期刊
ENZYME AND MICROBIAL TECHNOLOGY
卷 53, 期 2, 页码 92-96出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.enzmictec.2013.05.001
关键词
Genipin; Enzyme assisted extraction; In situ product separation; Cellulase
资金
- Thailand Research Fund (TRF) through the Royal Golden Jubilee Ph.D. Program scholarship [PHD/0093/2554]
- Faculty of Pharmacy, Silpakorn University, Thailand
A direct one-pot production of genipin, an iridoid aglycone, from crude gardenia fruit was developed. The method relied on the use of single cellulase to disrupt plant cells and to cleave off sugar molecules simultaneously, thereby enhancing the release of intracellular iridoids and converting geniposide to genipin. During the biocatalysis, eco-friendly ethyl acetate was used to extract the product, providing the partial purification and the minimization of genipin degradation. By using 10 mg/mL cellulase and 24 h-incubation at 50 degrees C, pH 4, combined with in situ extraction, genipin with good purity was yielded at 58.83 mg/g, which increased 12.38 and 1.72 times compared with those obtained from the procedures without either the aid, of enzyme or in situ extraction, respectively. Therefore, this integrated approach is promising for the production of genipin and should be potentially applied to the preparation of other plant aglycones. (C) 2013 Elsevier Inc. All rights reserved.
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