Article
Developmental Biology
Fumiyasu Imai, Mike Adam, S. Steven Potter, Yutaka Yoshida
Summary: The study reveals the crucial role of HoxD family transcription factors in the specificity of monosynaptic sensory-motor connections in the spinal cord. Mice lacking Hoxd9, Hoxd10 and Hoxd11 show locomotion defects and aberrant axon development in quadriceps motor neurons.
Article
Engineering, Biomedical
Stefan Harmsen, Emin Ilker Medine, Maxim Moroz, Fuad Nurili, Jose Lobo, Yiyu Dong, Mezruh Turkekul, Naga Vara Kishore Pillarsetty, Richard Ting, Vladimir Ponomarev, Oguz Akin, Omer Aras
Summary: The adoptive cell transfer of targeted chimeric antigen receptor (CAR) T cells is a promising cancer therapy, and a dual-modal PET/NIRF nanoparticle-based imaging agent has been developed to monitor their biodistribution and fate in vivo, providing a new approach for imaging technology in other cancer models.
Article
Neurosciences
Susumu Tanaka, Soju Seki, Yudai Ono, Akifumi Enomoto, Mikihiko Kogo
Summary: The study revealed that orexin-A (Ox-A) can affect the membrane properties of mesencephalic trigeminal (Mes V) neurons in several ways, such as shortening the after-hyperpolarization duration, increasing interspike frequency during repetitive discharge, enhancing resonance, and increasing sodium current density. These effects indicate that Ox-A may enhance Mes V neuronal excitability by modulating various membrane properties.
NEUROSCIENCE LETTERS
(2021)
Article
Biochemistry & Molecular Biology
Uma Anand, Praveen Anand, Mikael Hans Sodergren
Summary: Terpenes in Cannabis sativa inhibit capsaicin-induced calcium influx by activating Na+/K+ ATPase, which contributes to their analgesic effects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biology
Katiuscia Martinello, Antonietta Sucapane, Sergio Fucile
Summary: Rat dorsal root ganglion neurons expressing 5-HT3 receptors play an important role in sensory signaling modulation and neurotransmitter release. The 5-HT3 receptors in adult DRG neurons are permeable to Ca2+ ions and can increase neurotransmitter release.
Article
Neurosciences
Patricia Perez-Garcia, Ricardo Pardillo-Diaz, Noelia Geribaldi-Doldan, Ricardo Gomez-Oliva, Samuel Dominguez-Garcia, Carmen Castro, Pedro Nunez-Abades, Livia Carrascal
Summary: Understanding the development of specialized neural circuits in adult mammals requires studying the changes in neuronal membrane properties and circuits during postnatal maturation. Research on layer V pyramidal neurons in the rat primary motor cortex showed that certain parameters are established at birth, while other properties continue to develop until adulthood. These modifications lead to a decrease in neuronal excitability and an increase in working range, providing more sensitive and accurate responses in young adult neurons.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2021)
Article
Neurosciences
Li-Kang Zhang, Jia-Hui Sun, Sheng Liu, Wen-Liang Zhang, Jian-Long Zou
Summary: In this study, we developed an in vitro model called SCWR to mimic the tissue-specific differences between motor and sensory nerve regeneration. The model demonstrated asymmetric regeneration of the ventral and dorsal roots in response to growth factors and exhibited motor and sensory Schwann cell phenotypes. Additionally, we identified a targeted effect of collagen VI on sensory nerve bundle formation and characterized the protein expression profiles associated with motor/sensory-specific nerve regeneration.
EXPERIMENTAL NEUROLOGY
(2023)
Article
Nanoscience & Nanotechnology
Shan Jiang, Tianyan Wang, Sandra Behren, Ulrika Westerlind, Kornelia Gawlitza, Jenny L. Persson, Knut Rurack
Summary: This study presents a strategy for ratiometric STn detection and dual-color cancer cell labeling using molecularly imprinted polymers (MIPs). The synthesized dual fluorescent STn-MIPs allowed for dual-color labeling of cancer cells, providing improved accuracy compared to single-signal MIPs. The concept of the luminescent core-fluorescent MIP shell architecture can be applied to other target analytes.
ACS APPLIED NANO MATERIALS
(2022)
Article
Medicine, General & Internal
Chang-Hoon Koo, Jiseok Baik, Hyun-Jung Shin, Jin-Hee Kim, Jung-Hee Ryu, Sung-Hee Han
Summary: The study found that lidocaine and bupivacaine have neurotoxic effects on developing spinal cord motor neurons, while ropivacaine has less impact on them.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Eveljn Scarian, Matteo Bordoni, Valentina Fantini, Emanuela Jacchetti, Manuela Teresa Raimondi, Luca Diamanti, Stephana Carelli, Cristina Cereda, Orietta Pansarasa
Summary: This study demonstrates the robustness of 3D cultures for the differentiation of stem cells in studying ALS, using bioprinting and iPSCs. The results show that the hydrogel-based constructs do not interfere with the differentiation process and electrophysiological features of the cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Daniel E. Russ, Ryan B. Patterson Cross, Li Li, Stephanie C. Koch, Kaya J. E. Matson, Archana Yadav, Mor R. Alkaslasi, Dylan Lee, Claire E. Le Pichon, Vilas Menon, Ariel J. Levine
Summary: Single-cell RNA sequencing data is used to generate a comprehensive cell atlas of mouse post-natal spinal cord, revealing the hierarchical relationships among cell types and providing spatial analysis of their distribution. Additionally, an open-source cell type classifier, SeqSeek, is developed to facilitate standardized cell type identification.
NATURE COMMUNICATIONS
(2021)
Article
Neurosciences
Andoni Asencor, Gennady Dvoryanchikov, Vivien Makhoul, Pantelis Tsoulfas, Nirupa Chaudhari
Summary: Due to their ease of use, AAVs are important tools in neuroscience. However, they have been underutilized in the study of peripheral sensory neurons due to the lack of selective targeting methods. The introduction of the AAV-PHP.S capsid with enhanced tropism for peripheral neurons has provided a solution. This study demonstrates the successful targeting of mouse sensory ganglia using AAV-PHP.S, allowing for visualization of terminals and selective expression in specific cell types.
Article
Neurosciences
Diego N. Messina, Emanuel D. Peralta, Cristian G. Acosta
Summary: The expression of the K2P channel TREK2 in neurons is regulated by GDNF, and this finding may have therapeutic potential in the treatment of chronic pain.
EXPERIMENTAL NEUROLOGY
(2022)
Article
Multidisciplinary Sciences
Katarina Milicevic, Andrej Korenic, Milena Milosevic, Pavle R. Andjus
Summary: This protocol outlines the preparation and characterization of primary cultures of glial cells, astrocytes, and microglia from rat cortices for studying ALS pathophysiology. It includes isolation and culture of astrocytes and microglia, immunocytochemical testing for purity, and dye-loading and Ca2+ recording techniques for live cell imaging experiments. Video recordings demonstrate distinct responses of ALS IgG in hSOD1(G93A) astrocytes compared to non-transgenic controls.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Article
Cell Biology
Jing-Jing Cui, Jia Wang, Dong-Sheng Xu, Shuang Wu, Ya-Ting Guo, Yu-Xin Su, Yi-Han Liu, Yu-Qing Wang, Xiang-Hong Jing, Wan-Zhu Bai
Summary: Neural tract tracing using AF488-CTB was effective in labeling motor, sensory, and sympathetic neurons with clear and complete morphology, but image quality deteriorated after 14 days post-injection.
NEURAL REGENERATION RESEARCH
(2022)