Article
Multidisciplinary Sciences
PinFen Chua, William K. Lim
Summary: This study investigated the applications of different PC12 variants in neuroprotection research, identifying optimal culture conditions for PC12 Riken and NS-1 cells, and determining collagen IV as the preferred adhesive substrate for both cell lines. In the in vitro stroke model, NS-1 cells achieved a maximal differentiation of 72.7% after optimization, and the 5-HT1A agonist 8-OH-DPAT was found to be neuroprotective in PC12 cells.
SCIENTIFIC REPORTS
(2021)
Article
Food Science & Technology
Fang Zhao, Xiaohan Zou, Shaoheng Li, Jing He, Chuchu Xi, Qinglian Tang, Yujing Wang, Zhengyu Cao
Summary: The research identified a new scorpion toxin, BmK NSPK, which enhances neurite extension through increasing the release of nerve growth factor and TrkA signaling pathway. This provides a new theoretical basis for developing treatments for spinal cord neuron development and regeneration using K-v channels.
Article
Cell Biology
Nessma Sultan, Laila E. Amin, Ahmed R. Zaher, Mohammed E. Grawish, Ben A. Scheven
Summary: Dental pulp stem cells secrete neurotrophic factors that significantly promote cell survival and neurite outgrowth in PC12 cells, with NGF, BDNF, and GDNF playing important roles. These factors may have potential therapeutic applications for peripheral nerve repair.
NEURAL REGENERATION RESEARCH
(2021)
Article
Engineering, Electrical & Electronic
Bjarke Norrehvedde Jensen, Yuting Wang, Alice Le Friec, Sadegh Nabavi, Mingdong Dong, Dror Seliktar, Menglin Chen
Summary: A wireless electromagnetic neural stimulation patch has been developed, which combines electromagnetic induction and physical guidance cues to promote neural cell growth and treat neurological diseases. In vitro experiments confirmed the biocompatibility and efficacy of the patch's electromagnetic stimulation in promoting neurite outgrowth, demonstrating its great potential for non-invasive neurological therapeutics.
NPJ FLEXIBLE ELECTRONICS
(2023)
Article
Multidisciplinary Sciences
Valdemar B. I. Johansen, Elizabeth Hampson, Elpida Tsonou, Chiara Pantarelli, Julia Y. Chu, Laraine Crossland, Hanneke Okkenhaug, Andrew J. Massey, David C. Hornigold, Heidi C. E. Welch, Stephen A. Chetwynd
Summary: Norbin is an adaptor protein that plays important roles in neuronal function and GPCR trafficking. This study provides new insights into the cellular functions of Norbin, showing that it regulates cell size, growth, and viability in PC12 cells. Additionally, Norbin controls the steady-state surface levels and agonist-induced internalisation of the GPCR S1PR1, suggesting it has a broader role in GPCR trafficking than previously thought. Furthermore, Norbin is involved in the activation of signaling pathways in PC12 cells. These findings enhance our understanding of the cellular functions of Norbin and its role in GPCR trafficking.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Gabrielle Edwards, Taaliah Campbell, Veronica Henderson, Alira Danaher, Daqing Wu, Rekha Srinivasan, Khosrow Rezvani, Valerie A. Odero-Marah
Summary: The study demonstrates that prostate cancer cells promote migration and adhesion to nerve cells through Snail expression, as well as neurite outgrowth via secretion of soluble factors. Targeting cancer cell interaction with nerves may be a potential strategy to halt prostate cancer progression/metastasis.
Article
Biochemistry & Molecular Biology
Marta R. Casanova, Rui L. Reis, Albino Martins, Nuno M. Neves
Summary: A biofunctional electrospun fibrous mesh (eFM) was developed by selectively retrieving nerve growth factor (NGF) from rat blood plasma, which can enhance neurite outgrowth and promote neuronal differentiation for peripheral nerve injury.
Article
Multidisciplinary Sciences
Anna-Kathrine Pedersen, Anamarija Pfeiffer, Gopal Karemore, Vyacheslav Akimov, Dorte B. Bekker-Jensen, Blagoy Blagoev, Chiara Francavilla, Jesper Olsen
Summary: This study investigated the roles of E3 ubiquitin ligases Cbl and Cbl-b in regulating signaling responses associated with neuroblastoma cell differentiation. By using mass spectrometry-based proteomics, the study identified SHP-2 and CDK16 as key regulators of extracellular signal-regulated kinase phosphorylation and neurite outgrowth in neuroblastoma, highlighting their involvement in the differentiation process.
Article
Cell Biology
SunYoung Park, Ji-Hwan Park, Un-Beom Kang, Seong-Kyoon Choi, Ahmed Elfadl, H. M. Arif Ullah, Myung-Jin Chung, Ji-Yoon Son, Hyun Ho Yun, Jae-Min Park, Jae-hyuk Yim, Seung-Jun Jung, Sang-Hyup Kim, Young-Chul Choi, Dae-Seong Kim, Jin-Hong Shin, Jin-Sung Park, Keun Hur, Sang-Han Lee, Eun-Joo Lee, Daehee Hwang, Kyu-Shik Jeong
Summary: Among the three isoforms encoded by Rtn4, Nogo-A has been extensively studied as a CNS inhibitor. Increased Nogo-A expression in various muscle-related pathological conditions suggests a role in muscle regeneration. Nogo(-/-) mice exhibit dystrophic muscle structure, impaired regeneration, and altered gene expression, indicating the importance of Nogo-A in muscle homeostasis and regeneration.
CELL DEATH DISCOVERY
(2021)
Article
Biochemistry & Molecular Biology
Juliane Meissner, Maryam Rezaei, Isabel Siepe, Doreen Ackermann, Simone Koenig, Johannes A. Eble
Summary: Proteomics studies have shown that adhesomes are made up of a variety of proteins at cellular contact sites with the extracellular matrix. The redox state of individual adhesome proteins in response to environmental signals adds a new aspect to adhesome complexity. This redox proteomic analysis revealed that sulfenylation and protein location at adhesomes mutually influence each other.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Biology
Junjie Jiang, Yuanchen Yu, Zhiwu Zhang, Yuan Ji, Hong Guo, Xiaohua Wang, Shengjun Yu
Summary: The expression of Nogo-A and its receptor increased after sciatic nerve injury in rats, suggesting an inhibitory role in the repair process. Immediate repair led to decreased protein expression and alleviation of pathological changes, showing better outcomes compared to delayed repair.
BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Takeru Koga, Hideyuki Ito, Yuji Iwaoka, Toshiro Noshita, Akihiro Tai
Summary: Isorhamnetin-3-O-glucoside and astragalin, found in the petals of Paeonia lactiflora, were identified as compounds that promote neurite outgrowth. They showed strong activity and could potentially be used as functional food ingredients to prevent cognitive decline.
Article
Behavioral Sciences
Yingchun Xiang, Yumiao Niu, Yacong Xie, Shishuo Chen, Feng Zhu, Weida Shen, Ling-Hui Zeng
Summary: The study investigated the phosphorylation levels of the RhoA/Rho kinase pathway in response to KA treatment, and found that KA induces activation of the pathway leading to inhibition of neurite outgrowth. Pretreatment with fasudil reversed this effect, suggesting that inhibiting the RhoA/Rho kinase pathway may be a potential treatment for seizure-induced neurite injury.
BRAIN AND BEHAVIOR
(2021)
Article
Chemistry, Medicinal
Ting Liang, Zhao Xie, Baiyun Dang, Jiayun Wang, Tongtong Zhang, Xiaofa Luan, Tao Lu, Chenyu Cao, Xin Chen
Summary: Novel indole-piperazine derivatives with a hydroxamic acid moiety were synthesized as selective HDAC6 inhibitors, showing nanomolar IC50 values in enzymatic assays. Among them, compound 9c exhibited the highest potency with an IC50 of 13.6 nM. In vitro studies showed that 9c induced neurite outgrowth and had neuroprotective effects against oxidative damage in PC12 cells, surpassing Tubastatin A. Western blot assay demonstrated that 9c increased the acetylation of alpha-tubulin in a dose-dependent manner.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2023)
Article
Biochemistry & Molecular Biology
Nana Bie, Xiaojuan Feng, Chenjing Li, Meng Meng, Chunling Wang
Summary: DHA protects PC12 cells from oxidative damage induced by H2O2 by increasing antioxidant capacity, mitochondrial function, and phosphorylation levels of signaling proteins; it also promotes neurite outgrowth and regulates neurotransmitter levels.
ACS CHEMICAL NEUROSCIENCE
(2021)
Article
Neurosciences
Kai Zhang, Yitan Yao, Kenji Hashimoto
Summary: Ketamine is a rapid antidepressant that can alleviate depressive symptoms within hours. However, its specific mechanisms of action are not yet clear, and it has several side effects. Understanding the pharmacological properties and mechanisms of ketamine can help develop a new generation of rapid antidepressants.
Article
Medicine, Research & Experimental
Ailin Luo, Zifeng Wu, Shan Li, Cindy B. McReynolds, Di Wang, Hanyu Liu, Chaoli Huang, Teng He, Xinying Zhang, Yuanyuan Wang, Cunming Liu, Bruce D. Hammock, Kenji Hashimoto, Chun Yang
Summary: This study investigated the relationship between chronic pain and depression and the potential benefits of inhibiting soluble epoxide hydrolase (sEH) signaling. The results showed that sEH expression was associated with pain and depression behaviors, and the sEH inhibitor TPPU improved the symptoms of both. The therapeutic effects of TPPU were mediated through the AHR and TSPO signaling pathways.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Article
Neurosciences
Zhiqian Yu, Kazuko Ueno, Ryo Funayama, Mai Sakai, Naoki Nariai, Kaname Kojima, Yoshie Kikuchi, Xue Li, Chiaki Ono, Junpei Kanatani, Jiro Ono, Kazuya Iwamoto, Kenji Hashimoto, Kengo Kinoshita, Keiko Nakayama, Masao Nagasaki, Hiroaki Tomita
Summary: This study investigated the transcriptional profiles in the dorsolateral prefrontal cortex (DLPFC) to understand the clinical and biological differences of schizophrenia between males and females. The results showed that females with schizophrenia had more significantly changed genes compared to males, and these changes were mainly related to mitochondrial, ATP- and metal ion-binding relevant biological processes. The differentially expressed genes related to schizophrenia in females were involved in midbrain dopaminergic and GABA-ergic neurons and microglia. Additionally, analysis of a murine model revealed that the methylation and transcriptional expression of the ACSBG1 locus potentially impact the sex differences in gene transcription.
MOLECULAR NEUROBIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Jie Guo, Di Qiu, Han-wen Gu, Xing-ming Wang, Kenji Hashimoto, Guang-fen Zhang, Jian-jun Yang
Summary: The perioperative administration of ketamine reduces postoperative depression and pain scores but increases the risk of adverse effects.
MOLECULAR PSYCHIATRY
(2023)
Article
Neurosciences
Xingming Wang, Akifumi Eguchi, Yong Yang, Lijia Chang, Xiayun Wan, Jiajing Shan, Youge Qu, Li Ma, Chisato Mori, Jianjun Yang, Kenji Hashimoto
Summary: Multiple sclerosis (MS) is a common demyelinating disease that affects the central nervous system. This study investigated the effects of subdiaphragmatic vagotomy (SDV) on demyelination in cuprizone (CPZ)-treated mice and found that SDV significantly improved demyelination and microglial activation in the brain. Analysis of gut microbiota composition and blood metabolites revealed that SDV also improved the abnormal levels in CPZ-treated mice. These findings suggest a link between the gut microbiota-brain axis and demyelination in the brain.
NEUROBIOLOGY OF DISEASE
(2023)
Correction
Neurosciences
X. Wang, A. Eguchi, Y. Yang, L. Chang, X. Wan, J. Shan, Y. Qu, L. Ma, C. Mori, J. Yang, K. Hashimoto
NEUROBIOLOGY OF DISEASE
(2023)
Article
Behavioral Sciences
Youge Qu, Lijia Chang, Li Ma, Xiayun Wan, Kenji Hashimoto
Summary: This study compared the effects of hallucinogenic psychedelic drug DOI, non-hallucinogenic psychedelic analog lisuride, and novel antidepressant (R)-ketamine on depression-like behavior and dendritic spine density in the brain. It was found that lisuride and (R)-ketamine improved these changes, while DOI did not. This suggests that the antidepressant-like effect of lisuride is not associated with 5-HT2AR-related psychedelic effects.
PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR
(2023)
Article
Clinical Neurology
Yong Yang, Akifumi Eguchi, Xiayun Wan, Lijia Chang, Xingming Wang, Youge Qu, Chisato Mori, Kenji Hashimoto
Summary: This study suggests that Chrna7 knockout mice exhibit depression-like behaviors and reduced expression of synaptic proteins in the medial prefrontal cortex through the gut-microbiota-brain axis via the subdiaphragmatic vagus nerve. Subdiaphragmatic vagotomy can block these depression-like behaviors and alter the composition of gut microbiota and microbiota-derived metabolites.
PROGRESS IN NEURO-PSYCHOPHARMACOLOGY & BIOLOGICAL PSYCHIATRY
(2023)
Article
Psychiatry
Youge Qu, Akifumi Eguchi, Xiayun Wan, Li Ma, Lijia Chang, Jiajing Shan, Yong Yang, Chisato Mori, Kenji Hashimoto
Summary: 3,4-Methylenedioxymethamphetamine (MDMA), the most widely used illicit compound worldwide, may contribute to stress resilience in mice subjected to chronic social defeat stress (CSDS) through the gut-microbiota-brain axis. The study found that MDMA treatment prevented the negative effects of CSDS, such as splenomegaly, anhedonia-like phenotype, and increased interleukin-6 (IL-6) levels in the mice. Gut microbiome analysis revealed differences between the saline + CSDS group and the MDMA + CSDS group, while metabolomics analysis showed altered plasma levels of N-epsilon-methyl-L-lysine in the saline + CSDS group compared to the control and MDMA + CSDS groups.
PSYCHIATRY RESEARCH
(2023)
Article
Clinical Neurology
Sai Chen, Jin-Jin Yang, Yue Zhang, Lei Lei, Di Qiu, Hui-Min Lv, Zhen-Tao Sun, Kenji Hashimoto, Jian-Jun Yang
Summary: This study investigated the effects of a single-dose of esketamine during anesthesia induction on emergence delirium (ED) in preschool children after minor surgery. The study found that the use of esketamine was associated with a higher incidence and score of ED, as well as a longer post-anesthesia care unit stay. However, there were no significant differences in other outcomes between the two groups. Therefore, the use of esketamine in preschool children for minor surgery should be noticed.
EUROPEAN ARCHIVES OF PSYCHIATRY AND CLINICAL NEUROSCIENCE
(2023)
Article
Neurosciences
Xiayun Wan, Akifumi Eguchi, Lijia Chang, Chisato Mori, Kenji Hashimoto
Summary: Patients with depression often have reduced bone mineral density. The new antidepressant arketamine has been shown to improve this reduction in mice. This study investigates the potential role of the gut microbiota and its metabolites in the beneficial effects of arketamine on behavioral and bone density changes in mice with chronic social defeat stress.
Article
Neurosciences
Yaoyu Pu, Qiuhong Wu, Qiuping Zhang, Tianwen Huang, Ji Wen, Long Wei, Kenji Hashimoto, Yi Liu
Summary: This study aimed to investigate the effect of mesenchymal stem-cell-derived microvesicles (MSC-MVs) in treating Parkinson's disease (PD). The results showed that single administration of MSC-MVs could alleviate the reduction of dopamine transporter and tyrosine hydroxylase expressions in the striatum and substantia nigra (SNr) of mice after MPTP injection. MSC-MVs also restored the abnormal gut microbiota composition induced by MPTP. Therefore, MSC-MVs have a new therapeutic potential for neurological disorders such as PD.
PSYCHOPHARMACOLOGY
(2023)
Article
Immunology
Xiayun Wan, Akifumi Eguchi, Akemi Sakamoto, Yuko Fujita, Yong Yang, Youge Qu, Masahiko Hatano, Chisato Mori, Kenji Hashimoto
Summary: Depletion of gut microbiota induced by antibiotic administration affects spleen and brain function, leading to decreased spleen weight and altered immune cell populations. Depletion of gut microbiota also leads to changes in metabolite levels, affecting spleen and brain function.
BRAIN, BEHAVIOR, & IMMUNITY - HEALTH
(2023)
Article
Psychiatry
Li Ma, Long Wang, Youge Qu, Xiayun Wan, Kenji Hashimoto
Summary: Relapse is common in remitted patients with major depressive disorder (MDD). Arketamine, an (R)-enantiomer of ketamine, has persistent prophylactic actions in an inflammatory model of depression. The heme biosynthesis II pathway in the spleen could be a new target for the prevention of relapse in MDD patients.
TRANSLATIONAL PSYCHIATRY
(2023)
Article
Neurosciences
Yukihiko Shirayama, Masaaki Iwata, Kanako Miyano, Yuki Hirose, Yasunori Oda, Yuko Fujita, Kenji Hashimoto
Summary: Beta-hydroxybutyrate (BHB) has shown antidepressant-like effects in a rat model of depression. It activates the BDNF-TrkB signaling pathway and AMPA receptors, and is associated with the prefrontal cortex, hippocampus, and basolateral region of amygdala.