Article
Food Science & Technology
Hodan Ibrahim, Jacquie Maignel, Fraser Hornby, Donna Daly, Matthew Beard
Summary: This review discusses the current evidence of BoNT/A's effect on bladder sensation, potential mechanisms by which it exerts these effects, and recent advances in understanding its action in bladder tissue.
Article
Multidisciplinary Sciences
Zheng Liu, Pyung-Gang Lee, Nadja Krez, Kwok-ho Lam, Hao Liu, Adina Przykopanski, Peng Chen, Guorui Yao, Sicai Zhang, Jacqueline M. Tremblay, Kay Perry, Charles B. Shoemaker, Andreas Rummel, Min Dong, Rongsheng Jin
Summary: The binding and uptake of botulinum neurotoxin E (BoNT/E) requires the glycosylation of its host receptor SV2A with high selectivity for SV2A and SV2B over SV2C. The interaction between BoNT/E and SV2 is mediated through both protein-protein and protein-glycan associations, which are essential for BoNT/E to enter cells and exert neurotoxicity.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Yuan-Hong Jiang, Jia-Fong Jhang, Yu-Khun Lee, Hann-Chorng Kuo
Summary: Low-energy shock wave (LESW) therapy, especially when combined with intravesical therapeutic agents, has shown promising results in treating chronic prostatitis and bladder disorders. This novel treatment model has the potential to significantly reduce pain and inflammation, improve symptoms, and enhance the quality of life for patients with IC/BPS.
Article
Food Science & Technology
Juliana Y. Bushnell, Lindsay N. Cates, Jeffrey E. Hyde, Christoph P. Hofstetter, Claire C. Yang, Zin Z. Khaing
Summary: Early bladder chemodenervation after severe spinal cord injury (SCI) can reduce bladder fibrosis and hypertrophy, thus protecting bladder tissue integrity.
Article
Biology
Laurent Cotter, Feifan Yu, Sylvain Roqueviere, Juliette Duchesne de Lamotte, Johannes Krupp, Min Dong, Camille Nicoleau
Summary: A cell-based assay for detecting SNAP25-cleaving Botulinum neurotoxins (BoNTs) is developed by combining hiPSC-derived motor neurons and a luminescent detection system based on split NanoLuc luciferase. This assay is convenient, rapid, free-of-specialized antibodies, with a detection sensitivity of femtomolar concentrations of toxin, and can be used to study the different steps of BoNT intoxication.
COMMUNICATIONS BIOLOGY
(2023)
Article
Chemistry, Medicinal
Wei-Yi Wu, Shih-Pin Lee, Bing-Juin Chiang, Wei-Yu Lin, Chiang-Ting Chien
Summary: The study confirmed that urothelial CaSR can regulate micturition function, with experiments demonstrating the effects of CaSR agonists and antagonists on bladder smooth muscle activity. Intravesical administration of AC inhibited micturition reflex, bladder nerve activities, and reversed bladder hyperactivity, indicating the chemosensory function of urothelial CaSR. Therefore, the study provided further evidence that urothelial CaSR could modulate micturition reflex and be a potential pharmacotherapeutic target for bladder disorders.
Review
Food Science & Technology
Shu-Yu Wu, Shang-Jen Chang, Stephen Shei-Dei Yang, Chun-Kai Hsu
Summary: The study evaluated the use of BTX-A injection in children with medically refractory neurogenic bladder, finding it to be a safe and effective treatment option. Improvements in clinical and urodynamic parameters were observed as the main outcomes of the treatment.
Article
Biochemistry & Molecular Biology
Merja Joensuu, Parnayan Syed, Saber H. Saber, Vanessa Lanoue, Tristan P. Wallis, James Rae, Ailisa Blum, Rachel S. Gormal, Christopher Small, Shanley Sanders, Anmin Jiang, Stefan Mahrhold, Nadja Krez, Michael A. Cousin, Ruby Cooper-White, Justin J. Cooper-White, Brett M. Collins, Robert G. Parton, Giuseppe Balistreri, Andreas Rummel, Frederic A. Meunier
Summary: It has been found that botulinum neurotoxin type A (BoNT/A) targets nerve terminals by binding to two receptors on the neuronal plasma membrane: polysialoganglioside (PSG) and synaptic vesicle glycoprotein 2 (SV2). The entry of BoNT/A into synaptic vesicles (SVs) requires a tripartite surface nanocluster, which consists of a preassembled PSG-synaptotagmin-1 (Syt1) complex and SV2 on the neuronal plasma membrane. This tripartite nanocluster controls the endocytic sorting of the toxin into synaptic vesicles.
Article
Computer Science, Artificial Intelligence
Chuan Zhang, Yen-Ting Chen, Yang Liu, Elaine Magat, Monica Gutierrez-Verduzco, Gerard E. Francisco, Ping Zhou, Sheng Li, Yingchun Zhang
Summary: This study aimed to improve the efficiency of botulinum toxin (BoNT) in managing spasticity, utilizing a three-dimensional innervation zone imaging (3DIZI) technique based on high-density surface electromyography (HD-sEMG) recordings. Results showed a significant improvement in spasticity management at the 3-week follow-up, with the experimental group demonstrating higher treatment efficiency compared to the control group.
INTERNATIONAL JOURNAL OF NEURAL SYSTEMS
(2021)
Article
Pharmacology & Pharmacy
Qinggang Liu, Yi Gao, Huiling Cong, Limin Liao
Summary: This study aimed to investigate the safety and feasibility of ultrasound-assisted intravesical delivery of BTX-A and its potential benefits in a rat model of bladder hyperactivity induced by acetic acid instillation. The results showed that ultrasound-assisted intravesical delivery of BTX-A led to cleavage of SNAP-25, inhibition of calcitonin gene-related peptide release from afferent nerve terminals, and amelioration of acetic acid-induced bladder hyperactivity.
FRONTIERS IN PHARMACOLOGY
(2023)
Review
Food Science & Technology
Fan-Ching Hung, Hann-Chorng Kuo
Summary: Botulinum toxin A (BoNT-A) intravesical injections have been used to treat refractory functional bladder disorders. Liposomes, as drug delivery vehicles, can transport BoNT-A into cells. Liposomal BoNT-A can reduce frequency and urgency of urination, improve bladder pain and may have a promising role in treating bladder oversensitivity.
Article
Biochemistry & Molecular Biology
Friederike Schuemann, Oliver Schmitt, Andreas Wree, Alexander Hawlitschka
Summary: In Parkinson's disease, hypercholinism in the striatum leads to disturbed motor functions. Injecting Botulinum neurotoxin-A into the striatum of Parkinsonian rats resulted in increased immunoreactivity for cleaved SNAP-25 in brain regions other than the injected striatum, suggesting a bidirectional axonal transport of BoNT-A to connected parts of the brain. The distance from the injected striatum and the time until maximum immunoreactivity was reached showed a linear relationship.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Natalia Zeber-Lubecka, Maria Kulecka, Katarzyna Zaleska-Oracka, Michalina Dabrowska, Aneta Balabas, Ewa E. Hennig, Magdalena Szymanek-Szwed, Michal Mikula, Beata Jurkiewicz, Jerzy Ostrowski
Summary: The aim of this study is to determine the molecular differences between the bladder body and trigonal urothelia. The analysis of transcriptomes and microbiomes revealed functional differences between the two regions and significant differences in bacterial taxa in urine.
Article
Rehabilitation
Yu-Hui Huang, Sung-Lang Chen
Summary: This study investigated the effects of concomitant injections of botulinum toxin-A (BoNT-A) into the detrusor and external urethral sphincter muscles in suprasacral spinal cord injured patients with detrusor overactivity and detrusor sphincter dyssynergia. The results showed that this treatment decreased detrusor and urethral pressure without increasing post-void residual ratio and diaper pad use. It may provide an optional alternative for spinal cord injury patients who are unwilling or unable to increase catheterization frequency.
JOURNAL OF REHABILITATION MEDICINE
(2022)
Review
Food Science & Technology
Hsiang-Ying Lee, Hann-Chorng Kuo
Summary: This article reviewed the use of botulinum toxin type A (BTX-A) injections into the bladder wall for men with small prostates without bladder outlet obstruction (BOO). The results showed that BTX-A injections were effective and safe for treating overactive bladders (OAB) in men, particularly in those without prior prostate surgery. Patients with prior radical prostatectomy (RP) also had better efficacy and lower risk of side effects. However, the pathophysiology mechanism of OAB in men may differ from that in women, which could affect the efficacy of BTX-A injection.
Article
Urology & Nephrology
Afonso Morgado, Alberto Costa Silva, Paulo Diniz, Carlos Martins Silva
Summary: The prevalence of normal TT/low cFT is high among patients with ED, which is often overlooked by current screening recommendations for MH. Aging seems to be the main factor, as SHBG prevalence increases steeply with age. Current guidelines for MH screening should be amended.
INTERNATIONAL JOURNAL OF IMPOTENCE RESEARCH
(2023)
Review
Medicine, General & Internal
Pedro Abreu-Mendes, Aurora Costa, Ana Charrua, Rui Almeida Pinto, Francisco Cruz
Summary: There is a trend towards a significant association between increased VEGF levels and pain or symptom severity in BPS/IC patients. Although there are some discrepancies among the studies and the number of patients included is small, VEGF and its receptors should be considered for future studies regarding its use in BPS/IC pathophysiology, diagnosis, and prognostication.
Article
Urology & Nephrology
Luis Vale, Ana Charrua, Helena Cavaleiro, Rita Ribeiro-Oliveira, Antonio Avelino, Tiago Antunes-Lopes, Antonio Albino-Teixeira, Francisco Cruz
Summary: This study aimed to investigate the urinary ATP levels and the incidence of detrusor underactivity (DU) in the initial phases of bladder outlet obstruction (BOO), as well as their changes after deobstruction. The results showed that DU occurred more frequently after pBOO, and bladders with DU had significantly lower levels of ATP.
ADVANCES IN UROLOGY
(2022)
Review
Neurosciences
Catarina Reis, Silvia Chambel, Ana Ferreira, Celia Duarte Cruz
Summary: Pain is a complex experience and neuropathic pain poses a challenge for researchers and clinicians. Nerve growth factor (NGF) is considered a key player in neuropathic pain, but the toxic adverse effects of anti-NGF therapeutics limit its clinical use. Further research is needed to improve treatment for chronic neuropathic pain.
REVIEWS IN THE NEUROSCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Ana Ferreira, Silvia Sousa Chambel, Antonio Avelino, Celia Duarte Cruz
Summary: Traumatic spinal cord injury leads to changes in the structure of the urethral wall, affecting urethral function and resulting in urinary dysfunction.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Food Science & Technology
Pedro Abreu-Mendes, Antonio Ferrao-Mendes, Francisco Botelho, Francisco Cruz, Rui Pinto
Summary: This study aimed to evaluate the long-term efficacy and adverse events of intratrigonal botulinum toxin injection in patients with bladder pain syndrome/interstitial cystitis. The results showed that the intratrigonal injection of botulinum toxin was an effective and safe long-term treatment for patients refractory to conservative forms of treatment. Age, basal pain intensity, and time to injection request did not predict long-term response to OnaBotA.
Review
Economics
Francisco Cruz, Natalya Danchenko, Kyle Fahrbach, Andreas Freitag, Jialu Tarpey, John Whalen
Summary: This study compared the efficacy and safety of abobotulinumtoxinA (aboBoNT-A) and onabotulinumtoxinA (onaBoNT-A) for the treatment of refractory neurogenic detrusor overactivity (NDO) using an indirect treatment comparison. The results showed that abobotulinumtoxinA may be more effective than onabotulinumtoxinA in reducing the frequency of urinary incontinence episodes. Rating: 8 out of 10.
JOURNAL OF MEDICAL ECONOMICS
(2023)
Review
Biochemistry & Molecular Biology
Ana Ferreira, Diogo Nascimento, Celia Duarte Cruz
Summary: Neurogenic detrusor overactivity (NDO) is a severe lower urinary tract disorder caused by neurologic lesion, and animal models, particularly spinal cord injury (SCI) models, have been crucial for understanding the molecular mechanisms of NDO. Inflammatory processes, regulation of cell survival, and changes in neuronal receptors have been identified as key mechanisms in NDO. However, most studies have relied on SCI models, which may limit the translation of findings to other NDO-driven pathologies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Daniel H. H. Pozza, Isaura Tavares, Celia Duarte Cruz, Sara Fonseca
Summary: This systematic review aimed to identify high-risk patients and summarise the causes, consequences, and management/recommendations of spinal cord injuries (SCI) due to neuraxial techniques in anaesthesia. The main risk factors reported were extremes of age, obesity, and diabetes. Anaes-SCI was reported as a consequence of various factors and mainly resulted in motor deficits, sensory loss, and pain. Despite potential complications, neuraxial techniques are still a beneficial option for pain prevention and management, but careful patient management and close monitoring are crucial to minimize the risk of SCI and complications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Cell Biology
Silvia Sousa Chambel, Celia Duarte Cruz
Summary: Axonal growth inhibitors play an important role in traumatic injuries to the adult mammalian central nervous system, inhibiting axonal regeneration. These inhibitors are produced by neurons, oligodendrocytes, and astrocytes, and exert their effects by binding to specific receptors. Currently, there are no approved pharmacological treatments for spinal cord injuries, but ongoing clinical trials are investigating novel therapies targeting these inhibitory proteins and/or their receptors.
NEURAL REGENERATION RESEARCH
(2023)
Review
Biochemistry & Molecular Biology
Ana Catarina Neto, Mariana Santos-Pereira, Pedro Abreu-Mendes, Delminda Neves, Henrique Almeida, Francisco Cruz, Ana Charrua
Summary: The definitions of chronic pelvic/visceral pain have changed within international societies, greatly impacting research approaches. Recent studies highlight the significance of systemic changes and the central nervous system in the perpetuation of pelvic/visceral pain. As a result, researchers are utilizing animal models that replicate systemic changes, rather than organ- or tissue-specific models. This review discusses the advantages and disadvantages of bladder-centric and systemic models, highlighting central nervous system changes and pain-related behaviors. It also raises concerns about potential investigator bias and suggests new approaches for studying chronic pelvic/visceral pain through refined or novel animal models.
Review
Urology & Nephrology
Michel Wyndaele, Ana Charrua, Francois Herve, Patrik Aronsson, Luke Grundy, Vik Khullar, Alan Wein, Paul Abrams, Francisco Cruz, Celia Duarte Cruz
Summary: Inflammation and neuronal hypersensitivity are common reactions after urothelial injury, but how these reactive mechanisms develop into chronic disease in some patients is still unclear. Chronic inflammation plays a significant role in lower urinary tract dysfunctions, but the underlying mechanisms are not fully understood.
NEUROUROLOGY AND URODYNAMICS
(2023)
Meeting Abstract
Urology & Nephrology
S. Chambel, R. Oliveira, A. Ferreira, M. Schwab, Antunes T. Lopes, C. Cruz
Meeting Abstract
Urology & Nephrology
A. Ferreira, S. Chambel, A. Avelino, T. Antunes Lopes, C. Duarte Cruz