Article
Parasitology
Juri Kim, Eun-Ah Park, Mee Young Shin, Soon-Jung Park
Summary: This study confirms the transcriptional activation function of GlMyb2 during encystation in Giardia lamblia. Through chromatin immunoprecipitation experiments and quantitative real-time PCR, several target genes of GlMyb2 were identified, with a detailed analysis on the promoter region of glcwp1 gene.
PARASITES & VECTORS
(2022)
Article
Cell Biology
Andrew G. Wang, Minjun Son, Emma Kenna, Nicholas Thom, Savas Tay
Summary: This study systematically profiles the NF-KB response to different signal sequences using high-throughput microfluidic live-cell analysis. The results show that the NF-KB dynamics store the short-term history of received signals and encode information about the identity and dose of the prior stimulus. This research provides insights into how signal transduction networks process inflammatory stimuli to coordinate cellular responses.
Article
Biochemistry & Molecular Biology
Lautaro Natali, Gabriel Luna Pizarro, Sofia Moyano, Benjamin de la Cruz-Thea, Juliana Musso, Andrea S. Ropolo, Norbert Eichner, Gunter Meister, Melina M. Musri, Constanza Feliziani, Maria C. Touz
Summary: The genetically related assemblages of the intestinal protozoa parasite Giardia lamblia are morphologically indistinguishable and are often derived from specific hosts. RNA sequencing analysis revealed that the exosomal-like vesicles (ElVs) of each assemblage contained distinct small RNA (sRNA) biotypes. These sRNAs may play a regulatory role in parasite communication and contribute to host-specificity and pathogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Rachasak Boonhok, Suthinee Sangkanu, Julalak Chuprom, Mayuna Srisuphanunt, Roghayeh Norouzi, Abolghasem Siyadatpanah, Farzaneh Mirzaei, Watcharapong Mitsuwan, Sueptrakool Wisessombat, Maria de Lourdes Pereira, Mohammed Rahmatullah, Polrat Wilairatana, Christophe Wiart, Lim Chooi Ling, Karma G. Dolma, Veeranoot Nissapatorn
Summary: The study revealed the anti-parasitic activity of P. harmala seed extract against Acanthamoeba triangularis, inhibiting cyst formation and inducing vacuolization in trophozoites. Molecular analysis indicated fluctuations in the expression levels of certain autophagy-related genes during treatment.
Article
Medicine, Research & Experimental
Itzel Reyes-Duarte, Alexel J. Burgara-Estrella, Lorena Bustamante-Cordova, Silvia Y. Moya-Camarena, Hector Parra-Sanchez, Osiris Alvarez-Bajo, Guillermo Lopez-Cervantes, Maricela Montalvo-Corral
Summary: This study found that conjugated linoleic acid (CLA) can improve mucosal immune function, inhibit Giardia lamblia infection, and promote early antibody production, thus restoring intestinal balance after infection.
IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES
(2022)
Article
Public, Environmental & Occupational Health
Gedeon Prince Manouana, Natalie Byrne, Mirabeau Mbong Ngwese, Alvyn Nguema Moure, Philipp Hofmann, Gedeon Bingoulou Matsougou, Fabrice Lotola Mougeni, Elsy Nnoh Dansou, Maradona Daouda Agbanrin, Christiane Sidonie Mapikou Gouleu, Simon Ategbo, Jeannot Frejus Zinsou, Bayode Romeo Adegbite, Jean Ronald Edoa, Peter Gottfried Kremsner, Benjamin Mordmueller, Daniel Eibach, Matthew McCall, Alabi Abraham, Steffen Borrmann, Ayola Akim Adegnika
Summary: In a observational study conducted in Lambarene, Gabon, at least one infectious agent was found in 50% of samples collected from children under 5 with diarrhea. Enteroinvasive E. coli/Shigella and enterotoxigenic E. coli were the most frequently isolated pathogens. Coinfection with multiple pathogens was also common, with EIEC/Shigella, ETEC, and Cryptosporidium spp. being the most frequently identified.
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
(2021)
Article
Biology
Lucy Lancaster, Harshil Patel, Gavin Kelly, Frank Uhlmann
Summary: This study used rapid conditional condensin depletion in budding yeast to investigate its role in transcriptional regulation. The results suggest that condensin may affect gene regulation through local effects on gene expression, as well as facilitating transcriptional reprogramming in response to environmental stimuli.
LIFE SCIENCE ALLIANCE
(2021)
Article
Biochemistry & Molecular Biology
Qingji Lyu, Qianying Yang, Jia Hao, Yuan Yue, Xiaodong Wang, Jianhui Tian, Lei An
Summary: Females have two X chromosomes and males have only one in most mammals. X chromosome inactivation (XCI) occurs in females to equalize X-dosage between sexes. Besides, mammals also balance the dosage between X chromosomes and autosomes via X chromosome upregulation (XCU) to fine-tune X-linked expression and thus maintain genomic homeostasis. Using mouse preimplantation embryos as the model, researchers found that XCU initially occurs upon major zygotic genome activation and co-regulates X-linked expression in cooperation with imprinted XCI during preimplantation development. They also identified that bromodomain containing 4 (BRD4) plays a key role in the transcription activation of XCU during preimplantation development. The findings advance our understanding of X-dosage compensation, provide a mechanism for XCU initiation, and offer important insights into the developmental and pathological role of XCU.
Article
Pediatrics
Shamsun Nahar Shaima, Sumon Kumar Das, Shahnawaz Ahmed, Yasmin Jahan, Soroar Hossain Khan, Gazi Md. Salahuddin Mamun, Abu Sadat Mohammad Sayeem Bin Shahid, Irin Parvin, Tahmeed Ahmed, A. S. G. Faruque, Mohammod Jobayer Chisti
Summary: The study found a significant association between symptomatic giardiasis and worsening of nutritional status in children under five, with asymptomatic giardia infection rates of 21.7% and 30.7% in Asian and African regions, respectively. Symptomatic giardiasis was more frequently associated with wasting and underweight.
Article
Medicine, General & Internal
Ahmed Elrefaey, Anza B. Memon
Summary: Sensory neuronopathy is a rare disorder characterized by early-onset ataxia and non-length-dependent sensory deficits. While diabetes is the most common cause of length-dependent neuropathy, other conditions such as autoimmune diseases and viral infections can also be common etiologies in acute presentations. This report presents a case of sensory neuronopathy following Giardia infection, with improvement in symptoms after monthly IVIG treatment.
CUREUS JOURNAL OF MEDICAL SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Justyna A. Resztak, Julong Wei, Samuele Zilioli, Edward Sendler, Adnan Alazizi, Henriette E. Mair-Meijers, Peijun Wu, Xiaoquan Wen, Richard B. Slatcher, Xiang Zhou, Francesca Luca, Roger Pique-Regi
Summary: This study used single-cell RNA-seq to investigate the dynamics of the transcriptional response to glucocorticoids in activated peripheral blood mononuclear cells. The results showed that glucocorticoids reversed the effects of immune stimulation on gene expression and identified genetic variants regulating gene expression and variability.
Article
Biochemistry & Molecular Biology
Zhi Chai, Yafei Lyu, Qiuyan Chen, Cheng-Hsin Wei, Lindsay M. Snyder, Veronika Weaver, Aswathy Sebastian, Istvan Albert, Qunhua Li, Margherita T. Cantorna, A. Catharine Ross
Summary: This study investigated the impact of preexisting vitamin A deficiency on gene expression patterns in the intestines using a mouse model, and identified novel target genes in vitamin A-related biological pathways. The results revealed significant effects of vitamin A deficiency on gene expression patterns in the small intestine, suggesting potential new candidate genes, while in the colon, cell division was the only enriched category negatively associated with vitamin A.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Shani Hadar, Anatoly Meller, Naseeb Saida, Reut Shalgi
Summary: Exposure to certain stresses leads to readthrough transcription, which has an important impact on cellular stress response. This study found that readthrough transcription can extend into downstream genes, and these read-in genes have common genomic characteristics, such as GC-richness and short length. Moreover, read-in genes show significant intron retention, which is not solely related to their intron length and GC content. Additionally, H3K36me3 is enriched upstream of read-in genes.
Article
Multidisciplinary Sciences
Rachael M. Zemek, Wee Loong Chin, Vanessa S. Fear, Ben Wylie, Thomas H. Casey, Cath Forbes, Caitlin M. Tilsed, Louis Boon, Belinda B. Guo, Anthony Bosco, Alistair R. R. Forrest, Michael J. Millward, Anna K. Nowak, Richard A. Lake, Timo Lassmann, W. Joost Lesterhuis
Summary: The biological determinants of the response to immune checkpoint blockade (ICB) in cancer are not fully understood. This study shows that responding tumors are associated with transient IFN-beta signaling, which can promote the anti-tumor response. The authors also identify inflammatory monocytes as the primary source of IFN beta and find that active type-I-IFN signaling in tumor-infiltrating inflammatory monocytes is associated with T cell expansion in patients treated with ICB.
NATURE COMMUNICATIONS
(2022)
Article
Immunology
Assunta Liberti, Carla Pollastro, Gabriella Pinto, Anna Illiano, Rita Marino, Angela Amoresano, Antonietta Spagnuolo, Paolo Sordino
Summary: This study investigated the inflammatory response induced by bacterial and fungal stimuli in Ciona juveniles at stage 4 of metamorphosis using transcriptional and proteomic approaches. It was found that specific inflammatory mechanisms were triggered by bacterial stimuli (Pam2CSK4 and zymosan), while bacterial LPS did not induce a significant response.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Alejandro Jimenez-Melendez, Chandra Ramakrishnan, Adrian B. Hehl, Giancarlo Russo, Gema Alvarez-Garcia
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2020)
Article
Multidisciplinary Sciences
Sean Walkowiak, Liangliang Gao, Cecile Monat, Georg Haberer, Mulualem T. Kassa, Jemima Brinton, Ricardo H. Ramirez-Gonzalez, Markus C. Kolodziej, Emily Delorean, Dinushika Thambugala, Valentyna Klymiuk, Brook Byrns, Heidrun Gundlach, Venkat Bandi, Jorge Nunez Siri, Kirby Nilsen, Catharine Aquino, Axel Himmelbach, Dario Copetti, Tomohiro Ban, Luca Venturini, Michael Bevan, Bernardo Clavijo, Dal-Hoe Koo, Jennifer Ens, Krystalee Wiebe, Amidou N'Diaye, Allen K. Fritz, Carl Gutwin, Anne Fiebig, Christine Fosker, Bin Xiao Fu, Gonzalo Garcia Accinelli, Keith A. Gardner, Nick Fradgley, Juan Gutierrez-Gonzalez, Gwyneth Halstead-Nussloch, Masaomi Hatakeyama, Chu Shin Koh, Jasline Deek, Alejandro C. Costamagna, Pierre Fobert, Darren Heavens, Hiroyuki Kanamori, Kanako Kawaura, Fuminori Kobayashi, Ksenia Krasileva, Tony Kuo, Neil McKenzie, Kazuki Murata, Yusuke Nabeka, Timothy Paape, Sudharsan Padmarasu, Lawrence Percival-Alwyn, Sateesh Kagale, Uwe Scholz, Jun Sese, Philomin Juliana, Ravi Singh, Rie Shimizu-Inatsugi, David Swarbreck, James Cockram, Hikmet Budak, Toshiaki Tameshige, Tsuyoshi Tanaka, Hiroyuki Tsuji, Jonathan Wright, Jianzhong Wu, Burkhard Steuernagel, Ian Small, Sylvie Cloutier, Gabriel Keeble-Gagnere, Gary Muehlbauer, Josquin Tibbets, Shuhei Nasuda, Joanna Melonek, Pierre J. Hucl, Andrew G. Sharpe, Matthew Clark, Erik Legg, Arvind Bharti, Peter Langridge, Anthony Hall, Cristobal Uauy, Martin Mascher, Simon G. Krattinger, Hirokazu Handa, Kentaro K. Shimizu, Assaf Distelfeld, Ken Chalmers, Beat Keller, Klaus F. X. Mayer, Jesse Poland, Nils Stein, Curt A. McCartney, Manuel Spannagl, Thomas Wicker, Curtis J. Pozniak
Article
Chemistry, Multidisciplinary
Lena Voith von Voithenberg, Aditya Kashyap, Lennart Opitz, Catharine Aquino, Timothy Sykes, Maike Nieser, Lorenzo F. T. Petrini, Nadia Enrriquez Casimiro, Xander F. van Kooten, Saskia Biskup, Ralph Schlapbach, Peter Schraml, Govind V. Kaigala
Summary: The study utilizes a modular microfluidic technology to recover cells at the microscale from tumor tissue sections, tailored to evaluate intratumoral genomic heterogeneity. The results demonstrate the importance of understanding tumor progression and heterogeneity through spatial genomic profiling.
Article
Biology
Shweta Pipaliya, Rui Santos, Dayana Salas-Leiva, Erina A. Balmer, Corina D. Wirdnam, Andrew J. Roger, Adrian B. Hehl, Carmen Faso, Joel B. Dacks
Summary: Comparing a parasitic lineage to its free-living relatives can help understand the evolutionary transition to parasitism. Research on Giardia intestinalis revealed a more varied and complete ESCRT machinery than previously thought, associated with multiple cellular locations, with changes in ESCRT complement predating adoption of a parasitic lifestyle.
Article
Plant Sciences
Kyle A. Capistrant-Fossa, Hilary G. Morrison, Aschwin H. Engelen, Charlotte T. C. Quigley, Aleksey Morozov, Ester A. Serrao, Juliet Brodie, Claire M. M. Gachon, Yacine Badis, Ladd E. Johnson, Galice Hoarau, Maria Helena Abreu, Patricia A. Tester, Leigh A. Stearns, Susan H. Brawley
Summary: The research found distinctive latitudinal distributions of microbiomes associated with Fucus vesiculosus, driven by specific ASVs that were more abundant in cold temperate to subarctic or warm temperate latitudes. These distributions correlated with humidity, tidal heights, and air/sea temperatures, but not with irradiance or photoperiod. The study also revealed novel phylogenetic biodiversity in potentially symbiotic genera with differential distributions among tissues and regions, suggesting a combination of past and contemporary environmental drivers influencing the cross-Atlantic microbial structure of F. vesiculosus.
JOURNAL OF PHYCOLOGY
(2021)
Article
Multidisciplinary Sciences
Gaelle Lentini, Rouaa Ben Chaabene, Oscar Vadas, Chandra Ramakrishnan, Budhaditya Mukherjee, Ved Mehta, Matteo Lunghi, Jonas Grossmann, Bohumil Maco, Remy Visentin, Adrian B. Hehl, Volodymyr M. Korkhov, Dominique Soldati-Favre
Summary: The study identifies a rhoptry-resident kinase RON13 as a key virulence factor that controls host cell invasion by anchoring the moving junction at the parasite-host cell interface. It shows that RON13 phosphorylates the components of the RON complex, providing insights into the mechanism of Toxoplasma gondii invasion.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Florian Trigodet, Karen Lolans, Emily Fogarty, Alon Shaiber, Hilary G. Morrison, Luis Barreiro, Bana Jabri, A. Murat Eren
Summary: Long-read sequencing strategies offer comprehensive insights into genetic information organization in genomes and metagenomes by providing extremely long contiguous characterization of individual DNA molecules. However, successful long-read sequencing experiments require high concentrations of highly purified high molecular weight (HMW) DNA. This study compares six HMW DNA extraction strategies using commercially available kits, phenol-chloroform (PC) extraction and agarose encasement followed by agarase digestion with human tongue scrapings. The results suggest that column-based kits with enzyme supplementation may be more appropriate than PC methods for long-read sequencing of metagenomes.
MOLECULAR ECOLOGY RESOURCES
(2022)
Editorial Material
Cell Biology
Carmen Faso, Adrian B. Hehl
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Microbiology
Katharina Jahn, David Dreifuss, Ivan Topolsky, Anina Kull, Pravin Ganesanandamoorthy, Xavier Fernandez-Cassi, Carola Banziger, Alexander J. Devaux, Elyse Stachler, Lea Caduff, Federica Cariti, Alex Tunas Corzon, Lara Fuhrmann, Chaoran Chen, Kim Philipp Jablonski, Sarah Nadeau, Mirjam Feldkamp, Christian Beisel, Catharine Aquino, Tanja Stadler, Christoph Ort, Tamar Kohn, Timothy R. Julian, Niko Beerenwinkel
Summary: Genomic sequencing of wastewater samples can provide early detection and surveillance of SARS-CoV-2 variants. The COJAC bioinformatics method based on variant-specific signature mutations is a robust indicator of low-frequency variants. Analysis of multiple wastewater samples allows for estimation of variant prevalence and transmission fitness advantage.
NATURE MICROBIOLOGY
(2022)
Article
Biology
Rui Santos, Asgeir Astvaldsson, Shweta Pipaliya, Jon Paulin Zumthor, Joel B. Dacks, Staffan Svard, Adrian B. Hehl, Carmen Faso
Summary: This study presents a quantitative morphological characterization of Giardia lamblia's endocytic system using volumetric electron microscopy and super-resolution microscopy. The study also compared endosome-like organelles in phylogenetically related taxa. The findings shed light on the evolution of GlACLC analogues in the Fornicata supergroup and their possible role in the formation and maintenance of stable clathrin assemblies at PVs in Giardia.
Article
Biochemistry & Molecular Biology
Sunil Kumar Dogga, Matteo Lunghi, Bohumil Maco, Jiagui Li, Beatrice Claudi, Jean-Baptiste Marq, Natalia Chicherova, Tobias Kockmann, Dirk Bumann, Adrian B. Hehl, Dominique Soldati-Favre, Pierre-Mehdi Hammoudi
Summary: Virulence and persistence of Toxoplasma gondii involve the secretion and cleavage of effector proteins by the aspartyl protease 5 (ASP5), which play important roles in host defense modulation and cyst wall formation. The absence of ASP5 impairs cyst wall formation and reduces cyst burden in chronically infected animals.
MOLECULAR MICROBIOLOGY
(2022)
Article
Microbiology
Chandra Ramakrishnan, Aarti Krishnan, Samuel Francisco, Marc W. Schmid, Giancarlo Russo, Alexandre Leitao, Andrew Hemphill, Dominique Soldati-Favre, Adrian B. Hehl
Summary: This study presents a comparative analysis of gene expression in different stages of the Besnoitia besnoiti parasite, and discovers its potential as a model organism for studying the evolution of tissue cysts and the biology of related parasites. This research contributes to a deeper understanding of the biology of besnoitiosis in cattle and other tissue-cyst forming parasites.
Article
Infectious Diseases
Thi Thuy Nguyen, Ketsarin Kamyingkird, Rungrot Jam-on, Waraphon Phimpraphai, Pun Panomwan, Adrian B. Hehl, Tawin Inpankaew
Summary: Toxoplasmosis is a common zoonotic disease in the duck meat industry in Thailand, posing risks to the food chain of humans and domestic animals. Ducks are vulnerable to infection due to their feeding habits. This study examined the prevalence of Toxoplasma gondii infection in free-grazing ducks and highlighted the need for the One Health approach to manage the risks.
TROPICAL MEDICINE AND INFECTIOUS DISEASE
(2023)
Article
Microbiology
Naomi Morrissette, Izra Abbaali, Chandra Ramakrishnan, Adrian B. Hehl
Summary: Using bioinformatics, we analyzed tubulin features in Apicomplexa, a phylum of protozoan parasites. These parasites have one to four genes for alpha- and beta-tubulin isotypes, which may have redundant or specialized functions. Some Apicomplexa also have genes for delta- and epsilon-tubulins, found in organisms with appendage-containing basal bodies. The importance of delta- and epsilon-tubulins in Apicomplexa seems limited to microgametes, with reduced requirements for centrioles, basal bodies, and axonemes in other species.
Article
Microbiology
Reilly R. Dibner, A. Monique Weaver, Marcus T. Brock, Gordon F. Custer, Hilary G. Morrison, Lois Maignien, Cynthia Weinig
Summary: Elapsed time strongly predicts microbial diversity, with few differences among developmental stages. This suggests that time and microbial succession likely have a stronger impact on microbial community assembly than host developmental stage.
FEMS MICROBIOLOGY ECOLOGY
(2021)
