Article
Cell & Tissue Engineering
Helena Isla-Magrane, Anna Veiga, Jose Garcia-Arumi, Anna Duarri
Summary: A two-step method was established to obtain self-organized multizone ocular progenitor cells (mzOPCs) from 2D hiPSC cultures within three weeks, which were then grown in suspension to create 3D multiocular organoids. These organoids exhibited correct layering of retinal regions, resembled transparent ocular-surface epithelium in the corneal regions, and contained interactions between different cell types and regions of the eye system. This approach demonstrated the advantages of the combined 2D-3D construction tissue model in creating a more ocular native-like cellular environment for modeling eye development and disease.
STEM CELL RESEARCH & THERAPY
(2021)
Review
Cell & Tissue Engineering
Samaneh Dehghan, Reza Mirshahi, Alireza Shoae-Hassani, Masood Naseripour
Summary: This study discusses age-related macular degeneration (AMD) and the current treatment options for wet and dry AMD. Stem cell therapy has shown great potential in the treatment of AMD. Human pluripotent stem cells, especially induced pluripotent stem cells (iPSCs), can be used to generate retinal pigment epithelium (RPE) cells, restoring the structure and function of the retina.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Biotechnology & Applied Microbiology
Mohamed A. Faynus, Jeffrey K. Bailey, Britney O. Pennington, Mika Katsura, Duncan A. Proctor, Ashley K. Yeh, Sneha Menon, Dylan G. Choi, Jane S. Lebkowski, Lincoln Johnson, Dennis O. Clegg
Summary: Dry age-related macular degeneration (AMD) is a common eye disease with no current treatment options. Using microcarrier technology for cell culture allows efficient large-scale production of therapeutic retinal pigmented epithelial cells, fulfilling the treatment demands.
BIOENGINEERING-BASEL
(2022)
Article
Ophthalmology
Marina Yasvoina, Qian Yang, Sasha M. Woods, Tjebo Heeren, Grant M. Comer, Catherine A. Egan, Marcus Fruttiger
Summary: This study found that RPE cells invading the retina in retinal degenerative diseases undergo dedifferentiation and can be pigmented or unpigmented. As a result, the number of these cells may be underestimated through funduscopy.
BRITISH JOURNAL OF OPHTHALMOLOGY
(2022)
Article
Neurosciences
Una Greferath, Mario Huynh, Andrew Ian Jobling, Kirstan Anne Vessey, Gene Venables, Denver Surrao, Helen Christine O'Neill, Ioannis J. Limnios, Erica Lucy Fletcher
Summary: Retinitis pigmentosa is a group of inherited retinal degenerations characterized by gradual loss of photoreceptors, ultimately leading to irreversible vision loss. This study focused on analyzing the changes in glial, microglial, and photoreceptor cells in different retinal locations in RCS rats, revealing significant differences in photoreceptor loss between the dorsal and ventral retina. The findings suggest that breakdown of the outer limiting membrane may play a role in exacerbating photoreceptor loss in the ventral retina, highlighting the value of using the RCS rat model for sectorial retinitis pigmentosa.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2021)
Article
Medicine, Research & Experimental
Qian Liu, Jun Liu, Minmei Guo, Tzu-Cheng Sung, Ting Wang, Tao Yu, Zeyu Tian, Guoping Fan, Wencan Wu, Akon Higuchi
Summary: This study investigated the effectiveness of subretinal transplantation of various types of stem cells in treating retinal degeneration (RD). The results showed that all types of stem cells, except hiPSCs, improved visual function up to 4 weeks post-injection. However, only rats that received hiPSC-derived RPE cells maintained their visual function at 8 weeks post-injection.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Xiaowen Mao, Seta Stanbouly, Jacob Holley, Michael Pecaut, James Crapo
Summary: The aim of this study was to investigate the acute oxidative damage in ocular structure and retinal function after exposure to spaceflight, and evaluate the effectiveness of an antioxidant in mitigating spaceflight-induced changes in the retina. Results showed that spaceflight conditions induced retinal oxidative stress, leading to photoreceptor cell damage and impaired retinal function.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biology
Sandy Gozlan, Vivien Batoumeni, Tara Fournier, Celine Nanteau, Anais Potey, Marilou Clemencon, Gael Orieux, Jose-Alain Sahel, Olivier Goureau, Jerome E. Roger, Sacha Reichman
Summary: Researchers have developed a simple method to generate, expand, and cryopreserve retinal progenitor cells derived from induced pluripotent stem cells. These cells have the potential to be used in cell-based therapies and drug discovery for retinal diseases.
COMMUNICATIONS BIOLOGY
(2023)
Article
Cell Biology
Hongtao Shen, Jing Li, Tyler Heisler-Taylor, Ryan Makin, Huan Yang, Timur A. Mavlyutov, Bradley Gelfand, Colleen M. Cebulla, Lian-Wang Guo
Summary: The absence of TMEM97 in RPE cells disrupts redox-balancing systems, leading to increased oxidative stress and loss of photoreceptors. TMEM97 KO also affects proteins crucial to mitochondrial and lysosomal stability, as well as impeding autophagy flux.
CELLULAR SIGNALLING
(2021)
Article
Biochemistry & Molecular Biology
Kohei Homma, Eriko Toda, Hideto Osada, Norihiro Nagai, Takumi Era, Kazuo Tsubota, Hideyuki Okano, Yoko Ozawa
Summary: Mitochondrial dysfunction in MELAS patient-derived induced pluripotent stem cells resulted in impaired proliferation and survival when glycolysis was inhibited, which was rescued by taurine treatment. Metabolomic analysis revealed oxidative stress and EMT in the retinal pigment epithelium differentiated from MELAS iPSCs, both of which were alleviated by taurine. Therefore, taurine may serve as a potential therapeutic approach for mitochondrial-related retinal diseases.
Article
Biochemistry & Molecular Biology
Yuqin Liang, Feng Tan, Xihao Sun, Zekai Cui, Jianing Gu, Shengru Mao, Hon Fai Chan, Shibo Tang, Jiansu Chen
Summary: This study established an iPSCs model of a PRPF6-related adRP patient and generated a disease model of RPE cells, revealing the effects of PRPF6 mutation on the morphology, gene expression, and function of RPE cells. This research is of great significance for understanding PRPF6-related RPE cells and future cell therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Sudipta Mahato, Trupti Agrawal, Divya Pidishetty, Savitri Maddileti, Vinay Kumar Pulimamidi, Indumathi Mariappan
Summary: This protocol describes a simplified, robust, and stepwise method of generating retinal organoids using a hybrid culture system. The resulting organoids contain proliferating, multipotent retinal precursors that self-assemble to form mature retinal organoids.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Article
Cell Biology
Shu-Chun Kuo, Chung-Ching Chio, Chao-Hung Yeh, Jui-Ti Ma, Wen-Pin Liu, Mao-Tsun Lin, Kao-Chang Lin, Ching-Ping Chang
Summary: In vivo and in vitro studies showed that A beta(1-42) oligomer affects retinal morphology and cell viability, leading to dysmorphological changes and decreased expression of tight junction proteins. Conditioned medium from mesenchymal stem cells protected against A beta-induced retinal pathology by influencing various signaling pathways and protein expressions. Secretomes from MSC-CM might have potential therapeutic implications in age-related macular degeneration.
Article
Biochemistry & Molecular Biology
Maria Hytti, Eveliina Korhonen, Heidi Hongisto, Kai Kaarniranta, Heli Skottman, Anu Kauppinen
Summary: The study showed that iPSC-RPE cells from AMD patients exhibit increased secretion of IL-1β and reduced levels of IL-6 and MCP-1 when protein clearance was partially inhibited. This suggests that dysfunctional cellular clearance mechanisms may already be present in cells originating from AMD patients, leading to inflammasome activation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Medicine, General & Internal
Hyunchae Sim, Wonhwa Lee, Samyeol Choo, Eui Kyun Park, Moon-Chang Baek, In-Kyu Lee, Dong Ho Park, Jong-Sup Bae
Summary: A study evaluated the protective effects of SFN from cruciferous vegetables against PM2.5-induced oxidative damage, showing that SFN effectively alleviates oxidative damage in human ARPE-19 cells via its antioxidant effects.
FRONTIERS IN MEDICINE
(2021)
Article
Ophthalmology
Maria Cinta Puell, Ines Contreras, Isabel Pinilla, Jose Juan Escobar, Antonio Soler-Garcia, Antonio Javier Blasco, Pablo Lazaro
Summary: By synthesizing scientific evidence and expert panel judgment, a range of patient-reported outcomes and clinical outcome measures recommended for patients with age-related macular degeneration and diabetic retinopathy were identified. Ophthalmologists can choose the most suitable outcome measure for their patients based on the recommended measures, shifting clinical practice towards patient-centered medicine.
EUROPEAN JOURNAL OF OPHTHALMOLOGY
(2021)
Article
Medicine, General & Internal
Elvira Orduna-Hospital, Judit Otero-Rodriguez, Lorena Perdices, Ana Sanchez-Cano, Ana Boned-Murillo, Javier Acha, Isabel Pinilla
Summary: The study showed that for type 1 diabetes patients without diabetic retinopathy, there is a decline in retinal sensitivity with age and disease progression. Additionally, there is a direct correlation between retinal sensitivity and macular thickness in the DM1 group.
Article
Ophthalmology
A. Boned-Murillo, M. D. Diaz-Barreda, A. Ferreras, I Bartolome-Sese, E. Orduna-Hospital, P. Montes-Rodriguez, J. Ascaso, Isabel Pinilla
Summary: The study used OCT and microperimetry to evaluate structural and functional ocular changes in patients with type 2 diabetes and moderate diabetic retinopathy without diabetic macular edema. The results showed that macular GCC thickness and central retinal sensitivity were reduced in the patients with moderate DR, indicating the presence of macular neurodegeneration.
GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY
(2021)
Letter
Ophthalmology
Ana Boned-Murillo, Maria D. Diaz-Barreda, Isabel Pinilla
GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY
(2022)
Article
Medicine, General & Internal
Guisela Fernandez-Espinosa, Ana Boned-Murillo, Elvira Orduna-Hospital, Maria Dolores Diaz-Barreda, Ana Sanchez-Cano, Sofia Bielsa-Alonso, Javier Acha, Isabel Pinilla
Summary: This study evaluated retinal vascularization in patients with type 2 diabetes mellitus and moderate diabetic retinopathy using optical tomography angiography. The results showed a decrease in vascular density and an increase in foveal avascular zone area, with significant correlations to disease duration and age.
Review
Biochemistry & Molecular Biology
Ana Boned-Murillo, Henar Albertos-Arranz, Maria Dolores Diaz-Barreda, Elvira Orduna-Hospital, Ana Sanchez-Cano, Antonio Ferreras, Nicolas Cuenca, Isabel Pinilla
Summary: Optical coherence tomography angiography (OCTA) is an essential tool for the diagnosis and control of diabetic patients, allowing visualization and analysis of the retinal and choroidal microvasculature, and the detection of diabetic retinopathy and other related diseases.
Review
Biochemistry & Molecular Biology
Isabel Pinilla, Victoria Maneu, Laura Campello, Laura Fernandez-Sanchez, Natalia Martinez-Gil, Oksana Kutsyr, Xavier Sanchez-Saez, Carla Sanchez-Castillo, Pedro Lax, Nicolas Cuenca
Summary: Inherited retinal dystrophies (IRDs) are a group of genetically and clinically heterogeneous diseases characterized by progressive degeneration of the retina and loss of visual function. Oxidative stress and inflammation play key roles in the pathophysiology of IRDs. Gene therapy has shown some success in treating IRDs, but other therapeutic strategies targeting oxidative stress and inflammation are also being investigated.
Editorial Material
Biochemistry & Molecular Biology
Isabel Pinilla, Victoria Maneu
Article
Biochemistry & Molecular Biology
Guisela Fernandez-Espinosa, Elvira Orduna-Hospital, Ana Boned-Murillo, Maria Dolores Diaz-Barreda, Ana Sanchez-Cano, Maria Sopena-Pinilla, Isabel Pinilla
Summary: This study aimed to investigate the choroidal thickness in patients with moderate diabetic retinopathy and its correlation with retinal thickness compared to healthy subjects. The results showed that retinal thickness was higher in certain areas in the diabetic group, but choroidal thickness did not differ between the groups. Additionally, age was found to have an impact on retinal thickness and choroidal thickness.
Article
Health Care Sciences & Services
Elvira Orduna-Hospital, Maria Arcas-Carbonell, Ana Sanchez-Cano, Isabel Pinilla, Alejandra Consejo
Summary: The study compared the retinal and choroidal layers in DM1 patients without DR signs with healthy subjects using speckle contrast of OCT images as a tissue biomarker. Results showed significant differences in thickness and speckle contrast of the inner retinal layer in DM1 patients, indicating potential retinal neurodegeneration before the onset of DR.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Meeting Abstract
Ophthalmology
Isabel Pinilla, Isabel Bartolome, Ma Dolores Diaz-Barreda, Ana Boned-Murillo, Guisela Fernandez-Espinosa, Maria Sopena-Pinilla, Elvira Orduna Hospital
ACTA OPHTHALMOLOGICA
(2022)
Article
Medicine, General & Internal
Mikel Garcia Gomez de Segura, Ana Martin-Arroyuelos, Isabel Pinilla, Javier Araiz
Summary: The study assessed differences in macular thickness evolution after uncomplicated cataract surgery between non-diabetic subjects and diabetic patients without diabetic retinopathy using SD-OCT. Results showed that there was no increase in foveal, parafoveal, and perifoveal retinal thickness on postoperative day 7, but thickness values increased on days 30, 90, and 180 after surgery in both groups, peaking at 90 days. There were no differences in macular thickness before or after surgery between diabetic and non-diabetic patients.
Article
Health Care Sciences & Services
Guisela Fernandez-Espinosa, Carlos Ruiz-Tabuenca, Elvira Orduna-Hospital, Isabel Pinilla, Francisco J. Salgado-Remacha
Summary: The aim of this study was to reduce the variability of manual segmentation of the Foveal Avascular Zone (FAZ) in retinal optical coherence tomography angiography (OCTA) images. A new criterion was established based on the comparison of segmentations by different observers, resulting in smaller FAZ areas and slightly reduced acircularity values in all groups.
JOURNAL OF PERSONALIZED MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Ana Boned-Murillo, Guisela Fernandez-Espinosa, Elvira Orduna-Hospital, Maria Dolores Diaz-Barreda, Ana Sanchez-Cano, Maria Sopena-Pinilla, Sofia Bielsa-Alonso, Isabel Pinilla
Summary: This study analyzes the retinal thickness and sensitivity in DM2 patients and correlates the results with blood lipid levels and MAIA measurements, providing an accurate method for assessing moderate DR in DM2 patients. Thinning of GCL+ and GCL++ may support ganglion cell loss before RNFL damage.
Article
Construction & Building Technology
Silvia Ezpeleta, Elvira Orduna-Hospital, Teresa Solana, Justiniano Aporta, Isabel Pinilla, Ana Sanchez-Cano
Summary: The study analyzed melanopic light in educational environments and proposed an improvement plan based on LED lighting to optimize classroom lighting and ensure adequate light for users at any time of day. The results simplify lighting projects and enable designers to carry out optimized evaluations of specific environments from both the photometric and circadian perspectives.
Article
Ophthalmology
He-Yan Li, Li Dong, Xu-Han Shi, Rui-Heng Zhang, Wen-Da Zhou, Hao-Tian Wu, Chu-Yao Yu, Yi-Tong Li, Yi-Fan Li, Jost B. Jonas, Wen-Bin Wei, Yue-Ming Liu
Summary: This study aimed to examine the intraocular tolerability of cetuximab when applied intravitreally, and its effect on axial elongation. The results showed that intravitreal injections of cetuximab could reduce axial elongation in a dose-dependent and number of treatment-dependent manner, without causing intraocular toxic effects.
EXPERIMENTAL EYE RESEARCH
(2024)
Article
Ophthalmology
James D. Cole, John A. McDaniel, Joelle Nilak, Ashley Ban, Carlos Rodriguez, Zuhaad Hameed, Marta Grannonico, Peter A. Netland, Hu Yang, Ignacio Provencio, Xiaorong Liu
Summary: Aniridia is a panocular condition characterized by the loss of iris, caused mainly by mutations in the PAX6 gene. This study assessed the effects of Pax6-haploinsufficiency on retinal morphology and vision in Pax6Sey mice. The results showed elevated intraocular pressure and declining visual acuity in Pax6Sey mice, along with local retinal damage and a possible neuroinflammatory response.
EXPERIMENTAL EYE RESEARCH
(2024)
Article
Ophthalmology
Subao Jiang, Ning Xia, Francesco Buonfiglio, Elsa W. Boehm, Qi Tang, Norbert Pfeiffer, Dominik Olinger, Huige Li, Adrian Gericke
Summary: This study found that a high-fat diet induces endothelial dysfunction in the ophthalmic artery, leading to impaired vascular function in the eye. The high-fat diet triggers oxidative stress and involves RAGE and NOX2.
EXPERIMENTAL EYE RESEARCH
(2024)
Review
Ophthalmology
Mala Upadhyay, Vera L. Bonilha
Summary: The sodium iodate (NaIO3) model is a valuable tool in studying dry AMD and evaluating the retinal structure. Through extensive research, it has been found that the death of RPE cells caused by NaIO3 injection is closely related to vision loss in patients with dry AMD. Therefore, studying NaIO3 can provide insights into the mechanisms of RPE and photoreceptor cell death and contribute to understanding RPE degeneration in AMD.
EXPERIMENTAL EYE RESEARCH
(2024)
Article
Ophthalmology
Vicki Chrysostomou, Katharina C. Bell, Sze Woei Ng, Samyuktha Suresh, Gayathri Karthik, Marion Millet, Yingying Chung, Jonathan G. Crowston
Summary: We present a new experimental model for inducing retinal ganglion cell (RGC) dysfunction and degeneration in mice. This model allows for the reliable induction of RGC dysfunction and loss, with the resilience of the optic nerve being dependent on the age of the animal. The time-defined nature of the functional and structural changes observed in this model will facilitate the study of glial responses and the evaluation of neuroprotective treatments after intraocular pressure (IOP) injury.
EXPERIMENTAL EYE RESEARCH
(2024)
