Article
Medicine, General & Internal
Arabella S. Stuart, Robert H. Shaw, Xinxue Liu, Melanie Greenland, Parvinder K. Aley, Nick J. Andrews, J. C. Cameron, Sue Charlton, Elizabeth A. Clutterbuck, Andrea M. Collins, Tom Darton, Tanya Dinesh, Christopher J. A. Duncan, Anna England, Saul N. Faust, Daniela M. Ferreira, Adam Finn, Anna L. Goodman, Christopher A. Green, Bassam Hallis, Paul T. Heath, Helen Hill, Bryn M. Horsington, Teresa Lambe, Rajeka Lazarus, Vincenzo Libri, Patrick J. Lillie, Yama F. Mujadidi, Ruth Payne, Emma L. Plested, Samuel Provstgaard-Morys, Maheshi N. Ramasamy, Mary Ramsay, Robert C. Read, Hannah Robinson, Gavin R. Screaton, Nisha Singh, David P. J. Turner, Paul J. Turner, Iason Vichos, Rachel White, Jonathan S. Nguyen-Van-Tam, Matthew D. Snape
Summary: The study investigated the mixed use of different COVID-19 vaccines within the same schedule, showing that in certain conditions, heterologous vaccination can achieve similar immunogenicity to homologous vaccination, facilitating rapid global vaccine deployment.
Article
Plant Sciences
Emmanuel Margolin, Georgia Schafer, Joel D. Allen, Sophette Gers, Jeremy Woodward, Andrew D. Sutherland, Melissa Blumenthal, Ann Meyers, Megan L. Shaw, Wolfgang Preiser, Richard Strasser, Max Crispin, Anna-Lise Williamson, Edward P. Rybicki, Ros Chapman
Summary: By applying an integrated host and glyco-engineering approach, a model antigen of the emerging virus SARS-CoV-2 was successfully produced in Nicotiana benthamiana. The protein produced in plants had a similar structure but differed in glycosylation compared to the protein produced in mammalian cells. Animals immunized with the plant-derived antigen showed reduced viral loads, lower lung pathology, and protection from weight loss after challenge, although the protection was not as potent as that induced by the mammalian cell-culture-derived antigen.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Immunology
Shridhar Bale, Lifei Yang, Mehrdad Alirezaei, Richard Wilson, Takayuki Ota, Esmeralda D. Doyle, Christopher A. Cottrell, Javier Guenaga, Karen Tran, Wenjuan Li, Leonidas Stamatatos, David Nemazee, Andrew B. Ward, Richard T. Wyatt
Summary: Fusion of the molecular adjuvant C3d to the Env trimers improves B-cell germinal center formation and antibody responses. In mice, this fusion enhances germinal center formation, magnitude of Env-specific binding antibodies, and antibody avidity.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Oncology
Sapna P. Patel, Gina R. Petroni, Jason Roszik, Walter C. Olson, Nolan A. Wages, Kimberly A. Chianese-Bullock, Mark Smolkin, Nikole Varhegyi, Elizabeth Gaughan, Kelly T. Smith, Kathleen Haden, Emily H. Hall, Sacha Gnjatic, Patrick Hwu, Craig L. Slingluff
Summary: This study evaluated the safety and immunogenicity of a novel long peptide vaccine with different adjuvants, showing that the LPV7 vaccine induced certain immune responses under various adjuvant strategies, but did not significantly enhance T cell responses compared to vaccines using short peptides.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2021)
Article
Immunology
Ana Carolina dos Reis Albuquerque Cajaraville, Mariana Pierre de Barros Gomes, Tamiris Azamor, Renata Carvalho Pereira, Patricia Cristina da Costa Neves, Paula Mello De Luca, Sheila Maria Barbosa de Lima, Luciane Pinto Gaspar, Elena Caride, Marcos da Silva Freire, Marco Alberto Medeiros
Summary: The attenuated yellow fever (YF) vaccine is highly effective but can cause rare fatal adverse events and has contraindications. Therefore, developing a non-live vaccine is important.
Article
Biochemical Research Methods
Katherine F. Warfel, Asher Williams, Derek A. Wong, Sarah E. Sobol, Primit Desai, Jie Li, Yung-Fu Chang, Matthew P. DeLisa, Ashty S. Karim, Michael C. Jewett
Summary: In this study, maltodextrin is identified as an additive to cell-free reactions that can increase thermostability and serve as a low-cost energy substrate. The optimized formulations of conjugate vaccines demonstrate storage stability at different temperatures and generate bactericidal antibodies against enterotoxigenic Escherichia coli (ETEC) O78 O-polysaccharide. This low-cost, thermostable cell-free glycoprotein synthesis system has the potential to revolutionize medicine biosynthesis and distribution by bypassing cold-chain requirements.
ACS SYNTHETIC BIOLOGY
(2023)
Article
Immunology
Saman Riaz, Hans Steinsland, Mette Thorsing, Ann Z. Andersen, Anders Boysen, Kurt Hanevik
Summary: The study found that during ETEC infection, most volunteers had IgA antibody responses targeting glycosylated YghJ epitopes, while gut IgA responses mainly targeted non-glycosylated epitopes, which is important for vaccine development efforts.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Oncology
Marit M. Melssen, Caroline T. Fisher, Craig L. Slingluff, Cornelis J. M. Melief
Summary: Water-in-oil emulsion incomplete Freund's adjuvant (IFA) has been widely used in vaccines, and the new generation Montanide adjuvants have been developed to reduce toxicity. Peptide vaccines in Montanide ISA-51 or ISA-720 can induce high antibody titers and durable effector T cell responses. CD4(+) T cell help and peptide affinity are important for an efficient T cell response. Additional inclusion of Toll-like receptor ligand can enhance the immune response.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2022)
Article
Agriculture, Multidisciplinary
Changli Yang, Fengyun Tian, Jie Ma, Mei Chen, Xingxing Shi, Dingli Chen, Youshudi Xie, Xingrong Zhou, Zhi Zhou, Xinlong Dai, Tao Xia, Liping Gao
Summary: This study identified 180 glycosyltransferase genes that may be involved in the biosynthesis of glycosylated secondary metabolites in tea plants. Among them, CsUGT74Y1 was found to be highly expressed in tea tender shoots and induced under stress conditions. In vitro experiments showed that CsUGT74Y1 catalyzed the glycosylation of various compounds. Additionally, Arabidopsis plants overexpressing CsUGT74Y1 exhibited enhanced growth and accumulation of specific glycosides.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Emmanuel Margolin, Joel D. Allen, Matthew Verbeek, Ros Chapman, Ann Meyers, Michiel van Diepen, Phindile Ximba, Thopisang Motlou, Penny L. Moore, Jeremy Woodward, Richard Strasser, Max Crispin, Anna-Lise Williamson, Edward P. Rybicki
Summary: Improving the production of complex viral glycoproteins in plants can be achieved by engineering glycosylation-directed folding and combining chaperone coexpression with glyco-engineering techniques.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Article
Immunology
Ananda S. Bandyopadhyay, Chris Gast, Elizabeth B. Brickley, Ricardo Ruttimann, Ralf Clemens, M. Steven Oberste, William C. Weldon, Margaret E. Ackerman, Ruth Connor, Wendy F. Wieland-Alter, Peter Wright, Vytautas Usonis
Summary: The study demonstrated that IPV-immunized children showed improved type 2 poliovirus protection after receiving mOPV2, with a significant reduction in viral shedding after the second dose. Before the challenge, intestinal immunity against type 2 poliovirus was undetectable in these children, but some developed intestinal neutralizing antibodies after receiving the vaccine.
JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Immunology
Gwenn Waerlop, Yorick Janssens, Bart Jacobs, Franziska Jarczowski, Andre Diessner, Geert Leroux-Roels, Victor Klimyuk, Isabel Leroux-Roels, Frank Thieme
Summary: The development of an effective norovirus vaccine is crucial for reducing the global burden of norovirus-associated diseases. A clinical trial on 60 healthy adults showed that the norovirus vaccine candidate rNV-2v induced significant humoral and cellular immune responses, and cross-reactive immune response against different norovirus strains was observed.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Cell Biology
Etsuro Nanishi, Francesco Borriello, Timothy R. O'Meara, Marisa E. McGrath, Yoshine Saito, Robert E. Haupt, Hyuk-Soo Seo, Simon D. van Haren, Cecilia B. Cavazzoni, Byron Brook, Soumik Barman, Jing Chen, Joann Diray-Arce, Simon Doss-Gollin, Maria De Leon, Alejandra Prevost-Reilly, Katherine Chew, Manisha Menon, Kijun Song, Andrew Z. Xu, Timothy M. Caradonna, Jared Feldman, Blake M. Hauser, Aaron G. Schmidt, Amy C. Sherman, Lindsey R. Baden, Robert K. Ernst, Carly Dillen, Stuart M. Weston, Robert M. Johnson, Holly L. Hammond, Romana Mayer, Allen Burke, Maria E. Bottazzi, Peter J. Hotez, Ulrich Strych, Aiquan Chang, Jingyou Yu, Peter T. Sage, Dan H. Barouch, Sirano Dhe-Paganon, Ivan Zanoni, Al Ozonoff, Matthew B. Frieman, Ofer Levy, David J. Dowling
Summary: The global deployment of vaccines for COVID-19 is urgently needed, especially in low- and middle-income countries. The study found that a specific adjuvant formulation significantly increased neutralizing antibody levels and protected aged mice from SARS-CoV-2. These findings support the further development of this adjuvanted vaccine as an effective and affordable option.
SCIENCE TRANSLATIONAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Wenjuan Wei, Nouredine Behloul, Weiqi Wang, Sarra Baha, Zhenzhen Liu, Ruihua Shi, Jihong Meng
Summary: The study focused on developing oral vaccine nanoparticles using truncated capsid protein p146 for hepatitis E (HE) and utilized chitosan nanoparticles as a mucosal delivery system. The produced CS/p146 NPs exhibited stable dispersion, near-spherical shape with optimal size, high encapsulation efficiency, and low cytotoxicity with sustained-release effects. Immunogenicity experiments in mice showed that CS/p146 NPs induced higher antigen-specific systemic and mucosal immune responses compared to purified p146, highlighting their potential as HE oral vaccine candidates.
MACROMOLECULAR BIOSCIENCE
(2021)
Article
Immunology
Nsofwa Sukwa, Cynthia Mubanga, Luiza M. Hatyoka, Obvious N. Chilyabanyama, Mwelwa Chibuye, Samson Mundia, Masiliso Munyinda, Ethel Kamuti, Muyunda Siyambango, Sharif Badiozzaman, Samuel Bosomprah, Nils Carlin, Joanna Kaim, Bjorn Sjostrand, Michelo Simuyandi, Roma Chilengi, Ann-Mari Svennerholm
Summary: The study evaluated the safety, tolerability, and immunogenicity of an inactivated ETEC vaccine, ETVAX (R). The vaccine demonstrated good safety and tolerability in adults and children, and the 1/4 dose induced a stronger immune response in children.
Review
Immunology
Jorge Alberto, Salazar-Gonzalez, Omar Gonzalez-Ortega, Sergio Rosales-Mendoza
EXPERT REVIEW OF VACCINES
(2015)
Review
Biochemistry & Molecular Biology
Jorge A. Salazar-Gonzalez, Bernardo Banuelos-Hernandez, Sergio Rosales-Mendoza
PLANT MOLECULAR BIOLOGY
(2015)
Article
Plant Sciences
Elizabeth Monreal-Escalante, Dania O. Govea-Alonso, Marisela Hernandez, Jacquelynne Cervantes, Jorge A. Salazar-Gonzalez, Andrea Romero-Maldonado, Gabriela Rosas, Teresa Garate, Gladis Fragoso, Edda Sciutto, Sergio Rosales-Mendoza
Review
Immunology
Jorge A. Salazar-Gonzalez, Carlos Angulo, Sergio Rosales-Mendoza
Review
Immunology
Dania O. Govea-Alonso, Josue Beltran-Lopez, Jorge A. Salazar-Gonzalez, Juan Vargas-Morales, Sergio Rosales-Mendoza
EXPERT REVIEW OF VACCINES
(2017)
Review
Immunology
Sergio Rosales-Mendoza, Jorge A. Salazar-Gonzalez, Eva L. Decker, Ralf Reski
EXPERT REVIEW OF VACCINES
(2016)
Article
Immunology
Sergio Rosales-Mendoza, Jorge A. Salazar-Gonzalez
EXPERT REVIEW OF VACCINES
(2017)
Article
Biochemistry & Molecular Biology
Dania O. Govea-Alonso, Marlene A. Tello-Olea, Josue Beltran-Lopez, Elizabeth Monreal-Escalante, Jorge A. Salazar-Gonzalez, Bernardo Banuelos-Hernandez, Sergio Rosales-Mendoza
MOLECULAR BIOTECHNOLOGY
(2017)
Article
Plant Sciences
Elizabeth Monreal-Escalante, Abel A. Ramos-Vega, Jorge A. Salazar-Gonzalez, Bernardo Banuelos-Hernandez, Carlos Angulo, Sergio Rosales-Mendoza
Article
Biochemistry & Molecular Biology
Jorge Alberto Salazar-Gonzalez, Sergio Rosales-Mendoza, Andrea Romero-Maldonado, Elizabeth Monreal-Escalante, Edith Elena Uresti-Rivera, Bernardo Banuelos-Hernandez
MOLECULAR BIOTECHNOLOGY
(2014)
Article
Biotechnology & Applied Microbiology
Sergio Rosales-Mendoza, Nestor Rubio-Infante, Elizabeth Monreal-Escalante, Dania O. Govea-Alonso, Ana Lilia Garcia-Hernandez, Jorge A. Salazar-Gonzalez, Omar Gonzalez-Ortega, L. M. Teresita Paz-Maldonado, Leticia Moreno-Fierros
PLANT CELL TISSUE AND ORGAN CULTURE
(2014)
Article
Biochemistry & Molecular Biology
Jorge Alberto Salazar-Gonzalez, Alail Antonio Ruiz-Cruz, Ismael Bustos-Jaimes, Leticia Moreno-Fierros
MOLECULAR BIOTECHNOLOGY
(2019)
Review
Biotechnology & Applied Microbiology
Diana Isabel Tamayo-Ramos, Jorge Alberto Salazar-Gonzalez, Stuart A. Casson, Rafael Urrea-Lopez
Summary: This review summarises recent studies on avocado genetic transformation, highlighting the achievements and challenges in avocado breeding. It also discusses the potential application of new genetic engineering techniques in improving avocado traits.
PLANT CELL TISSUE AND ORGAN CULTURE
(2022)
Article
Biochemistry & Molecular Biology
Nestor Rubio-Infante, Dania O. Govea-Alonso, Andrea Romero-Maldonado, Ana Lilia Garcia-Hernandez, Damaris Ilhuicatzi-Alvarado, Jorge A. Salazar-Gonzalez, Schuyler S. Korban, Sergio Rosales-Mendoza, Leticia Moreno-Fierros
MOLECULAR BIOTECHNOLOGY
(2015)