Design, optimization, and application of multiplex rRT-PCR in the detection of respiratory viruses
Published 2022 View Full Article
- Home
- Publications
- Publication Search
- Publication Details
Title
Design, optimization, and application of multiplex rRT-PCR in the detection of respiratory viruses
Authors
Keywords
-
Journal
CRITICAL REVIEWS IN CLINICAL LABORATORY SCIENCES
Volume -, Issue -, Pages 1-18
Publisher
Informa UK Limited
Online
2022-05-14
DOI
10.1080/10408363.2022.2072467
References
Ask authors/readers for more resources
Related references
Note: Only part of the references are listed.- rRT-PCR for SARS-CoV-2: analytical considerations
- (2021) Rezgar Rahbari et al. CLINICA CHIMICA ACTA
- Fur: Find unique genomic regions for diagnostic PCR
- (2021) Bernhard Haubold et al. BIOINFORMATICS
- Xpert Xpress Flu/RSV: Validation and impact evaluation at a large UK hospital trust
- (2021) Thomas C Morris et al. JOURNAL OF MEDICAL VIROLOGY
- Convergent evolution of SARS-CoV-2 in human and animals
- (2021) Hang-Yu Zhou et al. Protein & Cell
- Rapid multiplex PCR assays in patients with respiratory viral infections: is semi-quantitative data useful? A pilot study
- (2021) Pierre Gazeau et al. BRAZILIAN JOURNAL OF MICROBIOLOGY
- Viral and bacterial coinfection among hospitalized children with respiratory tract infections
- (2020) Junxiao Liu et al. AMERICAN JOURNAL OF INFECTION CONTROL
- PCR inhibition in qPCR, dPCR and MPS—mechanisms and solutions
- (2020) Maja Sidstedt et al. ANALYTICAL AND BIOANALYTICAL CHEMISTRY
- Potential preanalytical and analytical vulnerabilities in the laboratory diagnosis of coronavirus disease 2019 (COVID-19)
- (2020) Giuseppe Lippi et al. CLINICAL CHEMISTRY AND LABORATORY MEDICINE
- Comparative Performance of SARS-CoV-2 Detection Assays Using Seven Different Primer-Probe Sets and One Assay Kit
- (2020) Arun K. Nalla et al. JOURNAL OF CLINICAL MICROBIOLOGY
- Current and Future Point-of-Care Tests for Emerging and New Respiratory Viruses and Future Perspectives
- (2020) Philipp P. Nelson et al. Frontiers in Cellular and Infection Microbiology
- Optimization of primer sets and detection protocols for SARS-CoV-2 of coronavirus disease 2019 (COVID-19) using PCR and real-time PCR
- (2020) Myungsun Park et al. EXPERIMENTAL AND MOLECULAR MEDICINE
- Use of Whole Genome Sequencing Data for a First in Silico Specificity Evaluation of the RT-qPCR Assays Used for SARS-CoV-2 Detection
- (2020) Mathieu Gand et al. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
- A recurrent mutation at position 26,340 of SARS-CoV-2 is associated with failure of the E-gene qRT-PCR utilized in a commercial dual-target diagnostic assay
- (2020) Maria Artesi et al. JOURNAL OF CLINICAL MICROBIOLOGY
- Impact of respiratory viral polymerase chain reaction testing on de‐escalation of antibiotic therapy in children who require chronic positive pressure ventilation
- (2020) Guillermo Beltran Ale et al. PEDIATRIC PULMONOLOGY
- Comparative analysis of primer-probe sets for RT-qPCR of COVID-19 causative virus (SARS-CoV-2)
- (2020) Yujin Jung et al. ACS Infectious Diseases
- Analytical sensitivity and efficiency comparisons of SARS-CoV-2 RT–qPCR primer–probe sets
- (2020) Chantal B. F. Vogels et al. Nature Microbiology
- Molecular diagnostic technologies for COVID-19: Limitations and challenges
- (2020) Adeel Afzal Journal of Advanced Research
- Discrimination of False Negative Results in RT-PCR Detection of SARS-CoV-2 RNAs in Clinical Specimens by Using an Internal Reference
- (2020) Yafei Zhang et al. VIROLOGICA SINICA
- Contaminations contaminate common databases
- (2020) Staffan Bensch et al. Molecular Ecology Resources
- Comparison of the Cepheid Xpert Xpress Flu/RSV assay and commercial real-time PCR for the detection of influenza A and influenza B in a prospective cohort from China
- (2019) Xiaohui Zou et al. INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
- The Ultimate qPCR Experiment: Producing Publication Quality, Reproducible Data the First Time
- (2019) Sean C. Taylor et al. TRENDS IN BIOTECHNOLOGY
- MFEprimer-3.0: quality control for PCR primers
- (2019) Kun Wang et al. NUCLEIC ACIDS RESEARCH
- Multiplex Platforms for the Identification of Respiratory Pathogens: Are They Useful in Pediatric Clinical Practice?
- (2019) Susanna Esposito et al. Frontiers in Cellular and Infection Microbiology
- Impact of multiplex respiratory virus testing on antimicrobial consumption in adults in acute care: a randomized clinical trial
- (2019) Elina Saarela et al. CLINICAL MICROBIOLOGY AND INFECTION
- Combining Procalcitonin and Rapid Multiplex Respiratory Virus Testing for Antibiotic Stewardship in Older Adult Patients With Severe Acute Respiratory Infection
- (2019) Chien-Chang Lee et al. Journal of the American Medical Directors Association
- Molecular point-of-care testing for influenza A/B and respiratory syncytial virus: comparison of workflow parameters for the ID Now and cobas Liat systems
- (2019) Stephen Young et al. JOURNAL OF CLINICAL PATHOLOGY
- Panther Fusion® Respiratory Virus Assays for the detection of influenza and other respiratory viruses
- (2019) Kathleen A. Stellrecht et al. JOURNAL OF CLINICAL VIROLOGY
- Performance of a multiplex PCR pneumonia panel for the identification of respiratory pathogens and the main determinants of resistance from the lower respiratory tract specimens of adult patients in intensive care units
- (2019) Sze Hwei Lee et al. JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION
- Comparison of the Panther Fusion and Allplex assays for the detection of respiratory viruses in clinical samples
- (2019) Lola Folgueira et al. PLoS One
- The Effect of Single Mismatches on Primer Extension
- (2018) Nick A. Rejali et al. CLINICAL CHEMISTRY
- Rapid and simple molecular tests for the detection of respiratory syncytial virus: a review
- (2018) Catherine A. Hogan et al. EXPERT REVIEW OF MOLECULAR DIAGNOSTICS
- Improved PCR flexibility with Hot Start dNTPs
- (2018) Tony Le et al. BIOTECHNIQUES
- Application of whole genome data for in silico evaluation of primers and probes routinely employed for the detection of viral species by RT-qPCR using dengue virus as a case study
- (2018) Kevin Vanneste et al. BMC BIOINFORMATICS
- Effects of secondary structures of DNA templates on the quantification of qPCR
- (2018) Huijun Fan et al. JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
- Can we distinguish respiratory viral infections based on clinical features? A prospective pediatric cohort compared to systematic literature review
- (2018) Xiaolin Ma et al. REVIEWS IN MEDICAL VIROLOGY
- Mutation and Epistasis in Influenza Virus Evolution
- (2018) Daniel Lyons et al. Viruses-Basel
- Rapid Detection of Respiratory Pathogens for Community-Acquired Pneumonia by Capillary Electrophoresis-Based Multiplex PCR
- (2018) Xue Li et al. SLAS Technology
- Preamplification with dUTP and Cod UNG Enables Elimination of Contaminating Amplicons
- (2018) Daniel Andersson et al. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
- Implementation of the cobas Liat influenza point-of-care test into an emergency department during a high-incidence season: a retrospective evaluation following real-world implementation
- (2018) J. Youngs et al. JOURNAL OF HOSPITAL INFECTION
- Viral Load Dynamics and Clinical Disease Severity in Infants With Respiratory Syncytial Virus Infection
- (2018) Cristina Garcia-Mauriño et al. JOURNAL OF INFECTIOUS DISEASES
- Comparing the Cobas Liat Influenza A/B and respiratory syncytial virus assay with multiplex nucleic acid testing
- (2018) Rainer Gosert et al. JOURNAL OF MEDICAL VIROLOGY
- More Pathogenicity or Just More Pathogens?—On the Interpretation Problem of Multiple Pathogen Detections with Diagnostic Multiplex Assays
- (2017) Andreas E. Zautner et al. Frontiers in Microbiology
- Multiplex PCR system for the rapid diagnosis of respiratory virus infection: systematic review and meta-analysis
- (2017) H.-S. Huang et al. CLINICAL MICROBIOLOGY AND INFECTION
- Rhinovirus Detection in Symptomatic and Asymptomatic Children: Value of Host Transcriptome Analysis
- (2016) Santtu Heinonen et al. AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
- The role of the NxTAG® respiratory pathogen panel assay and other multiplex platforms in clinical practice
- (2016) Susanna Esposito et al. EXPERT REVIEW OF MOLECULAR DIAGNOSTICS
- Coinfections of the Respiratory Tract: Viral Competition for Resources
- (2016) Lubna Pinky et al. PLoS One
- Molecular diagnosis of respiratory viruses
- (2015) Lucy K. Somerville et al. PATHOLOGY
- Variability of the Reverse Transcription Step: Practical Implications
- (2014) S. Bustin et al. CLINICAL CHEMISTRY
- Respiratory Tract Samples, Viral Load, and Genome Fraction Yield in Patients With Middle East Respiratory Syndrome
- (2014) Ziad A. Memish et al. JOURNAL OF INFECTIOUS DISEASES
- The Influence of Nucleotide Sequence and Temperature on the Activity of Thermostable DNA Polymerases
- (2014) Jesse L. Montgomery et al. JOURNAL OF MOLECULAR DIAGNOSTICS
- Guidelines for validation of qualitative real-time PCR methods
- (2014) S. Broeders et al. TRENDS IN FOOD SCIENCE & TECHNOLOGY
- A multiple-alignment based primer design algorithm for genetically highly variable DNA targets
- (2013) Johanna Brodin et al. BMC BIOINFORMATICS
- Influence of PCR Reagents on DNA Polymerase Extension Rates Measured on Real-Time PCR Instruments
- (2013) J. L. Montgomery et al. CLINICAL CHEMISTRY
- Single-Nucleotide Polymorphisms and Other Mismatches Reduce Performance of Quantitative PCR Assays
- (2013) S. Lefever et al. CLINICAL CHEMISTRY
- No impact of early real-time PCR screening for respiratory viruses on length of stay and use of antibiotics in elderly patients hospitalized with symptoms of a respiratory tract infection in a single center in Norway
- (2013) S. S. Hernes et al. EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
- Development and Validation of Duplex, Triplex, and Pentaplex Real-Time PCR Screening Assays for the Detection of Genetically Modified Organisms in Food and Feed
- (2013) Ingrid Huber et al. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
- Respiratory virus multiplex RT-PCR assay sensitivities and influence factors in hospitalized children with lower respiratory tract infections
- (2013) Jikui Deng et al. VIROLOGICA SINICA
- Primer-BLAST: A tool to design target-specific primers for polymerase chain reaction
- (2012) Jian Ye et al. BMC BIOINFORMATICS
- Seasonality of viral respiratory infections in Southeast of Brazil: the influence of temperature and air humidity
- (2012) Luiz Gustavo Gardinassi et al. BRAZILIAN JOURNAL OF MICROBIOLOGY
- Beyond Diagnostic Accuracy: The Clinical Utility of Diagnostic Tests
- (2012) P. M. M. Bossuyt et al. CLINICAL CHEMISTRY
- PCR inhibitors - occurrence, properties and removal
- (2012) C. Schrader et al. JOURNAL OF APPLIED MICROBIOLOGY
- Advances in multiplex PCR: balancing primer efficiencies and improving detection success
- (2012) Daniela Sint et al. Methods in Ecology and Evolution
- Multi-Path Quenchers: Efficient Quenching of Common Fluorophores
- (2011) Pete Crisalli et al. BIOCONJUGATE CHEMISTRY
- Viral Infections of the Lower Respiratory Tract: Old Viruses, New Viruses, and the Role of Diagnosis
- (2011) A. T. Pavia CLINICAL INFECTIOUS DISEASES
- Respiratory Syncytial Virus Load, Viral Dynamics, and Disease Severity in Previously Healthy Naturally Infected Children
- (2011) Chadi M. El Saleeby et al. JOURNAL OF INFECTIOUS DISEASES
- Molecular Diagnosis of Viral Respiratory Infections
- (2011) Shu Zhang et al. Current Infectious Disease Reports
- MPprimer: a program for reliable multiplex PCR primer design
- (2010) Zhiyong Shen et al. BMC BIOINFORMATICS
- Monovalent and divalent salt correction algorithms for Tm prediction--recommendations for Primer3 usage
- (2010) N. von Ahsen et al. BRIEFINGS IN BIOINFORMATICS
- Validation of Laboratory-Developed Molecular Assays for Infectious Diseases
- (2010) E. M. Burd CLINICAL MICROBIOLOGY REVIEWS
- 3′-Protected 2′-Deoxynucleoside 5′-Triphosphates as a Tool for Heat-Triggered Activation of Polymerase Chain Reaction
- (2009) Inna Koukhareva et al. ANALYTICAL CHEMISTRY
- Enhancing multiplex PCR efficiency using Hot Start dNTPs
- (2009) Tony Le et al. BIOTECHNIQUES
- The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments
- (2009) S. A. Bustin et al. CLINICAL CHEMISTRY
- Performance of laboratory diagnostics for the detection of influenza A(H1N1)v virus as correlated with the time after symptom onset and viral load
- (2009) Peter K.C. Cheng et al. JOURNAL OF CLINICAL VIROLOGY
- Evaluation of tetramethylrhodamine and black hole quencher 1 labeled probes and five commercial amplification mixes in TaqMan® real-time RT-PCR assays for respiratory pathogens
- (2009) Genyan (Patrick) Yang et al. JOURNAL OF VIROLOGICAL METHODS
- Incubation periods of acute respiratory viral infections: a systematic review
- (2009) Justin Lessler et al. LANCET INFECTIOUS DISEASES
- Detection of Respiratory Viruses by Molecular Methods
- (2008) J. B. Mahony CLINICAL MICROBIOLOGY REVIEWS
- Optimal Sampling Sites and Methods for Detection of Pathogens Possibly Causing Community-Acquired Lower Respiratory Tract Infections
- (2008) K. Loens et al. JOURNAL OF CLINICAL MICROBIOLOGY
Add your recorded webinar
Do you already have a recorded webinar? Grow your audience and get more views by easily listing your recording on Peeref.
Upload NowBecome a Peeref-certified reviewer
The Peeref Institute provides free reviewer training that teaches the core competencies of the academic peer review process.
Get Started