Article
Biochemistry & Molecular Biology
Sharad V. Jaswandkar, Kalpana S. Katti, Dinesh R. Katti
Summary: This study investigates the impact of ADF/cofilin on actin filaments using molecular dynamics simulations. The results show that the binding of ADF/cofilin modifies the conformation and alignment of actin filaments locally, leading to filament severing at the boundaries. The findings provide important insights into the cofilin-mediated actin dynamics.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2022)
Article
Microbiology
Melissa C. Steele-Ogus, Richard S. Johnson, Michael J. MacCoss, Alexander R. Paredez
Summary: The study identified and localized 46 interactors of polymerized actin in Giardia cells, highlighting the importance of GlActin in multiple cellular processes. Surprisingly, eight of these proteins were found to localize to the ventral disc, an organelle crucial for host attachment in Giardia, suggesting a potential target for new drugs to combat drug resistance in Giardiasis.
MICROBIOLOGY SPECTRUM
(2021)
Article
Biochemistry & Molecular Biology
Tatsuya Niwa, Yuhei Chadani, Hideki Taguchi
Summary: The Lon protease has been identified as the major protease involved in the degradation of obligate GroE substrates in GroE-depleted cells. Deletion of other major E. coli chaperones does not significantly affect the folding of these substrates, indicating that their folding is primarily dependent on GroE.
Article
Biochemistry & Molecular Biology
Jared Sivinski, Duc Ngo, Christopher J. Zerio, Andrew J. Ambrose, Edmond R. Watson, Lynn K. Kaneko, Marius M. Kostelic, Mckayla Stevens, Anne-Marie Ray, Yangshin Park, Chunxiang Wu, Michael T. Marty, Quyen Q. Hoang, Donna D. Zhang, Gabriel C. Lander, Steven M. Johnson, Eli Chapman
Summary: This study outlines the structural similarities and functional differences between ESKAPE GroES/GroEL and Escherichia coli GroES/GroEL, and reveals the importance of allosteric compatibility between these two proteins for cell viability. Interestingly, differences in allosteric compatibility do not necessarily result in differences in refolding rates.
Article
Multidisciplinary Sciences
Changping Li, Baoxu Ding, Xintong Ma, Xuan Yang, Hongyan Wang, Yuefan Dong, Zhibin Zhang, Jinbin Wang, Xiaochong Li, Yanan Yu, Yiyang Yu, Bao Liu, Jonathan F. Wendel, Yidan Li, Tianya Wang, Lei Gong
Summary: In this study, we investigated the cytonuclear coordination during RuBisCo biogenesis in multiple plant allopolyploids and found similar genomic and transcriptional responses. Our observations reveal the potential widespread features of cytonuclear evolution and provide insight into the folding and assembly process of RuBisCo.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Biochemistry & Molecular Biology
Hideki Taguchi, Ayumi Koike-Takeshita
Summary: Protein folding can be hindered by protein aggregation, but this can be prevented by chaperones in the cell. The bacterial chaperonin GroEL forms complexes with its cochaperonin GroES to facilitate protein folding. Recent studies have identified numerous GroE-dependent clients, shedding light on the role of chaperonins in protein folding and evolution.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Article
Multidisciplinary Sciences
Liufeng Dai, Baojie Wang, Ting Wang, Etienne H. Meyer, Valentin Kettel, Natalie Hoffmann, Heather E. McFarlane, Shalan Li, Xuna Wu, Kelsey L. Picard, Patrick Giavalisco, Staffan Persson, Yi Zhang
Summary: Energy is crucial for cellular functions, but how actin dynamics are regulated by ATP levels in plant cells is not well understood. This study reveals an important role for TORC1 in coordinating ATP homeostasis and actin dynamics in plant cells.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Biochemistry & Molecular Biology
Jing Gao, Fumihiko Nakamura
Summary: This review article introduces actin-associated proteins (AAPs) and their roles in regulating cell movement, shape change, division, organelle localization, and trafficking. The article lists all discovered AAPs and allows sorting based on various criteria. It also provides links to databases for accessing detailed information about protein structures, expression levels, mutations, and pathology. Additionally, small molecules targeting actin and AAPs with potential for treating diseases are listed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Cell Biology
Heba Ghozlan, Amanda Cox, Daniel Nierenberg, Stephen King, Annette R. Khaled
Summary: Maintenance of the cellular proteome is crucial for cell function, and its deregulation can lead to diseases. Chaperonins, such as CCT, play a central role in folding proteins and preventing aggregation. CCT has unique features that allow it to fold large or complex proteins and complexes. Understanding the structure, function, and interactome of CCT is essential for studying its role in disease and potential diagnostic and therapeutic applications.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Pharmacology & Pharmacy
Coralie Mergault, Fanny Lisee, Victor Tiroille, Melia Magnien, Christelle Parent, Woodys Lenga Mabonda, Damien Sizaret, Madeleine Jaillet, Bruno Crestani, Sylvain Marchand-Adam, Laurent Plantier
Summary: The study found that the expression of the Arp2/3 complex was increased in profibrotic environments in vitro and in vivo. Inhibition of the Arp2/3 complex repressed ACTA2 and COL1 expression and blocked the Akt/phospho-GSK3 beta/beta-catenin/MRTF-A pathway in lung fibroblasts. CK666 demonstrated antifibrotic properties in the lung in vivo.
BRITISH JOURNAL OF PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Siddhi Chitre, Anne-Marie Ray, Mckayla Stevens, Emma H. Doud, Hope Liechty, Alex Washburn, Katelyn Tepper, Jared Sivinski, Heather M. O'Hagan, Millie M. Georgiadis, Eli Chapman, Steven M. Johnson
Summary: Protein homeostasis in tumor progression has been proven to be important, but little is known about the therapeutic potential of targeting the HSP60 chaperonin system. This study suggests that targeting aberrant HSP60 in the cytosol could be a promising chemotherapeutic strategy for cancer treatment.
BIOORGANIC & MEDICINAL CHEMISTRY
(2022)
Article
Microbiology
Jared Sivinski, Andrew J. Ambrose, Iliya Panfilenko, Christopher J. Zerio, Jason M. Machulis, Niloufar Mollasalehi, Lynn K. Kaneko, Mckayla Stevens, Anne-Marie Ray, Yangshin Park, Chunxiang Wu, Quyen Q. Hoang, Steven M. Johnson, Eli Chapman
Summary: The GroES/GroEL chaperonin system in bacteria is essential and potentially a target for antibiotics. However, the functional differences between GroES/GroEL chaperonins from different bacteria, including ESKAPE pathogens, suggest potential challenges in developing chaperonin-targeted antibiotics.
Review
Biochemistry & Molecular Biology
Vikash Kumar, Ankita Behl, Rumaisha Shoaib, Mohammad Abid, Maxim Shevtsov, Shailja Singh
Summary: In this review, we summarize the information on prefoldin family members in archaea and humans, and analyze the unexplored subunits of Plasmodium falciparum (Pf). By comparing with the homologs in archaea and H. sapiens, we enhance the understanding of the functions, structure, and substrate binding mechanism of Pf prefoldin. Our review also highlights the importance of certain PfPFD subunits in malaria parasite biology, as well as their up-regulation during different stages of the parasite life cycle.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Article
Biochemistry & Molecular Biology
Micaela Boiero Sanders, Christopher P. Toret, Audrey Guillotin, Adrien Antkowiak, Thomas Vannier, Robert C. Robinson, Alphee Michelot
Summary: The use of different actin isoforms in eukaryotic cells and the molecular mechanisms of their segregation into distinct networks are poorly understood. By using yeast as a model, researchers found that the expression of heterologous actin causes significant reorganization of the actin cytoskeleton. However, the expression of two heterologous actin variants, each specialized in assembling a different network, can rescue cytoskeletal organization and increase resistance to external perturbation.
Article
Multidisciplinary Sciences
Joseph Puccini, Jia Wei, Liang Tong, Dafna Bar-Sagi
Summary: Macropinocytosis is a nonselective endocytosis process that facilitates the uptake of extracellular fluid-phase cargoes. Tumor cells exploit this process to acquire macromolecules for cell growth and proliferation under nutrient-limiting conditions. This study reveals that the enzyme ATP citrate lyase (ACLY) is a key regulator of macropinocytosis, and it is involved in the actin cytoskeleton. The findings demonstrate a mechanism that links regional acetyl-CoA availability with dynamic protein acetylation, supporting the spatial and temporal modulation of membrane-cytoskeletal interactions required for macropinocytosis.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
