Article
Cell Biology
Laura A. Sherer, Naomi Courtemanche
Summary: The assembly of actin-based structures relies on both polymerization and crosslinking, which play interdependent roles in shaping the architectures of actin bundles. In this study, we investigated the impact of filament elongation on the formation of actin bundles by the crosslinking protein fascin. Our findings demonstrate that filament length affects the rate of bundle assembly, with longer filaments promoting bundle coalescence into interconnected networks. However, the connections formed between elongating bundles are short-lived and often followed by filament breakage. These results highlight the importance of maintaining precise control over filament length during the assembly of specialized actin structures.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Microbiology
Elizabeth A. Rucks
Summary: Type III secretion systems (T3SS) are utilized by Gram-negative pathogens to deliver effector proteins into target eukaryotic cells for manipulating cell functions and enhancing pathogenesis. This review will discuss the history, biochemical characterization, and function of chlamydial T3SS, as well as the use of heterologous/surrogate models for studying it. The review will also cover the history of chlamydial effectors and recent advances in the field.
MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS
(2023)
Article
Multidisciplinary Sciences
Eduardo Soto, Jorge E. Galan, Marfa Lara-Tejero
Summary: In this study, an extensive in vivo cross-linking strategy aided by structure modeling was used to investigate the sorting platform complex of type III secretion systems. The assembly process of this bacterial structure was mapped using identified cross-links as signatures for pairwise intersubunit interactions and systematic genetic deletions. Insights generated by this study could be used for the rational development of antivirulence strategies against medically important bacterial pathogens.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Plant Sciences
Irene Jimenez-Guerrero, Monica Sonawane, Noam Eckshtain-Levi, Za Khai Tuang, Gustavo Mateus da Silva, Francisco Perez-Montano, Meirav Leibman-Markus, Rupali Gupta, Lianet Noda-Garcia, Maya Bar, Saul Burdman
Summary: This study characterized an effector called AopW1 in the pathogen Acidovorax citrulli and showed its variation in sequence plays a role in host adaptation. It also provided new insights into the HopW1 family of bacterial effectors and demonstrated the involvement of the endosome-associated protein EHD1 in response to biotic stress.
Article
Multidisciplinary Sciences
Stephan Wimmi, Alexander Balinovic, Hannah Jeckel, Lisa Selinger, Dimitrios Lampaki, Emma Eisemann, Ina Meuskens, Dirk Linke, Knut Drescher, Ulrike Endesfelder, Andreas Diepold
Summary: Current research shows that in gastrointestinal pathogens, the external pH plays a crucial role in regulating the assembly of T3SS cytosolic components, preventing T3SS activity in the stomach and facilitating its reactivation in the intestine.
NATURE COMMUNICATIONS
(2021)
Article
Plant Sciences
Naama Wagner, Michael Alburquerque, Noa Ecker, Edo Dotan, Ben Zerah, Michelle Mendonca Pena, Neha Potnis, Tal Pupko
Summary: In this study, novel machine-learning algorithms were developed to characterize the secretion signal of Type III effectors in both plant and animal pathogens. A benchmark dataset was curated and the results showed that the novel approach had substantially better classification accuracy compared to previous methodologies. The study also revealed that plant and animal pathogen effectors may have different secretion signals.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Julie Ming Liang, Lisa Ann Burdette, Han Teng Wong, Danielle Tullman-Ercek
Summary: Protein production using bacteria is costly and inefficient. Researchers have engineered a bacterial secretion system to improve the production process by directly secreting soluble proteins into the culture media. They explored different activation methods and created strains capable of efficiently producing heterologous proteins. They also discovered a genetic relationship between T3SS regulators, hilE and hilD, further enhancing the industrial competitiveness of protein production strains.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Emel Sen-Kilic, Annalisa B. Huckaby, F. Heath Damron, Mariette Barbier
Summary: This study used RNA-seq analysis to investigate the transcriptome response of type II pneumocytes during P. aeruginosa infection, revealing that P. aeruginosa modulates the proinflammatory response of epithelial cells through T3SS and T6SS, promoting infection progression.
Article
Microbiology
Miguel Diaz-Guerrero, Meztlli O. Gaytan, Eduardo Soto, Norma Espinosa, Elizabeth Garcia-Gomez, Arely Marcos-Vilchis, Angel Andrade, Bertha Gonzalez-Pedrajo
Summary: The type III secretion system (T3SS) is a complex molecular device that enables pathogenic bacteria to transport effector proteins directly into host cells. The CesL/SepL/SepD complex regulates the switch from translocator to effector secretion in enteropathogenic Escherichia coli, ensuring proper secretion and stability of the components involved. CesL also interacts with the cytoplasmic domains of export gate components, contributing to the regulation of substrate secretion.
Review
Biochemistry & Molecular Biology
Amit Meir, Kevin Mace, Yogesh Vegunta, Sunanda M. Williams, Gabriel Waksman
Summary: This review provides an update on recent advances in substrate recruitment and delivery by recruitment platforms associated with Type III, IV, and VI secretion systems in gram-negative bacteria.
TRENDS IN MICROBIOLOGY
(2023)
Article
Immunology
Wei-Jie Song, Jun Gao, Ji-Wei Huang, Yuan Liu, Zhi Long, Le-Ye He
Summary: By detecting the composition and function of microorganisms in expressed prostatic secretion (EPS) of patients with chronic prostatitis (CP) and healthy people, this study explored whether type III prostatitis is related to bacterial infection. The results showed that there were many microorganisms in EPS in both CP patients and healthy populations, but the relative abundance of specific bacteria in CP patients, including Pseudomonas, Haemophilus, Sneathia, Allobaculum, and Enterococcus, was significantly different. Metagenomics sequencing also revealed that different bacteria in the type III prostatitis group had certain biological functions.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Chemistry, Inorganic & Nuclear
Bappaditya Goswami, Manas Khatua, Subhas Samanta
Summary: In this study, four Co(II) complexes were synthesized and characterized using various techniques. Their catalytic activity in styrene polymerization was evaluated, with complex 3 showing the highest efficiency among the azo-aromatic complexes, while the imine-based complex 4 exhibited lower activity.
DALTON TRANSACTIONS
(2022)
Article
Microbiology
Kevin Munoz Navarrete, Ladislav Bumba, Tatyana Prudnikova, Ivana Malcova, Tania Romero Allsop, Peter Sebo, Jana Kamanova
Summary: The study reveals that BopN is a gatekeeper of the Bordetella T3SS, responsible for regulating the secretion and translocation of the BteA effector into host cells. It is similar to gatekeepers that control access to the T3SS channel from the bacterial cytoplasm. Deletion of the bopN gene in B. bronchiseptica increased secretion of BteA, but decreased the translocation of BteA into host cell cytoplasm.
MICROBIOLOGY SPECTRUM
(2023)
Article
Multidisciplinary Sciences
Brianna Steiert, Carolina M. Icardi, Robert Faris, Paige N. McCaslin, Parker Smith, Aloysius J. Klingelhutz, Peter M. Yau, Mary M. Weber
Summary: The centrosome is crucial for mitotic spindle assembly, chromosome segregation, and cell division. Infection by Chlamydia trachomatis (C.t.) leads to blocked cytokinesis, supernumerary centrosomes, and multipolar spindles, but the mechanisms behind these cellular abnormalities remain largely unknown. This study shows that the secreted effector protein CteG binds to CETN2 and is necessary for infection-induced centrosome amplification. These findings provide insights into C.t.-induced cellular abnormalities and its potential contribution to cancer risk.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Multidisciplinary Sciences
Marc-Andre Leblanc, Morgan R. Fink, Thomas T. Perkins, Marcelo C. Sousa
Summary: The study shows that effector proteins from certain bacteria can be injected into host cells through a type III secretion system. These proteins need to be partially unfolded to pass through the narrow channel. Mechanical unfolding of the proteins revealed that they exhibit low force and high mechanical compliance, facilitating efficient secretion.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Chiaki Ota, Masamichi Ikeguchi, Akiyoshi Tanaka, Daizo Hamada
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2016)
Article
Microbiology
Bochao Wang, Mitsuhiro Nishimura, Huamin Tang, Akiko Kawabata, Nora F. Mahmoud, Zahra Khanlari, Daizo Hamada, Hiroki Tsuruta, Yasuko Mori
Article
Biochemistry & Molecular Biology
Marina Nawata, Hirotaka Tsutsumi, Yuta Kobayashi, Satoru Unzai, Shouhei Mine, Tsutomu Nakamura, Koichi Uegaki, Hironari Kamikubo, Mikio Kataoka, Daizo Hamada
Article
Immunology
Kimiko Kuroki, Kazuhiro Mio, Ami Takahashi, Haruki Matsubara, Yoshiyuki Kasai, Sachie Manaka, Masahide Kikkawa, Daizo Hamada, Chikara Sato, Katsumi Maenaka
JOURNAL OF IMMUNOLOGY
(2017)
Article
Biochemistry & Molecular Biology
Kenji Sasahara, Kenichi Morigaki, Takashi Okazaki, Daizo Hamada
Article
Biochemistry & Molecular Biology
Yuta Kobayashi, Hirotaka Tsutsumi, Tetsuyuki Abe, Kyohei Ikeda, Yuki Tashiro, Satoru Unzai, Hironari Kamikubo, Mikio Kataoka, Hidekazu Hiroaki, Daizo Hamada
Article
Developmental Biology
Naokazu Inoue, Daizo Hamada, Hironari Kamikubo, Kunio Hirata, Mikio Kataoka, Masaki Yamamoto, Masahito Ikawa, Masaru Okabe, Yoshihisa Hagihara
Article
Biochemistry & Molecular Biology
Naoko Iwaya, Kohei Akiyama, Natsuko Goda, Takeshi Tenno, Yoshie Fujiwara, Daizo Hamada, Teikichi Ikura, Masahiro Shirakawa, Hidekazu Hiroaki
Article
Biochemistry & Molecular Biology
Hideyuki Takahashi, Naoko Adachi, Toshihiko Shirafuji, Sally Danno, Takehiko Ueyama, Michele Vendruscolo, Anton N. Shuvaev, Takuya Sugimoto, Takahiro Seki, Daizo Hamada, Kazuhiro Irie, Hirokazu Hirai, Norio Sakai, Naoaki Saito
HUMAN MOLECULAR GENETICS
(2015)
Article
Biochemistry & Molecular Biology
Naoko Iwaya, Hirotoshi Takasu, Natsuko Goda, Masahiro Shirakawa, Toshiki Tanaka, Daizo Hamada, Hidekazu Hiroaki
JOURNAL OF BIOCHEMISTRY
(2013)
Article
Biochemistry & Molecular Biology
Aoi Nakazono, Naoko Adachi, Hideyuki Takahashi, Takahiro Seki, Daizo Hamada, Takehiko Ueyama, Norio Sakai, Naoaki Saito
JOURNAL OF BIOLOGICAL CHEMISTRY
(2018)
Article
Biochemistry & Molecular Biology
Kyoko Hiragami-Hamada, Kaori Shinmyozu, Daizo Hamada, Yoshiro Tatsu, Koichi Uegaki, Shinsuke Fujiwara, Jun-ichi Nakayama
MOLECULAR AND CELLULAR BIOLOGY
(2011)
Article
Multidisciplinary Sciences
Mitsuhide Hamaguchi, Hironari Kamikubo, Kayo N. Suzuki, Yoshihisa Hagihara, Itaru Yanagihara, Ikuhiro Sakata, Mikio Kataoka, Daizo Hamada
Article
Multidisciplinary Sciences
Y. Matsushita, H. Sekiguchi, C. Jae Wong, M. Nishijima, K. Ikezaki, D. Hamada, Y. Goto, Y. C. Sasaki
SCIENTIFIC REPORTS
(2017)
Article
Biochemistry & Molecular Biology
Hidekazu Hiroaki, Kaori Satomura, Natsuko Goda, Yukako Nakakura, Minami Hiranuma, Takeshi Tenno, Daizo Hamada, Takahisa Ikegami