Article
Chemistry, Multidisciplinary
Farhana Iylia Fatinee Mohd Yusree, Angela Paul Peter, Nurul Ainina Binti Zulkifli, Mohd Zuhair Mohd Nor, Mohd Salahuddin Mohd Basri, Mohd Noriznan Mokhtar, Mukesh Kumar Awasthi, Pau Loke Show
Summary: Liquid biphasic system (LBS) is a promising green extraction technology for the recovery of beta-amylase from sweet potato slurry. The optimized LBS showed improved recovery yield and purification factor for beta-amylase.
SUSTAINABLE CHEMISTRY AND PHARMACY
(2022)
Article
Chemistry, Applied
PR. Amulya, Rayees ul Islam
Summary: Enzyme-assisted extraction was optimized for total yield, total anthocyanin content, antioxidant activity and total phenolic content from eggplant peel using response surface methodology. The optimized extraction parameters were found to be temperature (37.32 degrees C), enzyme concentration (5%) and extraction time (1 h), which resulted in optimal values for the aforementioned parameters.
Article
Engineering, Chemical
Grazielle Nathia-Neves, Adina L. Santana, Juliane Vigano, Julian Martinez, Maria Angela A. Meireles
Summary: Ultrasound-assisted extraction (UAE) was used to extract iridoids genipin and geniposide from semi-defatted unripe genipap. Water extraction resulted in the highest overall yield and genipin content, while ethanol extraction led to higher geniposide content. These extracts showed potential as natural food colorants.
Article
Acoustics
Wei Fan, Hanyi Duan, Xiaolan Ren, Xiaoyan Guo, Yachao Zhang, Jisheng Li, Fengying Zhang, Juan Chen, Xue Yang
Summary: The mulberry leaf protein extracted by ultrasound-assisted cellulase degradation method showed good functional characteristics and solubility under optimized conditions, making it suitable for applications in the food industry.
ULTRASONICS SONOCHEMISTRY
(2023)
Article
Physics, Applied
Kevin H. R. Rouwenhorst, Leon Lefferts
Summary: This article mainly introduces in situ product removal as a method to improve the energy efficiency and product yield of plasma-based conversions. After summarizing the advantages of in situ separations in thermal-catalytic processes, different strategies for in situ product removal for plasma-based conversions are discussed. The advantages and challenges of four strategies, including absorption and adsorption using solids, use of sacrificial reactants, separation with ceramic dense membranes, and product removal in the liquid phase, are discussed.
PLASMA PROCESSES AND POLYMERS
(2023)
Article
Agricultural Engineering
Fanyan Zeng, Shanshan Wang, Jiarui Liang, Liming Cao, Xiaoxu Liu, Chengrong Qin, Chen Liang, Chuanling Si, Zebin Yu, Shuangquan Yao
Summary: In this study, the separation of bamboo hemicellulose was successfully achieved via a one-step freeze-thaw-assisted alkali treatment (OFT/AT). The selectivity of hemicellulose separation was improved, leading to a higher separation yield compared to conventional freeze-thaw-assisted alkaline treatment (FT/AT). The inhibition of lignin and cellulose separation was observed as the alkali concentration decreased, while the extraction yield of hemicellulose increased. Structural analysis revealed that the extracted hemicellulose had a relatively complete molecular structure, providing theoretical support for efficient hemicellulose separation by alkali treatment.
BIORESOURCE TECHNOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Ulises A. Salas-Villalobos, Rigel Gomez-Acata, Josefina Castillo-Reyna, Oscar Aguilar
Summary: Microbial growth inhibition by end-products affects yields and productivity of fermentative bioprocesses. In situ extraction techniques integrated at the bioreactor stage offer an attractive solution to increase titers and reduce downstream operations, thereby improving overall process efficiency.Various experimental strategies for in situ product recovery, from classical to hybrid techniques, are discussed with their pros and cons, providing a general overview for selection criteria in different bioproducts/bioprocesses.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jiyeon Hong, Dahun Jung, Saerom Park, Yujin Oh, Kyeong Keun Oh, Sang Hyun Lee
Summary: Genipin, a non-toxic natural cross-linker, was successfully used to prepare cross-linked enzyme aggregates (CLEAs) with high thermal and pH stability, nano-sized, and maintained 85% of initial activity after 10 cycles of reuse. This alternative to toxic compounds like glutaraldehyde shows promising potential for industrial applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Chemistry, Multidisciplinary
Hai-Bing Zhao, Jia-Nan Huang, Qi Qin, Hong-Yan Chen, Dai-Bin Kuang
Summary: A CsCuCl3/Cu nanocrystals heterojunction catalyst was synthesized through a facile acid-etching solution process, which exhibited excellent charge separation efficiency and improved CO2 adsorption and activation, leading to high electron consumption rate and CH4 selectivity.
Review
Chemistry, Analytical
K. K. Asanka Sanjeewa, K. H. I. N. M. Herath, Young-Sang Kim, You-Jin Jeon, Se-Kwon Kim
Summary: Identifying and characterizing bioactive compounds from natural sources is crucial for many industries. Advanced extraction techniques, including enzyme-assisted extraction, play a unique role in efficiently recovering these compounds. The efficient extraction of bioactive compounds from marine organisms, such as seaweeds and microalgae, holds potential benefits for the development of functional products.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Polymer Science
Yaqiong Wang, Haiyan Pan, Zhaowei Meng, Cai Zhang
Summary: The study aims to develop a biocompatible photothermal agent with tumor enrichment, inspired by parasitized snails, using alginate-calcium-genipin (ACG) hydrogel for photothermal therapy.
Article
Acoustics
Zhenjie Tang, Gangliang Huang, Hualiang Huang
Summary: Response surface methodology was used to explore the optimal conditions for ultrasonic-assisted cellulase extraction of Garcinia mangostana rind polysaccharides (GMRPs), and carboxymethylation was conducted to modify GMRPs. The study found that the amount of chloroacetic acid significantly affected the degree of substitution of carboxymethylated derivatives.
ULTRASONICS SONOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Tyler R. Priddy-Arrington, Reagan E. Edwards, Claire E. Colley, Marissa M. Nguyen, Tess Hamilton-Adaire, Mary E. Caldorera-Moore
Summary: Recently, there has been a growing interest in injectable, in situ crosslinking hydrogels that can conform to their environment with minimal invasion. Current in situ crosslinking chitosan hydrogels either lack biocompatibility and degrade slowly due to toxic crosslinking agents, or are mechanically weak and degrade rapidly due to insufficient crosslinking. In this study, the authors developed a thermally-driven, injectable chitosan-genipin hydrogel that can be crosslinked at 37 degrees C, which demonstrates mechanical strength, biodegradability, and high biocompatibility. The natural crosslinker used is genipin, which is non-toxic and activated by temperature. The characteristics of the chitosan-genipin hydrogel, such as crosslinking kinetics, injectability, viscoelasticity, swelling and pH response, and biocompatibility with keratinocyte cells, were analyzed. The results showed that the hydrogel can be successfully crosslinked at 37 degrees C, and it remains mechanically stable while being biodegradable. Long-term cell viability tests demonstrated excellent biocompatibility of the chitosan-genipin hydrogel, even during the crosslinking phase. Overall, these findings support the development of an injectable, in situ crosslinking chitosan-genipin hydrogel for minimally invasive biomedical applications.
MACROMOLECULAR BIOSCIENCE
(2023)
Article
Engineering, Chemical
David Speer, Tirth Patel, Brian Ho, Jack Phillips, Tianyang Zhu, Joseph Shangraw, Meltem Urgun-Demirtas, Yanna Liang, Anish Tuteja
Summary: In 2018, the US landfilleds 35.3 million tons of food waste, accounting for 24% of the total landfill mass. Some states have started to ban or discourage the disposal of food waste and sewage sludge into landfills due to their negative impacts on the environment and human health. This has created an urgent need for the development of digestion processes to convert food waste into valuable chemical products.
SEPARATION AND PURIFICATION TECHNOLOGY
(2023)
Article
Chemistry, Applied
Maria Jose Aliano Gonzalez, Ceferino Carrera, Gerardo F. Barbero, Miguel Palma
Summary: Two anthocyanin extraction methods from blackcurrant samples based on Ultrasound-Assisted Extraction (UAE) and Enzyme-Assisted Extraction (EAE) have been developed and optimized. The composition of the extraction solvent and extraction time are the most influential variables for both methods. Both methods have been proven suitable for quality control analysis of blackcurrant-derived products.
Article
Biochemistry & Molecular Biology
Chopaka Thongbamrer, Wanlapa Roobsoong, Jetsumon Sattabongkot, Praneet Opanasopit, Boon-ek Yingyongnarongkul
Summary: In this study, a series of L-shaped spermine-based cationic lipids with different hydrophobic chain lengths were designed and synthesized. The lipids with nonidentical hydrocarbon chains showed higher transfection efficiency compared to the identical analogues, and exhibited good transfection performance in different cell lines.
Review
Pharmacology & Pharmacy
Supusson Pengnam, Samarwadee Plianwong, Boon-ek Yingyongnarongkul, Prasopchai Patrojanasophon, Praneet Opanasopit
Summary: siRNA therapy can increase the deposition of chemotherapeutic drugs at the tumor site by suppressing the expression of multidrug-resistant genes. Nonviral delivery systems such as nanovesicles can improve siRNA delivery and have shown great potential in cancer therapy.
DRUG METABOLISM AND PHARMACOKINETICS
(2022)
Article
Engineering, Biomedical
Wipada Samprasit, Praneet Opanasopit, Benchawan Chamsai
Summary: The study developed dual-drugs-loaded nanoparticles for colon-specific delivery and synergistic activity against colon cancer cells. The nanoparticles showed enhanced activity and efficiency when both drugs were loaded, indicating potential for targeted nanomedicines in colon cancer treatment.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
(2022)
Article
Pharmacology & Pharmacy
Taraswi Mitra Ghosh, Teeratas Kansom, Suman Mazumder, Joshua Davis, Ahmed S. Alnaim, Shanese L. Jasper, Chu Zhang, Aedan Bird, Praneet Opanasopit, Amit K. Mitra, Robert D. Arnold
Summary: The study found that the andrographolide analog 3A.1 has anti-tumor activity against mCRPC, either alone or in combination with taxanes, showing synergistic effects. Co-treatment of 3A.1 with taxanes reduced the required dose and regulated key factors such as heat shock proteins and MAT2A to exert its effects.
JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
(2022)
Article
Pharmacology & Pharmacy
Yin Yin Myat, Tanasait Ngawhirunpat, Theerasak Rojanarata, Praneet Opanasopit, Mark Bradley, Prasopchai Patrojanasophon, Chaiyakarn Pornpitchanarong
Summary: This study synthesized PEGDA/AA nanoparticles for the delivery of Dox to colorectal cancer cells. The nanoparticles demonstrated good physical properties, drug loading and release capabilities, non-toxicity to intestinal epithelial cells, and effective killing of colorectal cancer cells. These nanoparticles show potential as a drug delivery carrier for targeted, staged-release therapy for colon cancer.
Article
Pharmacology & Pharmacy
Phuvamin Suriyaamporn, Praneet Opanasopit, Worranan Rangsimawong, Tanasait Ngawhirunpat
Summary: This study designed and developed optimal two-layered dissolving microneedles (MNs) loaded with fluconazole-microemulsions (MEs) for the potential treatment of fungal eye infection. The optimal formulation of MNs exhibited good physical properties, complete insertion, minimally invasive ocular tissue, and high stability, while showing significantly higher FLUZ permeation into the ocular tissue and providing potential antifungal activity.
Article
Pharmacology & Pharmacy
Supusson Pengnam, Purin Charoensuksai, Boon-ek Yingyongnarongkul, Rungnapha Saeeng, Hasan Uludag, Prasopchai Patrojanasophon, Praneet Opanasopit, Samarwadee Plianwong
Summary: Breast cancer is the second leading cause of cancer-related death in the US. This study developed a chitosan derivative-stabilized 3nAG nanosuspension and improved its efficacy in killing breast cancer cells by adding siRNA.
Article
Pharmacology & Pharmacy
Kwanputtha Arunprasert, Chaiyakarn Pornpitchanarong, Theerasak Rojanarata, Tanasait Ngawhirunpat, Praneet Opanasopit, Prasopchai Patrojanasophon
Summary: This research aimed to develop new hydrophilic drug-in-adhesive patches for transdermal drug delivery. The patches containing PHI-cat and HA showed considerably greater adhesion ability to human skin compared with the patches without catechol and commercial patches. Furthermore, these patches exhibited good physical and chemical stability, making them potential transdermal drug delivery systems with excellent adhesive properties for drug delivery through the skin.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Article
Pharmacology & Pharmacy
Thirapit Subongkot, Natthan Charernsriwilaiwat, Rattathammanoon Chanasongkram, Kantawat Rittem, Tanasait Ngawhirunpat, Praneet Opanasopit
Summary: This study aimed to develop microemulsions using poloxamer 124 as a surfactant to improve the skin penetration of finasteride. The microemulsions were found to significantly increase the skin penetration of finasteride, mainly through the hair follicle pathway.
Article
Pharmacology & Pharmacy
Kanokwan Singpanna, Chaiyakarn Pornpitchanarong, Prasopchai Patrojanasophon, Theerasak Rojanarata, Tanasait Ngawhirunpat, S. Kevin Li, Praneet Opanasopit
Summary: The present study evaluated the effect of gold nanoparticles stabilized by chitosan (CS-AuNPs) and citrate ions (Ci-AuNPs) on skin permeation of sodium fluorescein (NaFI) and rhodamine b base (RhB). CS-AuNPs showed enhanced permeation of NaFI, superior to Ci-AuNPs, and this enhancement was likely due to improved delivery through the transepidermal pathway. However, CS-AuNPs and Ci-AuNPs had no significant effect on the permeability of the lipophilic molecule RhB. CS-AuNPs also exhibited no cytotoxicity towards human skin fibroblast cells.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Pharmacology & Pharmacy
Thapakorn Charoenying, Praneet Opanasopit, Tanasait Ngawhirunpat, Theerasak Rojanarata, Prasert Akkaramongkolporn, Prasopchai Patrojanasophon
Summary: This study aimed to use a 3D printer to fabricate a tablet-shaped floating device for controlling the release and floating time of domperidone tablets. The device was designed with a PVA pathway to provide a predictable floating time, and the air chamber was intentionally detached to allow elimination from the body. The optimal formulation achieved a floating time of 24 hours and a controlled drug release rate.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2023)
Article
Pharmacology & Pharmacy
Benchawan Chamsai, Praneet Opanasopit, Wipada Samprasit
Summary: This study developed fast disintegrating dosage forms for oral insulin delivery by incorporating mucoadhesive thiolated chitosan (TCS)-based nano-particles (NPs). The results showed that the CD-insulin complexes were successfully encapsulated into the mucoadhesive NPs, which were promising for use in oral insulin delivery.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Pharmacology & Pharmacy
Phuvamin Suriyaamporn, Chaiyakarn Pornpitchanarong, Boonnada Pamornpathomkul, Prasopchai Patrojanasophon, Theerasak Rojanarata, Praneet Opanasopit, Tanasait Ngawhirunpat
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2023)
Article
Pharmacology & Pharmacy
Supusson Pengnam, Praneet Opanasopit, Theerasak Rojanarata, Boon-ek Yingyongnarongkul, Chopaka Thongbamrer, Samarwadee Plianwong
Summary: The challenge in HER2-overexpressing breast cancer therapy is to develop an effective target therapy. This study formulated lipid-based nanoparticles for delivering siRNA into HER2-overexpressing breast cancer cells. The dual-targeted therapy using Mcl-1 siRNA nioplex and trastuzumab showed synergistic effects in inhibiting cell growth and inducing apoptosis.
Review
Pharmacology & Pharmacy
Kanokwan Singpanna, Chaiyakarn Pornpitchanarong, Prasopchai Patrojanasophon, Theerasak Rojanarata, Tanasait Ngawhirunpat, S. Kevin Li, Praneet Opanasopit
Summary: Transdermal drug delivery via the skin is a promising method, and gold nanoparticles (AuNPs) have emerged as a useful tool in this area. This review provides a comprehensive summary of the properties, synthesis, and applications of AuNPs in transdermal drug delivery.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Thore Bach Thomsen, Tobias S. Radmer, Anne S. Meyer
Summary: Plastic pollution, particularly from PET, is a significant environmental challenge. This study investigates the enzyme kinetics of PET degradation and finds that the degree of crystallinity (X-C) has a substantial impact on the enzymatic reaction rate. The mobile amorphous fraction (X-MAF) is a better descriptor for the rate of enzymatic degradation. The longer lag phase observed with increased X-C is proposed to be due to increased substrate entanglement density and unproductive enzyme binding to the crystalline regions of PET.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)
Article
Biotechnology & Applied Microbiology
Purna Bahadur Poudel, Dipesh Dhakal, Jong Cheol Lee, Jae Kyung Sohng
Summary: Methylation plays important roles in various biological processes, and this study characterized the function of ThnM3 in regioselective modifications of 1,8-dihydroxynaphthalene.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)
Article
Biotechnology & Applied Microbiology
Feng Song, Ivan Birukou, Mark Bednarcik, Scott Young
Summary: In this study, a highly sensitive and specific method using ultra performance liquid chromatography (UPLC) was developed to measure the enzymatic activity of APH4 in plants. The method exhibited good precision and accuracy, and was validated for use with samples from different sources.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)
Article
Biotechnology & Applied Microbiology
Yuan Rao, Wei Zhuang, Jinle Liu, Ting Tang, Zhi Wang, Hanjie Ying
Summary: This study describes a mild and versatile strategy to immobilize glucose oxidase (GOD) on carboxyl-activated metal-organic framework (MOF) through DNA-directed immobilization technology. The immobilized GOD showed enhanced stability and catalytic activity, making it a potential candidate for applications in biocatalysis and biotechnology.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)
Article
Biotechnology & Applied Microbiology
Julen Ordenana Manso, Martin B. Nielsen, Eva Balaguer Moya, Juliana P. Sandri, Celina K. Yamakawa, Solange I. Mussatto
Summary: This study focuses on the development of an enzymatic cocktail for the conversion of corn fiber into monosaccharides. The results reveal that the recalcitrance and unique chemical composition of corn fiber require a tailored enzymatic cocktail for degradation, and attention should also be given to the pretreatment step.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)
Article
Biotechnology & Applied Microbiology
Cuixia Zhou, Guangcheng Yang, Panpan Meng, Weishuai Qin, Yanyan Li, Zhenxian Lin, Wei Hui, Huitu Zhang, Fuping Lu
Summary: Bacillus licheniformis 2709 is an important industrial producer of alkaline protease (AprE), and this study identified the UP elements of the aprE gene and demonstrated the lack of significant effect of CggR, FruR, and YhcZ transcription factors on aprE expression. Enhanced aprE expression and enzyme activity were achieved by deleting a negative regulatory factor binding site in the genome. These findings provide valuable insights into the high-level production of proteins in other Bacillus species.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)
Article
Biotechnology & Applied Microbiology
Xinqi Xu, Tianheng Chen, Lian Xu, Juan Lin
Summary: In this study, laccase was immobilized on modified magnetic nanoparticles, improving the enzyme's thermostability and efficiency for polymerization of phenols.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)
Article
Biotechnology & Applied Microbiology
Handan Ustukarci, Gul Ozyilmaz, Ali Tuncay Ozyilmaz
Summary: A biofilm layer called biofouling is formed over time by organic molecules and microorganisms on solid surfaces immersed in a liquid medium. This study focused on the antifouling performance of polyaniline (PANI) and investigated the immobilization of hydrolytic enzymes in PANI coatings. The results showed that the alpha-amylase-containing film exhibited the highest antifouling activity. Surface properties and biofilm composition were analyzed to support these findings.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)
Article
Biotechnology & Applied Microbiology
Mariana Ortiz-Sanchez, Juan Camilo Solarte-Toro, Pablo Jose Inocencio-Garcia, Carlos Ariel Cardona Alzate
Summary: This study evaluated the sustainability of biorefineries using orange peel as a raw material. By applying the concept of biorefineries and experimental data, it was found that steam distillation and polyphenolic compound extraction can improve the process performance, resulting in higher yields of different products. The integrated utilization of orange peel in biorefineries showed a high sustainability index.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)