☆ 4.5 Article

Human Cdc25A phosphatase has a non-redundant function in G2 phase by activating Cyclin A-dependent kinases

FEBS LETTERS (2009)

Journal

FEBS LETTERS

Volume 583, Issue 4, Pages 841-847

Publisher

WILEY

DOI: 10.1016/j.febslet.2009.01.044

Keywords

Cell cycle; Cdc25A; Mitosis; Cyclin A; Cyclin B/Cdk1

Categories

Biochemistry & Molecular Biology Biophysics Cell Biology

Funding

Deutsche Krebshilfe [107058]
DKFZ Guest scientist and intramural programs
Gottfried Daimler und Karl Benz-Stiftung

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Abstract

Cdc25 phosphatases activate Cdk/Cyclin complexes by dephosphorylation and thus promote cell cycle progression. We observed that the peak activity of Cdc25A precedes the one of Cdc25B in prophase and the maximum of Cyclin/Cdk kinase activity. Furthermore, Cdc25A activates both Cdk1-2/Cyclin A and Cdk1/Cyclin B complexes while Cdc25B seems to be involved only in activation of Cdk1/Cyclin B. Concomitantly, repression of Cdc25A led to a decrease in Cyclin A-associated kinase activity and attenuated Cdk1 activation. Our results indicate that Cdc25A acts before Cdc25B - at least in cancer cells, and has non-redundant functions in late G2/early M-phase as a major regulator of Cyclin A/kinase complexes. (C) 2009 Published by Elsevier B. V. on behalf of the Federation of European Biochemical Societies.

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