Article
Cell & Tissue Engineering
David Castillo-Azofeifa, Tomas Wald, Efren A. Reyes, Aaron Gallagher, Julia Schanin, Stephanie Vlachos, Nathalie Lamarche-Vane, Carolyn Bomidi, Sarah Blutt, Mary K. Estes, Todd Nystul, Ophir D. Klein
Summary: A central factor in maintaining tissue integrity is how stem cells respond to changes in niche signals. In the gut, intestinal stem cells (ISCs) rely on Wnt ligands for self-renewal and proliferation. This study shows that Discs large 1 (Dlg1) is necessary for ISC survival in the presence of increased Wnt signaling, even though it is not essential for polarity and cellular turnover during intestinal homeostasis. DLG1 regulates the cellular response to increased canonical Wnt ligands by controlling the transcription of Arhgap31, which deactivates CDC42, a component of the non-canonical Wnt pathway. These findings highlight the importance of the DLG1-ARHGAP31-CDC42 axis in the ISC response to increased Wnt signaling in the niche.
Review
Biochemistry & Molecular Biology
Qiyun Xiao, Johannes Werner, Nachiyappan Venkatachalam, Kim E. Boonekamp, Matthias P. Ebert, Tianzuo Zhan
Summary: Targeting cancer hallmarks is crucial for improving anti-cancer treatment, but cross-talk between signaling pathways often leads to resistance. This article provides an overview of the molecular interactions between the p53 and Wnt pathways in cancer, including complex feedback loops and reciprocal transactivation, as well as the mutational landscape of genes associated with these pathways.
Article
Biochemistry & Molecular Biology
Jinchao Li, Ying Tang, Ting-Chun Lin, Huawei Zeng, Joel B. Mason, Zhenhua Liu
Summary: Strong evidence suggests an association between body fatness and the development of colorectal cancer (CRC), but there is still limited understanding of the causality between obesity and CRC. This study investigates the role of TNF-alpha in obesity-associated CRC development. The research shows that the deletion of TNF-alpha reduces inflammation and Wnt signaling, thereby attenuating obesity-induced intestinal tumorigenesis. Targeting TNF-alpha signaling may be a potential strategy for reducing the risk of CRC associated with obesity.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2023)
Article
Medicine, Research & Experimental
Sanne M. van Neerven, Wouter L. Smit, Milou S. van Driel, Vaishali Kakkar, Nina E. de Groot, Lisanne E. Nijman, Clara C. Elbers, Nicolas Leveille, Jarom Heijmans, Louis Vermeulen
Summary: The majority of colorectal cancers have early mutations in the tumor suppressor gene APC, leading to oncogenic activation of the Wnt pathway and increased cell proliferation and mRNA translation. A study discovered that the protein chaperone HSP25 plays a crucial role in driving tumor initiation in colorectal cancer. Inhibition of HSP25 reduces the development of premalignant adenomas, suggesting its potential as a target for chemopreventive strategies in high-risk individuals.
EMBO MOLECULAR MEDICINE
(2022)
Article
Oncology
Takahiro Hamoya, Gen Fujii, Yosuke Iizumi, Takumi Narita, Masami Komiya, Yui Matsuzawa, Kohei Miki, Tadashi Kondo, Shinji Kishimoto, Kenji Watanabe, Keiji Wakabayashi, Toshiyuki Sakai, Jiro Toshima, Michihiro Mutoh
Summary: Artesunate inhibits intestinal tumorigenesis by reducing the nuclear translocation of TCF/LEF transcription factors, thereby suppressing Wnt signaling.
Article
Oncology
Michael Bordonaro
Summary: Deregulated Wnt signaling is responsible for most cases of colorectal cancer. Dietary fiber protects against colorectal cancer, likely through butyrate-mediated hyperactivation of Wnt signaling. Receptor-mediated Wnt signaling is associated with poor prognosis, while oncogenic Wnt signaling is associated with relatively good prognosis.
Article
Nanoscience & Nanotechnology
Xiao-Min Liu, Wen-Ting Zhu, Meng-Lei Jia, Yu-Ting Li, Ye Hong, Zhong-Qiu Liu, Peng-Ke Yan
Summary: The study demonstrated that the combination of Rapa/Lps and 5-FU effectively suppressed colorectal tumor formation by inhibiting angiogenesis and proliferation.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2022)
Review
Oncology
Kuang He, Wen-Juan Gan
Summary: The Wnt/beta-catenin signaling pathway is closely associated with the carcinogenesis and progression of colorectal cancer (CRC), playing a crucial role in cancer-related processes. Hyperactivation of the pathway is observed in almost all CRC cases and contributes to cancer stem cell propagation, angiogenesis, epithelial-mesenchymal transition (EMT), chemoresistance, and metastasis. This review will discuss the involvement of the Wnt/beta-catenin signaling pathway in CRC and potential therapeutic approaches.
CANCER MANAGEMENT AND RESEARCH
(2023)
Article
Medicine, Research & Experimental
Aysenur Gok, Aynur Isik, Sinem Bakir, Sarp Uzun, Gunes Guner, Ozge Ozcan, Baris Cerci, Ilyas Onbasilar, Aytekin Akyol
Summary: This study examined the effects of reduced Bdnf levels in an Apc mutant intestinal/colonic tumor mouse model. The results showed that Bdnf mutant and Apc/Bdnf double mutant mice had a significant weight gain compared to controls. The small intestine polyp count decreased slightly in Apc/Bdnf double mutant mice, while the colon polyp count increased significantly.
Review
Cell Biology
Ishita S. Hiremath, Arul Goel, Sudha Warrier, Alan Prem Kumar, Gautam Sethi, Manoj Garg
Summary: The Wnt/β-catenin signaling pathway is crucial for various physiological processes and implicated in the development of several cancers. Different regulators of the pathway play essential roles in cancer development.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Oncology
Margherita Pergolizzi, Laura Bizzozero, Federica Maione, Elena Maldi, Claudio Isella, Marco Macagno, Elisa Mariella, Alberto Bardelli, Enzo Medico, Caterina Marchio, Guido Serini, Federica Di Nicolantonio, Federico Bussolino, Marco Arese
Summary: This study revealed that NLGN1 is expressed in human colorectal tumors and promotes CRC cell migration and metastasis. Mechanistically, NLGN1 promotes the localization of APC to the cell membrane, stimulates beta-catenin translocation to the nucleus, and induces an EMT phenotype in CRC cell lines. This novel finding suggests NLGN1 as a potential therapeutic target for CRC.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2022)
Article
Biotechnology & Applied Microbiology
Jinpeng Yao, Yuanhua Cai, Zhixiang Chen, Xuechun Wang, Xiaolin Lai, Lili Pan, Yang Li, Shaoyuan Wang
Summary: This study revealed a new role for DHX15 in zebrafish intestinal development. We found intestinal defects, decreased proliferation, and increased apoptosis in dhx15 knockout zebrafish. RNA genome wide analysis and qRT-PCR analysis showed down-regulation of the Wnt signaling pathway in dhx15 knockout zebrafish. Therefore, we concluded that dhx15 regulates zebrafish intestinal development through the Wnt signaling pathway. This study provides new insights into the role of dhx15 in intestinal development beyond its well-characterized role in intestinal immunity.
Article
Nutrition & Dietetics
Louisa Filipe Rosa, Patricia P. Petersen, Lisa F. Goertz, Iris Stolzer, Valentina Kaden-Volynets, Claudia Guenther, Stephan C. Bischoff
Summary: Deficiencies in vitamin A and D are associated with immune modulation and impaired intestinal barrier. However, the underlying mechanisms are not well understood. In this study, mice deficient in vitamin A or D were fed specific diets for 12 weeks, and their gut barrier function, antimicrobial peptide defense, and regulatory pathways were assessed. The results showed that deficiencies in vitamin A and D led to decreased levels of antimicrobial peptides and alterations in related gene expression. Additionally, an increase in pro-inflammatory markers and beta-defensins was observed in the colon of deficient mice. Cell culture experiments confirmed that vitamins A and D regulate antimicrobial peptide expression through the Jak/STAT5 signaling pathway.
Article
Oncology
Yang Zhou, Jiang Xu, Haichang Luo, Xiangjing Meng, Ming Chen, Di Zhu
Summary: Abnormal activation of the Wnt/beta-catenin signaling pathway is closely related to tumorigenesis and immune surveillance, leading to increased resistance to immunotherapy.
Article
Biochemistry & Molecular Biology
Seong-Ho Lee, Hee-Seop Lee, Jihye Lee, Darshika Amarakoon, Zhiyuan Lou, Leela E. Noronha, Thomas J. Herald, Ramasamy Perumal, Dmitriy Smolensky
Summary: High-phenolic sorghum bran extracts have been found to inhibit proliferation and induce apoptosis in colorectal cancer cell lines. They can repress NF-kappa B and PI3K/AKT pathways while activating AMPK and autophagy, which significantly suppress tumor formation in colorectal cancer mouse models.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Agriculture, Multidisciplinary
Xiang Wen, Hui Zhao, Liwen Wang, Liang Wang, Gang Du, Wenqiang Guan, Jianfu Liu, Xiaocang Cao, Xiaohua Jiang, Jingrui Tian, Meiyan Wang, Chi-Tang Ho, Shiming Li
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2020)
Article
Biotechnology & Applied Microbiology
Bai Liang, Gao Mengxue, Cheng Xiaoming, Kang Guangbo, Cao Xiaocang, Huang He
MICROBIAL CELL FACTORIES
(2020)
Article
Medicine, Research & Experimental
Liyun Duan, Haoyan Huang, Xiaotong Zhao, Manqian Zhou, Shang Chen, Chen Wang, Zhibo Han, Zhong-Chao Han, Zhikun Guo, Zongjin Li, Xiaocang Cao
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
(2020)
Review
Immunology
Tianming Zhao, Lihong Mao, Zihan Yu, Yangyang Hui, Hongjuan Feng, Xiaoyu Wang, Lin Lin, Xiaofei Fan, Xin Chen, Bangmao Wang, Xiaocang Cao, Chao Sun
Summary: Bicyclol, a novel chemical drug derived from Schisandra chinensis, shows therapeutic potential in various liver conditions with a wide range of pharmacological properties. Pharmacokinetic studies indicate its safety and lack of drug interactions, making it a potential candidate for liver diseases worldwide. Further research is needed to understand its molecular mechanisms for increased potency in clinical use.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Letter
Gastroenterology & Hepatology
Xiaocang Cao, Haibin Yuan, Jie Liang, He Huang
INFLAMMATORY BOWEL DISEASES
(2022)
Review
Immunology
Jiewen Wang, Guangbo Kang, Haibin Yuan, Xiaocang Cao, He Huang, Ario de Marco
Summary: Recombinant antibodies like nanobodies are proving to be a valuable alternative to conventional monoclonal antibodies in clinical applications, with unique features that expand biotherapeutic options and alter disease treatment paradigms.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Microbiology
Lina Wang, Xiaoming Cheng, Liang Bai, Mengxue Gao, Guangbo Kang, Xiaocang Cao, He Huang
Summary: This study demonstrated that continuous consumption of engineered butyrate-producing bacteria had a significant inhibitory effect on obesity induced by a high-fat diet. The bacteria not only improved glucose and lipid metabolism but also regulated gut microbe composition. This research provided a new strategy for the effective and convenient treatment of obesity in the long term.
MICROBIOLOGY SPECTRUM
(2022)
Review
Immunology
Xiaoli Wang, Jingwen Zhao, Yuanhang Feng, Zelin Feng, Yulin Ye, Limin Liu, Guangbo Kang, Xiaocang Cao
Summary: The intestinal microbiome plays a crucial role in human health and disease, and microbiota transplantation is considered as a potential therapy for chronic diseases such as inflammatory bowel disease. Transplanting genetically diverse gut microbiota with metabolic flexibility and functional redundancy can effectively improve gut health. Natural fecal microbiota transplantation or washing microbiota transplantation has shown positive effects in alleviating colitis and improving intestinal dysbiosis. However, the complex and unclear composition of the flora limits its wider application.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Gastroenterology & Hepatology
Xiaocang Cao, Ang Dong, Guangbo Kang, Xiaoli Wang, Liyun Duan, Huixing Hou, Tianming Zhao, Shuang Wu, Xinjuan Liu, He Huang, Rongling Wu
Summary: This study integrates different theories to infer quasi-dynamic microbial networks from static abundance data, revealing various characteristics of microbial interactions and demonstrating their dynamic adaptability across space.
Review
Medicine, Research & Experimental
Zelin Feng, Guangbo Kang, Jiewen Wang, Xingjie Gao, Xiaoli Wang, Yulin Ye, Limin Liu, Jingwen Zhao, Xinjuan Liu, He Huang, Xiaocang Cao
Summary: Emerging biologics and small-molecule drugs have changed the treatment landscape of inflammatory bowel disease (IBD), but dual-targeted therapy (DTT) shows promise in overcoming treatment limitations. However, limited data on efficacy, safety, and mechanisms of DTT for Crohn's disease and ulcerative colitis exist. This review summarizes current data on novel targeted drugs for refractory IBD, categorizes therapeutic agents based on targets, and proposes mechanisms of DTT to aid clinical decision-making and future drug development.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Multidisciplinary Sciences
Juan Du, Junlei Zhang, Lin Wang, Xun Wang, Yaxing Zhao, Jiaoying Lu, Tingmin Fan, Meng Niu, Jie Zhang, Fei Cheng, Jun Li, Qi Zhu, Daoqiang Zhang, Hao Pei, Guang Li, Xingguang Liang, He Huang, Xiaocang Cao, Xinjuan Liu, Wei Shao, Jianpeng Sheng
Summary: By using imaging mass cytometry and single-cell RNA sequencing, researchers characterized the single-cell landscape of the human colon ecosystem in ulcerative colitis. They found tissue topological changes and a disappearance reaction of tissue-resident macrophages in the ulcerative colitis region. The study also revealed different reactive oxygen species vulnerability between inflammatory macrophages and resident macrophages, as well as a cytokine production network formed with T and B cells.
NATURE COMMUNICATIONS
(2023)
Article
Gastroenterology & Hepatology
Rirong Chen, Li Li, Kang Chao, Mengzhi Hong, Qian Cao, Lingna Ye, Gaoshi Zhou, Xiaoli Fang, Huili Guo, Xiaocang Cao, Xiaoqi Ye, Zhirong Zeng, Minhu Chen, Shenghong Zhang
THERAPEUTIC ADVANCES IN GASTROENTEROLOGY
(2020)
Article
Cell & Tissue Engineering
Huixing Hou, Leisheng Zhang, Liyun Duan, Yuanyuan Liu, Zhongchao Han, Zongjin Li, Xiaocang Cao
STEM CELL REVIEWS AND REPORTS
(2020)
Article
Medicine, Research & Experimental
Xiaocang Cao, Liyun Duan, Huixing Hou, Yue Liu, Shang Chen, Shuaiqiang Zhang, Yuanyuan Liu, Chen Wang, Xin Qi, Na Liu, Zhibo Han, Dekui Zhang, Zhong-Chao Han, Zhikun Guo, Qiang Zhao, Zongji Li
Article
Medicine, Research & Experimental
Shuaiqiang Zhang, Yuanyuan Liu, Xin Zhang, Dashuai Zhu, Xin Qi, Xiaocang Cao, Yihu Fang, Yongzhe Che, Zhong-Chao Han, Zuo-Xiang He, Zhibo Han, Zongjin Li