Review
Infectious Diseases
Katie M. Cronin, Michael A. Moso, Eddie Chan, Katherine A. Bond
Summary: The review highlights the differences in methodology and breakpoints for detecting penicillin and oxacillin resistance in S. lugdunensis between EUCAST and CLSI. There is also concern about emerging oxacillin resistance in some geographical areas, particularly in mecA positive isolates.
CURRENT OPINION IN INFECTIOUS DISEASES
(2022)
Article
Microbiology
Joanne S. K. Teh, Ioanna Pantelis, Xiao Chen, Tania Sadlon, Kelly Papanaoum, David L. Gordon
Summary: The evaluation of penicillin and oxacillin susceptibility testing on 200 Staphylococcus lugdunensis isolates was conducted. The results showed that the disc diffusion method with zone edge interpretation was more accurate and specific compared to automated broth microdilution for S. lugdunensis.
JOURNAL OF CLINICAL MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Maja Kosecka-Strojek, Mariola Wolska-Gebarzewska, Adrianna Podbielska-Kubera, Alfred Samet, Beata Krawczyk, Jacek Miedzobrodzki, Michal Michalik
Summary: This study characterized 18 strains of S. lugdunensis from maxillary sinuses, identifying several virulence factors associated with chronic disease and demonstrating that most strains were susceptible to antibiotics, with some showing resistance to fosfomycin, erythromycin, and clindamycin. Further analysis of S. lugdunensis strains from various sources, including the nasal cavity, is recommended to enhance understanding of this opportunistic pathogen and reduce infection rates.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Rosa Fernandez-Fernandez, Carmen Lozano, Laura Ruiz-Ripa, Beatriz Robredo, Jose Manuel Azcona-Gutierrez, Carla Andrea Alonso, Carmen Aspiroz, Myriam Zarazaga, Carmen Torres
Summary: This study characterized 56 S. lugdunensis isolates from two Spanish hospitals, assessing their antimicrobial susceptibility, resistance genes, and ability to inhibit indicator bacteria. A portion of the isolates exhibited inhibition against multidrug-resistant strains, including antimicrobial producer strains carrying the lugD gene.
Article
Immunology
Denny Chin, Ronald S. Flannagan, Stephen W. Tuffs, Jeremy K. Chan, John K. McCormick, David E. Heinrichs
Summary: This study used random transposon insertion mutagenesis and RNA sequencing to discover that the agr quorum sensing system regulates the expression of virulence factors in S. lugdunensis. The agr-regulated effectors are important for the survival of S. lugdunensis in whole human blood and during encounters with macrophages. This research provides insight into the strategies used by this bacterium to cause disease.
INFECTION AND IMMUNITY
(2022)
Article
Microbiology
Pak-Leung Ho, Ying-Hang Law, Melissa Chun-Jiao Liu, Andes Lau, Man-Ki Tong, Kin-Hung Chow, Alan Ka-Lun Wu, Cindy Wing-Sze Tse, Vincent Chi-Chung Cheng, Tak-Lun Que
Summary: The new oxacillin agar reliably differentiated mecA-positive and mecA-negative isolates in S. lugdunensis, while cefoxitin DD and MIC tests showed limitations in reliable detection of some mecA-positive S. lugdunensis isolates.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Infectious Diseases
Nisa Sipahi, Ertugrul Kaya, Cansu Celik, Orhan Pinar
Summary: This study aimed to identify staphylococcal species in raw milk and determine their hemolysis, biofilm, coagulase activities, and β-lactam resistance. The results showed that these staphylococcal species exhibited certain virulence factors and antibiotic resistance genes, highlighting the need for continuous monitoring and attention to resistance concerns.
Article
Evolutionary Biology
Joshua T. Smith, Cheryl P. Andam
Summary: Members of the genus Staphylococcus have been historically classified into coagulase-positive and coagulase-negative based on the presence of the coagulase protein. While horizontal gene transfer has been well-studied in CoPS species like Staphylococcus aureus, this study sheds light on the importance of these processes in CoNS evolution. CoNS species serve as reservoirs of transferrable genes associated with antimicrobial resistance, with certain species acting as hubs of gene flow and genetic diversity for the entire genus.
GENOME BIOLOGY AND EVOLUTION
(2021)
Article
Infectious Diseases
Hisham N. Altayb, Hana S. Elbadawi, Othman Baothman, Imran Kazmi, Faisal A. Alzahrani, Muhammad Shahid Nadeem, Salman Hosawi, Kamel Chaieb
Summary: This study investigated the genomic characteristics of methicillin-resistant Staphylococcus epidermidis isolated from clinical specimens. The isolates showed multidrug resistance and carried various resistance genes. One isolate belonged to the globally disseminated ST2 strain and harbored biofilm-formation genes and a resistance gene associated with device-related infections.
Review
Microbiology
Bohdan L. Bilyk, Viralkumar V. Panchal, Mariana Tinajero-Trejo, Jamie K. Hobbs, Simon J. Foster
Summary: The development of resistance to beta-lactam antibiotics has made Staphylococcus aureus, especially MRSA, a global clinical burden. The acquisition of mecA, which encodes PBP2A, allows MRSA to proliferate in the presence of beta-lactams. Further genetic adjustments are required to develop high-level resistance.
MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS
(2022)
Article
Microbiology
Clara Locatelli, Stefano Gattolin, Valentina Monistero, Bianca Castiglioni, Paolo Moroni, Maria Filippa Addis, Paola Cremonesi
Summary: Accurate and precise differentiation of staphylococci isolated from milk is crucial for managing udder health, especially the rapid and specific identification of Staphylococcus aureus for preventing and treating bovine mastitis. However, rarely occurring coagulase-deficient S. aureus strains may cause clinical and subclinical mastitis cases. By studying S. aureus isolates from a single herd over 10 years, we found the persistence of a coagulase-negative S. aureus strain due to a single base pair deletion in the coa gene sequence. It highlights the importance of integrating biochemical and molecular/sequence analysis methods to correctly identify and discriminate atypical S. aureus strains in bovine herds.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Chemistry, Analytical
Xiaolin Xie, Xiaoxue Cheng, Juan Dong, Jia Li, Lanxin Jiang, Tiantian Yang, Bing Liao, Shijia Ding, Qingsong Liu, Fukang Luo, Wei Cheng, Junman Chen
Summary: A visual assay based on isothermal rolling circular amplification and G-quadruplex/hemin DNAzyme proximity assembly was proposed for the efficient detection of methicillin-resistant Staphylococcus aureus (MRSA). The assay showed high sensitivity and specificity, with a limit of detection of 9.6 pM. Compared to classic methods, this colorimetric strategy offers simplified procedures and shorter turnaround time.
ANALYTICAL CHEMISTRY
(2023)
Article
Medicine, General & Internal
Aynur Veliev, Yasar Nakipoglu
Summary: This study aimed to investigate S. lugdunensis and selected CNS strains using a simple biochemical method and determination of their antibiotic susceptibilities. The results showed that ODC and PYR tests can be used for accurate identification of S. lugdunensis and eliminating opportinistic pathogens from other CNS species.
PAKISTAN JOURNAL OF MEDICAL SCIENCES
(2022)
Article
Microbiology
K. H. Muneeb, S. Sudha, G. K. Sivaraman, Bibek Shome, Jennifer Cole, Mark Holmes
Summary: This study revealed high levels of antibiotic resistance among staphylococci, with all isolates resistant to beta-lactam antibiotics and a significant proportion resistant to non-beta-lactam antibiotics. Multiple drug resistance was observed in over half of the isolates, with virulence factors predominantly found in S. haemolyticus. The study also raised concerns about the potential dissemination of resistant genes to other staphylococci species.
ARCHIVES OF MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Hend K. Sorour, Azhar G. Shalaby, Marwa A. Abdelmagid, Reham A. Hosny
Summary: This study aimed to investigate the pathogenic potential of vancomycin and methicillin-resistant coagulase-negative Staphylococci (VMRCoNS) on Egyptian poultry farms. The results showed the presence of various species of CoNS in imported poultry flocks and commercial poultry farms, which exhibited resistance to multiple antibiotics and pathogenicity. Therefore, special attention should be given to the public health impact of these strains.
INTERNATIONAL MICROBIOLOGY
(2023)