Article
Biotechnology & Applied Microbiology
C. L. Bao, S. Z. Liu, Z. D. Shang, Y. J. Liu, J. Wang, W. X. Zhang, B. Dong, Y. H. Cao
Summary: The study showed that Bacillus amyloliquefaciens TL106 can effectively protect mice against EHEC O157:H7 infection by relieving inflammation, improving intestinal barrier function, mitigating permeability disruption, and stabilizing the gut microbiota.
JOURNAL OF APPLIED MICROBIOLOGY
(2021)
Article
Veterinary Sciences
Delphine Bibbal, Philippe Ruiz, Panagiotis Sapountzis, Christine Mazuy-Cruchaudet, Estelle Loukiadis, Frederic Auvray, Evelyne Forano, Hubert Brugere
Summary: This study aimed to identify farms with persistent circulation of EHEC O157:H7 in cattle. The results showed that persistent circulation may be due to the presence of a few predominant strains or repeated exposure of cattle to various strains. In addition, differences were found in the fecal microbial communities of shedders and non-shedders, with different bacterial indicators identified.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
Tao Yang, Zhenghui Wang, Yang Song, Xinyan Yang, Sihan Chen, Shiqian Fu, Xue Qin, Wei Zhang, Chaoxin Man, Yujun Jiang
Summary: A smartphone-based colorimetric aptasensor was developed for the detection of E. coli O157:H7 in milk, showing good reproducibility with no cross-reaction for other bacteria. The sensor could detect a concentration of 8.43 x 10^3 cfu/mL of E. coli O157:H7 in pure culture and 5.24 x 10^2 cfu/mL in artificially contaminated milk after 1 hour of incubation.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Chemistry, Analytical
Zhihua Li, Xue Zhang, Haishan Qi, Xiaowei Huang, Jiyong Shi, Xiaobo Zou
Summary: A renewable electrochemical biosensor based on mussel-inspired adhesive protein was developed for the detection of Escherichia coli O157:H7. The biosensor showed excellent repeatability and sensitivity, with a wide linear range and a low detection limit. It demonstrated good performance in detecting E. coli O157:H7 in seafood samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Multidisciplinary
Sarah H. Klass, Laura E. Sofen, Zachary F. Hallberg, Tahoe A. Fiala, Alexandra Ramsey, Nicholas S. Dolan, Matthew B. Francis, Ariel L. Furst
Summary: Pathogenic E. coli strains are a significant threat to public health, causing both foodborne illnesses and urinary tract infections. By using a rapid bioconjugation reaction and electrochemical impedance spectroscopy, we can selectively capture and accurately quantify these pathogenic microbes on a disposable gold electrode.
CHEMICAL COMMUNICATIONS
(2021)
Article
Food Science & Technology
Kai Dong, Yuwan Li, Sijia Luo, Fan Zhang, Hanxu Pan, Liang Zhao, Yongtao Wang, Xiaojun Liao
Summary: The study analyzed the sterilizing effects of different HHP-boosting processes on Escherichia coli O157:H7, finding that linear and fast boosting had the most significant bactericidal effects. The proteins interacting with GroEL under different boost modes and rates were involved in 42 metabolic pathways, particularly ribosome, ABC transporter, oxidative phosphorylation, sulfate assimilation, and cysteine biosynthesis. Key interacting proteins NuoB, NuoF, CysD, ZnuC, and RplX were identified and their interactions with GroEL were verified in vivo.
Article
Immunology
Juliana Gonzalez, Jimena Soledad Cadona, Claudio Marcelo Zotta, Silvina Lavayen, Roberto Vidal, Nora Lia Padola, Andrea Mariel Sanso, Ana Victoria Bustamante
Summary: This study aimed to compare the genetic diversity of VTEC O157:H7 strains isolated from human disease cases in Argentina and Chile. The results showed that these strains mainly belonged to lineage I/II, associated with hypervirulent strains, and were categorized into the phylogroup E. Additionally, genetic diversity was observed among Argentinean and Chilean strains, particularly in relation to putative virulence determinants and nle profiles.
MICROBES AND INFECTION
(2022)
Article
Microbiology
Xueqin Zhao, Lei Wang, Chunling Zhu, Xiaojing Xia, Shouping Zhang, Yimin Wang, Huihui Zhang, Yanzhao Xu, Shijun Chen, Jinqing Jiang, Shanqin Liu, Yundi Wu, Xilong Wu, Gaiping Zhang, Yueyu Bai, Hanna Fotina, Jianhe Hu
Summary: MPX demonstrated a significant therapeutic effect against E. coli infection, protecting mice from lethal infection and improving intestinal barrier function and nutrient absorption, laying the foundation for the treatment of bacterial infections.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Veterinary Sciences
Aklilu Feleke Haile, Silvia Alonso, Nega Berhe, Tizeta Bekele Atoma, Prosper N. Boyaka, Delia Grace
Summary: This study estimated the prevalence and antimicrobial resistance of Escherichia coli O157:H7 in raw beef sold in butcher shops in Addis Ababa, Ethiopia. Out of the 384 samples examined, 3.64% carried E. coli O157:H7, with 57.14% of the isolates showing resistance to three or more antimicrobial agents. Thus, stricter monitoring of antimicrobial use and further studies on contamination sources and risk mitigation strategies are needed.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Chemistry, Analytical
Manman Du, Jinbiao Ma, Zhiwei Zhang, Guangzu Wu, Jianguo Wu, Hao Wang, Xinwu Xie, Can Wang
Summary: This study developed a rGO-FET biosensor for the rapid and sensitive detection of pathogenic microorganisms. The biosensor demonstrated good specificity, a wide linear range, and the ability to directly detect real samples without pretreatment.
ANALYTICA CHIMICA ACTA
(2023)
Article
Microbiology
Aklilu Feleke Haile, Silvia Alonso, Nega Berhe, Tizeta Bekele Atoma, Prosper N. Boyaka, Delia Grace
Summary: This study examined the prevalence and antimicrobial resistance of E. coli O157:H7 in lettuce in Addis Ababa, Ethiopia. The results indicated a low prevalence but presence of drug-resistant strains, highlighting a potential public health risk associated with this pathogen in the area.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Chemistry, Analytical
Jiye Li, Jie Wang
Summary: This study demonstrates the use of extinction spectrum to quickly transform the signal of MoS2-NS aggregation and reveals the size-dependent extinction response. Additionally, the size-dependent optical extinction behavior of MoS2-NSs with aptamer-induced dispersion is studied to improve target identification ability. This approach has been successfully employed for label-free detection of Escherichia coli O157:H7.
Article
Acoustics
Jiao Li, Donghong Liu, Tian Ding
Summary: The study investigated the gene regulation at the transcription level in Escherichia coli O157:H7 after ultrasonic stimulation, revealing significant differential expressions of 1217 genes, including up-regulated and down-regulated genes related to crucial biomolecular processes. The most enriched pathways were analyzed in carbohydrate metabolism, energy metabolism, membrane transport, signal transduction, transcription, and translation categories. Additionally, the study found that the citrate cycle genes were down-regulated, affecting carbohydrate decomposition and energy production under ultrasonic stress.
ULTRASONICS SONOCHEMISTRY
(2021)
Article
Food Science & Technology
Dianbo Zhao, Shaodan Wang, Yushan Hu, Xiao Liu, Jing Tao, Gianni Sagratini, Qisen Xiang
Summary: This study reveals the antibacterial mechanism of lauric arginate (LAE) against Escherichia coli. LAE damages the cytoplasmic and outer membranes of E. coli, causing a loss of membrane integrity. It also induces oxidative stress and increases reactive oxygen species levels. The combination of membrane damage and oxidative stress contributes to the antibacterial activity of LAE.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Acoustics
Qiao He, Donghong Liu, Muthupandian Ashokkumar, Xingqian Ye, Tony Z. Jin, Mingming Guo
Summary: This study explored the response of bacterial cell membranes to ultrasound exposure. It was found that ultrasonic treatment resulted in a significant reduction in E. coli populations and increased sensitivity to antimicrobial compounds. Additionally, ultrasonic-treated cells showed membrane damages and increased membrane permeability.
ULTRASONICS SONOCHEMISTRY
(2021)
Article
Microbiology
Sophie Comtet-Marre, Frederique Chaucheyras-Durand, Ourdia Bouzid, Pascale Mosoni, Ali R. Bayat, Pierre Peyret, Evelyne Forano
FRONTIERS IN MICROBIOLOGY
(2018)
Article
Biotechnology & Applied Microbiology
Audrey Segura, Marine Bertoni, Pauline Auffret, Christophe Klopp, Olivier Bouchez, Clemence Genthon, Alexandra Durand, Yolande Bertin, Evelyne Forano
Article
Microbiology
Raphaele Gresse, Frederique Chaucheyras Durand, Lysiane Duniere, Stephanie Blanquet-Diot, Evelyne Forano
Article
Multidisciplinary Sciences
Zoe Virion, Stephane Doly, Kusumika Saha, Mireille Lambert, Francois Guillonneau, Camille Bied, Rebecca M. Duke, Pauline M. Rudd, Catherine Robbe-Masselot, Xavier Nassif, Mathieu Coureuil, Stefano Marullo
NATURE COMMUNICATIONS
(2019)
Article
Multidisciplinary Sciences
Bastien Demouveaux, Valerie Gouyer, Catherine Robbe-Masselot, Frederic Gottrand, Tetsuharu Narita, Jean-Luc Desseyn
SCIENTIFIC REPORTS
(2019)
Article
Multidisciplinary Sciences
Loic Le Guennec, Zoe Virion, Haniaa Bouzinba-Segard, Catherine Robbe-Masselot, Renaud Leonard, Xavier Nassif, Sandrine Bourdoulous, Mathieu Coureuil
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Microbiology
Mathilde Audry, Catherine Robbe-Masselot, Jean-Philippe Barnier, Benoit Gachet, Bruno Saubamea, Alain Schmitt, Sophia Schonherr-Hellec, Renaud Leonard, Xavier Nassif, Mathieu Coureuil
Article
Biochemistry & Molecular Biology
Ruifeng Bai, Xue Luan, Yu Zhang, Catherine Robbe-Masselot, Inka Brockhausen, Yin Gao
GLYCOCONJUGATE JOURNAL
(2020)
Review
Microbiology
Panagiotis Sapountzis, Audrey Segura, Mickael Desvaux, Evelyne Forano
Article
Respiratory System
Ignacio Caballero, Belinda Ringot-Destrez, Mustapha Si-Tahar, Pascal Barbry, Antoine Guillon, Isabelle Lantier, Mustapha Berri, Claire Chevaleyre, Isabelle Fleurot, Celine Barc, Reuben Ramphal, Nicolas Pons, Agnes Paquet, Kevin Lebrigand, Carole Baron, Andrea Baehr, Nikolai Klymiuk, Renaud Leonard, Catherine Robbe-Masselot
Summary: In this study, it was found that increased sialylation of CF airway mucins and impaired mucociliary transport precede inflammation onset. The absence of CFTR expression was causally related to the hypersialylation of mucins and increased adherence of P. aeruginosa to mucins. This research provides new insights into the molecular properties of mucins in CF lungs, highlighting a favorable environment for microbial development in CF airways.
JOURNAL OF CYSTIC FIBROSIS
(2021)
Article
Infectious Diseases
Raphaele Gresse, Juan J. Garrido, Angeles Jimenez-Marin, Sylvain Denis, Tom Van de Wiele, Evelyne Forano, Stephanie Blanquet-Diot, Frederique Chaucheyras-Durand
Summary: The study showed that Saccharomyces cerevisiae var boulardii could prevent post-weaning diarrhea in piglets by modulating the microbiota, reducing inflammation, and decreasing bacterial adhesion.
Article
Microbiology
Lysiane Duniere, Damien Esparteiro, Yacine Lebbaoui, Philippe Ruiz, Mickael Bernard, Agnes Thomas, Denys Durand, Evelyne Forano, Frederique Chaucheyras-Durand
Summary: In this study, it was found that in late gestating ewes, the control group showed changes in rumen and fecal microbiota and were prone to oxidative stress close to parturition. However, supplementation of live yeast alleviated these microbial changes and reduced oxidative stress.
Article
Chemistry, Multidisciplinary
Kevin Gillois, Camille Rivard, Cecile Levasseur-Garcia, Valerie Bezirard, Helene Terrisse, Renaud Leonard, Catherine Robbe-Masselot, Emmanuelle Maguin, Mathias L. Richard, Vassilia Theodorou, Marie-Helene Ropers, Muriel Mercier-Bonin, Herve Robert
Summary: This study evaluated the biodistribution and gut toxicity of two commercially available colloidal silver products, Mesosilver (TM) and AgC, in male mice. Both products had similar silver accumulation after high-dose sub-chronic exposure. Physicochemical modifications of silver nanoparticles and ions were observed in the gut. Both products had a moderate impact on the gut barrier at lower doses. The composition of gut microbiota was altered differently by each product. Near-infrared spectroscopy successfully distinguished between the two exposures. This study demonstrates the applicability of biophysics-based methods in understanding the uptake, fate, and toxicological effects of colloidal silver after oral exposure.
ENVIRONMENTAL SCIENCE-NANO
(2023)
Review
Microbiology
Lucie Etienne-Mesmin, Benoit Chassaing, Mickael Desvaux, Kim De Paepe, Raphaele Gresse, Thomas Sauvaitre, Evelyne Forano, Tom Van de Wiele, Stephanie Schuller, Nathalie Juge, Stephanie Blanquet-Diot
FEMS MICROBIOLOGY REVIEWS
(2019)
Letter
Gastroenterology & Hepatology
O. Inczefi, V. Bacquie, M. Olier-Pierre, M. Rincel, B. Ringot-Destrez, S. Ellero-Simatos, H. Eutamene, C. Betoulieres, J. Thomas, J. Laine, L. Gros, M. Leveque, R. Leonard, C. Harkat, C. Robbe-Masselot, R. Roka, M. Mercier-Bonin, V. Theodorou, M. Darnaudery, J. R. Turner, L. Ferrier
CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY
(2020)