Journal
EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY
Volume 114, Issue 11, Pages 1254-1260Publisher
WILEY
DOI: 10.1002/ejlt.201100219
Keywords
Aqueous medium; L-a-glycerylphosphorylcholine; Phosphatidylcholine; Phospholipase A1 (Lecitase Ultra); Silica gel column chromatography
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Funding
- National High Technology Research and Development Program (863 Program) of China [2010AA101504]
- Key Projects in the National Science & Technology Pillar Program of China [2011BAD02B04]
- National Natural Science Foundation of China (NSFC) [31171703]
- Program for New Century Excellent Talents in University (NECT)
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L-alpha-Glycerylphosphorylcholine (L-alpha-GPC) was successfully prepared from phosphatidylcholine (PC) of food-grade soy lecithin powder using a novel enzymatic reaction in an aqueous medium. 94.5% yield of L-alpha-GPC was obtained under the optimal conditions of 55 degrees C, 6.67 mg/mL substrate, 2 mM CaCl2, and 33.4 U/mL phospholipase A(1) (Lecitase Ultra). L-alpha-GPC at 98% purity, 73.4% (wt%) recovery, and specific rotation ([alpha](D)(20)) of -2.5 degrees was achieved by silica gel column chromatography. Owing to its excellent catalytic efficiency, low cost, and ready availability, phospholipase A(1) (Lecitase Ultra) provides a very satisfactory option for converting PC to L-alpha-GPC. Practical applications: L-alpha-Glycerylphosphorylcholine (L-alpha-GPC) has been studied recently for its potential use as a supplement that may support neurological functions, but it is only found in trace amounts in nature. The present results indicate that Lecitase Ultra can be used for producing L-alpha-GPC from aqueous PC and suggest encouraging prospects for practical or industrial applications utilizing its notable catalytic performance, economy, and convenience.
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