Vittal K. Yachandra, Junko Yano, Athina Zouni, Jan Kern
Summary: The structure of cyanobacterial PS I at room temperature revealed an expansion of the protein complex compared to cryogenic structures. Conformational differences were observed between the two branches in the reaction center, which may provide insights into the preferential directionality of electron transfer.
SCIENTIFIC REPORTS
(2021)
Article
Biochemical Research Methods
Dong-Gyun Kim, Inwha Baek, Yeon Lee, Hyerry Kim, Jin Young Kim, Geul Bang, Sunghwan Kim, Hye Jin Yoon, Byung Woo Han, Se Won Suh, Hyoun Sook Kim
Summary: The initiation of host tissue infection by Staphylococcus aureus relies on a family of adhesive proteins containing SDR domains. This study elucidates a novel mechanism by which SdgB and SdgA carry out glycosyl-transfer processes and highlights the importance of their structures and activities in drug development against staphylococcal infections.
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Keondo Lee, Donghyeon Lee, Sangwon Baek, Jihan Kim, Jaehyun Park, Sang Jae Lee, Sehan Park, Jangwoo Kim, Jong-Lam Lee, Wan Kyun Chung, Yunje Cho, Ki Hyun Nam
Summary: This study investigated the radiation damage caused by an XFEL to PDMS and PI materials, commonly used in sample delivery devices. The results showed different structural damages in PDMS and PI films, providing insights for developing applications of these materials in XFEL studies.
APPLIED SCIENCES-BASEL
(2022)
Article
Biochemistry & Molecular Biology
Hoyoung Kim, Taehyun Lim, Go Eun Ha, Jee-Young Lee, Jun-Woo Kim, Nienping Chang, Si Hyun Kim, Ki Hun Kim, Jaeick Lee, Yongju Cho, Byeong Wook Kim, Alva Abrahamsson, Sung Hwan Kim, Hyo-Ji Kim, Sehan Park, Sang Jae Lee, Jaehyun Park, Eunji Cheong, B. Moon Kim, Hyun-Soo Cho
Summary: This study utilizes X-ray free electron laser technology to determine the structural characteristics of the corticotropin-releasing hormone receptor 1 protein complexes and develops potential antidepressant antagonist compounds.
EXPERIMENTAL AND MOLECULAR MEDICINE
(2023)
