Article
Biotechnology & Applied Microbiology
Elena Bancalari, Monica Gatti, Benedetta Bottari, Diego Mora, Stefania Arioli
Summary: The production of exopolysaccharides by 3 strains of Lactobacillus delbrueckii subsp. bulgaricus was evaluated using impedometric measurements, fluorescent microscopy, and flow cytometry analyses. The combined approach revealed differences in EPS production kinetics and highlighted the importance of EPS-producing cells within the same population. This study provides insights for selecting strains for dairy products where EPS production is a key feature.
Article
Chemistry, Applied
Ao Liu, Qiqi Liu, Yushan Bu, Haining Hao, Tongjie Liu, Pimin Gong, Lanwei Zhang, Chen Chen, Huaixiang Tian, Huaxi Yi
Summary: The aroma of fermented milk produced by 28 strains of Lactobacillus delbrueckii subsp. bulgaricus was evaluated and classified into different types. A total of 12 key aroma compounds were identified, which can be used to develop fermented milk products with specific aroma profiles.
Article
Food Science & Technology
Jialei Sun, Wenjing Cai, Yu Wang, Haiyue Niu, Xi Chen, Xue Han
Summary: Spray drying has lower energy costs compared to freeze-drying, but it also has the disadvantage of lower survival rate. The critical water content for spray drying Lactobacillus delbrueckii subsp. bulgaricus was found to be 21.10%, below which the survival rate decreased. Proteomic analysis revealed that differentially expressed proteins associated with cell membrane and transport were responsible for the inactivation of the bacteria during and after spray drying. Ca++/Mg++ ATPase was identified as a key protein and increasing the intracellular Ca++ or Mg++ concentration enhanced its activity, leading to increased survival of spray-dried LAB.
Article
Biochemistry & Molecular Biology
Giulia Remaggi, Benedetta Bottari, Elena Bancalari, Ovidio Catanzano, Erasmo Neviani, Lisa Elviri
Summary: Derivatives from Lactobacillus delbrueckii subsp. bulgaricus were extracted and used to produce self-crosslinking 3D printed hydrogels. These derivatives showed positive effects on human fibroblast, increasing cell proliferation and migration. The derivatives obtained from the LB1932 strain had higher efficacy, possibly due to the increase in collagen and anti-apoptotic proteins production.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Virology
Sabrina Sprotte, Olakunle Fagbemigun, Erik Brinks, Gyu-Sung Cho, Eoghan Casey, Folarin A. Oguntoyinbo, Horst Neve, Jennifer Mahony, Douwe van Sinderen, Charles M. A. P. Franz
Summary: A new Lactobacillus delbrueckii bacteriophage PMBT4 was isolated from nono and belongs to the Cequinquevirus genus. It possesses a collar-like neck passage structure and high sequence homologies to other Lb. delbrueckii phages.
Article
Food Science & Technology
Mengying Sun, Jiang Yu, Yinglong Song, Xinling Li, Guangqing Mu, Yanfeng Tuo
Summary: The metabolites produced by starter cultures have a significant impact on the taste, texture, and nutritional value of fermented milk. This study focuses on the dynamic changes of metabolites produced by Lactobacillus and Kluyveromyces marxianus during storage at 4 degrees C. The results show that the metabolic profiles of fermented milk change noticeably during storage, particularly in relation to amino acids, benzene derivatives, aldehydes, ketones, esters, and organic acids. The addition of Kluyveromyces marxianus leads to higher levels of small peptides and lactic acid in fermented milk compared to fermentation by Lactobacillus alone. Furthermore, the combination of Lactobacillus and Kluyveromyces marxianus is more efficient in metabolizing amino acids and carbohydrates than Lactobacillus alone. These findings suggest a potential symbiotic relationship between Lactobacillus, Kluyveromyces marxianus, and metabolite utilization during milk fermentation.
Article
Engineering, Chemical
Ivan Ivanov, Kaloyan Petrov, Valentin Lozanov, Iassen Hristov, Zhengjun Wu, Zhenmin Liu, Penka Petrova
Summary: This study explores the biodiversity and abundance of bioactive metabolites in Bulgarian yoghurt. The findings reveal that homemade yoghurts mainly consist of Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus, along with other accompanying lactic acid bacteria. Additionally, the yoghurts contain a high content of free essential amino acids and various bioactive compounds, indicating the potential probiotic qualities of the accompanying microflora.
Article
Construction & Building Technology
Peihua Wang, Nan Zhang, Patrick K. H. Lee, Yuguo Li
Summary: In this study, Lactobacillus delbrueckii subsp. Bulgaricus was used as a tracer organism to investigate bacterial contamination spread on environmental surfaces in an office setting. The results showed that contamination levels decreased as the physical distance from the carrier's seat location increased, indicating that L. bulgaricus can be feasibly used in large-scale field studies of bacterial contamination spread.
BUILDING AND ENVIRONMENT
(2021)
Article
Food Science & Technology
Hongji Ye, Xinyi Zhang, Yang Jiang, Min Guo, Xiaoming Liu, Jianxin Zhao, Hao Zhang, Wei Chen
Summary: This study investigated the peptidomic patterns of fermented milk by different strains of Lactobacillus delbrueckii ssp. bulgaricus and ssp. lactis before and after simulated gastrointestinal digestion. Variations in peptidomics were found among samples, with different subspecies displaying distinct peptide profiles. Digestion appeared to reduce peptidomics variations and the strain L. delbrueckii ssp. bulgaricus DQHXNS8L6 was the most efficient in generating bioactive peptides.
Article
Chemistry, Applied
Xiaoying Zhang, Yuyang Huang, Ruxin Ma, Yuqing Tang, Yang Li, Shuang Zhang
Summary: In this study, the effects of hydrolyzation with cell envelope proteinase (CEP) on the structure and biological properties of soybean protein isolate (SPI) were investigated. CEP hydrolysis resulted in the unfolding of soybean proteins and an increase in hydrophobic amino acids, leading to increased surface hydrophobicity and fluorescence of the soybean protein hydrolysates. The hydrolysates also exhibited enhanced antioxidant activity and potential biological activities such as angiotensin-converting enzyme inhibitory activity and dipeptidyl peptidase-IV inhibitory activity.
Article
Food Science & Technology
Shuang Zhang, Lihong Wu, Yichen Zhang, Jie Zhang, Hong Cheng, Jingjing Zhang, Yuexin Liu, Zimeng Kang, Lili Zhang
Summary: The purified cell-envelope proteinase (CEP) from L. bulgaricus has significant effects on the quality of fermented milk, increasing gel hardness and consistency while altering the particle size and surface charge of milk protein. CEP treatment also leads to changes in Ca2+ levels and produces fermented milk with a uniform and connected structure.
INTERNATIONAL DAIRY JOURNAL
(2023)
Article
Biotechnology & Applied Microbiology
Tamara Bendig, Andreas Ulmer, Laura Luzia, Susanne Mueller, Sven Sahle, Frank T. Bergmann, Maren Loesch, Florian Erdemann, Ahmad A. Zeidan, Sebastian N. Mendoza, Bas Teusink, Ralf Takors, Ursula Kummer, Ana Sofia Figueiredo
Summary: The fermentation process of milk to yoghurt using Lactobacillus delbrueckii subsp. bulgaricus in co-culture with Streptococcus thermophilus is characterized by lactose breakdown and acidification, which impairs metabolic activities and microbial growth. A proton-dependent computational model was developed to understand the metabolic impact of acidification, and simulation results showed a decrease in metabolic flux with acidification of the cytosol.
JOURNAL OF BIOTECHNOLOGY
(2023)
Article
Microbiology
Gabriela Christina Kuhl, Ricardo Ruiz Mazzon, Brenda Lee Simas Porto, Tamela Zamboni Madaloz, Guilherme Razzera, Daniel De Oliveira Patricio, Kevin Linehan, Grace Ahern, Harsh Mathur, Paul Ross, Catherine Stanton, Juliano De Dea Lindner
Summary: This study sheds light on the enzymatic mechanism of CLA synthesis in L. delbrueckii subsp. bulgaricus and expands our understanding of bioconversion of LA and RA by OleH. OleH's impact on the production of the c9, t11 CLA isomer and stress tolerance in E. coli has been elucidated. The study also provides insights into factors influencing OleH activity.
MICROBIOLOGY SPECTRUM
(2021)
Article
Biochemistry & Molecular Biology
Maolin Zhang, Qiaoling Lu, Jing Bai, Jinyan Gao, Zhihua Wu, Xin Li, Ping Tong, Hongbing Chen, Anshu Yang
Summary: This study aimed to investigate the intervention effects of three forms of Lactobacillus delbrueckii subsp. bulgaricus (Ld) on cell degranulation, intestinal barrier function, and intestinal mucosal immunity against soybean allergy. The results showed that both live bacteria and heat-inactivated bacteria could inhibit cell degranulation and suppress inflammation. In addition, heat-inactivated bacteria could restore the immune balance and show potential for the treatment of soybean allergy.
Article
Biotechnology & Applied Microbiology
Maria de L. Tovilla Coutino, Stephanie Passot, Ioan-Cristian Trelea, Marie-Helene Ropers, Yann Gohon, Fernanda Fonseca
Summary: This study investigates the effects of fermentation parameters on the productivity and resistance of Lactobacillus delbrueckii subsp. bulgaricus CFL1 to freezing, freeze-drying, and freeze-dried storage. Specific acidifying activity was determined after different cultivation conditions and multiple regression analyses were performed to identify the effects of these parameters on activity losses. The results showed that temperature and pH during growth influenced biomass productivity and resistance differently, and there was no single fermentation condition that improved both aspects.
JOURNAL OF APPLIED MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Abid Ali Khaskheli, Farah N. Talpur, Muhammad Aqeel Ashraf, Aysun Cebeci, Sana Jawaid, Hassan Imran Afridi
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
(2015)
Article
Biochemical Research Methods
Kurtulus Gokduman, M. Dilek Avsaroglu, Aris Cakiris, Duran Ustek, G. Candan Gurakan
JOURNAL OF MICROBIOLOGICAL METHODS
(2016)
Article
Multidisciplinary Sciences
Naniye Malli Cetinbas, Jessica Sudderth, Robert C. Harris, Aysun Cebeci, Gian L. Negri, Oemer H. Yilmaz, Ralph J. DeBerardinis, Poul H. Sorensen
SCIENTIFIC REPORTS
(2016)
Article
Biotechnology & Applied Microbiology
F. Kara, G. C. Gurakan, F. D. Sanin
BIOTECHNOLOGY AND BIOENGINEERING
(2008)
Article
Biotechnology & Applied Microbiology
Fadime Kara Murdoch, Robert W. Murdoch, Candan Gurakan, F. Dilek Sanin
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
(2018)
Article
Agriculture, Dairy & Animal Science
Aysun Cebeci, G. Candan Guerakan
JOURNAL OF DAIRY RESEARCH
(2008)
Review
Biotechnology & Applied Microbiology
Barbara Nielsen, G. Candan Gurakan, Guelhan Uenlue
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2014)
Article
Food Science & Technology
Cansu Leylak, Kuebra Sultan Ozdemir, G. Candan Gurakan, Zumrut Begum Ogel
Summary: Encapsulation of Lactobacillus acidophilus LA-5 by spray drying using whey powder and gum arabic mixtures was optimized using the BBD method, resulting in maximum encapsulation efficiency and production efficiency. Microcapsules produced under optimum conditions provided better protection of L. acidophilus after exposure to simulated gastrointestinal conditions. The particle size of the microcapsules was suitable for incorporation into dairy and other food products.
INTERNATIONAL DAIRY JOURNAL
(2021)
Article
Biotechnology & Applied Microbiology
G. Candan Gurakan, Ipek Aktuna, Elnaz Seyedmonir
Summary: This study investigated the diversity of wild yeast in traditional Turkish wine. The identified yeast species in Turkish grape wines were compared with those from other countries. Real-time PCR and DNA sequencing techniques were used to detect and identify wild yeasts from different grape varieties. A total of 469 yeast colonies were isolated and identified, and nine non-Saccharomyces yeast species belonging to seven genera were identified. The study confirmed the accuracy of real-time PCR and constructed a phylogenetic tree for non-Saccharomyces yeast species.
AMERICAN JOURNAL OF ENOLOGY AND VITICULTURE
(2022)
Article
Pharmacology & Pharmacy
Erkin Aydin, Aysun Cebeci, Ayca Lekesizcan
Summary: This study developed chitosan/siRNA nanoparticles that effectively reduced cisplatin-induced nephrotoxicity, providing significant protection to the kidneys.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Article
Biotechnology & Applied Microbiology
Abid Ali Khaskheli, Farah N. Talpur, Ayhan S. Demir, Aysun Cebeci, Sana Jawaid
BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY
(2013)
Article
Biotechnology & Applied Microbiology
A Cebeci, C Gürakan