Article
Biotechnology & Applied Microbiology
Guanglian Liao, Lu Chen, Yanqun He, Xishi Li, Zhengxin Lv, Shuyao Yi, Min Zhong, Chunhui Huang, Dongfeng Jia, Xueyan Qu, Xiaobiao Xu
Summary: This study revealed the metabolic characteristics and key enzymes of Ascorbic Acid (AsA) in kiwifruit, as well as identified candidate genes related to AsA biosynthesis, cycling, and degradation through transcriptome analysis. The research results indicate that the accumulation of AsA in fruit mainly depends on the L-galactose pathway.
Article
Agronomy
Yan Zhang, Kang Wang, Xiang Xiao, Shifeng Cao, Wei Chen, Zhenfeng Yang, Liyu Shi
Summary: 1-MCP treatment delays fruit senescence and maintains higher AsA and T-AsA content in postharvest kiwifruit. The transcription levels of genes involved in AsA metabolic pathway are regulated by 1-MCP treatment. The up-regulation of AsA biosynthesis and regeneration genes, along with the down-regulation of degradation genes, contribute to the increase in AsA level in treated kiwifruit. Additionally, the up-regulation of candidate bHLH transcription factors may also play a role in the beneficial effects of 1-MCP on AsA content.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2021)
Article
Nutrition & Dietetics
Zhenyu Luo, Jieru Zhang, Miaolian Xiang, Jiaoke Zeng, Jinyin Chen, Ming Chen
Summary: Exogenous melatonin can delay the softening and decrease of acidity in postharvest kiwifruit, inhibit the increase of soluble solids content, and maintain a higher content of ascorbic acid during storage. Moreover, it regulates the expression of key genes involved in ascorbic acid metabolism.
FRONTIERS IN NUTRITION
(2022)
Article
Nutrition & Dietetics
Kota Shiozawa, Misato Maeda, Hsin-Jung Ho, Tomoko Katsurai, Md Zakir Hossain Howlader, Kimiko Horiuchi, Yumi Sugita, Yusuke Ohsaki, Afifah Zahra Agista, Tomoko Goto, Michio Komai, Hitoshi Shirakawa
Summary: The study found that supplementation of biotin increased testosterone levels in mice and testis-derived cells. This effect was mediated by the activation of adenylate cyclase and protein kinase A. These findings suggest that biotin may have the potential to improve age-related male syndromes associated with declining testosterone production.
Article
Biochemistry & Molecular Biology
Masayo Omura, Yukie Takabatake, Eugene Lempert, Sigi Benjamin-Hong, Charlotte D'Hulst, Paul Feinstein
Summary: The molecular basis of odor perception in humans remains elusive due to the challenges in studying odorant receptors (ORs) outside their natural environment. This study presents a method using cilia isolated from mouse olfactory sensory neurons to measure odor-specific responses with higher sensitivity. This approach offers a sensitive and scalable platform for ex vivo odor screening.
Article
Biochemistry & Molecular Biology
Lorena Rodriguez-Paez, Charmina Aguirre-Alvarado, Norma Oviedo, Veronica Alcantara-Farfan, Edgar E. Lara-Ramirez, Guadalupe Elizabeth Jimenez-Gutierrez, Joaquin Cordero-Martinez
Summary: This study investigated the influence of polyamines on sperm channels and found that polyamines interacted with sodium and potassium channels, potentially affecting the membrane potential of sperm. Additionally, alkalization of pHi during capacitation suggested that increased levels of cAMP may activate NHEs, which is supported by in silico analysis of interactions with polyamines.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Evelyn Silva de Aguiar, Abigailde Nascimento Dias, Raquel Mendes Sousa, Thais Andrade Germano, Renato Oliveira de Sousa, Rafael De Souza Miranda, Jose Helio Costa, Clesivan Pereira dos Santos
Summary: This study investigates the expression of genes involved in ascorbate biosynthesis in pepper through genome- and transcriptome-wide analysis. The results suggest that the L-galactose pathway plays a primary role in ascorbate biosynthesis, while genes from alternative pathways have a secondary role under specific conditions. The findings provide promising candidate genes for future experiments aimed at increasing ascorbate content in peppers and other crops.
Article
Horticulture
Yuanxiu Lin, Bing Zhao, Honglan Tang, Lijuan Cheng, Yunting Zhang, Yan Wang, Jinming Fan, Mengyao Li, Qing Chen, Ya Luo, Xiaorong Wang, Haoru Tang, Yong Zhang
Summary: This study investigated the differences in AsA content and potential mechanisms between two cultivars of hardy kiwifruit. The results showed that GalLDH is a key enzyme for AsA biosynthesis in hardy kiwifruit, while MDHAR is more efficient than DHAR in AsA regeneration. Moreover, AO and APX play important roles in AsA oxidation. This study provides a reference for further genetic improvement and selection of high AsA content materials in cultivation.
SCIENTIA HORTICULTURAE
(2022)
Article
Biophysics
Tongle Liu, Yuan Rao, Wenfeng Zhou, Wei Zhuang, Lei Ge, Rijia Lin, Wenxia Zhang, Jinglan Wu, Chenjie Zhu, Hanjie Ying
Summary: By modifying graphene oxide with PDA/PEI and utilizing the affinity between metal ions and AC's His-tags, directional immobilization of AC was successfully achieved. The modified GO increases amino density for affinity group decoration and adjusts the interaction between AC and the support. This method can improve enzyme activity and immobilization efficiency.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Biochemistry & Molecular Biology
Misato Ikoma, Yusuke Nakasone, Masahide Terazima
Summary: The photochemical reaction of photoactivated adenylate cyclase from cyanobacterium mPAC was investigated using time-resolved transient grating method, revealing a rapid equilibrium between dimer and tetramer upon light exposure. The PAS and AC domains, as well as the N- and C-terminal helices of the LOV domain, were found to play important roles in inducing conformational change and tetramer formation. The reaction scheme of mPAC involves a light-induced conformation shift, primarily influenced by the angle changes of the coiled-coil structures in certain helices.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
(2021)
Article
Cardiac & Cardiovascular Systems
Yohann Rautureau, Marianne Berlatie, Daniel Rivas, Kurunradeth Uy, Alexandre Blanchette, Geraldine Miquel, Marie-Eve Higgins, Melanie Mecteau, Audrey Nault, Louis Villeneuve, Veronique Lavoie, Gabriel Theberge-Julien, Genevieve Brand, Line Lapointe, Maxime Denis, Camille Rosa, Annik Fortier, Lucie Blondeau, Marie-Claude Guertin, Marie-Pierre Dube, Eric Thorin, Jonathan Ledoux, David Rhainds, Eric Rheaume, Jean-Claude Tardif
Summary: ADCY9 gene plays a role in determining atherosclerotic outcomes in patients and in mice it has been shown to potentiate endothelial function and inhibit atherogenesis. The inactivation of Adcy9 leads to increased cAMP accumulation in response to certain activators and enhances endothelial-dependent vasodilation. Additionally, ADCY9 can regulate endothelial permeability and actin cytoskeleton in response to thrombin, affecting the development of atherosclerosis.
CARDIOVASCULAR RESEARCH
(2023)
Article
Agriculture, Dairy & Animal Science
Fawzia A. Hassan, Azhar G. Shalaby, Nabila Elsaid M. Elkassas, Shawky A. El-Medany, Ahmed Hamdi Rabie, Khalid Mahrose, Ayman Abd El-Aziz, Samar Bassiony
Summary: Orange peel and its extract are rich sources of phenols and vitamin C that can be used as powerful antioxidants and antibacterial agents. This study investigated the effects of dietary supplementation with orange peel powder and orange peel extract on the growth performance, blood biochemicals, gene expression, and antioxidant status of growing rabbits under hot conditions. The results showed that supplementation with orange peel powder and orange peel extract improved rabbit performance and reduced lipid and bacterial levels.
ANIMAL BIOTECHNOLOGY
(2022)
Article
Microbiology
Shu-Fan Yu, Zhan-Bin Sun, Shi-Dong Li, Ya-Feng Hu, Qing Ren, Jia-Liang Xu, Han-Jian Song, Man-Hong Sun
Summary: In this study, the role of the cAMP signaling pathway in C. rosea mycoparasitism was explored through gene knockout and complementation. It was found that crac deletion influenced the growth rate, colony morphology, and stress tolerance of C. rosea. The mycoparasitic effects and biocontrol capacity of C. rosea were attenuated in crac knockout mutants.
Article
Physiology
K. A. S. N. Shanaka, Sumi Jung, N. D. Janson, J. R. P. Jayasingha, K. P. Madushani, Myoung-Jin Kim, Jehee Lee
Summary: In this study, the active GULO gene from cloudy catshark was cloned into zebrafish to reestablish the AA biosynthesis pathway, resulting in increased AA production, improved growth, and decreased reactive oxygen species production. The successful integration and expression of the sGULO construct in zebrafish were confirmed, providing evidence for the beneficial effects of reestablished AA synthesis in zebrafish.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Multidisciplinary Sciences
Lasse K. Markussen, Elizabeth A. Rondini, Olivia Sveidahl Johansen, Jesper G. S. Madsen, Elahu G. Sustarsic, Ann-Britt Marcher, Jacob B. Hansen, Zachary Gerhart-Hines, James G. Granneman, Susanne Mandrup
Summary: This study uncovers the transcriptional network controlled by lipolysis in brown adipocytes and shows that lipolysis is required for the activation of the thermogenic gene program by beta-adrenergic signals. PPARs are identified as key mediators of lipolysis-induced activation of lipid metabolism and thermogenic genes. Additionally, lipolysis also activates the unfolded protein response and regulates the core circadian transcriptional machinery independently of PPARs.
NATURE COMMUNICATIONS
(2022)
