Journal
EUROPEAN JOURNAL OF PHARMACOLOGY
Volume 649, Issue 1-3, Pages 29-37Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ejphar.2010.08.038
Keywords
mGlu2 receptor; Chinese hamster ovary cells; C6 glioma cell; Desensitization; cAMP accumulation; Protein kinase C; Spinal cord
Categories
Funding
- MRC
- Basque Government (Spain)
- MRC [G0601812] Funding Source: UKRI
- Medical Research Council [G0601812] Funding Source: researchfish
Ask authors/readers for more resources
To investigate the susceptibility of the group II metabotropic glutamate receptor mGlu2 to agonist-induced desensitization the receptor was stably expressed in Chinese hamster ovary (CHO mGlu2) or C6 glioma cells (C6 mGlu2) Exposure of CHO-mGlu2 cells to the group II mGlu receptor agonist (25 1'52'5) 2-(carboxycyclopropyl)glycine (LCCG 1 10 mu M) for up to 15 h did not affect the subsequent ability of LCCG-1 to inhibit forskolin stimulated cAMP accumulation Similarly in C6-mGlu2 cells prolonged exposure to LCCG 1 also did not affect the subsequent ability of LCCG-1 to inhibit cAMP formation In contrast exposure of CHO-mGlu2 cells to the protein kinase C activator phorbol myristate acetate (PMA) suppressed the ability of LCCG-1 to inhibit cAMP formation Using an in vitro model of group II mGlu receptor activity the hemisected neonatal rat spinal cord preparation the ability of the selective group II agonist (2R 4R)-4 aminopyrrolidine-2 4-dicarboxylate ((2R 4R)-APDC) to depress the fast component of the dorsal root-evoked ventral root potential (fDR VRP) did not desensitize when applied for up to 2 h Together these results indicate that in contrast to most G protein coupled receptors the mGlu2 receptor is resistant to agonist-induced homologous desensitization and that in vitro data suggests that resistance to desensitization is a physiologically relevant property of this mGlu receptor subtype (C) 2010 Elsevier B V All rights reserved
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available