Journal
EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY
Volume 116, Issue 10, Pages 1322-1331Publisher
WILEY
DOI: 10.1002/ejlt.201400020
Keywords
Biocatalysis; Cascade reactions; Lipase; Lipid modification; 3-MCPD; Metabolic engineering; Protein engineering; Structured triglycerides
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In this minireview, the application of enzymes for the modification of fats and oils is covered with special emphasis on lipase-catalyzed synthesis of structured triglycerides. Furthermore, protein engineering methods to alter the fatty acid chain-length or the trans-fatty acid selectivity of lipase A from Candida antarctica and an enzymatic two-step method to remove the contaminant 3-monochloro-1,2-propane diol are presented. Also the combination of several enzymes enables cascade reactions to obtain products such as v-hydroxycarboxylic acids useful for oleochemistry and recently published achievements to produce valuable compounds by metabolic engineering of microorganisms as exemplified for PUFA or fatty acid esters starting from sugars as renewable resources are covered. Special emphasis is given on achievements made in the authors group.
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