Article
Multidisciplinary Sciences
Hui Yang, Yurui Dong, Ying Bian, Nuo Xu, Yuwei Wu, Fan Yang, Yinping Du, Tao Qin, Sujuan Chen, Daxin Peng, Xiufan Liu
Summary: The PB2 subunit of influenza virus polymerase inhibits the host immune response by blocking interferon induction. It reduces cellular sensitivity to interferons and suppresses the activation of STAT1/STAT2 and ISGs. It targets mammalian JAK1 for ubiquitination and degradation. Mutations on PB2 increase the ability to degrade mammalian JAK1 and enhance viral replication and pathogenicity in mammalian cells and mouse lung tissues. This study provides insights into the evasion strategy employed by influenza virus to evade host antiviral immunity.
NATURE COMMUNICATIONS
(2022)
Article
Immunology
Gang Lu, Feiyan Zheng, Jiajun Ou, Xin Yin, Shoujun Li
Summary: In this study, the feline RNA PolI promoter was identified and found to have higher transcriptional activity in feline cells. The equine influenza virus showed higher polymerase activity compared to human and canine influenza viruses. Additionally, the feline myxovirus resistance protein 1 and baloxavir were found to inhibit influenza virus polymerase activity.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Chemistry, Medicinal
WooChan Kim, Jung-Ah Kang, Minji Park, Pyeong-Hwa Jeong, Yoon Jun Kim, Yuri Cho, Sung-Gyoo Park, Yong-Chul Kim
Summary: Novel potent pyrimidine derivatives have been synthesized as core assembly modulators for the treatment of chronic hepatitis B virus (HBV) infection. These derivatives showed potent inhibitory effects in in vitro assays and significantly decreased serum HBV DNA levels in a human liver-chimeric uPA/SCID mouse model, particularly when combined with tenofovir.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Microbiology
Yiping Wang, Yanwu Wei, Hongli Wu, Li Feng, Liping Huang
Summary: This study identified the key domains that mediate the interaction between PRV UL30 and UL42, providing potential targets for designing a novel intervention strategy against PRV infection. The findings also support the concept that herpesvirus DNA polymerase subunits utilize their extreme carboxy-terminal domains to interact with their accessory subunits, suggesting the opportunity of designing novel antiviral agents against herpesvirus infection through disruption of the polymerase subunit interactions.
VETERINARY MICROBIOLOGY
(2022)
Article
Virology
Hana Veler, Haitian Fan, Jeremy R. Keown, Jane Sharps, Marjorie Fournier, Jonathan M. Grimes, Ervin Fodor
Summary: This study characterizes the interaction between the viral polymerase and Rab11a and identifies the important domains involved in this interaction. The findings provide insights into the cytoplasmic transport of vRNPs and suggest a potential target site for the development of influenza antiviral drugs.
JOURNAL OF VIROLOGY
(2022)
Article
Chemistry, Medicinal
Avishak Sarker, Zichen Gu, Lu Mao, Yongzhuang Ge, Duoduo Hou, Jieyu Fang, Zhanyong Wei, Zhenya Wang
Summary: Influenza is a century-old disease that poses challenges to humans due to its mutating nature, seasonal epidemics, and occasional pandemics. The limited effectiveness of strain-specific vaccines and the growing drug resistance to anti-influenza drugs call for the development of novel drugs with broad reactivity, higher bioavailability, easier administration, and fewer side effects.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Materials Science, Multidisciplinary
Marlen Kruse, Christin Moeser, David M. Smith, Hanna Mueller-Landau, Ulrich Rant, Ralph Hoelzel, Frank F. Bier
Summary: Electrically controllable DNA nanolevers were used to study the binding interaction between Influenza A virus and PeB peptide. The study showed that the virus specifically binds to immobilized peptides, and can be used to characterize rate constants of virus-receptor interactions.
ADVANCED MATERIALS TECHNOLOGIES
(2022)
Article
Biochemistry & Molecular Biology
Steven F. Baker, Helene Meistermann, Manuel Tzouros, Aaron Baker, Sabrina Golling, Juliane Siebourg Polster, Mitchell P. Ledwith, Anthony Gitter, Angelique Augustin, Hassan Javanbakht, Andrew Mehle
Summary: The host range of influenza virus is limited by successful interactions between the virus and cellular proteins. The study identifies a cellular protein called cMECR that interacts with the viral polymerase and suppresses viral replication by blocking assembly of viral ribonucleoprotein complexes.
Article
Microbiology
Chao Ma, Yuhan Li, Yanan Zong, Tony Velkov, Chenxi Wang, Xinyu Yang, Ming Zhang, Zhimin Jiang, Haoran Sun, Qi Tong, Honglei Sun, Juan Pu, Munir Iqbal, Jinhua Liu, Chongshan Dai, Yipeng Sun
Summary: The influenza A virus (IAV) can activate p21 through a p53-independent pathway. p21 directly binds to the viral polymerase acidic protein, limiting IAV polymerase activity by disrupting the formation of the ribonucleoprotein complex. p21 activation promotes IRF3 activation by blocking the degradation of HO-1, leading to the activation of the type I interferon pathway.
Article
Multidisciplinary Sciences
Franziska Guenl, Tim Krischuns, Julian A. Schreiber, Lea Henschel, Marius Wahrenburg, Hannes C. A. Drexler, Sebastian A. Leidel, Vlad Cojocaru, Guiscard Seebohm, Alexander Mellmann, Martin Schwemmle, Stephan Ludwig, Linda Brunotte
Summary: The replication of influenza A virus relies on ubiquitination of the viral polymerase derived from host cells. This study reveals that site-specific ubiquitination of PB1-K578 is acquired during infection and regulates the dimerization of polymerase and the binding of NP. Mass spectrometry analysis has identified 59 modified lysines across the three subunits of the viral polymerase, which affect mRNA transcription, vRNA replication, and the generation of recombinant viruses via non-proteolytic mechanisms.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Liuke Sun, Huihui Kong, Mengmeng Yu, Zhenyu Zhang, Haili Zhang, Lei Na, Yuxing Qu, Yuan Zhang, Hualan Chen, Xiaojun Wang
Summary: Species differences in ANP32A/B restrict avian influenza virus in mammalian cells. Adaptive mutations, such as PB2-E627K, are required for efficient replication of avian influenza viruses in mammals. We found that NS2 protein promotes avian vRNP assembly and enhances interactions with mammalian ANP32A/B, overcoming the restriction. Disrupting the conserved SUMO-interacting motif (SIM) in NS2 impairs virus replication and pathogenicity in mammals, indicating NS2 as a cofactor in the adaptation process of avian influenza virus to mammals.
Article
Pharmacology & Pharmacy
Michael G. Ison, Frederick G. Hayden, Alan J. Hay, Larisa Gubareva, Elena A. Govorkova, Emi Takashita, Jennifer L. McKimm-Breschkin
Summary: This article summarized the current knowledge on the clinical impact of resistance to polymerase inhibitors, surveillance methods, and laboratory evaluation methods. Limitations and gaps in current knowledge were highlighted, with suggestions for future research including additional clinical studies of influenza antiviral drug combinations. Lessons learned from influenza resistance monitoring may also be useful for establishing future drug susceptibility surveillance and testing for SARS-CoV-2.
ANTIVIRAL RESEARCH
(2021)
Article
Virology
Emi Takashita, Shin Murakami, Yoko Matsuzaki, Seiichiro Fujisaki, Hiroko Morita, Shiho Nagata, Misa Katayama, Katsumi Mizuta, Hidekazu Nishimura, Shinji Watanabe, Taisuke Horimoto, Hideki Hasegawa
Summary: To minimize public health risks, monitoring antiviral susceptibilities of influenza viruses is crucial. This study tested the susceptibility of influenza C and D viruses to RNA polymerase inhibitors and found that all tested viruses were susceptible without any associated amino acid substitutions. The combined use of focus reduction assay and genotypic assay has proven valuable for monitoring antiviral susceptibilities of influenza C and D viruses.
Review
Cell Biology
Kate Dicker, Aino I. Jarvelin, Manuel Garcia-Moreno, Alfredo Castello
Summary: RNA viruses rely on cellular RNA-binding proteins to facilitate replication and spread, with a pool of RBPs typically incorporated into viral particles. These RBPs play crucial roles in viral particle formation and infectivity, suggesting broader implications for host RBPs in virus infection than previously thought.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Saisai Guo, Xiaobo Lei, Yan Chang, Jianyuan Zhao, Jing Wang, Xiaojing Dong, Qian Liu, Zixiong Zhang, Lidan Wang, Dongrong Yi, Ling Ma, Quanjie Li, Yongxin Zhang, Jiwei Ding, Chen Liang, Xiaoyu Li, Fei Guo, Jianwei Wang, Shan Cen
Summary: Research has identified CDK2 as a key host factor in SARS-CoV-2 RNA replication, facilitating viral RNA synthesis, and CDK2 inhibitors have shown potential in impairing viral replication and infection.
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2022)
Article
Biochemistry & Molecular Biology
Katharina Roltgen, Sandra C. A. Nielsen, Oscar Silva, Sheren F. Younes, Maxim Zaslavsky, Cristina Costales, Fan Yang, Oliver F. Wirz, Daniel Solis, Ramona A. Hoh, Aihui Wang, Prabhu S. Arunachalam, Deana Colburg, Shuchun Zhao, Emily Haraguchi, Alexandra S. Lee, Mihir M. Shah, Monali Manohar, Iris Chang, Fei Gao, Vamsee Mallajosyula, Chunfeng Li, James Liu, Massa J. Shoura, Sayantani B. Sindher, Ella Parsons, Naranjargal J. Dashdorj, Naranbaatar D. Dashdorj, Robert Monroe, Geidy E. Serrano, Thomas G. Beach, R. Sharon Chinthrajah, Gregory W. Charville, James L. Wilbur, Jacob N. Wohlstadter, Mark M. Davis, Bali Pulendran, Megan L. Troxell, George B. Sigal, Yasodha Natkunam, Benjamin A. Pinsky, Kari C. Nadeau, Scott D. Boyd
Summary: During the SARS-CoV-2 pandemic, different vaccines have been used globally. This study compares the antibodies generated by mRNA vaccines, infection, and other types of vaccines. It shows that mRNA vaccines result in a better antibody breadth against viral variants compared to infection. Infection leads to variant-specific antibodies, while mRNA vaccination imprints responses towards the original virus strain. mRNA vaccines also stimulate robust germinal centers in lymph nodes, enhancing the immune response.
Article
Biochemistry & Molecular Biology
Dakai Liu, George D. Rodriguez, Hang-Yu Zhou, Ye-Xiao Cheng, Xiaofeng Li, Wenwen Tang, Nishant Prasad, Chun-Cheng Chen, Vishnu Singh, Eric Konadu, Keither K. James, Maria F. Bahamon, Yvonne Chen, Sorana Segal-Maurer, Aiping Wu, William Harry Rodgers
Summary: This study, conducted in the epicenter of the COVID-19 pandemic, tested 193,054 specimens for SARS-CoV-2 RNA using mRT-PCR and found 17,196 positive results. Whole genome amplification (WGA) and Next Generation Sequencing (NGS) were performed on 9516 isolates to investigate virus molecular evolution and epidemiology. The results showed a continuation of genetic divergence in viral genomes, with independent mutations accumulating over time in primer and probe regions. Some of these mutations were correlated with changes in the detection pattern of viral targets for mRT-PCR. The study highlights the importance of continuous genetic divergence in gene amplification-based assays, the value of the mRT-PCR detection pattern in complementing the clinical diagnosis of reinfection, and the potential of WGA and NGS in identifying mutation hotspots to optimize PCR-based gene amplification assays.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Chunfeng Li, Audrey Lee, Lilit Grigoryan, Prabhu S. Arunachalam, Madeleine K. D. Scott, Meera Trisal, Florian Wimmers, Mrinmoy Sanyal, Payton A. Weidenbacher, Yupeng Feng, Julia Z. Adamska, Erika Valore, Yanli Wang, Rohit Verma, Noah Reis, Diane Dunham, Ruth O'Hara, Helen Park, Wei Luo, Alexander D. Gitlin, Peter Kim, Purvesh Khatri, Kari C. Nadeau, Bali Pulendran
Summary: The BNT162b2 mRNA vaccine stimulates potent antibody and antigen-specific T cell responses, as well as enhanced innate responses after secondary immunization. Circulating IFN-gamma is mainly produced by natural killer cells and CD8(+) T cells in the draining lymph nodes. The CD8(+) T cell response is dependent on type I interferon-dependent MDA5 signaling.
Article
Virology
Cheng-Yang Ji, Na Han, Ye-Xiao Cheng, Jingzhe Shang, Shenghui Weng, Rong Yang, Hang-Yu Zhou, Aiping Wu
Summary: This article proposes a computational strategy to detect potentially adaptive mutations from the phylogenetic trajectory and identifies important sites that have experienced paraFix mutations in the dynamic evolution of SARS-CoV-2. These findings provide valuable clues for disease control and prevention.
Article
Oncology
Lishu Chen, Chao Zhou, Qi Chen, Jingzhe Shang, Zhaodan Liu, Yan Guo, Chunfeng Li, HongJiang Wang, Qing Ye, XiaoFeng Li, Shulong Zu, Fangye Li, Qing Xia, Tao Zhou, Ailing Li, Chenhui Wang, Yun Chen, Aiping Wu, Chengfeng Qin, Jianghong Man
Summary: Zika virus treatment can enhance immune cell infiltration and activation in glioblastoma and inhibit tumor growth. Additionally, Zika virus can activate the interferon signaling pathway in glioblastoma cells. This therapy can improve the sensitivity of glioblastoma to immune checkpoint blockade.
MOLECULAR THERAPY-ONCOLYTICS
(2022)
Article
Microbiology
Shenghui Weng, Hangyu Zhou, Chengyang Ji, Liang Li, Na Han, Rong Yang, Jingzhe Shang, Aiping Wu
Summary: This study identified structural variations in SARS-CoV-2 and highlighted the potential impact of deletions on virus transmission and vaccine development.
MICROBIOLOGY SPECTRUM
(2022)
Article
Virology
Lulan Wang, Hang-Yu Zhou, Jia-Ying Li, Ye-Xiao Cheng, Shilei Zhang, Saba Aliyari, Aiping Wu, Genhong Cheng
Summary: The study provides evidence of intervariant and intravariant recombination of rapidly evolving SARS-CoV-2 genomes, suggesting potential for more infectious, immune-escaping, and disease-causing Omicron and Delta-Omicron variants.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Article
Immunology
Jingzhe Shang, Chunfeng Li, Zhujia Jin, Shulong Zu, Songjie Chen, Junlan Chen, Ziyi Chen, Hua Tang, Cheng-Feng Qin, Qing Ye, Aiping Wu
Summary: The study investigates the immune response to Zika virus infection and its impact on neural tissue damage. The results suggest that Zika viruses with high pathogenicity can induce sustained activation of the immune system, leading to nerve tissue damage.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Virology
Yexiao Cheng, Chengyang Ji, Na Han, Jiaying Li, Lin Xu, Ziyi Chen, Rong Yang, Hang-Yu Zhou, Aiping Wu
Summary: A subsampling method named covSampler was developed for SARS-CoV-2 genome sequences, taking into account both their spatiotemporal distribution and genetic diversity. The method is efficient and stable, with customizable options for users. This work provides an easy-to-use tool and webserver for subsampling SARS-CoV-2 genome sequences.
Article
Biochemistry & Molecular Biology
Shulong Zu, Chunfeng Li, Lili Li, Yong-Qiang Deng, Xiang Chen, Dan Luo, Qing Ye, Yi-Jiao Huang, Xiao-Feng Li, Rong-Rong Zhang, Nina Sun, Xianqi Zhang, Saba R. Aliyari, Karin Nielsen-Saines, Jae U. Jung, Heng Yang, Cheng-Feng Qin, Genhong Cheng
Summary: TRIM22, an ISG, plays a crucial role in host defense against flaviviruses by binding and degrading NS1 and NS3 proteins.
CELL AND BIOSCIENCE
(2022)
Article
Biochemical Research Methods
Chengyang Ji, Na Han, Yexiao Cheng, Jingzhe Shang, Shenghui Weng, Rong Yang, Hang-Yu Zhou, Aiping Wu
Summary: sitePath is a computational tool based on R that automates the identification of polymorphism clades, inference of fixed and parallel mutations, and generation of high-quality figures.
BMC BIOINFORMATICS
(2022)
Article
Biochemical Research Methods
Zena Cai, Ping Fu, Ye Qiu, Aiping Wu, Gaihua Zhang, Yirong Wang, Taijiao Jiang, Xing-Yi Ge, Haizhen Zhu, Yousong Peng
Summary: vsRNAfinder is a de novo method for identifying high-confidence vsRNAs from sRNA-Seq data, which outperforms widely used methods in identifying viral miRNAs and shows similar performance in animal and plant sRNAs identification. It greatly facilitates the effective identification of vsRNAs from sRNA-Seq data.
BRIEFINGS IN BIOINFORMATICS
(2022)
Review
Virology
Yexiao Cheng, Chengyang Ji, Hang-Yu Zhou, Heng Zheng, Aiping Wu
Summary: The growing SARS-CoV-2 genomic data provide valuable information for researchers and public health officials concerning the transmission and evolution of the virus. Various web resources have been developed to aid in the analysis of these data, including storage, collation, analysis, and visualization. This review summarizes the web resources used for SARS-CoV-2 genomic epidemiology, covering data management, annotation, analysis, and variant tracking. Challenges and future expectations for these resources are discussed, emphasizing the importance of continued development and improvement for effectively tracking the spread and understanding the evolution of the virus.
Article
Multidisciplinary Sciences
Hao Wu, Xing-Yao Huang, Meng-Xu Sun, Yue Wang, Hang-Yu Zhou, Ying Tian, Beijia He, Kai Li, De-Yu Li, Ai-Ping Wu, Hongmei Wang, Cheng-Feng Qin
Summary: This study investigated the infectivity and pathological effects of Zika virus (ZIKV) on placental trophoblast progenitor cells in early human embryos. The researchers found that human trophoblast stem cells (hTSCs) were susceptible to ZIKV infection, but resistance increased with cell differentiation. ZIKV infection disrupted the structure of mature hTSC-organoids and inhibited syncytialization. The researchers also suggested that ZIKV infection may lead to a preeclampsia phenotype.
NATURE COMMUNICATIONS
(2023)
Article
Virology
Jie Sheng, Lili Li, Xueying Lv, Meiling Gao, Ziyi Chen, Zhuo Zhou, Jingfeng Wang, Aiping Wu, Taijiao Jiang
Summary: Through manually curating published studies, we established a comprehensive network of 3,591 human proteins interacting with 31 SARS-CoV-2 viral proteins, and identified four key host factors involved in SARS-CoV-2 infection. Among them, SERPINE1 was found to facilitate SARS-CoV-2 replication and alleviate endoplasmic reticulum stress induced by ORF8 protein through interaction. These findings highlight the importance of systematic integration analysis in understanding SARS-CoV-2-human interactions.
