期刊
FEBS JOURNAL
卷 275, 期 24, 页码 6260-6267出版社
WILEY
DOI: 10.1111/j.1742-4658.2008.06750.x
关键词
actin branching; actin bundling; co-sedimentation; pyrene-actin polymerization; type III secretion system
资金
- Japanese Society for the Promotion of Science
- National Center for Child Health and Development, Japan
EspB is a multifunctional protein associated with the type III secretion system of enterohaemorrhagic Escherichia coli, and interacts with various biomolecules including alpha-catenin in the host cell. The binding of EspB to alpha-catenin is thought be involved in actin reorganization during bacterial infection, although the precise mechanism of this phenomenon is still unclear. Recent research shows that dimerization of alpha-catenin dissociates it from E-cadherin/beta-catenin/alpha-catenin complexes, and that the dimer suppresses Arp2/3-mediated actin branching or polymerization. These results inspired us to evaluate the effect of EspB on the functions of alpha-catenin. Based on a series of in vitro biochemical approaches, including pull-down, co-sedimentation and pyrene-actin polymerization assays combined with transmission electron microscopy, we conclude that EspB promotes all the functions of dimeric alpha-catenin described above. These results clarified the molecular basis of reorganization of actin filaments during infection with enterohaemorrhagic Escherichia coli.
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