Strategies for discovery and improvement of enzyme function: state of the art and opportunities

Enzyme Promiscuity: A Mechanistic and Evolutionary Perspective

(2010) Olga Khersonsky and Dan S. Tawfik   Annual Review of Biochemistry

A novel metagenome-derived β-galactosidase: gene cloning, overexpression, purification and characterization

(2010) Kui Wang et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

A nitrilase from a metagenomic library acts regioselectively on aliphatic dinitriles

(2010) Sally Bayer et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Biochemical characterization of two novel β-glucosidase genes by metagenome expression cloning

(2010) Chengjian Jiang et al.   BIORESOURCE TECHNOLOGY

The α/β-Hydrolase Fold 3DM Database (ABHDB) as a Tool for Protein Engineering

(2010) Robert Kourist et al.   CHEMBIOCHEM

Access to enantiopure aromatic epoxides and diols using epoxide hydrolases derived from total biofilter DNA

(2010) Michael Kotik et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Screening for hydrocarbon biodegraders in a metagenomic clone library derived from Brazilian petroleum reservoirs

(2010) Suzan Pantaroto de Vasconcellos et al.   ORGANIC GEOCHEMISTRY

Guidelines and Cost Analysis for Catalyst Production in Biocatalytic Processes

(2010) Pär Tufvesson et al.   ORGANIC PROCESS RESEARCH & DEVELOPMENT

Biocatalytic Asymmetric Synthesis of Chiral Amines from Ketones Applied to Sitagliptin Manufacture

(2010) C. K. Savile et al.   SCIENCE

Computational Design of an Enzyme Catalyst for a Stereoselective Bimolecular Diels-Alder Reaction

(2010) J. B. Siegel et al.   SCIENCE

Chemical sulfation of small molecules—advances and challenges

(2010) Rami A. Al-Horani et al.   TETRAHEDRON

Organic solvent-tolerant enzymes

(2009) Noriyuki Doukyu et al.   BIOCHEMICAL ENGINEERING JOURNAL

Evolution of Enzymatic Activities in the Enolase Superfamily: Stereochemically Distinct Mechanisms in Two Families ofcis,cis-Muconate Lactonizing Enzymes†‡

(2009) Ayano Sakai et al.   BIOCHEMISTRY

High Yield Synthesis of 12-Aminolauric Acid by “Enzymatic Transcrystallization” of ω-Laurolactam Using ω-Laurolactam Hydrolase fromAcidovoraxsp. T31

(2009) Yasuhisa FUKUTA et al.   BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY

Directed enzyme evolution: climbing fitness peaks one amino acid at a time

(2009) Cara A Tracewell et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Metagenomics reveals diversity and abundance ofmeta-cleavage pathways in microbial communities from soil highly contaminated with jet fuel under air-sparging bioremediation

(2009) Maria V. Brennerova et al.   ENVIRONMENTAL MICROBIOLOGY

Biochemical characterization of a metagenome-derived decarboxylase

(2009) Chengjian Jiang et al.   ENZYME AND MICROBIAL TECHNOLOGY

Investigative mining of sequence data for novel enzymes: A case study with nitrilases

(2009) Jennifer L. Seffernick et al.   JOURNAL OF BIOTECHNOLOGY

Directed evolution drives the next generation of biocatalysts

(2009) Nicholas J Turner   Nature Chemical Biology

Enantioselective kinetic resolution of phenylalkyl carboxylic acids using metagenome-derived esterases

(2009) Elena Fernández-Álvaro et al.   Microbial Biotechnology

Nature versus nurture in two highly enantioselective esterases fromBacillus cereusandThermoanaerobacter tengcongensis

(2009) Stephan Grosse et al.   Microbial Biotechnology

Asymmetric Synthesis of Optically Pure Pharmacologically Relevant Amines Employing ω-Transaminases

(2008) Dominik Koszelewski et al.   ADVANCED SYNTHESIS & CATALYSIS

Cloning and characterization of a new cold-active lipase from a deep-sea sediment metagenome

(2008) Jeong Ho Jeon et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

The Screening, Characterization, and Use of ω-Laurolactam Hydrolase: A New Enzymatic Synthesis of 12-Aminolauric Acid

(2008) Yasuhisa ASANO et al.   BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY

Directed evolution of transketolase substrate specificity towards an aliphatic aldehyde

(2008) E HIBBERT et al.   JOURNAL OF BIOTECHNOLOGY

Predicting enzyme behavior in nonconventional media: correlating nitrilase function with solvent properties

(2008) Praveen Kaul et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Advances in Recovery of Novel Biocatalysts from Metagenomes

(2008) Helen L. Steele et al.   JOURNAL OF MOLECULAR MICROBIOLOGY AND BIOTECHNOLOGY

A genomic search approach to identify esterases in Propionibacterium freudenreichii involved in the formation of flavour in Emmental cheese

(2008) Julien Dherbécourt et al.   Microbial Cell Factories

Kemp elimination catalysts by computational enzyme design

(2008) Daniela Röthlisberger et al.   NATURE