Article
Biochemistry & Molecular Biology
Alina A. Sofronova, Denis V. Pozdyshev, Kseniya V. Barinova, Vladimir I. Muronetz, Pavel I. Semenyuk
Summary: The study found that glycation of GAPDH inhibits its interaction with alpha-synuclein and RNA, potentially influencing the interplay between diabetes and neurodegenerative diseases.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2021)
Article
Chemistry, Multidisciplinary
Astrid M. Perez, Jacob A. Wolfe, Janse T. Schermerhorn, Yiwen Qian, Bekim A. Cela, Cody R. Kalinowski, Garrett E. Largoza, Peter A. Fields, Gabriel S. Brandt
Summary: This study found that the thermal stability of a critical metabolic enzyme in the stony coral Acropora millepora significantly increases in the presence of its cofactor NAD(+). The determination of the complex structure revealed variable NAD(+) occupancy across the tetrameric enzyme, with regions of difference identified that may account for the increased thermal stability.
Article
Microbiology
Shotaro Isozaki, Hiroaki Konishi, Hiroki Tanaka, Chikage Yamamura, Kentaro Moriichi, Naoki Ogawa, Mikihiro Fujiya
Summary: This study demonstrated that heptelidic acid (HA) derived from the probiotic Aspergillus oryzae exerts antitumor effects against extraintestinal melanoma cells both in vitro and in vivo. Oral administration of HA significantly suppressed the growth of transplanted melanoma tumors, indicating a potential novel system for regulating extraintestinal tumors through gut bacteria-derived bioactive mediators.
Article
Immunology
David Salcines-Cuevas, Hector Teran-Navarro, Ricardo Calderon-Gonzalez, Paula Torres-Rodriguez, Raquel Tobes, Manuel Fresno, Jorge Calvo-Montes, I. Concepcion Perez Del Molino-Bernal, Sonsoles Yanez-Diaz, Carmen Alvarez-Dominguez
Summary: The N-terminal 22 amino acid peptides of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) are highly homologous among pathogenic bacteria like Listeria, Mycobacterium, and Streptococcus, making them ideal candidates for universal vaccine designs. Vaccines containing these peptides demonstrated high immunogenicity, lack of toxicity, and cross-protection immunity in mouse models infected with different pathogens.
Article
Biochemistry & Molecular Biology
Ines Baptista, Effrosyni Karakitsou, Jean-Baptiste Cazier, Ulrich L. Guenther, Silvia Marin, Marta Cascante
Summary: Through TKTL1 knockdown experiments, it was found that TKTL1 plays a key role in the metabolic adaptation to hypoxia in THP-1 AML cells by modulating the activities of G6PD and GAPDH, which involve changes in redox capacity and transcriptional regulation of key enzymes and transporters related to glucose, PPP, and amino acid metabolism.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Sang Hyun Kim, Jeong Hyeon Hwang, Hyun Joong Kim, Suk Jin Oh, Hyun Jin Kim, Nara Shin, Sang-Hyoun Kim, Jeong-Hoon Park, Shashi Kant Bhatia, Yung-Hun Yang
Summary: The study developed Clostridium acetobutylicum as a superior biohydrogen producer by introducing glyceraldehyde-3-phosphate dehydrogenase (GAPDH) to enhance hydrogen production. The strain CAC824-G containing gapC showed a 66.3% higher hydrogen production than the wild-type strain, with increased NADH and NADPH pools. Overexpression of gapC resulted in increased hydrogen production even in the presence of inhibitors.
ENZYME AND MICROBIAL TECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Christine C. Winterbourn, Alexander Peskin, Torsten Kleffmann, Rafael Radi, Paul E. Pace
Summary: The presence of bicarbonate enhances the inactivation of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) by hydrogen peroxide (H2O2) in a reversible manner. The enhanced inactivation is most likely due to the formation of a more reactive oxidant, peroxy-monocarbonate (HCO4-). GAPDH may facilitate the formation and/or targeting of HCO4- to promote its own inactivation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Cell Biology
Xia Ding, Lihua Wang, Mingjiao Chen, Yue Wu, Shengfang Ge, Jin Li, Xianqun Fan, Ming Lin
Summary: Melanoma cells exhibit increased aerobic glycolysis, where GAPDHS plays a crucial role in regulating glycolysis. Knockdown of GAPDHS hindered glycolysis and cell proliferation, while overexpression promoted glycolysis and cell growth. SOX10 regulates the activation of GAPDHS and influences the malignant phenotype of uveal melanoma.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Ivona Pavkova, Monika Kopeckova, Marek Link, Erik Vlcak, Vlada Filimonenko, Lenka Lecova, Jitka Zakova, Pavlina Laskova, Valeria Sheshko, Miloslav Machacek, Jiri Stulik
Summary: This study discovered the involvement of Francisella tularensis GAPDH homologue GapA in multiple pathogenic processes at the host cell level. The localization of GapA was demonstrated using immunoelectron microscopy, and its interaction with multiple host cellular proteins was identified using quantitative proteomic approaches. The impact of GapA deletion on cytokine gene transcription and signaling pathway activation was investigated. The results showed that GapA enhanced the transcription of various cytokines, which was not observed in cells infected with a fully virulent wild-type strain. This study provides evidence that GapA is directly involved in multiple host cellular processes and contributes to various molecular mechanisms of pathogenesis.
Article
Parasitology
Wenhao Huang, Shunhai Zhu, Ting Chen, Qiping Zhao, Hui Dong, Bing Huang, Yawen Yao, Zhan Liu, Yu Yu, Hongyu Han
Summary: In this study, the researchers cloned and characterized the EtGAPDH gene of E. tenella and investigated its expression levels in different developmental stages and drug-resistant strains. The results showed that EtGAPDH was upregulated in drug-resistant strains, suggesting its potential involvement in the development of resistance to anticoccidial drugs.
PARASITOLOGY RESEARCH
(2022)
Article
Parasitology
Hao Jie, Si -ming Zhang, Fan-rong Ding, Chun-lian Tang, Xiang -you Li
Summary: This study evaluated the effect of SjGAPDH on the growth of schistosomula and found that SjGAPDH may affect the growth of schistosomula and could be a useful target for treating schistosomiasis.
Article
Chemistry, Physical
Adnan Ayna, Peter C. E. Moody
Summary: The study elucidates the X-ray crystal structure of Campylobacter jejuni phosphorylating glyceraldehyde 3-phosphate dehydrogenase, and investigates its role in the pathogen's metabolism.
JOURNAL OF MOLECULAR STRUCTURE
(2021)
Article
Oncology
Jennifer G. Gill, Samantha N. Leef, Vijayashree Ramesh, Misty S. Martin-Sandoval, Aparna D. Rao, Lindsey West, Sarah Muh, Wen Gu, Zhiyu Zhao, Gregory A. Hosler, Travis W. Vandergriff, Alison B. Durham, Thomas P. Mathews, Arin B. Aurora
Summary: This study characterizes metabolic changes during cancer metastasis and identifies GAPDHS as a novel regulator of these processes in melanoma cells.
Article
Plant Sciences
Cong Liu, Yanfeng Wang, Yuanyuan Du, Zhensheng Kang, Jia Guo, Jun Guo
Summary: In this study, a Pst effector protein was found to manipulate plant defense by interacting with key plant proteins. This effector protein regulated two different ROS signaling pathways by interacting with two wheat proteins, affecting the plant's resistance to Pst. Additionally, the effector protein stabilized a protein that suppresses ROS accumulation by inhibiting protein degradation. These findings revealed the mechanism behind how Pst promotes its infection by manipulating plant proteins.
PLANT CELL AND ENVIRONMENT
(2023)
Article
Biophysics
Lu-Liang Wang, Minting Fan, Xin Xing, Yushen Liu, Shuyang Sun
Summary: Immobilizing GAPDH onto Fe3O4 magnetic nanoparticles enhanced its properties in histamine degradation, shifting optimal pH and temperature and broadening pH tolerance and thermostability. The immobilized enzyme was efficient in removing histamine in fermented beverages, showing potential for practical applications.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)