Article
Immunology
Cheng-Chun Tai, Cheng-Hsien Tsai, Yu-Han Huang, Chia-Lin Lee, Hsin-Pai Chen, Yu-Jiun Chan
Summary: Respiratory viruses, particularly NIRVs, were frequently detected in adults hospitalized with RTIs, showing distinctive seasonal variations. A substantial number of RVs were identified in lower RT specimens or from patients admitted to ICU, highlighting their important role in causing severe respiratory infections.
JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION
(2021)
Article
Immunology
Yu-Ting Chiu, Ni Tien, Hsiao-Chuan Lin, Hsiu-Mei Wei, Huan-Cheng Lai, Jiun-An Chen, Yan-Yi Low, Hsiu-Hsien Lin, Yu-Lung Hsu, Kao-Pin Hwang
Summary: This study analyzed the epidemiology and clinical presentations of upper and lower respiratory tract infections. The positive rate of tested respiratory samples among adults and children was 21.48% and 48.31%, respectively. Human rhinovirus/Enterovirus (HRV/EV) was commonly found in all age groups, with a higher prevalence in upper respiratory tract infections, while Mycoplasma pneumoniae was the most common pathogen in lower respiratory tract infections.
JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION
(2022)
Article
Infectious Diseases
Xianxia Zhuo, Jiankang Zhao, Lei Wang, Bin Sun, Lanhua Sun, Chunlei Wang, Binbin Li, Yanyan Fan, Yingmei Liu, Bin Cao
Summary: This study aimed to establish a multiplex quantitative polymerase chain reaction (MQ-PCR) assay for 12 bacterial pathogens found in lower respiratory tract infection (LRTI) and evaluate its performance in a cohort of 211 LRTI patients. The MQ-PCR assay demonstrated a low limit of detection, high efficiency, and good sensitivity and specificity in the clinical validation study. This study suggests that the MQ-PCR assay is a time-saving and effective method for quantitative molecular detection of bacteria.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Microbiology
Linqing Zhao, Xiaoyan Dong, Ling Cao, Yongmei Jiang, Hong Zhang, Ran Mo, Hanmin Liu
Summary: This study evaluated the performance of Acaruiter Respiratory Panel (fluorescent PCR) in detecting respiratory tract infection pathogens in children. The results showed that it has good consistency, high accuracy, and high positive and negative agreement percentages.
MICROBIOLOGY SPECTRUM
(2023)
Article
Infectious Diseases
Laetitia Velly, Marta Cancella de Abreu, David Boutolleau, Ilaria Cherubini, Enfel Houas, Alexandre Aurousseau, Pierre Hausfater
Summary: This study aimed to investigate whether coupling molecular syndromic respiratory panel (RP) testing with procalcitonin (PCT) measurement improves antibiotic stewardship in lower respiratory tract infection in the emergency department. The results showed that displaying PCT and real-time RP results to emergency physicians did not significantly reduce antibiotic exposure. However, even in the standard of care group, the duration and initiation rate of antibiotics were already low with routine PCT measurement.
CLINICAL MICROBIOLOGY AND INFECTION
(2023)
Article
Medical Laboratory Technology
Liu Dong, Weijia Li, Qianqian Xu, Jianfei Gu, Zhihua Kang, Jian Chen, Xiao Xu, Xinju Zhang, Xiuming Zhang, Haoqin Jiang, Ming Guan
Summary: The study aimed to establish a new multiplex dPCR detection system that can distinguish exact species of malaria by one PCR reaction. The results showed that the dPCR system could rapidly diagnose and quantify malaria DNA samples, with low analytical sensitivity and good sensitivity and specificity in clinical samples.
CLINICA CHIMICA ACTA
(2023)
Article
Biochemical Research Methods
Enqi Huang, Yu Wang, Na Yang, Bowen Shu, Guohao Zhang, Dayu Liu
Summary: The Onestart system, utilizing a microfluidic chip, enables rapid and accurate detection of respiratory tract infection pathogens, with the entire analysis process completed within 1.5 hours. The system shows excellent specificity and stability in detecting pathogens.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Microbiology
Maelys Duclos, Benjamin Hommel, Florence Allantaz, Michaela Powell, Brunella Posteraro, Maurizio Sanguinetti
Summary: As SARS-CoV-2 continues to dominate the diagnostic strategies for respiratory tract infections (RTIs) during the current COVID-19 pandemic, this study provides evidence that various RTI pathogens may be circulating in the 13 countries included in the study. The findings suggest that the COVID-19 pandemic could potentially become endemic. Detecting respiratory pathogens other than SARS-CoV-2 in patients admitted to the emergency department for RTI symptoms could be useful for timely and appropriate clinical decision-making.
MICROBIOLOGY SPECTRUM
(2022)
Article
Infectious Diseases
Deyong Tan, Jianfeng Han, Qingzhi Sun, Xing Cheng, Juan Liu, Jia Liu, Qing Li, Lizhong Dai
Summary: In this study, a novel multiplex fluorescence quantitative PCR method was used to detect six common lower respiratory tract pathogens. The results showed high sensitivity and specificity of the method, as well as reduced duration of pathogen culture identification. This method has potential applications in clinical practice.
INFECTION AND DRUG RESISTANCE
(2023)
Article
Infectious Diseases
Nabil Gastli, Julien Loubinoux, Matthieu Daragon, Jean-Philippe Lavigne, Pierre Saint-Sardos, Helene Pailhories, Carole Lemarie, Hanaa Benmansour, Camille D'Humieres, Lauranne Broutin, Olivier Dauwalder, Michael Levy, Gabriel Auger, Solen Kerneis, Vincent Cattoir
Summary: The study evaluated the performance of FA-PP in detecting bacterial pathogens and antibiotic resistance genes in respiratory specimens, showing that it performed well in detecting pathogens in most specimens. The positive and negative percentage agreement values of FA-PP compared to culture were high, but there were some false-negative results. In addition, there was some discordance between the semi-quantification results of FA-PP and culture results.
CLINICAL MICROBIOLOGY AND INFECTION
(2021)
Article
Medicine, General & Internal
Christopher C. Butler, F. D. Richard Hobbs, Oghenekome A. Gbinigie, Najib M. Rahman, Gail Hayward, Duncan B. Richards, Jienchi Dorward, David M. Lowe, Joseph F. Standing, Judith Breuer, Saye Khoo, Stavros Petrou, Kerenza Hood, Jonathan S. Nguyen-Van-Tam, Mahendra G. Patel, Benjamin R. Saville, Joe Marion, Emma Ogburn, Julie Allen, Heather Rutter, Nick Francis, Nicholas P. B. Thomas, Philip Evans, Melissa Dobson, Tracie-Ann Madden, Jane Holmes, Victoria Harris, May Ee Png, Mark Lown, Oliver van Hecke, Michelle A. Detry, Christina T. Saunders, Mark Fitzgerald, Nicholas S. Berry, Lazaro Mwandigha, Ushma Galal, Sam Mort, Bhautesh D. Jani, Nigel D. Hart, Haroon Ahmed, Daniel Butler, Micheal McKenna, Jem Chalk, Layla Lavallee, Elizabeth Hadley, Lucy Cureton, Magdalena Benysek, Monique Andersson, Maria Coates, Sarah Barrett, Clare Bateman, Jennifer C. Davies, Ivy Raymundo-Wood, Andrew Ustianowski, Andrew Carson-Stevens, Ly-Mee Yu, Paul Little
Summary: This study aimed to determine the safety, effectiveness, and cost-effectiveness of molnupiravir in vaccinated high-risk patients in the community. The results showed that molnupiravir did not reduce hospital admissions or deaths associated with COVID-19 compared to usual care in this population.
Review
Medical Laboratory Technology
Jing Yang, Dandan Li, Jie Wang, Rui Zhang, Jinming Li
Summary: Viral respiratory infections are common and serious, and early diagnosis is crucial for effective management. Real-time reverse transcriptase PCR (rRT-PCR) is the best method for early diagnosis, and multiplex rRT-PCR can address various challenges associated with viral respiratory infections. However, the design and validation of multiplex rRT-PCR are more complex, and comprehensive research is lacking. This review provides guidance for designing and optimizing multiplex rRT-PCR detection panels and assesses the clinical utility of multiplex rRT-PCR in viral respiratory tract infection diagnosis.
CRITICAL REVIEWS IN CLINICAL LABORATORY SCIENCES
(2022)
Article
Respiratory System
Virve Enne, Alp Aydin, Rossella Baldan, Dewi R. Owen, Hollian Richardson, Federico Ricciardi, Charlotte Russell, Brenda O. Nomamiukor-Ikeji, Ann-Marie Swart, Juliet High, Antony Colles, Julie Barber, Vanya Gant, David M. Livermore, Justin O'Grady
Summary: Using PCR to directly search for pathogens and their resistance genes from clinical samples can improve treatment and stewardship. The study found that PCR testing had higher sensitivity than routine microbiology, detecting potential pathogens in patient samples reported as culture negative.
Article
Microbiology
Pierre Gazeau, Sophie Vallet, Severine Ansart, Clemence Beauruelle, Adissa Tran-Minoui, Christopher Payan, Lea Pilorge
Summary: Semi-quantitative PCR assay results can facilitate clinical interpretation of viral respiratory rapid multiplex PCR assays and critical evaluation of the results.
BRAZILIAN JOURNAL OF MICROBIOLOGY
(2021)
Review
Pathology
Lluis Moragues-Solanas, Riccardo Scotti, Justin O'Grady
Summary: Clinical metagenomics (CMg) offers a potential alternative to slow and insensitive microbiological culture for the diagnosis of nosocomial infections, particularly in distinguishing between BSI and LRTI. CMg approaches are more accurate in diagnosing LRTI due to higher pathogen numbers, while challenges remain for accurately diagnosing BSI. Advances in CMg for LRTI are likely to be implemented in hospitals within the next 2-5 years.
EXPERT REVIEW OF MOLECULAR DIAGNOSTICS
(2021)